Animal organ perfusion system and device

Abstract

The utility model belongs to the technical field of organ transplantation auxiliary equipment, and particularly relates to an animal organ perfusion system and equipment. The animal organ perfusion system comprises a temperature control pipeline loop and at least one perfusion pipeline loop. The perfusion pump, the filter, the oxygenator, the bubble remover, the flow sensor, the pressure sensor and the organ chamber are sequentially connected through the perfusion pipeline. The temperature control pipeline loop comprises a circulating liquid pipeline, and a temperature control device, a temperature sensor, a circulating pump and a bathtub which are sequentially connected through the circulating liquid pipeline. Wherein the bathtub is used for containing circulating liquid, the temperature control pipeline loop is used for regulating and controlling the temperature of the circulating liquid, and the organ chamber is arranged in the bathtub so as to be capable of exchanging heat with the circulating liquid. The animal organ perfusion equipment provided by the utility model is provided with the animal organ perfusion system so as to realize automatic perfusion of organs of small animals. The device is relatively miniaturized, the manufacturing cost is relatively low, and the cost of the small animal organ perfusion experiment is relatively low.

Description

Technical Field

[0001] The present application belongs to the technical field of organ transplant auxiliary equipment, and in particular relates to an animal organ perfusion system and equipment. Background Art

[0002] Currently, traditional organ preservation techniques primarily rely on low-temperature static cold storage, which does have a rather short shelf life. For example, the heart can be preserved for four hours, the liver for 12 hours, and the kidneys for approximately 24 hours. Digestive organs like the pancreas and small intestine are more challenging to preserve and have a shorter shelf life due to the presence of residual bacteria, digestive enzymes, and tissue-resident lymphocytes.

[0003] Mechanical perfusion (MP) technology is a novel method for organ preservation, transport, and evaluation. It continuously perfuses isolated organs with perfusion fluid during the preservation and transport phases, simultaneously supplying oxygen and nutrients to the organs, creating a physiologically similar environment. Mechanical perfusion not only prolongs organ preservation but also enables organ repair and in vitro evaluation, potentially expanding the source of liver donors.

[0004] However, the mechanical perfusion devices currently used in scientific research are mostly used for perfusion of large animal organs, which are large in size and have high experimental costs. Utility Model Content

[0005] The present application provides an animal organ perfusion system and equipment to solve the technical problem that existing large animal organ perfusion equipment is not suitable for perfusion of small animal organs.

[0006] According to one aspect of the present application, an animal organ perfusion system is provided, comprising a temperature control circuit and at least one perfusion circuit. The perfusion circuit comprises a perfusion circuit, and a perfusion pump, a filter, an oxygenator, a debubbler, a flow sensor, a pressure sensor, and an organ chamber, all connected in sequence via the perfusion circuit. The temperature control circuit comprises a circulating fluid circuit, and a temperature control device, a temperature sensor, a circulating pump, and a bathtub, all connected in sequence via the circulating fluid circuit.

[0007] The bathtub is used to hold the circulating fluid, the temperature control pipeline loop is used to regulate the temperature of the circulating fluid, and the organ chamber is arranged in the bathtub to be able to exchange heat with the circulating fluid.

[0008] In an optional solution of the present application, the organ chamber is used to hold the organ to be perfused and the perfusion fluid, and a bottom liquid outlet is provided at the bottom of the organ chamber; the liquid outlet end of the perfusion pump is connected to the organ to be perfused located in the organ chamber through the perfusion pipeline and via the filter, oxygenator, debubbler, flow sensor and pressure sensor; the liquid inlet end of the perfusion pump is connected to the bottom liquid outlet through the perfusion pipeline.

[0009] In an optional solution of the present application, a filter is used to remove organ metabolic waste and solid impurities in the perfusion fluid.

[0010] In an optional solution of the present application, the oxygenator is connected to an oxygen line to provide oxygen to the perfusate.

[0011] In an optional solution of the present application, a debubbler is used to remove bubbles in the perfusion pipeline loop.

