46 results about "Polyketide" patented technology

The invention relates to a brand-new microbial natural metabolite, namely Fcl-8, which is derived from a fermentation solution of Xenorhabdius budapestensis XBD8, and is characterized in that the Fcl-8 is a new microbial natural metabolite, namely Fcl-8, which is derived from the fermentation solution of the Xenorhabdius budapestensis XBD8. The purified Fcl-8 is a snow white flocculent crystal and is very easy to dissolve in water, and the solid state of the purified Fcl-8 is as shown in Figure 1. The chemical structural formula of the Fcl-8 is as shown in a figure 2, the chemical formula is C57H102N14O12, the molecular weight is 1175.78744, and the typical secondary mass spectrum information of the Fcl-8 is as shown in a figure 3. The Fcl-8 is derived from a microbial natural metabolite, is novel in structure and high in biological activity, and has very high potential to be developed into a novel biopesticide.

This invention relates to a combined module for increasing the yield of polyketide natural products from actinomycetes, and the construction and application of recombinant bacteria containing this module, belonging to the field of genetic engineering technology. To improve the yield of natural products such as polyketides and nonribosomal peptides, this invention utilizes a metabolic engineering strategy to combine and modify the CoASH synthetic pathway and the PPTase post-modification pathway, providing a combined module that can increase the yield of natural products from actinomycetes. This module consists of the CoASH cofactor synthesis module element SCoaA. R106A Composed of SCoaD and PPTase post-modification module element HPC3, recombinant vectors and recombinant bacteria containing this module were constructed. It was found that overexpression of this module can effectively increase the yield of natural products such as actinomycete polyketides, polyethers, and non-ribosomal peptides.

The present invention relates to the application of phosphopantetheinyltransferase E7X1, its encoding gene, recombinant vectors containing the gene, and recombinant bacteria, belonging to the field of genetic engineering technology. To increase the yield of natural products such as polyketides and non-ribosomal peptides, the present invention utilizes bioinformatics analysis and other means to mine a highly efficient phosphopantetheinyltransferase E7X1 from a database. The amino acid sequence of the enzyme is shown in SEQ ID NO.1, and the nucleotide sequence of the gene encoding the enzyme is shown in SEQ ID NO.2. By constructing a recombinant vector and recombinant bacteria containing the enzyme encoding gene, the present invention discovered that overexpressing the enzyme can effectively increase the yield of natural products such as polyketides, polyethers, and non-ribosomal peptides.

Disclosed herein, inter alia, are methods of making polyketide compounds.

The application relates to the technical field of synthetic chemistry, and provides a preparation method of alpha-pyrone-polyketone, which comprises the following steps: coupling reaction of compound 3 and compound 2 to obtain compound 26; secondary alcohol group of the compound 26 is removed to obtain compound 27; the compound 27 is removed from a protection group to obtain the alpha-pyrone-polyketone shown in compound 1; wherein the structural formula of the compound is as follows: the preparation method of the alpha-pyrone-polyketone provided in the application is based on the instability of the bis-bisolefin, and is constructed by using an alkenyl tin intermediate and an alkenyl iodine intermediate through a coupling reaction, so that the operation is simple, the yield of each step is high, and the method can be widely popularized and applied.

The present application belongs to the field of biotechnology, and particularly relates to a biosynthesis gene cluster of fungal polyketide compounds and application thereof. The present application provides a heterologous expression method of an aspertaichunol compound biosynthesis gene combination, and a compound aspertaichunol B is obtained through the method, and a compound aspertaichunol A is prepared through acid hydrolysis of the aspertaichunol B, which has important significance for future research and development of an efficient biosynthesis method for preparing the aspertaichunol compound.

