26 results about "Animal Organs" patented technology

The invention belongs to the field of biological medicine, and provides a protection liquid capable of preserving in-vitro animal organs or in-vitro animal tissues at normal temperature as well as a preparation method and application of the protection liquid, and the protection liquid comprises a solution containing a hemoglobin oxygen carrier or a hemoglobin carrier, an osmotic pressure regulating substance and the like. The protective liquid provided by the invention can be used for preserving or perfusing broken limbs, isolated animal organs or isolated animal tissues at normal temperature, and can also be used as a culture liquid of artificial organs and artificial tissues.

The invention relates to the technical field of biology, in particular to a chicken CKAP4 protein polyclonal antibody and application and a kit thereof. An animal is immunized with the CKAP4 recombinant protein, serum is purified from the immunized animal, the chicken CKAP4 protein polyclonal antibody is obtained, and the amino acid sequence of the CKAP4 recombinant protein is shown as SEQ ID No.1. Sensitivity and specificity detection tests on the chicken CKAP4 protein polyclonal antibody find that the serum titer of CKAP4 is 1: 102400, the expression of the CKAP4 protein mainly focuses on cytoplasm parts of various organs of animals, and the polyclonal antibody can specifically bind to the CKAP4 protein in animal tissues. Therefore, the polyclonal antibody has the advantages of high sensitivity, good specificity, wide application range and the like, and a foundation is laid for later detection of CKAP4 protein and research on pathological mechanisms of virus infection diseases.

The utility model belongs to the technical field of organ transplantation auxiliary equipment, and particularly relates to an animal organ perfusion system and equipment. The animal organ perfusion system comprises a temperature control pipeline loop and at least one perfusion pipeline loop. The perfusion pump, the filter, the oxygenator, the bubble remover, the flow sensor, the pressure sensor and the organ chamber are sequentially connected through the perfusion pipeline. The temperature control pipeline loop comprises a circulating liquid pipeline, and a temperature control device, a temperature sensor, a circulating pump and a bathtub which are sequentially connected through the circulating liquid pipeline. Wherein the bathtub is used for containing circulating liquid, the temperature control pipeline loop is used for regulating and controlling the temperature of the circulating liquid, and the organ chamber is arranged in the bathtub so as to be capable of exchanging heat with the circulating liquid. The animal organ perfusion equipment provided by the utility model is provided with the animal organ perfusion system so as to realize automatic perfusion of organs of small animals. The device is relatively miniaturized, the manufacturing cost is relatively low, and the cost of the small animal organ perfusion experiment is relatively low.

The invention relates to the technical field of biochemical substance separation and concentration, and discloses a concentration method of biochemical substances. According to the method, the technical target is realized through seven systematic steps: preparing a biocompatible deep eutectic solvent, constructing a water phase-DES two-phase system, preparing a phase antagonistic biosurfactant mixed system, dynamically regulating and controlling an interface, sequentially migrating and separating biochemical substances, performing multi-stage enrichment and collection, and recycling DES. According to the method, through innovative regulation and control on the interface property and the time dimension, the problem of high-selectivity separation of tiny biochemical substances with polarity difference is effectively solved, the separation purity and the recovery rate are remarkably improved, meanwhile, the biological activity is kept, and a green and sustainable technical solution is provided for efficient concentration of sensitive biochemical substances; the concentration method of the biochemical extract comprises the following steps: taking animal organs or tissues without human beings, and extracting bioactive components in the animal organs or tissues to be used for preparing medicines for treating or preventing cardiovascular and cerebrovascular diseases.

A method of imaging vascular activity at microscopic scale in at least one region of a network of blood vessels of a human or animal organ, the method comprising: (a) emitting a series of consecutive incident ultrasound waves in the at least one region by an ultrasound transducer array extending in at least one direction, the incident ultrasonic waves are propagated in the direction perpendicular to the transducer array; (b) acquiring a set of raw data from the backscattered ultrasound by the transducer array; (c) generating a series of continuous ultrasound images according to the original data; (d) detecting at least one isolated ultrasound contrast agent in the ultrasound image; (e) locating the position of the at least one isolated ultrasound contrast agent at a precision lower than the wavelength of the wave; (f) generating an at least two-dimensional backscatter amplitude image by assigning, to each pixel, a value representative of the measured backscatter amplitude of the at least one isolated ultrasound contrast agent detected in that pixel.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.