[0012] In an optional solution of the present application, the bathtub is provided with a circulating liquid outlet and a circulating liquid inlet; the circulating liquid inlet is connected to the water outlet of the temperature control device through a circulating liquid pipeline via a circulating pump and a temperature sensor, and the circulating liquid outlet is connected to the water inlet of the temperature control device through a circulating liquid pipeline.

[0013] In an optional solution of the present application, the temperature control device allows the set temperature to be between 4°C and 37°C.

[0014] According to one aspect of the present application, an animal organ perfusion device is provided, comprising a housing, a power module, a control panel, a pipeline box, a display input device, a sampling module, and the above-mentioned animal organ perfusion system;

[0015] The display input device is disposed on the top side of the housing and is used to display information and input commands. The control board and the power module are disposed in the housing. The control board is connected to the display input device and the animal organ perfusion system to receive and process signals and control the operation of the device according to the signals. The power module is used to provide electrical energy.

[0016] The pipeline box is connected to the shell, and the sampling module is arranged in the bathtub for sampling and preservation during the perfusion process.

[0017] In an optional solution of the present application, a filter, an oxygenator, a debubbler, a flow sensor, a pressure sensor and part of the perfusion pipeline are integrated in the pipeline box.

[0018] In an optional solution of the present application, the sampling module includes a sampling tube bracket and a plurality of sampling tubes, and the plurality of sampling tubes are arranged on the sampling tube bracket.

[0019] In summary, the animal organ perfusion system and equipment provided by this application have at least the following beneficial effects:

[0020] In the animal organ perfusion system provided herein, the perfusion circuit is primarily used to perfuse the organ, and the temperature control circuit is primarily used to ensure the required temperature environment during the perfusion process. The animal organ perfusion equipment provided herein is equipped with the aforementioned animal organ perfusion system to enable automated perfusion of small animal organs.

[0021] Because the animal organ perfusion device is used for perfusing small animal organs, it is relatively small and has a relatively low manufacturing cost. Furthermore, small animal organ perfusion experiments are relatively inexpensive and more readily available, making it more conducive for researchers to conduct research on organ damage and repair. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] To more clearly illustrate the specific embodiments of this application or the technical solutions in the prior art, the following briefly introduces the drawings required for the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be derived from these drawings without inventive effort.

[0023] Figure 1 A schematic diagram of an animal organ perfusion device provided according to one embodiment of the present application;

[0024] Figure 2 This is a schematic diagram of an animal organ perfusion system provided according to one embodiment of the present application.

[0025] The reference numerals are as follows:

[0026] 1000. Animal organ perfusion system;

[0027] 100, perfusion circuit; 1, perfusion pump; 101, liquid outlet; 102, liquid inlet; 1A, first perfusion pump; 1B, second perfusion pump; 2, filter; 3, oxygenator; 4, perfusion circuit; 5, debubbler; 6, flow sensor; 7, pressure sensor; 8, organ to be perfused; 9, organ chamber; 9A, first organ chamber; 9B, second organ chamber; 901, bottom liquid outlet;

[0028] 200, temperature control circuit; 10, bathtub; 1001, circulating fluid outlet; 1002, circulating fluid inlet; 11, circulating pump; 12, temperature sensor; 13, temperature control device; 14, circulating fluid circuit; 15, circulating fluid;

[0029] 16. Control panel; 17. Pipeline box; 17A. First pipeline box; 17B. Second pipeline box; 18. Display input; 19. Power module; 20. Sampling module; 2001. Sampling tube holder; 2002. Sampling tube; 21. Housing;

[0030] 3000. Animal organ perfusion equipment. DETAILED DESCRIPTION

[0031] In the description of this application, it should be understood that if terms such as "upper", "lower", "top", "bottom", "inside", "outside" or the like appear to indicate orientation or positional relationships, unless otherwise specified, they are understood to be based on the orientation or positional relationships shown in the accompanying drawings. This is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on this application.

[0032] Furthermore, the use of "first" or "second" in describing features is for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features identified. Features identified as "first" or "second" may explicitly or implicitly include at least one of the identified features. The use of the word "plurality" generally implies at least two, such as two or three, unless otherwise specifically defined.