The invention discloses a naphthocycline type pentacyclic aromatic polyketone compound. The structural formula of the naphthocycline type pentacyclic aromatic polyketone compound is as shown in a formula (I): # imgabs0 #. According to the invention, six naphthocycline type pentacyclic aromatic polyketone compounds are separated from a rice fermentation product of actinomycetes Streptomyces sp.NAK5495 for the first time, and the six naphthocycline type pentacyclic aromatic polyketone compounds are separated from rice fermentation products of actinomycetes Streptomyces sp.NAK5495; according to the present invention, with the medium-pressure liquid chromatography (MPLC) system and the semi-preparative HPLC separation and purification technology, the efficient separation and purification of the naphthocycline pentacyclic aromatic polyketones are achieved, and the recovery rate and the purity of the target compound are improved.

The application relates to application of an ACADM gene in improving the production of spinosyn in Saccharopolyspora spinosa, and belongs to the field of biological fermentation. The method mainly comprises overexpressing the ACADM gene in the Saccharopolyspora spinosa. Specifically, by introducing the ACADM gene into the Saccharopolyspora spinosa and enabling the ACADM gene to realize efficient expression in the host bacteria, the biosynthesis pathway of the spinosyn can be effectively promoted, so that the final yield of the spinosyn is significantly improved. Experiments show that the method is feasible in operation and remarkable in effect, and provides an effective genetic modification strategy for industrial production of the spinosyn.

NEW SMALL MOLECULES AND METHODS USING THEM FOR SKIN TREATMENT. The disclosure relates to new biosynthetic gene clusters encoding type II polyketides, their products, extracts and compositions comprising the products, and methods for using the products, extracts, and compositions for skin treatment, e.g., to enhance the skin's barrier function and / or immune defenses. Figure for abstract: none

The invention belongs to the technical field of genetic engineering and biosynthesis, and particularly relates to a streptomyces-sourced siderophore-polyketone hybrid product synthesis related gene cluster, a genetically engineered bacterium and application of the genetically engineered bacterium. The invention provides an NIS siderophore synthetase gene cluster. The gene cluster is selected from a gene cluster BGC19 or a gene cluster desB. Meanwhile, the invention discloses two biological heterologous or ectopic expression engineering bacteria for producing the hybrid siderophore and application of the siderophore-polyketone hybrid compound by taking streptomyces as a host, and discloses structures and activities of the siderophore-polyketone hybrid compound produced by the two engineering bacteria. The invention provides a scheme for the construction of engineering bacteria for generating new hybrid compounds by utilizing the expression of non-adjacent gene clusters at specific sites and functional combination among the gene clusters, also provides a new element for the biosynthesis of a novel siderophore and polyketone combination, and has good practical application value.

The present invention relates to the application of phosphopantetheinyltransferase A5L6, its encoding gene, recombinant vectors containing the gene, and recombinant bacteria, belonging to the field of genetic engineering technology. To increase the yield of natural products such as polyketides and non-ribosomal peptides, the present invention utilizes bioinformatics analysis and other means to mine a highly efficient phosphopantetheinyltransferase A5L6 from a database. The amino acid sequence of the enzyme is shown in SEQ ID NO.1, and the nucleotide sequence of the gene encoding the enzyme is shown in SEQ ID NO.2. By constructing a recombinant vector and recombinant bacteria containing the enzyme encoding gene, the present invention discovered that overexpressing the enzyme can effectively increase the yield of natural products such as polyketides, polyethers, and non-ribosomal peptides.

The present invention relates to the application of phosphopantetheinyltransferase AU23, its encoding gene, recombinant vectors containing the gene, and recombinant bacteria, which belong to the field of genetic engineering technology. To increase the yield of natural products such as polyketides and non-ribosomal peptides, the present invention uses bioinformatics analysis and other means to mine a highly efficient phosphopantetheinyltransferase AU23 from a database. The amino acid sequence of the enzyme is shown in SEQ ID NO.1, and the nucleotide sequence of the gene encoding the enzyme is shown in SEQ ID NO.2. By constructing a recombinant vector and recombinant bacteria containing the enzyme encoding gene, the present invention found that overexpressing the enzyme can effectively increase the yield of natural products such as polyketides, polyethers, and non-ribosomal peptides.

