Perfusion device and perfusion method for isolated animal organ

Abstract

The embodiment of the invention relates to medical equipment, in particular to an in-vitro animal organ perfusion device and method, and the perfusion device comprises a heating and storage module, a perfusion module and a main control module; the heating and storage module is used for containing the isolated animal organs and perfusate and heating the contained isolated animal organs; the perfusion module is used for providing power for the perfusate in the heating and storage module, so that the perfusate circulates in the in-vitro animal organ; the main control module is respectively in communication connection with the heating and storage module and the perfusion module, and is used for acquiring the pressure when the perfusion liquid circulates in the in-vitro animal organ in real time and controlling the perfusion module according to the acquired pressure; the main control module further obtains the temperature of the heating and storage module for heating the in-vitro animal organ in real time and controls the heating and storage module according to the obtained temperature. Compared with the prior art, when extracorporeal circulation is established for the in-vitro animal organ, experimenters do not need to monitor parameters such as pressure and temperature.

Description

Technical Field

[0001] Embodiments of the present invention relate to a medical device, and particularly to a perfusion device and perfusion method for an ex vivo animal organ. Background Art

[0002] Organ transplantation is the most effective method for treating end-stage diseases of various organs. With the development of the human economy and the progress of science and technology, more and more patients suffering from end-stage organ diseases have joined the transplant list waiting for organ transplantation. Currently, there are more than 1.5 million patients with organ failure in China every year, and the best way to save these patients' lives is organ transplantation. In recent years, the organ transplantation technology in China has developed rapidly, and the annual number of organ transplantation surgeries currently ranks second in the world, with a broad market prospect for transplantation. However, although the transplantation technology has developed rapidly and the number of surgeries has gradually increased, the problem of shortage of donor organ resources is still very serious. To solve the problem of shortage of transplanted organs, more and more marginal donor (ECD) organs have been included in the scope of consideration for transplantation. However, in the whole process of organ acquisition and transplantation in conventional organ transplantation surgeries, the organ will suffer from ischemia-reperfusion injury (IRI). Ischemia-reperfusion injury will cause the activation of a large number of inflammatory cells and the release of a large number of oxygen free radicals, thereby causing damage to the organ. High-quality donor organs can tolerate the damage caused by ischemia-reperfusion. However, for marginal standard donors (ECD), ischemia-reperfusion injury (IRI) is a devastating blow, which will lead to a series of complications such as early allograft dysfunction (EAD), biliary complications, acute rejection, and primary non-function of the graft (PNF) in patients after surgery, and even lead to the death of the patient. Therefore, the traditional cold preservation method is not applicable to marginal donor organs. In recent years, in order to effectively evaluate the quality of marginal donor organs in vitro and reduce the risk of complications in transplant patients, the mechanical perfusion technology has been continuously developed. Mechanical perfusion (MP) refers to perfusing the organ blood vessels with a perfusion fluid in a continuous or pulsed manner through mechanical power and forming a circuit to achieve the purpose of preserving the organ in vitro. The invention of the mechanical perfusion technology has made it possible to study organs ex vivo. Currently, normothermic mechanical perfusion technology has been proven to be able to effectively evaluate the quality of organs in vitro and reduce the complications in patients after surgery.

[0003] The development of normothermic mechanical perfusion technology has made it possible to extend the preservation time of organs and evaluate organ quality in vitro, but there are still many marginal organs (including fatty degeneration organs, DCD donor organs, elderly donor organs, infected organs, etc.) that are not used and eventually abandoned. Therefore, how to use normothermic mechanical perfusion technology to repair damaged organs and expand the donor pool or use normothermic mechanical perfusion technology to treat diseased organs in vitro has great scientific research and clinical prospects. In order to promote the progress of mechanical perfusion technology and promote the development of research on the repair and treatment of damaged diseased organs or diseased organs, a large number of organ models are needed. Due to the precious resources of donor organs and the lack of donor organs, research based on in vitro treatment cannot be effectively carried out on donor organs. The traditional scientific research method is based on cell experiments and animal experiments. Since cells cannot be perfused in vitro, animal models are the most effective way for us to expand normothermic mechanical perfusion technology and conduct research on the repair and treatment of damaged organs in vitro. At the same time, the animal normothermic mechanical perfusion system can not only be used in the field of transplantation research, but also can realize multidisciplinary cross-disciplinary research through the animal perfusion platform, helping drug development, drug screening, genetic engineering, and material engineering.

[0004] Animal perfusion research is mainly based on pigs, rats and mice. The cost of perfusion experiments using pig organs is expensive. Before the experiment begins, the pigs need to be intubated and maintained on a ventilator. Professional anesthesiologists are needed to maintain the pigs' vital signs. Organ removal surgery requires professional surgeons, and the cost of raising pigs is high. Therefore, using pigs for perfusion experiments consumes a lot of manpower, material resources and financial resources, and they are not ideal experimental research animals.

[0005] For organ perfusion research, rats and mice are suitable experimental animals. The preliminary demonstration of any scientific research cannot be separated from experimental mice. The research on rats and mice has a complete scientific system. At the same time, rats and mice are simple to raise, reproduce quickly, and the surgical operation is not difficult. 2 to 3 experimenters can cooperate with each other to complete the experimental operation. However, the existing small animal perfusion equipment is mainly made by scientific researchers. The perfusion equipment is fragile and inconvenient to clean. The assembly before perfusion is cumbersome, which easily increases the risk of perfusion fluid contamination. The perfusion process requires experimenters to monitor and adjust parameters such as perfusion pressure and temperature in real time, which greatly reduces the efficiency of the experiment. In addition, the current perfusion equipment implements single-channel portal vein perfusion when conducting research on rat livers, which is inconsistent with the physiological conditions of the liver and cannot completely simulate the physiological environment of the organ in vitro. Summary of the invention

[0006] The object of the embodiments of the present invention is to design a perfusion device and a perfusion method for an ex vivo animal organ, which can not only simulate the physiological environment of various ex vivo animal organs in vitro, but also, when establishing extracorporeal circulation for the ex vivo animal organ, eliminate the need for experimenters to monitor parameters such as perfusion pressure and temperature, thereby greatly saving manpower and improving experimental efficiency.