[0033] In this application, unless otherwise specified or limited, terms such as "mounted," "connected," "connect," and "fixed" should be interpreted broadly. For example, they can refer to fixed connections, removable connections, or integration; mechanical connections, electrical connections, direct connections, or indirect connections through an intermediary; and internal connections between two components or interactions between two components. Those skilled in the art will understand the specific meanings of these terms in this application based on the specific circumstances.

[0034] In the description of this specification, if the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" appear, it means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and the features of different embodiments or examples without contradiction.

[0035] Because current mechanical perfusion devices used in scientific research are primarily used for perfusing large animal organs, they are bulky and expensive, both in terms of cost and experimental costs. To address this, the present application provides an animal organ perfusion device 3000, primarily for perfusing small animal organs, achieving miniaturization. It should be understood that miniaturization reduces device costs, makes small animal organ perfusion experiments relatively inexpensive, and makes them more readily available, making them more conducive to research on organ damage and repair.

[0036] Figure 1 Schematic diagram of an animal organ perfusion device 3000 provided according to one embodiment of the present application. Figure 2 Schematic diagram of an animal organ perfusion system 1000 according to one embodiment of the present application. Figure 1 and Figure 2 The animal organ perfusion device 3000 includes a housing 21 , a power module 19 , a control panel 16 , a pipeline box 17 , a display input device 18 , a sampling module 20 and an animal organ perfusion system 1000 .

[0037] The animal organ perfusion system 1000 includes a temperature control circuit 200 and at least one perfusion circuit 100. The perfusion circuit 100 includes a perfusion circuit 4 and a perfusion pump 1, a filter 2, an oxygenator 3, a debubbler 5, a flow sensor 6, a pressure sensor 7, and an organ chamber 9, which are sequentially connected via the perfusion circuit 4.

[0038] The temperature control circuit 200 includes a circulating liquid pipeline 14 and a temperature control device 13 , a temperature sensor 12 , a circulating pump 11 and a bathtub 10 that are sequentially connected via the circulating liquid pipeline 14 .

[0039] The bathtub 10 is used to hold the circulating fluid 15 , the temperature control circuit 200 is used to control the temperature of the circulating fluid 15 , and the organ chamber 9 is disposed in the bathtub 10 to be able to exchange heat with the circulating fluid 15 .

[0040] Furthermore, a display input device 18 is located on the top side of the housing 21 and is used to display information and input commands. A control panel 16 and a power module 19 are located within the housing 21. The control panel 16 connects to the display input device 18 and the animal organ perfusion system 1000 to receive and process signals and control the operation of the equipment accordingly. The power module 19 provides electrical energy. A tubing box 17 is connected to the housing 21. A sampling module 20 is located within the tub 10 to collect and store samples during the perfusion process.

[0041] In this embodiment, the other components in the animal organ perfusion device 3000 are arranged based on the shell 21. Specifically, the pipeline box 17 integrates some components in the perfusion pipeline circuit 100, the remaining components in the perfusion pipeline circuit 100 can be integrated on the shell 21, and the components in the temperature control pipeline circuit 200 are mainly integrated on the shell 21.

[0042] In addition, the display input device 18 is fixedly mounted on the top side of the housing 21, allowing the experimenter to access its displayed information and input commands. The displayed information here primarily represents the operating status of the device, such as flow rate data and pressure data in the perfusion circuit 100, and temperature data in the temperature control circuit 200. Input commands here include, for example, start / stop commands for the perfusion pump 1 in the perfusion circuit 100 to control whether the perfusion pump 1 is turned on or off, and start / stop commands for the circulation pump 11 in the temperature control circuit 200 to control whether the circulation pump 11 is turned on or off.

[0043] In a specific application, control board 16 is connected to the perfusion pump 1, flow sensor 6, and pressure sensor 7 in the perfusion circuit 100, as well as the circulation pump 11 and temperature sensor 12 in the temperature control circuit 200. As can be seen, the signals received and processed by control board 16 primarily refer to these sensor signals and command signals input via display input 18, and the operation of the device is controlled based on these signals.

