19 results about "Polyketide" patented technology

Disclosed herein, inter alia, are methods of making polyketide compounds.

The present application belongs to the field of biotechnology, and particularly relates to a biosynthesis gene cluster of fungal polyketide compounds and application thereof. The present application provides a heterologous expression method of an aspertaichunol compound biosynthesis gene combination, and a compound aspertaichunol B is obtained through the method, and a compound aspertaichunol A is prepared through acid hydrolysis of the aspertaichunol B, which has important significance for future research and development of an efficient biosynthesis method for preparing the aspertaichunol compound.

The application relates to application of an ACADM gene in improving the production of spinosyn in Saccharopolyspora spinosa, and belongs to the field of biological fermentation. The method mainly comprises overexpressing the ACADM gene in the Saccharopolyspora spinosa. Specifically, by introducing the ACADM gene into the Saccharopolyspora spinosa and enabling the ACADM gene to realize efficient expression in the host bacteria, the biosynthesis pathway of the spinosyn can be effectively promoted, so that the final yield of the spinosyn is significantly improved. Experiments show that the method is feasible in operation and remarkable in effect, and provides an effective genetic modification strategy for industrial production of the spinosyn.

NEW SMALL MOLECULES AND METHODS USING THEM FOR SKIN TREATMENT. The disclosure relates to new biosynthetic gene clusters encoding type II polyketides, their products, extracts and compositions comprising the products, and methods for using the products, extracts, and compositions for skin treatment, e.g., to enhance the skin's barrier function and / or immune defenses. Figure for abstract: none

The invention belongs to the technical field of genetic engineering and biosynthesis, and particularly relates to a streptomyces-sourced siderophore-polyketone hybrid product synthesis related gene cluster, a genetically engineered bacterium and application of the genetically engineered bacterium. The invention provides an NIS siderophore synthetase gene cluster. The gene cluster is selected from a gene cluster BGC19 or a gene cluster desB. Meanwhile, the invention discloses two biological heterologous or ectopic expression engineering bacteria for producing the hybrid siderophore and application of the siderophore-polyketone hybrid compound by taking streptomyces as a host, and discloses structures and activities of the siderophore-polyketone hybrid compound produced by the two engineering bacteria. The invention provides a scheme for the construction of engineering bacteria for generating new hybrid compounds by utilizing the expression of non-adjacent gene clusters at specific sites and functional combination among the gene clusters, also provides a new element for the biosynthesis of a novel siderophore and polyketone combination, and has good practical application value.

The invention belongs to the technical field of medical chemistry, and particularly relates to a polycyclic polyketone derivative as well as a preparation method and application thereof in treating hepatic fibrosis. Six polycyclic polyketone compounds are obtained from a fungus M. arundinis HL1 separated from a marine sample in Huilaxian County, Guangdong Province, the series of compounds can significantly inhibit expression of FN, Collagen I, alpha-SMA and other fibrosis-related factors in hepatic stellate cells LX-2, and the compound 2 has a significant inhibition effect at a concentration of 40 [mu] mol / L and has no significant toxicity. And the compound 2 reduces the p-Smad2 / 3 level through dose dependence, does not influence the total Smad2 / 3 level, and plays an anti-fibrosis role by inhibiting a TGF-beta / Smad signal channel. The series of compounds, especially the compound 2, are expected to become anti-hepatic fibrosis candidate drugs, and provide a brand new chemical skeleton for research and development of low-toxicity and high-efficiency anti-hepatic fibrosis drugs.

The present application relates to a kind of polyketide alkaloid hybrid compound and its preparation method and application, wherein, the polyketide alkaloid hybrid compound is selected from at least one of the compounds with the structural formula shown in formula I.The compound 1~6 structure includes an atypical angular anthracene ring polyketide ring and open-loop ternary nitrogen ring, both are novel compounds of skeleton.The polyketide alkaloid hybrid compound of the present application has the activity of inhibiting neuraminidase and influenza virus H1N1 and H3N2, and can be used for preparing anti-influenza virus drugs;Meanwhile, it also has the activity of resisting bacteria and actinomycetes, and can be used for preparing antibacterial agent, with good application prospect.

The invention belongs to the field of marine natural product chemistry, and particularly relates to a mangrove-derived fungus heterocyclic dipeptide compound and application thereof. According to the invention, a secondary metabolite of mangrove-derived fungus XLN32122 is researched, and a new heterocyclic dipeptide compound 1 containing 1, 2-oxa-dodecanoic ring, three analogues (2-4) and three known polyketone compounds (5-7) are separated from the secondary metabolite. An in-vitro anti-inflammatory activity result shows that the compound 3 has remarkable inhibitory activity on the generation of NO in RAW 264.7 cells induced by LPS, the IC50 value of the compound 3 is 13.13 + / -0.005 mu M and is higher than that of a positive control drug dexamethasone, and the IC50 value of the compound 3 is 136.84 + / -1.330 mu M.

This invention relates to a novel natural microbial metabolite: Fcl-8, derived from the fermentation broth of *Xenorhabdus budapestensis* XBD8. Purified Fcl-8 is a snow-white, flocculent crystal, highly soluble in water, as shown in Figure 1. The chemical structural formula of Fcl-8 is shown in Figure 2, with the chemical formula C1. 57 H 102 N 14 O 12 With a molecular weight of 1175.78744, its typical secondary mass spectrometry information is shown in Figure 3. Fcl-8 is derived from a natural microbial metabolite, has a novel structure and high biological activity, and possesses great potential for development into a novel biopesticide.