[0007] To achieve the above object, an embodiment of the present invention provides a perfusion device for an ex vivo animal organ, comprising:

[0008] A heating and storage module for containing the ex vivo animal organ and the perfusion fluid, and for heating the contained ex vivo animal organ;

[0009] A perfusion module for providing power to the perfusion fluid in the heating and storage module to make the perfusion fluid continuously circulate through the ex vivo animal organ;

[0010] A main control module communicatively connected to the heating and storage module and the perfusion module respectively;

[0011] Wherein, the main control module is used to obtain in real time the perfusion pressure when the perfusion fluid circulates through the ex vivo animal organ, and control the perfusion module according to the obtained perfusion pressure;

[0012] The main control module is further used to obtain in real time the temperature when the heating and storage module heats the ex vivo animal organ, and control the heating and storage module according to the obtained temperature.

[0013] In addition, an embodiment of the present invention further provides a perfusion method for an ex vivo animal organ. The perfusion method is applied to the perfusion device as described above, and the perfusion method comprises the following steps:

[0014] When power is provided to the perfusion fluid in the heating and storage module to make the perfusion fluid continuously circulate through the ex vivo animal organ, the main control module obtains in real time the perfusion pressure when the perfusion fluid circulates through the ex vivo animal organ, and obtains in real time the temperature when the heating and storage module heats the ex vivo animal organ;

[0015] The main control module controls the perfusion module according to the obtained perfusion pressure, and controls the heating and storage module according to the obtained temperature.

[0016] Compared with the prior art, the embodiments of the present invention can not only hold the excised animal organ and the perfusion fluid through the heating and storage module of the perfusion device, but also heat the excised animal organ. At the same time, since the perfusion module can also provide power to the perfusion fluid contained in the heating and storage module, enabling the perfusion fluid to continuously circulate through the excised animal organ, an extracorporeal circulation can be established for the excised animal organ, thus eliminating many potential contamination risk operations. In addition, because the main control module can obtain the perfusion pressure in real time when the perfusion fluid circulates through the excised animal organ, and obtain the temperature in real time when the heating and storage module heats the excised animal organ, and control the perfusion module according to the obtained perfusion pressure, and control the heating and storage module according to the obtained temperature, when establishing an extracorporeal circulation for the excised animal organ, it is not necessary for experimenters to monitor parameters such as perfusion pressure and temperature, thus greatly saving manpower and improving experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 An isometric schematic view of the perfusion device for an excised animal organ in some embodiments of the present invention;

[0018] Figure 2 A side view schematic of the perfusion device for an excised animal organ in some embodiments of the present invention;

[0019] Figure 3 An exploded schematic view of the heating and storage module in some embodiments of the present invention;

[0020] Figure 4 A system module block diagram of the perfusion device for an excised animal organ in some embodiments of the present invention;

[0021] Figure 5 A flowchart schematic of the perfusion method for an excised animal organ in some embodiments of the present invention;

[0022] Figure 6 A flowchart schematic when the main control module controls the perfusion module in some embodiments of the present invention;

[0023] Figure 7 A flowchart schematic when the main control module controls the heating and storage module to heat the excised animal organ in some embodiments of the present invention;

[0024] Figure 8 A top view schematic of the perfusion device for an excised animal organ in some embodiments of the present invention;

[0025] Figure 9 is Figure 8 the cross-sectional view at A-A in DETAILED DESCRIPTION OF THE EMBODIMENTS

[0026] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the following will elaborate on each embodiment of the present invention with reference to the accompanying drawings. However, those of ordinary skill in the art can understand that in each embodiment of the present invention, many technical details are provided to help readers better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical solutions claimed in the present application can still be implemented.

[0027] Embodiment 1

[0028] The first embodiment of the present invention relates to a perfusion device for an ex vivo animal organ, as Figure 1 and Figure 4 shown, comprising: a heating and storage module 1, a perfusion module 2, and a main control module 3.

[0029] Among them, as combined with Figure 3 shown, the heating and storage module 1 is used to hold the ex vivo animal organ and the perfusion fluid, and is used to heat the held ex vivo animal organ. Secondly, as Figure 1 and Figure 2 shown, the perfusion module 2 is used to provide power to the perfusion fluid in the heating and storage module 1, so that the perfusion fluid continuously circulates through the ex vivo animal organ.

[0030] In addition, as Figure 4 shown, the main control module 3 is respectively communicatively connected to the heating and storage module 1 and the perfusion module 2, and the main control module 3 is used to obtain in real time the perfusion pressure when the perfusion fluid circulates through the ex vivo animal organ, and can control the perfusion module 2 according to the obtained perfusion pressure. At the same time, the main control module 3 is also used to obtain in real time the temperature when the heating and storage module 1 heats the ex vivo animal organ, and can control the heating and storage module 1 according to the obtained temperature.

[0031] Furthermore, the main control module 3 is also used to record in real time the perfusion pressure data when the perfusion fluid circulates through the ex vivo animal organ, and the temperature data when the heating and storage module 1 heats the ex vivo animal organ. At the same time, the main control module 3 is also used to export the recorded perfusion pressure data and temperature data.

