Bacillus velezensis L1-21 and application thereof in preventing Huanglongbing

A technology of Bacillus velesi and Bacillus, applied in application, bacteria, chemicals for biological control, etc., can solve the problems of harmful wild hosts, large number of bacteria, only focus on prevention and control, etc., to achieve effective live bacteria The effect of high quantity, fast reproduction and stable performance

Inactive Publication Date: 2019-02-05
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the psyllid is infesting wild hosts in addition to citrus cultivated orchard plants
In the actual prevention and control process, only focusing on the prevention and control of psyllids in the orchard, it is impossible to completely eliminate all psyllids in the orchard, that is, it is difficult to completely cut off the way of psyllid transmission
Disease-free seedlings are exposed to psyllids for a long time after transplanting, and they will inevitably end up being damaged, and chemical pesticides still have a certain negative impact on the environment and ecology
Cutting down severely diseased plants reduces the source of the pathogen, but not cutting down potentially diseased plants that do not show symptoms of the disease keeps the pathogen in abundance in citrus orchards

Method used

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  • Bacillus velezensis L1-21 and application thereof in preventing Huanglongbing
  • Bacillus velezensis L1-21 and application thereof in preventing Huanglongbing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A strain of Bacillus Velez L1-21. The Bacillus Velez L1-21 is preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation number is: CGMCC NO.15726, and the classification is named: Bacillus Velez Bacillus velezensis L1-21.

[0024] A fermented liquid, the fermented liquid is Bacillus Velez L1-21 fermented liquid, made by fermentation of the above-mentioned Bacillus Velez L1-21.

[0025] A method for preparing a fermented liquid, the specific steps comprising:

[0026] Step 1: cultivating the Bacillus Velez L1-21 strain in a fermentation medium;

[0027] Step 2: The medium is sterilized at 121°C for 30 minutes, cooled to 37°C, and inoculated into the Erlenmeyer flask according to the amount of 1%-2% strain;

[0028] Step 3: Cultivate in medium at 37°C for 24 hours at a speed of 160 rpm, when the number of spores of Bacillus Velez L1-21 reaches 10 5 When the CFU / mL is reached, the fermentation is stopped t...

Embodiment 2

[0036] (1) Spraying and root irrigation of Bacillus Veles L1-21 fermented liquid on the foliage of citrus, the concentration of Bacillus Velez L1-21 bacterial strain for spraying and root irrigation is 10 10 CFU / mL of fermentation broth.

[0037] (2) Spray Bacillus Velez L1-21 water agent on the front and back sides of the leaves in the evening of non-rainy days, spray once every 7 to 10 days, and spray continuously for 6 months; dig around the trunk around the citrus drip line The ditch is 30cm wide x 25cm deep, and 5 kg of Bacillus Veles L1-21 bio-organic fertilizer is applied to each plant, mixed with soil, then filled into the ring ditch, and covered with soil.

[0038] (3) Dilute the Bacillus Velez L1-21 water solution by 1000 times to 10000 times, and spray it on the front and back sides of the leaves in the evening or before 8:00 am. Spray once and spray continuously for 6 months;

[0039] In this example, the variety is Wogan, the tree is 2 years old, and the control e...

Embodiment 3

[0044] (1) Spraying and root irrigation of Bacillus Veles L1-21 fermented liquid on the foliage of citrus, the concentration of Bacillus Velez L1-21 bacterial strain for spraying and root irrigation is 10 9 CFU / mL of fermentation broth.

[0045] (2) Spray Bacillus Velez L1-21 water agent on the front and back of the leaf surface in the evening on non-rainy days, spray once every 7 to 10 days, and spray continuously for 6 months; surround the trunk around the citrus drip line Dig a ditch with a width of 30cm x a depth of 25cm, apply 7 kg of Bacillus Veles L1-21 bio-organic fertilizer to each plant, mix it with soil, then fill it into the ring ditch and cover it with soil.

[0046] (3) Spray on the front and back sides of the leaves in the evening or before 8:00 am on a non-rainy day, and spray the Bacillus Veles L1-21 water agent every 7 to 10 days for 6 months continuously; In the example, the variety is Wogan, the age of the tree is 3 years, and the control effect is shown i...

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Abstract

The invention relates to the technical field of biological prevention and control, in particular to bacillus velezensis L1-21 and application thereof in preventing Huanglongbing. The bacillus velezensis L1-21 is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC NO.15726, and the category is named as bacillus velezensis L1-21. The bacillus velezensis L1-21 of the invention has the advantages of high stress resistance, simple nutrition, high reproduction speed, large effective live bacteria number, stable performance and long storage period. The bacillus velezensis L1-21 can prevent the plant disease due to the fact that the bacillus velezensis L1-21 can produce various antibacterial substances, which comprise various compoundssuch as lipopeptides, polyketides and the like, and the antibacterial substances can inhibit the normal growth of pathogenic fungus and bacteria, can kill the pathogenic fungus and bacteria and can play an effect of preventing and controlling the Huanglongbing.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a strain of Bacillus Velez L1-21 and its application for preventing and treating citrus huanglongbing. Background technique [0002] Citrus Huanglongbing (HLB) is a worldwide disease. Since it was first discovered in 1919, it has occurred in more than 40 countries and regions in the world. In addition to Sichuan, Chongqing and Hubei, other citrus producing areas in southern my country have occurred, and it is expanding and spreading. Its typical symptoms are mottled yellowing of leaves, defoliation, "red nose fruit", fruit drop, sharp drop in yield, smaller deformity of diseased fruit, soft and dull peel, sour taste, and reduced fruit quality, etc., which seriously affect the yield of citrus and quality, the fruit trees that have been onset for 5-6 years lose their commercial value, and even die and destroy the garden. Because of the difficulty in c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01N43/46A01P1/00C05G3/00A01G13/00C12R1/07
CPCA01G13/00A01N43/46A01N63/00A01N63/10C05D1/00C05G3/00C12N1/20C12N1/205C12R2001/07C05F5/002C05F11/08
Inventor 何月秋何鹏搏赵正龙亢波吴毅歆何鹏飞李兴玉李咏梅崔文艳何朋杰王娟
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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