The invention belongs to the technical field of medical chemistry, and particularly relates to a polycyclic polyketone derivative as well as a preparation method and application thereof in treating hepatic fibrosis. Six polycyclic polyketone compounds are obtained from a fungus M. arundinis HL1 separated from a marine sample in Huilaxian County, Guangdong Province, the series of compounds can significantly inhibit expression of FN, Collagen I, alpha-SMA and other fibrosis-related factors in hepatic stellate cells LX-2, and the compound 2 has a significant inhibition effect at a concentration of 40 [mu] mol / L and has no significant toxicity. And the compound 2 reduces the p-Smad2 / 3 level through dose dependence, does not influence the total Smad2 / 3 level, and plays an anti-fibrosis role by inhibiting a TGF-beta / Smad signal channel. The series of compounds, especially the compound 2, are expected to become anti-hepatic fibrosis candidate drugs, and provide a brand new chemical skeleton for research and development of low-toxicity and high-efficiency anti-hepatic fibrosis drugs.

Provided herein are synthetic methods and intermediates useful in the preparation of polyketides, including 12-membered ring macrolides. These synthetic methods and intermediates provide scalable access to polyketides, including polyketides with potent bioactive splicing modulator activity and therapeutic potential, including treating various types of cancer. The polyketides include 12-membered ring macrolides, including Compound A, Compound B, and pladienolides, including pladienolide A, pladienolide B, pladienolide C, pladienolide D, pladienolide E, pladienolide F, and pladienolide G.

The present application relates to a kind of polyketide alkaloid hybrid compound and its preparation method and application, wherein, the polyketide alkaloid hybrid compound is selected from at least one of the compounds with the structural formula shown in formula I.The compound 1~6 structure includes an atypical angular anthracene ring polyketide ring and open-loop ternary nitrogen ring, both are novel compounds of skeleton.The polyketide alkaloid hybrid compound of the present application has the activity of inhibiting neuraminidase and influenza virus H1N1 and H3N2, and can be used for preparing anti-influenza virus drugs;Meanwhile, it also has the activity of resisting bacteria and actinomycetes, and can be used for preparing antibacterial agent, with good application prospect.

The invention belongs to the field of marine natural product chemistry, and particularly relates to a mangrove-derived fungus heterocyclic dipeptide compound and application thereof. According to the invention, a secondary metabolite of mangrove-derived fungus XLN32122 is researched, and a new heterocyclic dipeptide compound 1 containing 1, 2-oxa-dodecanoic ring, three analogues (2-4) and three known polyketone compounds (5-7) are separated from the secondary metabolite. An in-vitro anti-inflammatory activity result shows that the compound 3 has remarkable inhibitory activity on the generation of NO in RAW 264.7 cells induced by LPS, the IC50 value of the compound 3 is 13.13 + / -0.005 mu M and is higher than that of a positive control drug dexamethasone, and the IC50 value of the compound 3 is 136.84 + / -1.330 mu M.

The invention belongs to the field of microbial natural product pesticides, and particularly discloses a polyketone compound, a preparation method and application in prevention and treatment of cucumber fusarium wilt. The compound disclosed by the invention is prepared by separating from a liquid fermentation product of Aspergillus japonicus TE-739D, and the compound disclosed by the invention is prepared from the liquid fermentation product of Aspergillus japonicus TE-739D. The chemical structure of the compound is identified through a modern spectroscopy technology, such as high resolution mass spectrum HRESIMS and superconductive nuclear magnetic resonance spectrum NMR, and the compound is named as Japonidiene H. The polyketone compound Japonidiene H can be used for remarkably inhibiting the growth of fusarium oxysporum of cucumbers.

The present invention relates to the application of phosphopantetheinyltransferase B3D0, its encoding gene, recombinant vectors containing the gene, and recombinant bacteria, belonging to the field of genetic engineering technology. To increase the yield of natural products such as polyketides and non-ribosomal peptides, the present invention utilizes bioinformatics analysis and other means to mine a highly efficient phosphopantetheinyltransferase B3D0 from a database. The amino acid sequence of the enzyme is shown in SEQ ID NO.1, and the nucleotide sequence of the gene encoding the enzyme is shown in SEQ ID NO.2. By constructing a recombinant vector and recombinant bacteria containing the enzyme encoding gene, the present invention discovered that overexpressing the enzyme can effectively increase the yield of natural products such as polyketides, polyethers, and non-ribosomal peptides.