[0032] It is not difficult to find from the above that the heating and storage module 1 of the perfusion device can not only hold the excised animal organ and the perfusion fluid, but also heat the excised animal organ. At the same time, since the perfusion module 2 can also provide power to the perfusion fluid contained in the heating and storage module 1, enabling the perfusion fluid to continuously circulate through the excised animal organ, an extracorporeal circulation can be established for the excised animal organ, thus eliminating many potential contamination risk operations. In addition, because the main control module 3 can obtain the perfusion pressure in real time when the perfusion fluid circulates through the excised animal organ, and obtain the temperature in real time when the heating and storage module 1 heats the excised animal organ, and control the perfusion module according to the obtained perfusion pressure, and control the heating and storage module according to the obtained temperature, when establishing an extracorporeal circulation for the excised animal organ, there is no need for experimenters to monitor parameters such as perfusion pressure and temperature, thus greatly saving manpower and improving the experimental efficiency.

[0033] Specifically, in some embodiments, such as Figure 1 and Figure 4 shown, the perfusion module 2 includes: a peristaltic pump 21 and a pressure detection module 22. Among them, the perfusion pipeline (not marked in the figure) of the peristaltic pump 21 is inserted into the heating and storage module 1, so that the peristaltic pump 21 can provide power to the perfusion fluid in the heating and storage module 1 through the perfusion pipeline, enabling the perfusion fluid to continuously circulate through the excised animal organ. Secondly, in some embodiments, in combination with Figure 4 shown, the pressure detection module 22 can adopt a pressure sensor, and this pressure sensor is used to detect the perfusion pressure when the perfusion fluid circulates through the excised animal organ. And, as Figure 4As shown, the main control module 3 is respectively communicatively connected to the peristaltic pump 21 and the pressure detection module 22. The main control module 3 is used to obtain the perfusion pressure measured by the pressure detection module 22. At the same time, the main control module 3 can also compare the obtained perfusion pressure with the target pressure, and can control the rotation speed of the peristaltic pump 1 according to the comparison result. For example, when the perfusion module 2 provides power to the perfusion fluid, it can be divided into two modes, namely, the automatic perfusion mode and the manual perfusion mode. When the automatic perfusion mode is adopted, when the perfusion pressure obtained by the main control module 3 is greater than the target pressure, it indicates that the perfusion pressure when the perfusion fluid circulates in the ex vivo animal organ is too high. At this time, the main control module 3 can reduce the rotation speed of the peristaltic pump 21. On the contrary, when the perfusion pressure obtained by the main control module 3 is less than the target pressure, it indicates that the perfusion pressure when the perfusion fluid circulates in the ex vivo animal organ is too low. At this time, the main control module 3 can increase the rotation speed of the peristaltic pump 21. However, in some other embodiments, when the perfusion module 2 adopts the manual perfusion mode, the main control module 3 is also used to control the rotation speed of the peristaltic pump 21 according to the currently set rotation speed, so that the perfusion pressure measured by the pressure detection module 22 can reach the target pressure. For example, there may be a certain relationship between the rotation speed preset by the main control module 3 and the target pressure. For example, when the main control module 3 controls the peristaltic pump 21 and the perfusion pressure measured by the pressure detection module 22 does not reach the target pressure, the staff can manually adjust the rotation speed of the peristaltic pump 21 until the perfusion pressure measured by the pressure detection module 22 reaches the target pressure.

[0034] In addition, it is worth mentioning that in order to enable the heating and storage module 1 to hold and heat the ex vivo animal organ, in some other embodiments, as Figure 3 shown, the heating and storage module 1 includes: an organ chamber 11, a heating module 12, and a temperature detection module 13. Among them, the organ chamber 11 is used to hold the ex vivo animal organ and the perfusion fluid, so that the ex vivo animal organ is immersed in the perfusion fluid. The heating module 12 can be used to heat the organ chamber 11. In addition, the temperature detection module 13 can be used to detect the temperature when the heating module 12 heats the organ chamber 11. Finally, combined with Figure 4 shown, the main control module 3 is respectively communicatively connected to the heating module 12 and the temperature detection module 13. The main control module 3 can be used to obtain the temperature measured by the temperature detection module 13, and compare the obtained temperature with the preset temperature. At the same time, the main control module 3 can also control the heating temperature of the heating module 12 according to the comparison result. For example, when the temperature measured by the temperature detection module 13 is greater than the preset temperature, it indicates that the heating temperature of the heating module 12 for the organ chamber 11 is too high. At this time, the main control module 3 can reduce the heating temperature when the heating module 12 heats the organ chamber 11. On the contrary, when the temperature measured by the temperature detection module 13 is less than the preset temperature, it indicates that the heating temperature of the heating module 12 for the organ chamber 11 is too low. At this time, the main control module 3 can increase the heating temperature when the heating module 12 heats the organ chamber 11.

[0035] Moreover, in order to enable the heating module 12 to heat the organ chamber 11, in some embodiments, as Figure 3 shown, the heating module 12 includes: a water bath chamber 121 and a heating assembly 122. Among them, the water bath chamber 121 can store a liquid medium and is detachably arranged in the organ chamber 11, while the heating assembly 122 is arranged at the bottom of the water bath chamber 121 and is communicatively connected to the main control module 3. At the same time, the heating assembly 122 is used to heat the water bath chamber 121, so that after the liquid medium stored in the water bath chamber 121 is heated, the heat can be conducted to the organ chamber 11. It is not difficult to find from this that since the organ chamber 11 is arranged in the water bath chamber 121, after the water bath chamber 121 is filled with a liquid medium, such as pure water, the organ chamber 11 can be partially immersed in the liquid medium in the water bath chamber 121. Thus, when the heating assembly 122 heats the water bath chamber 121, the heat can be evenly distributed throughout the water bath chamber 121 along with the liquid medium and conduct the heat to the organ chamber 11 to ensure that the temperature in the organ chamber 11 can always be kept at a constant temperature.

