The invention discloses a voice-activated myocardial protection perfusion system, comprising a box body, a box cover and a sterile inner container, wherein the box body is internally provided with a control box, a first perfusion bottle, a second perfusion bottle, a first perfusion pump and a second perfusion pump, the control box is internally provided with a first microcontroller, a first powersupply module, a specific person voice recognition circuit, an information recognition module connected with a sound collection module, a first flow sensor and a second flow sensor, an output end of the first microcontroller is connected with a first driving module, a second driving module, a first valve drive and a second valve drive, and the sterile inner container is provided with a first sprayhead and a second spray head. The sterile inner container of the system can be taken out from the box body to be disinfected and sterilized, and a medical aseptic standard is met; the effect of voice-controlled myocardial perfusion is realized by the specific human voice recognition circuit, the sound collection module and the information recognition module, and the stable and safe internal environment is ensured when the myocardium is transferred in vitro, thus reducing the disinfection frequency during transfer.