[0044] The power module 19 mainly provides power to the perfusion pump 1 , the flow sensor 6 , the pressure sensor 7 in the perfusion pipeline loop 100 and the circulation pump 11 and the temperature sensor 12 in the temperature control pipeline loop 200 .

[0045] For the animal organ perfusion system 1000 , the perfusion circuit 100 is mainly used to achieve organ perfusion, and the temperature control circuit 200 is mainly used to ensure the required temperature environment during the perfusion process.

[0046] Specifically, the temperature of the circulating fluid 15 is adjusted by the temperature control device 13 in the temperature control circuit 200. The temperature sensor 12 obtains the current temperature information of the circulating fluid 15. The control board 16 controls the temperature control device 13 based on the current temperature information, thereby maintaining the circulating fluid 15 in the bathtub 10 at the desired temperature. The circulation pump 11 is used to provide power to circulate the circulating fluid 15 in the temperature control circuit 200.

[0047] It should be understood that the organ chamber 9 located in the bathtub 10 can fully exchange heat with the circulating fluid 15, so that the temperature inside the organ chamber 9 is basically consistent with the temperature of the circulating fluid 15, so that the temperature inside the organ chamber 9 reaches the temperature required for perfusion.

[0048] In specific applications, the required temperature, flow rate, and pressure for perfusion can be set via display input 18. Control panel 16 controls perfusion pump 1, circulation pump 11, and temperature control device 13 based on information obtained from flow sensor 6, pressure sensor 7, and temperature sensor 12, ensuring that the perfusion process meets the set conditions. Circulating fluid 15 can be, for example, water or a liquid mixture containing antifreeze.

[0049] Based on the above, it can be seen that for the animal organ perfusion device 3000, in addition to integrating the animal organ perfusion system 1000 on the shell 21, the control board 16, the display input device 18, the power module 19, etc. are additionally integrated mainly to realize automated perfusion of animal organs.

[0050] It can be seen that the present application aims to provide a small automated animal organ perfusion device 3000 to achieve perfusion of small animal organs. The cost of small animal organ perfusion experiments is relatively low, and it is more convenient to obtain, which is more conducive to experimenters to conduct research on organ damage repair and other aspects.

[0051] In specific applications, the perfusion pump 1 and circulation pump 11 can be selected from any of the following types, such as peristaltic pumps and pulsed perfusion pumps, depending on the application requirements. The control board 16 is a PCB board on which a control module constructed as a controller is integrated, including, for example, a controller unit, a signal processing unit, a communication interface unit, and a memory. The display input 18 is a touch screen display. The power supply module 19 is a DC power supply module, which is used to convert AC power to DC power, such as 5V, 12V, or 24V, as required.

[0052] In an optional embodiment of the present application, an organ chamber 9 is used to hold an organ to be perfused 8 and perfusate. A bottom outlet 901 is provided at the bottom of the organ chamber 9. The liquid outlet 101 of the perfusion pump 1 is connected to the organ to be perfused 8 in the organ chamber 9 through the perfusion line 4, filter 2, oxygenator 3, debubbler 5, flow sensor 6, and pressure sensor 7. The liquid inlet 102 of the perfusion pump 1 is connected to the bottom outlet 901 through the perfusion line 4.

[0053] In this embodiment, both the organ to be perfused 8 and the perfusate are housed within an organ chamber 9. A bottom outlet 901 is provided at the bottom of the organ chamber 9, connected to the liquid inlet 102 of the perfusion pump 1. This outlet 901 ensures that all perfusate in the organ chamber 9 passes sequentially through the perfusion pump 1, filter 2, oxygenator 3, debubbler 5, and other components, ensuring the quality of the circulating perfusate. The perfusate then returns to the organ to be perfused 8 within the organ chamber 9, continuing the cycle.

[0054] In a specific application, the outer wall of the organ chamber 9 can fully contact with the circulating fluid 15 in the bathtub 10 to achieve the purpose of heat exchange.