[0036] It should be noted that in order to enable the temperature detection module 13 to effectively detect the heating temperature when the heating assembly 122 heats the water bath chamber 121, in some embodiments, as Figure 3 shown, the temperature detection module 13 can adopt a temperature measuring probe, and the temperature measuring probe is used to at least partially insert into the water bath chamber 121 from the bottom of the water bath chamber 121, so that the temperature measuring probe can detect the temperature of the liquid medium stored in the water bath chamber 121 in real time.

[0037] In addition, in order to enable the heating assembly 122 to heat the water bath chamber 121, in some other embodiments, as Figure 3 shown, the heating assembly 122 includes: a heating tray 1221 and a heating sheet 1222. Among them, the heating tray 1221 is arranged at the bottom of the water bath chamber 121, and an installation groove 12211 is arranged on one side of the heating tray 1221 relative to the water bath chamber 121. In addition, as Figure 3 shown, the heating sheet 1222 is arranged in the installation groove 12211 of the heating tray 1221 and is in close contact with the bottom of the water bath chamber 121. At the same time, the heating tray 1221 is also provided with a wire groove 12212, and the wire 12221 of the heating sheet 1222 can be led out through the wire groove 12212, so that the wire 12221 can be electrically connected to the main control module 3 of the perfusion device, so that the main control module 3 can control the heating sheet 1222 to heat the water bath chamber 121. And, as a preferred solution, in some other embodiments, as Figure 3As shown, the heating assembly 122 further includes: a heat insulation sheet 1223, and the heat insulation sheet 1223 is disposed in the installation groove 12211 of the heating tray 1221, and the heat insulation sheet 1223 is located between the heating tray 1221 and the electric heating sheet 1222. The heat insulation sheet 1223 separates the heating tray 1221 from the electric heating sheet 1222, so that the heat generated when the electric heating sheet 1222 generates heat cannot be conducted to the heating tray 1221, making the surface of the heating tray 1221 have a lower temperature, thereby improving the safety of the heating and storage module 1 when heating an ex vivo animal organ. And, in some embodiments, in order to enable the heat insulation sheet 1223 to play a heat insulation role, the heat insulation sheet 1223 can be made of an asbestos gasket, and the asbestos gasket can effectively insulate the electric heating sheet 1222. Of course, in other embodiments, the heat insulation sheet 1223 can also be made of other materials, and in this embodiment, the material and type of the heat insulation sheet 1223 are not specifically limited.

[0038] And, it should be noted that, in some other embodiments, such as Figure 5 and Figure 6 As shown, in order to fix the heating and storage module on the outer shell 4 of the perfusion device, a positioning ring 20 can be additionally provided, and the positioning ring 20 can cooperate with the water bath chamber 121 to clamp the outer shell 4 of the perfusion device, so that the entire heating and storage module can be fixed on the outer shell 4. At the same time, it is worth noting that, in order to be able to fix the temperature measurement probe, a connection hole 201 for the temperature measurement probe to be inserted into the water bath chamber 121 is provided on the positioning ring 20, and the temperature measurement probe is locked and fixed on the positioning ring 20 with a locking member (such as a "nut"), so that the temperature measurement probe can stably detect the temperature of the liquid medium in the water bath chamber 121.

[0039] In addition, in some other embodiments, such as Figure 3 As shown, the organ chamber 11 includes: an organ chamber body 111 for containing an ex vivo animal organ and a perfusion fluid, and an organ chamber cover 112 for closing the organ chamber body 111. Among them, a plurality of first notches 1113 are provided along the circumferential direction of the organ chamber body 111, and a plurality of second notches 1122 are provided along the circumferential direction of the organ chamber cover 112, and the number of the first notches 1113 and the second notches 1122 is the same and they are uniquely corresponding, and each first notch 1113 communicates with the uniquely corresponding second notch 1122 for introducing a perfusion pipeline into the organ chamber body 111, so that the perfusion device can deliver the perfusion fluid to the ex vivo animal organ.

[0040] Specifically, in some embodiments, such as Figure 3As shown in the figure, the organ chamber body 111 includes: a funnel-shaped lower part 1111 and an annular upper part 1112. Among them, the funnel-shaped lower part 1111 has an upper edge 11111 and a lower edge 11112 opposite to the upper edge 11111, and the annular upper part 1112 is formed by protruding from the upper edge 11111 of the funnel-shaped lower part 1111 in a direction away from the lower edge 11112. Secondly, as Figure 3 shown, the organ chamber cover 112 is snap-connected to the upper edge 11111 of the funnel-shaped lower part 1111 and is snap-connected to the annular upper part 1112, and each first notch 1113 is provided on the annular upper part 1112. Through the funnel-shaped lower part 1111 of the organ chamber body 111, not only can the isolated animal organ be placed, but also the perfusion liquid can be received. At the same time, when the liquid medium in the water bath chamber 121 conducts heat to the organ chamber 11, the funnel-shaped lower part 1111 of the organ chamber body 111 can be immersed in the liquid medium to ensure that the temperature in the organ chamber 11 can always be kept at a constant temperature.

[0041] And, as a preferred solution, in some other embodiments, as Figure 3 shown, the heating and storage module 1 further includes: a filter screen assembly 14, and the filter screen assembly 14 is detachably arranged in the organ chamber body 111. The filter screen assembly 14 is used to support the isolated animal organ. At the same time, the position of the filter screen assembly 14 is adjustable along the height direction of the organ chamber body 111. It can be seen from this that by adjusting the height position of the filter screen assembly 14 hanging in the organ chamber body 111, the depth of the organ immersed in the perfusion liquid can be adjusted, so as to better maintain the temperature of the isolated animal organ and ensure the accuracy of the test data during the experiment.