1. Name of the product in this design: Live Imaging Organ Fixation Instrument. 2. Purpose of this design: To fix organs or tissues of live experimental animals during microscopic imaging. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

A cooked chunk pet food product is prepared by grinding animal muscle meat and animal organ meat and mixing the animal muscle meat and the animal organ meat in a ratio of muscle meat to organ meat of from about 1:1 to about 10:1 wt % in a mixer for from about 5 to 20 minutes to form a mixed meat component, mixing with additional ingredients to prepare a pet food base formulation that is disposed in a casing and cooked to provide a sliceable pet food log. The pet food log is then diced and / or shredded provide a chunk pet food product having an average piece size of from about 0.25 to about 0.75 inches in the largest dimension. Products made by the process are also described.

Systems and methods are provided for decision support in toxicology assessment. The system includes a processor and a non-transitory computer readable medium storing instructions executable by the processor to provide an imager interface that receives an image of organ tissue of an animal and a vision encoder trained on a plurality of training images that receives the image of organ tissue and provides a representation of the image of organ tissue. Each training image includes an image of organ tissue of an animal. A toxicology front end generates one of a value representing lesions within the image of organ tissue or an image having a similar representation from the representation of the image of organ tissue.

To provide a reinforced decellularized and recellularized artificial organ graft by providing a method for sealing the decellularized and recellularized artificial organ graft.SOLUTION: A method of reducing the porosity of an outer fibrous layer of a decellularized mammalian organ or tissue or portion thereof, the method comprising contacting an exterior surface of a mammalian organ or tissue or portion thereof comprising a vascular tree and cells with a composition comprising at least one agent in an amount to form a coating on the exterior surface, wherein: Wherein the coating is effective to increase the retention of fluid in the organ or tissue relative to fluid introduced into the corresponding organ or tissue or portion thereof prior to exposure to the composition, and decellularizing the coated mammalian organ or tissue or portion thereof by perfusion decellularization.SELECTED DRAWING: None

Provided herein are methods and compositions related to at least partially recellularizing human organs. Various methods of decellularizing organs of a non-human animal and re-cellularizing extracellular matrix of a non-human animal with a cellular composition. Compositions and methods for treating liver or other diseases, such as acute liver failure, using ex vivo bioengineered liver or other organs are also provided.

The invention belongs to the technical field of pathogenic microorganism detection, and particularly relates to a quadruple fluorescent quantitative PCR detection method for PEDV, TGEV, PoRV and PDCoV. According to the invention, four pairs of specific primers and probes are designed according to the difference of whole genome sequences of PEDV, TGEV, PoRV and PDCoV, and a quadruple fluorescent quantitative PCR detection method is established. The detection method has good specificity, sensitivity and repeatability, can be used for detecting low-micro-content samples such as organs, blood, mouth and nose swabs and environmental samples of animals, and provides a new tool for preventing spreading of PEDV, TGEV, PoRV and PDCoV in swinery.

Through experimental studies, the present invention discovered that GPR180 is highly expressed in animal organs, particularly the liver, and that GPR180 gene expression in the liver is upregulated under the pathological conditions of obesity and diabetes in mice. After knocking down GPR180 in the liver, energy metabolism was enhanced and body fat content was reduced in animals on both a normal diet and a high-fat diet. Knocking down GPR180 in the liver also improved fatty degeneration of the liver in animals induced by a high-fat diet, reduced cholesterol levels in plasma and liver, and improved insulin resistance and increased insulin sensitivity in animals on both a normal diet and a high-fat diet. Based on this, GPR180 can be used as a therapeutic target for non-alcoholic fatty liver disease, weight loss or fat reduction, treatment of diabetes, and hypercholesterolemia. GPR180 inhibitors can be used to prepare drugs for treating non-alcoholic fatty liver disease, weight loss or body fat reduction, obesity, diabetes, and hypercholesterolemia.