[0055] Furthermore, the filter 2 is used to remove organ metabolic waste and solid impurities in the perfusate. The filter 2 is used to ensure the purity of the perfusate as much as possible. In specific applications, the filter can be, for example, a membrane filter, an adsorption filter, etc.

[0056] Furthermore, oxygenator 3 is connected to an oxygen line to provide oxygen to the perfusate. Depending on experimental requirements, oxygen can be introduced into oxygenator 3 to ensure the oxygen content of the perfusate. In specific applications, oxygenator 3 can be, for example, a membrane oxygenator, a hollow fiber membrane oxygenator, or a bubble oxygenator.

[0057] Furthermore, the debubbler 5 is used to remove bubbles from the perfusion circuit 100. The debubbler 5 removes bubbles from the perfusion circuit 100, preventing them from damaging the sample and improving experimental accuracy. It should be noted that the debubbler 5 contains a collection chamber. During the perfusion fluid circulation process, bubbles in the perfusion circuit 100 are expelled into the collection chamber as they pass through it.

[0058] In some optional embodiments, the bathtub 10 is provided with a circulating liquid outlet 1001 and a circulating liquid inlet 1002. The circulating liquid inlet 1002 is connected to the water outlet of the temperature control device 13 via the circulating liquid pipeline 14 via the circulating pump 11 and the temperature sensor 12, and the circulating liquid outlet 1001 is connected to the water inlet of the temperature control device 13 via the circulating liquid pipeline 14.

[0059] In this embodiment, the bathtub 10 has a circulating liquid outlet 1001 and a circulating liquid inlet 1002. The circulating liquid 15 in the bathtub 10, driven by the circulating pump 11, enters the temperature control device 13 through the circulating liquid outlet 1001, and then passes through the temperature sensor 12, the circulating pump 11 and the circulating liquid inlet 1002 in sequence and returns to the bathtub 10. This cycle is repeated to ensure that the circulating liquid 15 in the bathtub 10 is at the required temperature.

[0060] In this embodiment, the temperature control device 13 allows the temperature to be set between 4° C. and 37° C. That is, the temperature adjustment range is between 4° C. and 37° C. In a specific application, the temperature control device 13 is a semiconductor temperature control device that performs heating or cooling through semiconductors.

[0061] In some optional embodiments, the pipeline box 17 integrates the filter 2, the oxygenator 3, the debubbler 5, the flow sensor 6, the pressure sensor 7 and part of the perfusion pipeline 4.

[0062] In this embodiment, portions of the perfusion circuit 100 are integrated into a circuit box 17, achieving a modular layout. Specifically, the filter 2, oxygenator 3, debubbler 5, flow sensor 6, pressure sensor 7, and portions of the perfusion circuit 4 are integrated into the circuit box 17. Furthermore, the circuit box 17 is detachably connected to the housing 21, thus achieving a modular design for the device. The circuit box 17 can be used as a disposable consumable for easy replacement.

[0063] In a specific application, the pipeline box 17 can be detachably connected to the housing 21 by means of magnetic attraction, snap connection, screw connection, etc.

[0064] In some optional embodiments, the sampling module 20 includes a sampling tube holder 2001 and a plurality of sampling tubes 2002 , and the sampling tubes 2002 are disposed on the sampling tube holder 2001 .

[0065] In this embodiment, the sampling module 20 is composed of a sampling tube holder 2001 and a sampling tube 2002 . After sampling an organ in the organ chamber 9 , the organ can be placed in the corresponding sampling tube 2002 to complete the sampling.

[0066] exist Figure 1 In the illustrated embodiment, the housing 21 is a double-layer structure. For the temperature control circuit 200, except for the bathtub 10 disposed on the upper layer, the other components are integrated on the lower layer. The control board 16 and power module 19 are also integrated on the lower layer.

[0067] The perfusion circuit 100 is integrated into the upper layer, along with the sampling module 20 and display input device 18. There are two sets of perfusion circuits 100, with their corresponding components integrated into the first and second circuit boxes 17A, 17B, respectively. The first and second organ chambers 9A, 9B share a common tubing 10. The sampling module 20 is located between the two chambers. The first perfusion pump 1A connects the first circuit box 17A and the first organ chamber 9A, while the second perfusion pump 1A connects the second circuit box 17B and the second organ chamber 9B. The upper layer utilizes a symmetrical layout. Thus, the two perfusion circuits 100 share a single temperature-controlled circuit, ensuring a compact structure.