[0042] And, it is worth noting that in order to enable the filter screen assembly 14 to support the isolated animal organ while also being able to adjust its height in the organ chamber body 111, as Figure 3 shown, the filter screen assembly 14 includes: a filter screen body (not marked in the figure) and a filter screen clamp 142. Among them, the filter screen body is used to support the isolated animal organ, and the filter screen clamp 142 has a head end 1421 and a tail end 1422 far from the head end 1421, and the filter screen clamp 142 winds around the filter screen body 142 from the head end 1421 to the tail end 1422 to support and fix the filter screen body 142. For example, in some embodiments, as Figure 3 shown, the filter screen clamp 142 can be an elastic component, and the filter screen clamp 142 is in an annular structure in the initial state, and the filter screen clamp 142 can be freely bent under the action of an external force, so that the filter screen clamp 142 can be elastically clamped to the filter screen body after surrounding the filter screen body. At the same time, corresponding to the head end 1421 and the tail end 1422 of the filter screen clamp 142, as Figure 3As shown, the filter screen assembly 14 further includes: a first lug 143 and a second lug 144. The first lug 143 is disposed at the head end 1421 of the filter screen clamp 142, and the second lug 144 is disposed at the tail end 1422 of the filter screen clamp 142. Moreover, the first lug 143 and the second lug 144 can also be used to move relative to each other under an external force, so that the filter screen clamp 142 collapses and is adjustable along the height direction of the organ chamber body 111. Conversely, the first lug 143 and the second lug 144 are also used to cause the filter screen clamp 142 to rebound and be fixedly clamped to the organ chamber body 111 after the external force is released.

[0043] In addition, during the long-term circulation of the perfusion fluid in the isolated animal organ, part of the perfusion fluid will evaporate under the influence of the heating temperature, resulting in a decrease in the liquid level of the perfusion fluid, and further causing changes in the temperature in the organ chamber, the biochemical indexes of the perfusion fluid, etc. Therefore, as a preferred solution, in some embodiments, as Figure 3 shown, the funnel-shaped lower part 1111 is further provided with a perfusion fluid level viewing window 1114, and the perfusion fluid level viewing window 1114 extends along the height direction of the organ chamber body 111. And, corresponding to the position of the perfusion fluid level viewing window 1114, as Figure 3 shown, the water bath chamber 121 is further provided with a water bath liquid level viewing window 123, and the water bath liquid level viewing window 123 also extends along the height direction of the water bath chamber 121, so that the perfusion fluid level viewing window 1114 and the water bath liquid level viewing window 123 can face each other. It is not difficult to see from this that through the perfusion fluid level viewing window 1114 and the water bath liquid level viewing window 123, the staff can observe the liquid level of the perfusion fluid in the funnel-shaped lower part 1111 at any time, thus not only ensuring that the perfusion fluid can circulate in the isolated animal organ in a sterile environment, but also observing the liquid level of the perfusion fluid through the perfusion fluid level viewing window 1114 and the water bath liquid level viewing window 123, so that the experimenter can timely supplement the perfusion fluid into the organ chamber body 111, and therefore ensuring that the perfusion fluid will not be drained dry due to evaporation at too high a temperature.

[0044] In addition, in some other embodiments, as Figure 1 and Figure 2 shown, the heating and storage module 1 further includes: a needle holder 15 and a bracket 17. Among them, the needle holder 15 is detachably disposed on the outer wall of the water bath chamber 121. At the same time, as Figure 2 shown, the needle holder 15 includes: a mounting portion 151 detachably connected to the outer wall of the water bath chamber 121, and a support portion 152 connected to the mounting portion 151. The support portion 152 is vertically extended along the height direction of the water bath chamber 121. A plurality of positioning holes 153 are further provided on the support portion 152, and the positioning holes 153 can be arranged along the extending direction of the support portion 152, so that the bracket 17 can be detachably disposed on the support portion 152 of the needle holder 15 through any one or more of the positioning holes 153. In addition, in some other embodiments, asFigure 1 and Figure 8 As shown, the heating and storage module 1 also includes: a pipeline clamp 16 and a gooseneck 18. Among them, one end of the gooseneck 18 is connected to the perfusion pipeline, and the other end is detachably fixed to the needle holder 15. The gooseneck 18 can be used to change the direction of the perfusion pipeline, thereby changing the delivery direction of the perfusion pipeline when delivering the perfusion liquid, so that the perfusion pipeline can better enter the organ bin body 111 through any first notch 1113 and the corresponding second notch 1122 to ensure that the perfusion liquid can circulate in the isolated animal organ. Secondly, the pipeline clamp 16 is used to clamp and fix one end of the gooseneck 18 connected to the perfusion pipeline to achieve clamping and fixing of the gooseneck 18. In addition, the bracket 17 can directly select the corresponding positioning hole 153 to be installed and fixed on the support portion 152 of the needle holder 15, and the bracket 17 can be used to install the pressure detection module 22, so that the pressure detection module 22 can achieve stable detection of the perfusion pressure when the perfusion liquid circulates in the isolated animal organ.