The utility model relates to a pathological dissecting device for animal organ tissues, which belongs to the field of pathological dissecting devices and comprises a water tank and a drainage pipe thereof, a movable groove is arranged on the water tank, a bedplate in a net structure is arranged on the movable groove, the movable groove is of a structure which is gradually narrowed towards the central part from top to bottom, and a drainage port is arranged on a bottom plate of the movable groove. A sliding table used for bearing the movable groove and a sliding rail frame used for supporting the sliding table are arranged in the water tank, and a supporting base matched with the sliding table is rotationally installed below the movable groove. According to the animal organ tissue pathological dissecting device, the whole formed by connecting the movable groove and the table plate is used for containing an object to be dissected and collecting flushing waste water generated in the dissecting process, and the movable groove is provided with the adjustable illuminating lamp and the flushing pipe, so that an operator can adjust the dissecting position and the illuminating range according to operation requirements; and the dissected part can be conveniently and rapidly washed, and the operation convenience is comprehensively improved when organ tissue pathological dissection is conducted on the animal.

A method (100) of providing an automated diagnostic aid The method comprises the steps of providing scan data comprising image data, segmenting the scan data (104) to identify different regions of the scanned organ, meshing the segmented scan data (106) to provide a meshed CAE model of the at least part of the human or animal body organ and applying properties and boundary conditions (108) to the meshed CAE model. The meshed CAE model is solved (110) and used as a diagnostic tool. At least one of the steps of segmenting, meshing and applying properties and boundary conditions is carried out by a reinforcement learning trained agent comprising an artificial neural network.

This invention relates to a method for fusing and reconstructing multi-view anisotropic three-dimensional images, comprising: acquiring three orthogonal MRI images of a small mammalian organ; aligning the spatial proportions of the three orthogonal MRI images to obtain images I1, I2, and I3; extracting feature points on a reference image R1; calculating the affine transformation matrix of the reference image and the floating image using the correspondence between two sets of feature point pairs corresponding to the obtained reference image and the floating image; obtaining two generated images R2 and R3; obtaining three images R1', R2', and R3' after brightness adjustment; and obtaining a three-dimensional isotropic image, i.e., the final output image I. o This invention precisely stitches and aligns three images from different perspectives. By fusing and reconstructing multiple orthogonal low-resolution interlayer images, the clarity of the originally low-resolution images is greatly improved, providing high-definition image data support for the study of organ function in small mammals.

The present invention relates to method of assessing the performance of a human or robot carrying out a medical procedure by using a phantom resembling a human or animal organ or tissue and to an assessment tool comprising such a phantom.

The present application relates to a protein for regulating the activity of a mitochondrial calcium channel and its application in preventing and treating obesity and related diseases, and belongs to the field of adipose tissue and related metabolism. The technical problem to be solved by the present application is how to prevent and / or treat obesity or obesity-related diseases. To solve the above technical problem, the present application provides a protein, which is an EMRE-UCP1 protein, wherein the EMRE-UCP1 protein is a protein fused from a mitochondrial calcium ion transport regulatory protein and a uncoupling protein-1. To solve the above technical problem, the present application provides a method for drug screening, wherein the method comprises using the transgenic animal cell line, or the transgenic animal tissue, or the transgenic animal organ of B11) to perform drug screening, and the drug is a drug for preventing and / or treating obesity or obesity-related diseases.

Methods and compositions relating to at least partially recellularized human organs are provided herein. Various methods for decellularizing non-human animal organs and recellularizing the non-human animal extracellular matrix with cellular compositions. Furthermore, compositions and methods for treating liver disease or other diseases (such as acute liver failure) using an extracorporeal bioartificial liver or other organs are provided herein.

The invention relates to a system and method for quantifying a novel biomarker of the tissue activity of a human or animal organ. By way of preferred application, such a biomarker describes the diffusivity of biological fluids in living tissues in the form of a novel indicator of the diffusion of water molecules in living tissues on the basis of diffusion data resulting from the acquisition of a sequence of images of one or more parts of the body of an animal or human patient. Particularly resistant and stable with respect to noise present in the medical imaging signals from which the experimental data stem, the novel biomarker is relevant in a large number of applications including, inexhaustively, the analysis and / or monitoring of cancers, or the assessment of strokes.

An organ tissue composition or constitution assessment apparatus (10) comprises first and second panel elements (16, 18) disposed in spaced apart and facing relationship. One of the first and second panel elements (16, 17) is adjustable relative to the other in distance. At least one ultrasound emitter (32) is disposed on the second panel element (18). At least one ultrasound receiver (30) is disposed on the first panel element (16), such that the emitter and receiver face another. The panel elements are connected to a support structure (12, 14) and preferably in an arrangement which enables assessment of a patient's body part while the patient is in an upright position. The apparatus includes a processing unit (304) which is configured to determine from the received ultrasound signals a tissue composition or constitution measure associated with the organ tissue to be assessed and preferably an overall tissue composition or constitution measurement across the entire area of the organ tissue to be assessed. The preferred embodiment is able to obtain a measure of an average breast tissue composition or constitution, which can be used as indicative of a medical condition or risk of a medical condition, such as cancer, and as a guide to selection of optimum screening procedures.

