The invention provides a method for rapidly culturing
shoot tips
in vitro, which is characterized in that in the first step, selecting
stolon of the strawberries sprouted at the same year, washing to be clean and disinfecting; in the second step,
cutting 0.1-0.5 mm
shoot tips of the disinfected and cleaned strawberries
stolon under the anatomical lens, inoculating to a culture medium for
germination culture,
cutting the new sprouts after the 0.5-1.5 cm new sprouts are grown on the
shoot tips, inoculating to an enrichment medium for inducing clustered shoots; in the third step,
cutting the cluster buds to simple buds, inoculating to a rooting medium for rooting culture; transferring
test tube plantlets to a substrate formed by mixing
perlite and
peat, watering and covering a
plastic film for insulation and
moisture retention, revealing the
plastic film for gradually hardening, transferring the
seedling to a plastic potted tray filled with pure
peat, watering and covering the
plastic film for insulation and
moisture retention, and directly planting the
seedling to a field. The invention has the advantages of high reproductive rate, rapid
reproduction speed and
high survival rate.