[0045] In addition, it is worth noting that in some embodiments, there are multiple perfusion modules 2 and heating and storage modules 1, and each perfusion module 2 and each heating and storage module 1 is connected to the main control module 3, so that the main control module 3 can simultaneously establish extracorporeal circulation for multiple isolated animal organs. Figure 1 As shown, the pouring device further comprises: a housing 4, and each pouring module 2 and each heating and receiving module 1 are detachably mounted on the housing 4, and multiple pouring modules 2 and multiple heating and receiving modules 1 can be integrated and mounted through the housing 4, thereby greatly reducing the occupied space of the whole machine. Specifically, in order to enable the housing 4 to realize the installation of each pouring module 2 and each heating and receiving module 1, in some embodiments, such as Figure 1 and Figure 9 As shown, a plurality of first slots 41 are provided on the shell 4, and a positioning ring 20 is provided on the side of the shell 4 away from the bottom of the first slot 41. Therefore, when fixing each heating and storage module 1, the water bath 121 of each heating and storage module 1 can cooperate with the positioning ring 20 to clamp the bottom of the first slot 41, and the water bath 121, the shell 4 and the positioning ring 20 are locked by screws 30, so that the entire heating and storage module 1 can be fixedly installed on the shell 4. Similarly, corresponding to the fixing method of the shell 4 to each heating and storage module 1, in other embodiments, such as Figure 1As shown, a plurality of second slots 42 for installing each perfusion module 2 can also be provided on the outer shell 4, and each perfusion module 2 can be installed in each second slot 42 respectively. At the same time, a plurality of screw holes (not marked in the figure) are provided along the periphery of each second slot 42. Therefore, when installing each perfusion module 2, the peristaltic pump 21 can be locked and fixed in the second slot by means of the cooperation between the bolt 23 and each screw hole in the second slot 42, so as to realize the installation and fixation of each perfusion module 2 on the outer shell 4.

[0046] Moreover, it should be noted that since each perfusion module 2 and each heating and storage module 1 can be integrally installed through the outer shell 4, in order to facilitate the effective monitoring of the working states of the perfusion module 2 and each heating and storage module 1, in some other embodiments, such as Figure 1 As shown, a display module 5 communicatively connected to the main control module 3 is also provided on the outer shell 4. The display module 5 can adopt a display screen or other display terminal devices. The display module 5 can be used to display the perfusion pressure data measured by the pressure detection module 22, as well as the temperature data measured by the temperature detection module 13, etc., so as to facilitate the staff to observe at any time and further improve the experimental efficiency.

[0047] Embodiment 2

[0048] Embodiment 2 of the present invention relates to a perfusion method for an isolated animal organ. As Figure 5 shown, the perfusion method is applied to the perfusion device as described in Embodiment 1, and the perfusion method includes the following steps:

[0049] Step 510, when providing power to the perfusion liquid in the heating and storage module 1 to make the perfusion liquid continuously circulate in the isolated animal organ, the main control module 3 obtains in real time the perfusion pressure when the perfusion liquid circulates in the isolated animal organ, and obtains in real time the temperature when the heating and storage module 1 heats the isolated animal organ.

[0050] Step 520, the main control module 3 controls the perfusion module 1 according to the obtained perfusion pressure, and controls the heating and storage module 1 according to the obtained temperature.

[0051] It is not difficult to see from the above that the heating and storage module 1 of the perfusion device can not only hold the ex vivo animal organ and the perfusion fluid, but also heat the ex vivo animal organ. At the same time, since the perfusion module 2 can also provide power to the perfusion fluid contained in the heating and storage module 1, enabling the perfusion fluid to continuously circulate through the ex vivo animal organ, an extracorporeal circulation can be established for the ex vivo animal organ, thus eliminating many potential contamination risk operations. In addition, since the main control module 3 can obtain the perfusion pressure in real time when the perfusion fluid circulates through the ex vivo animal organ, and obtain the temperature in real time when the heating and storage module 1 heats the ex vivo animal organ, and control the perfusion module according to the obtained perfusion pressure, and control the heating and storage module according to the obtained temperature, when establishing an extracorporeal circulation for the ex vivo animal organ, there is no need for experimenters to monitor parameters such as perfusion pressure and temperature, thus greatly saving manpower and improving experimental efficiency.

[0052] Specifically, in the step of the main control module 3 controlling the perfusion module 2 according to the obtained perfusion pressure, as Figure 6 shown, it specifically includes:

[0053] Step 52010, the main control module 3 compares the obtained perfusion pressure with the target pressure to determine whether to adjust the rotation speed of the peristaltic pump 21 of the perfusion module 2.

[0054] Step 52020, if the main control module 3 determines to adjust the rotation speed of the peristaltic pump 21, the main control module 3 increases or decreases the rotation speed of the peristaltic pump 21.

[0055] Step 52030, if the main control module 3 determines not to adjust the rotation speed of the peristaltic pump 21, the main control module 3 maintains the current rotation speed of the peristaltic pump 21.

[0056] It should be noted that in the step of the main control module 3 comparing the obtained perfusion pressure with the target pressure to determine whether to adjust the rotation speed of the peristaltic pump 21 of the perfusion module 2, that is, step 52010 specifically includes:

[0057] If the perfusion pressure obtained by the main control module 3 is equal to the target pressure, the main control module 3 determines not to adjust the rotation speed of the peristaltic pump 21.

[0058] If the perfusion pressure obtained by the main control module 3 is not equal to the target pressure, the main control module 3 determines to adjust the rotation speed of the peristaltic pump 21. And, in order to enable the main control module 3 to better adjust the rotation speed of the peristaltic pump 21, in the step of the main control module 3 increasing or decreasing the rotation speed of the peristaltic pump 21, that is, step 52020 specifically includes:

[0059] If the perfusion pressure obtained by the main control module 3 is greater than the target pressure, the main control module 3 decreases the rotation speed of the peristaltic pump 21.