The utility model relates to a heating and storage module and a perfusion device for an in-vitro animal organ. The heating and storage module comprises an organ bin, a water bath bin, a heating assembly and a temperature detection module, the organ bin is used for containing in-vitro animal organs and perfusate, so that the in-vitro animal organs are immersed in the perfusate; the water bath bin can store a liquid medium and is detachably accommodated in the organ bin; the heating assembly is arranged at the bottom of the water bath bin and is used for heating the water bath bin, so that the heat is conducted to the organ bin after the liquid medium stored in the water bath bin is heated; the temperature detection module can be used for detecting the temperature of the liquid medium in the water bath bin. The perfusion device comprises the heating and storage module, a perfusion module and a main control module, the perfusion module is used for providing power for perfusate, and the main control module is in communication connection with the heating assembly, the temperature detection module and the perfusion module so as to control the temperature and the perfusion pressure. Compared with the prior art, the integration of the heating module and the storage module is greatly improved, the size is reduced, and the perfusion device can ensure the accuracy of experimental data at an ideal test temperature.

This application relates to the technical field of biomimetic materials, specifically disclosing a conductive, low-pollution simulation material for laparoscopic surgery training and its preparation method. The conductive, low-pollution simulation material for laparoscopic surgery training comprises the following components in parts by weight: 2.9-3.9 parts hydrophilic colloid; 0.4-0.6 parts leavening agent; 0.05-0.15 parts crosslinking agent; 0.2-0.25 parts N,N,N',N'-tetramethylethylenediamine; and 65 parts water; wherein the hydrophilic colloid is a mixture of gelatin and carrageenan. This conductive, low-pollution simulation material for laparoscopic surgery training can be used in robotic surgery training, replacing traditional materials such as silicone and animal organs. It has the advantages of being conductive, having a softness and hardness close to human tissue, and not causing surgical contamination. Furthermore, the preparation method of this application has the advantages of fewer steps, simplicity, and high efficiency, which is conducive to the industrial-scale production of simulation materials.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.

The invention provides a blood vessel quick connection structure for experimental animal organ transplantation, and relates to the field of animal experiment instruments. The blood vessel quick connection structure for experimental animal organ transplantation comprises a sleeve and a sleeve handle arranged at one end of the sleeve, and the sleeve is of a hollow columnar structure; the sleeve handle is of an arc-shaped sheet structure with the same radian as the sleeve, and one end of the sleeve handle is connected with part of the circumference of one end of the sleeve. A stable supporting point can be provided for extroversion and knotting of the blood vessel during blood vessel connection, so that the blood vessel can be rapidly and stably connected during an operation, the operation is simple and convenient, and the operation efficiency is greatly improved.

The invention discloses a ruminant stomach recovery two-way flushing device and method, and belongs to the technical field of animal organ dissecting instruments, the device comprises a workbench, and the bottom of the workbench is connected with supporting legs; a flushing cylinder is fixed to the upper surface of the workbench. The top of the flushing cylinder is connected with a cylinder cover; the cylinder cover is connected with a piston through an operating rod; two hard ear tubes are connected to the side face of the flushing cylinder, the ends of the two hard ear tubes are in butt joint with soft conveying tubes respectively, the ends of the two soft conveying tubes are in butt joint with hard intubation tube assemblies respectively, and the two hard intubation tube assemblies are inserted into the cardia and the pylorus of the compound stomach respectively. Flushing fluid is injected from the cardia and the pylorus at the same time, bidirectional flushing of the stomach is achieved, water pressure is increased through piston pressurization, and crude fibers in the rumen can be effectively removed; secondly, a hedging effect can be generated in the stomach recovery process through two-way flushing, chyme particles attached to the surface of the mucous membrane can be effectively cleaned, and the overall integrity of the gastric mucous membrane can be guaranteed while it is guaranteed that the gastric mucous membrane is thoroughly flushed clean.