Tissue cultivating method of choerospondias axillaries
A technology of tissue culture and Zizyphus jujuba, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of difficult control of the number of male and female trees, low proportion of Zizyphus jujube, unfavorable cultivation of Zizyphus jujube, etc., and achieve biological preservation Characteristics, anti-browning, fast-growing effects
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[0021] Prepare the medium for each step:
[0022] Induction medium: Measure MS medium, add 1.5mg / L 6-BA, 0.03mg / L TDZ, 0.3mg / L IBA, 1% sucrose, and 2.0g / L agar in proportion to adjust the pH When the value is 6.0, the medium is sterilized at 121°C for 15 minutes;
[0023] Subculture medium: Measure MS medium, add 1.0mg / L 6-BA, 0.01mg / L TDZ, 0.4mg / L IBA, 0.02‰ activated carbon, 0.01‰ Vc, 2% Sucrose, 2.5g / L agar, adjust the pH value to 6.0, and sterilize the medium at 121°C for 15min;
[0024] Rooting medium: Measure 1 / 2MS medium, add 1.0mg / L 6-BA, 0.01mg / L TDZ, 0.6mg / L IBA, 0.5‰ activated carbon, 0.01‰ Vc, 3% sucrose, 2.5g / L agar, adjust the pH value to 6.0, and sterilize the medium at 121°C for 15min;
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