[0068] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Those skilled in the art can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. An animal organ perfusion system, characterized in that: include: At least one perfusion circuit (100), comprising a perfusion circuit (4) and a perfusion pump (1), a filter (2), an oxygenator (3), a debubbler (5), a flow sensor (6), a pressure sensor (7), and an organ chamber (9) sequentially connected via the perfusion circuit (4); and A temperature control pipeline loop (200) includes a circulating liquid pipeline (14) and a temperature control device (13), a temperature sensor (12), a circulating pump (11) and a bathtub (10) connected in sequence via the circulating liquid pipeline (14); The bathtub (10) is used to hold a circulating fluid (15), the temperature control circuit (200) is used to regulate the temperature of the circulating fluid (15), and the organ chamber (9) is arranged in the bathtub (10) so as to be able to perform heat exchange with the circulating fluid (15).

2. The animal organ perfusion system according to claim 1, characterized in that: The organ chamber (9) is used to hold the organ to be perfused (8) and the perfusion liquid, and the bottom of the organ chamber (9) is provided with a bottom liquid outlet (901); The liquid outlet (101) of the perfusion pump (1) is connected to the organ to be perfused (8) located in the organ chamber (9) through the perfusion pipeline (4) and via the filter (2), the oxygenator (3), the debubbler (5), the flow sensor (6) and the pressure sensor (7); The liquid inlet end (102) of the perfusion pump (1) is connected to the bottom liquid outlet (901) through the perfusion pipeline (4).

3. The animal organ perfusion system according to claim 2, characterized in that: The filter (2) is used to remove organ metabolic waste and solid impurities in the perfusion fluid.

4. The animal organ perfusion system according to claim 2, characterized in that: The oxygenator (3) is connected to an oxygen pipeline to provide oxygen to the perfusion fluid.

5. The animal organ perfusion system according to claim 1, characterized in that: The debubbler (5) is used to remove bubbles in the perfusion pipeline loop (100).

6. The animal organ perfusion system according to claim 1, characterized in that: The bathtub (10) is provided with a circulating liquid outlet (1001) and a circulating liquid inlet (1002); The circulating liquid inlet (1002) is connected to the water outlet of the temperature control device (13) through the circulating liquid pipeline (14) via the circulating pump (11) and the temperature sensor (12), and the circulating liquid outlet (1001) is connected to the water inlet of the temperature control device (13) through the circulating liquid pipeline (14).

7. The animal organ perfusion system according to claim 1, characterized in that: The temperature control device (13) allows the temperature to be set between 4°C and 37°C.

8. An animal organ perfusion device, characterized in that: The animal organ perfusion system (1000) comprises a housing (21), a power module (19), a control panel (16), a pipeline box (17), a display input device (18), a sampling module (20), and the animal organ perfusion system (1000) according to any one of claims 1 to 7; The display input device (18) is arranged on the top side of the housing (21) and is used to display information and input instructions. The control panel (16) and the power module (19) are arranged in the housing (21). The control panel (16) is connected to the display input device (18) and the animal organ perfusion system (1000) to receive and process signals and control the operation of the equipment according to the signals. The power module (19) is used to provide electrical energy. The pipeline box (17) is connected to the housing (21), and the sampling module (20) is arranged in the bathtub (10) for sampling and storage during the perfusion process.

9. The animal organ perfusion device according to claim 8, characterized in that The pipeline box (17) integrates the filter (2), the oxygenator (3), the debubbler (5), the flow sensor (6), the pressure sensor (7) and part of the perfusion pipeline (4).

10. The animal organ perfusion device according to claim 8 or 9, characterized in that: The sampling module (20) comprises a sampling tube support (2001) and a plurality of sampling tubes (2002), wherein the plurality of sampling tubes (2002) are arranged on the sampling tube support (2001).

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