[0060] If the perfusion pressure obtained by the main control module 3 is less than the target pressure, the main control module 3 increases the rotation speed of the peristaltic pump 21.

[0061] In addition, it is worth mentioning that in the step of the main control module 3 controlling the heating and storage module 1 according to the obtained temperature, as Figure 7 shown, it specifically includes:

[0062] Step 52040, the main control module 3 compares the obtained temperature with the preset temperature to determine whether to adjust the heating temperature of the heating and storage module 1.

[0063] Step 52050, if the main control module 3 determines to adjust the heating temperature of the heating and storage module 1, the main control module 3 increases or decreases the heating temperature of the heating and storage module 1.

[0064] Step 52060, if the main control module 3 determines not to adjust the heating temperature of the heating and storage module 1, the main control module 3 maintains the current heating temperature of the heating and storage module 1.

[0065] It should be noted that in the step of the main control module 3 comparing the obtained temperature with the preset temperature to determine whether to adjust the heating temperature of the heating and storage module 1, that is, step 52040 specifically includes:

[0066] If the temperature obtained by the main control module 3 is equal to the preset temperature, the main control module 3 determines not to adjust the heating temperature of the heating and storage module 1.

[0067] If the temperature obtained by the main control module 3 is not equal to the preset temperature, the main control module 3 determines to adjust the heating temperature of the heating and storage module 1. And, in order to enable the main control module 3 to better adjust the heating temperature of the heating and storage module 1, in the step of the main control module 3 increasing or decreasing the heating temperature of the heating and storage module 1, that is, step 52050 specifically includes:

[0068] If the temperature obtained by the main control module 3 is greater than the preset temperature, the main control module 3 decreases the heating temperature of the heating and storage module 1.

[0069] If the temperature obtained by the main control module 3 is less than the preset temperature, the main control module 3 increases the heating temperature of the heating and storage module 1.

[0070] It should be noted that this embodiment is an embodiment of the perfusion method corresponding to Embodiment 1, and this embodiment can be implemented in cooperation with Embodiment 1. The relevant technical details mentioned in Embodiment 1 are still valid in this embodiment. To avoid repetition, they are not elaborated here. Correspondingly, the relevant technical details mentioned in this embodiment can also be applied to Embodiment 1.

[0071] Those of ordinary skill in the art can understand that the above embodiments are specific embodiments for implementing the present invention, and in actual applications, various changes can be made to them in form and details without departing from the spirit and scope of the present invention.

Claims

1. A perfusion device for isolated animal organs, characterized in that: include: A heating and storage module, used for containing isolated animal organs and perfusion fluid, and for heating the contained isolated animal organs; A perfusion module, used to provide power to the perfusion liquid in the heating and storage module, so that the perfusion liquid continuously circulates in the isolated animal organ; A main control module, which is respectively connected to the heating and storage module and the perfusion module in communication; Wherein, the main control module is used to obtain the perfusion pressure of the perfusion fluid when it circulates in the isolated animal organ in real time, and control the perfusion module according to the obtained perfusion pressure; The main control module is also used to obtain in real time the temperature of the isolated animal organ when the heating and storage module heats it, and to control the heating and storage module according to the obtained temperature.

2. The perfusion device for isolated animal organs according to claim 1, characterized in that: The perfusion module comprises: A peristaltic pump, used to provide power to the perfusion liquid in the heating and storage module through the perfusion pipeline, so that the perfusion liquid continuously circulates in the isolated animal organ; A pressure detection module, used for detecting the perfusion pressure when the perfusion fluid circulates in the isolated animal organ; The main control module is respectively connected to the peristaltic pump and the pressure detection module for communication, and is used to obtain the perfusion pressure measured by the pressure detection module, and compare the obtained perfusion pressure with the perfusion pressure, and is also used to control the rotation speed of the peristaltic pump according to the comparison result; Alternatively, the main control module is used to control the rotation speed of the peristaltic pump according to the currently set rotation speed, so that the perfusion pressure measured by the pressure detection module reaches the target pressure.

3. The perfusion device for isolated animal organs according to claim 1, characterized in that: The heating and storage module comprises: The organ chamber is used to contain the isolated animal organs and the perfusion solution, so that the isolated animal organs are immersed in the perfusion solution; A heating module, used for heating the organ chamber; A temperature detection module, used for detecting the temperature of the organ chamber when the heating module heats the organ chamber; Among them, the main control module is communicated with the heating module and the temperature detection module respectively, and the main control module is used to obtain the temperature measured by the temperature detection module and compare the obtained temperature with the preset temperature. The main control module is also used to control the heating temperature of the heating module according to the comparison result.

4. The perfusion device for isolated animal organs according to claim 3, characterized in that: The heating module comprises: A water bath chamber, which can store liquid medium and is detachably disposed in the organ chamber; A heating component is arranged at the bottom of the water bath chamber and is communicatively connected with the main control module; the heating component is used to heat the water bath chamber so that the liquid medium stored in the water bath chamber transfers heat to the organ chamber after being heated; Wherein, the temperature detection module is a temperature measuring probe, and the temperature measuring probe is used to be at least partially inserted into the water bath chamber from the bottom of the water bath chamber, so that the temperature measuring probe can detect the temperature of the liquid medium stored in the water bath chamber in real time.

5. The perfusion device for isolated animal organs according to claim 4, characterized in that: The heating assembly comprises: A heating tray is arranged at the bottom of the water bath; wherein a mounting groove is arranged on one side of the heating tray relative to the water bath; The electric heater is arranged in the mounting groove of the heating tray and is tightly fitted with the bottom of the water bath; wherein the electric heater is electrically connected to the main control module through the connecting hole; The heat insulating sheet is arranged in the mounting groove of the heating tray and is located between the heating tray and the electric heating sheet to separate the heating tray from the electric heating sheet.