This invention relates to the field of biomedical technology, and discloses an aromatic polyketide compound, its preparation method, and its applications. The aromatic polyketide compound is derived from... Crystallomyces hypericus Compound I and Compound II were isolated from the ethyl acetate extract of rice fermentation broth and identified as Crystalloketide A and Crystallomycin D, respectively. In vitro anticancer activity tests were conducted on Crystalloketide A and Crystallomycin D. The results showed that Compound I and Compound II inhibited human cervical cancer cells, human liver cancer cells, human neurocancer cells, and human pancreatic cancer cells, respectively. Therefore, it is further suggested that Compound I and Compound II described in this invention can be used to prepare anticancer drugs.

The application belongs to the technical field of biological medicine, and particularly relates to a preparation method and application of a polyketide compound from a fungus of the genus Arenarius. The polyketide compound can dose-dependently reduce the NO generation level in cells, significantly inhibit M1 phenotype polarization and promote the conversion of the M1 phenotype to the M2 phenotype, significantly down-regulate the expression of key inflammatory-related proteins (including IL-1beta, IL-6, TNF-alpha and COX-2) in cells, and inhibit the generation of NLRP3 inflammasome and caspase-1, revealing the potential anti-AD activity and indicating that the polyketide compound has a good application prospect in the preparation of anti-neuroinflammatory drugs. In addition, the polyketide compound can be prepared from a marine microorganism Arenarius fungus, and the preparation method is simple and the source is rich.

A genetically modified microorganism for increasing the production of a polyketide compound or a derivative thereof is provided, including any one or both of the following genetic modifications: (a) a deleted endogenous pta gene encoding a phosphate acetyltransferase, wherein an expression level of the phosphate acetyltransferase of the deleted endogenous pta gene is lower than an expression level of its wild type; and (b) an added first exogenous nucleotide sequence and second exogenous nucleotide sequence, wherein the first exogenous nucleotide sequence encodes a transaminase and the second exogenous nucleotide sequence encodes a reductase. A method for producing a polyketide compound or a derivative thereof using the aforementioned genetically modified microorganism is also provided.

The invention discloses an all-carbon macrocyclic polyketone compound Akaeolide as well as a biosynthetic gene cluster and application of the all-carbon macrocyclic polyketone compound Akaeolide. The biosynthetic gene cluster of AKA comprises eight genes. All AKA-containing biosynthesis-related genes, protein information and specific functions of each protein provided by the invention can help people to understand the biosynthesis mechanism of all-carbon macrocyclic polyketone natural products. In addition, heterologous expression and engineering modification of the molecules are realized by utilizing BAC plasmids, and materials and knowledge are provided for further genetic modification. The gene and the protein provided by the invention can provide targets for subsequent gene mining.

The invention discloses a polyamide composite material as well as a preparation method and application thereof. The polyamide composite material comprises the following components in parts by weight: 50-100 parts of polyamide resin; 30 to 50 parts of polyketone resin; 30 to 60 parts of glass fiber; 6 to 12 parts of an ionomer; 2 to 6 parts of compatilizer; and the compatilizer is selected from at least one of ethylene-butyl acrylate-glycidyl methacrylate or ethylene-methyl acrylate-glycidyl methacrylate. In a glass fiber reinforced polyamide / polyketone material system, the specific ionomer and the compatilizer are added, so that the polyamide composite material with high rigidity, low-temperature toughness and excellent wear resistance is realized, and the application requirements on the material in special scenes such as sports equipment, industrial building structural members or low-altitude economic equipment can be met.

A process for the preparation of anti-Malassezia powder.The invention relates to a process the preparation of anti Malassezia mixture from bacillus species. it particularly relates to the production of antipeptide and polyketides from bacillus species using inexpensive fermentation medium. More particularly it relates to the use of macrolactin in anti Malassezia mixture.

The application belongs to the technical field of medical compounds, and particularly relates to an anti-tumor polyketide-nonribosomal peptide hybrid compound, a preparation method and application. The polyketide-nonribosomal peptide hybrid compound is compound 1 or compound 2, and structural formulas of the compound 1 and the compound 2 are as follows: The compound has anti-tumor activity, particularly anti-human breast cancer effect, and has relatively high anti-tumor activity.

The invention relates to the technical field of natural medicines, in particular to a semi-cage-shaped sorbitol polyketone compound as well as a preparation method and application thereof. The method comprises the following steps: by taking a rice secondary metabolite of trichoderma reesei fungus as a raw material, soaking, extracting and concentrating to obtain an extract, suspending the extract in water to obtain a suspension, and extracting and concentrating to obtain an ethyl acetate part extract; the extract is taken and subjected to separation through a positive and negative phase silica gel column, MCI medium-pressure column chromatography, Sephadex LH-20 gel column chromatography and high performance liquid chromatography purification, and the semi-cage-shaped sorbitol polyketone compound is prepared. The compounds show significant potential in the aspect of being developed into innovative drugs for preventing and treating calcified aortic valve diseases, and the unique molecular structure of the compounds opens up an original design path for research and development of novel drugs for the diseases.

The application discloses an aromatic polyketone compound and a preparation method and application thereof, and belongs to the field of biological medicines. Two aromatic polyketone compounds are produced by streptococcus, and the structural formula is shown as formula (I). The antibacterial activity test is carried out on the compounds 1 and 2, the compounds 1 and 2 have broad-spectrum antibacterial activity on four kinds of gram-positive bacteria, and therefore the aromatic polyketone compounds can provide alternative compounds for developing new antibacterial drugs.