6. The perfusion device for isolated animal organs according to claim 4, characterized in that: The organ bin comprises: an organ bin body capable of containing isolated animal organs and perfusion fluid, and an organ bin cover for sealing the organ bin body; The organ bin body is provided with a plurality of first notches along its circumference, and the organ bin cover is provided with a plurality of second notches along its circumference, and the number of the first notches and the second notches are the same and uniquely correspond to each other, and each of the first notches is connected with the uniquely corresponding second notch for introducing the perfusion pipeline into the organ bin body.

7. The perfusion device for isolated animal organs according to claim 6, characterized in that: The organ bin body comprises: A bucket-shaped lower portion; the bucket-shaped lower portion has an upper edge and a lower edge opposite to the upper edge; An annular upper portion is formed by the upper edge of the bucket-shaped lower portion protruding in a direction away from the lower edge; Wherein, the organ compartment cover is buckled on the upper edge of the bucket-shaped lower part and is clamped with the annular upper part, and each of the first notches is arranged on the annular upper part.

8. The perfusion device for isolated animal organs according to claim 7, characterized in that: The heating and storage module also includes: The filter assembly is detachably arranged in the organ bin body and is used to support the isolated animal organ; wherein the position of the filter assembly along the height direction of the organ bin body is adjustable.

9. The perfusion device for isolated animal organs according to claim 8, characterized in that: The filter assembly comprises: The filter body is used to support the isolated animal organs; A filter clamp; the filter clamp has a head end and a tail end away from the head end, and the filter clamp is wrapped around the filter body from the head end to the tail end to support and fix the filter body; A first lug and a second lug; the first lug is arranged at the head end of the filter clamp, and the second lug is arranged at the tail end of the filter clamp; The first lug and the second lug are used to move relative to each other under the action of external force, so that the filter clamp collapses and is adjustable along the height direction of the organ bin body; the first lug and the second lug are also used to make the filter clamp rebound and be clamped and fixed to the organ bin body after the external force is released.

10. The perfusion device for isolated animal organs according to any one of claims 7 to 9, characterized in that: The lower part of the bucket is provided with a perfusion liquid level window, and the perfusion liquid level window extends along the height direction of the organ bin body; A water bath liquid level window is also provided on the water bath chamber, and the water bath liquid level window extends along the height direction of the water bath chamber.

11. The perfusion device for isolated animal organs according to claim 4, characterized in that: The heating and storage module also includes: The needle holder is detachably mounted on the outer wall of the water bath chamber; the needle holder comprises: a mounting portion detachably connected to the outer wall of the water bath chamber, and a supporting portion connected to the mounting portion; wherein the supporting portion is vertically extended in the height direction of the water bath chamber; A gooseneck tube; one end of the gooseneck tube is connected to the perfusion pipeline, and the other end is detachably fixed to the needle holder, and the gooseneck tube is used for reversing the perfusion pipeline; The pipeline clamp is used to clamp and fix one end of the gooseneck tube connected to the perfusion pipeline.

12. The perfusion device for isolated animal organs according to claim 1, characterized in that: There are a plurality of the perfusion modules and the heating and storage modules, and each of the perfusion modules and the heating and storage modules is communicatively connected to the main control module.

13. The perfusion device for isolated animal organs according to claim 1, characterized in that: The main control module is also used to record in real time the perfusion pressure data when the perfusion fluid circulates in the isolated animal organ, and to record the temperature data when the heating and storage module heats the isolated animal organ; The main control module is also used to export the recorded perfusion pressure data and the temperature data.

14. A method for perfusing an isolated animal organ, characterized in that: The perfusion method is applied to the perfusion device according to any one of claims 1 to 13, and the perfusion method comprises the following steps: When power is provided to the perfusion liquid in the heating and storage module so that the perfusion liquid continuously circulates in the isolated animal organ, the main control module obtains in real time the perfusion pressure of the perfusion liquid when it circulates in the isolated animal organ, and obtains in real time the temperature of the isolated animal organ when the heating and storage module heats the isolated animal organ; The main control module controls the perfusion module according to the obtained perfusion pressure, and controls the heating and storage module according to the obtained temperature.

15. The method for perfusing an isolated animal organ according to claim 14, characterized in that: The step in which the main control module controls the perfusion module according to the obtained perfusion pressure specifically includes: The main control module compares the obtained perfusion pressure with the target pressure to determine whether to adjust the rotation speed of the peristaltic pump of the perfusion module; If the main control module determines to adjust the rotation speed of the peristaltic pump, the main control module increases or decreases the rotation speed of the peristaltic pump; If the main control module determines not to adjust the rotation speed of the peristaltic pump, the main control module maintains the current rotation speed of the peristaltic pump.

16. The method for perfusing an isolated animal organ according to claim 15, characterized in that: The step in which the main control module compares the obtained perfusion pressure with the target pressure and determines whether to adjust the rotation speed of the peristaltic pump of the perfusion module specifically includes: If the perfusion pressure obtained by the main control module is equal to the target pressure, it is determined that the rotation speed of the peristaltic pump is not adjusted; If the perfusion pressure obtained by the main control module is not equal to the target pressure, it is determined that the rotation speed of the peristaltic pump is adjusted.

17. The method for perfusing an isolated animal organ according to claim 16, characterized in that: The step of increasing or decreasing the rotation speed of the peristaltic pump by the main control module specifically includes: If the perfusion pressure obtained by the main control module is greater than the target pressure, reducing the rotation speed of the peristaltic pump; If the perfusion pressure obtained by the main control module is less than the target pressure, the rotation speed of the peristaltic pump is increased.

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