Provided herein are synthetic methods and intermediates useful in the preparation of polyacetyl compounds. And the polyacetyl compound comprises a 12-membered ring macrolide. These synthetic methods and intermediates provide a scalable pathway to obtain polyacetyl compounds, including polyacetyl compounds with potent bioactive splicing modulator activity and potential for therapeutic applications, including the treatment of different types of cancer.

Disclosed herein are compositions and methods of use thereof. For example, described herein are engineered 2-pyrone synthases, methods of use thereof for producing polyketides, polyketide precursors, polyketide derivatives, or a combination thereof, and compositions comprising the same.

Disclosed herein are recombinant methods of activating expression of one or more biosynthetic gene clusters comprising more than one gene, the method comprising a recombinant DNA expression vector that possess two opposable inducible promoters that drives expression of a biosynthetic gene cluster exogenously from outside of the cluster to produce polyketides or non-ribosomal peptides in a heterologous host.

This invention relates to a novel natural microbial metabolite: Fcl-8, derived from the fermentation broth of *Xenorhabdus budapestensis* XBD8. Purified Fcl-8 is a snow-white, flocculent crystal, highly soluble in water, as shown in Figure 1. The chemical structural formula of Fcl-8 is shown in Figure 2, with the chemical formula C1. 57 H 102 N 14 O 12 With a molecular weight of 1175.78744, its typical secondary mass spectrometry information is shown in Figure 3. Fcl-8 is derived from a natural microbial metabolite, has a novel structure and high biological activity, and possesses great potential for development into a novel biopesticide.

This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyloin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds. These condensation reactions serve as a platform for the synthesis of isoprenoid precursors when utilized in combination with a variety of metabolic pathways and enzymes for carbon rearrangement and the addition / removal of functional groups. Isoprenoid alcohols are key intermediary products for the production of isoprenoid precursors in these novel synthetic metabolic pathways. These precursors can be modified to various isoprenoid products through prenyl transferase, terpene synthase, or terpene cyclases. The production of prenylated aromatic compounds is achieved through prenyl transfer of the hydrocarbon units of isoprenoid precursors to polyketides.

This invention relates to the field of biomedical technology, and discloses an aromatic polyketide compound, its preparation method, and its applications. The aromatic polyketide compound is derived from... Crystallomyces hypericus Compound I and Compound II were isolated from the ethyl acetate extract of rice fermentation broth and identified as Crystalloketide A and Crystallomycin D, respectively. In vitro anticancer activity tests were conducted on Crystalloketide A and Crystallomycin D. The results showed that Compound I and Compound II inhibited human cervical cancer cells, human liver cancer cells, human neurocancer cells, and human pancreatic cancer cells, respectively. Therefore, it is further suggested that Compound I and Compound II described in this invention can be used to prepare anticancer drugs.

The present invention discloses a dimeric aromatic polyketide compound, a preparation method and an application thereof. The structural formula of the dimeric aromatic polyketide compound is shown in formula (I): The present invention is the first to isolate two novel dimeric aromatic polyketide compounds, spirocyclines A and B, from a rice fermentation product of the actinomycete Streptomyces spectabilis NA07643. These compounds have a novel ten-membered oxaspiro dimer skeleton, providing novel chemical entities for drug research and development.

Disclosed herein are compositions and methods of use thereof. For example, described herein are engineered 2-pyrone synthases, methods of use thereof for producing polyketides, polyketide precursors, polyketide derivatives, or a combination thereof, and compositions comprising the same.

The application belongs to the technical field of biological medicine, and particularly relates to a preparation method and application of a polyketide compound from a fungus of the genus Arenarius. The polyketide compound can dose-dependently reduce the NO generation level in cells, significantly inhibit M1 phenotype polarization and promote the conversion of the M1 phenotype to the M2 phenotype, significantly down-regulate the expression of key inflammatory-related proteins (including IL-1beta, IL-6, TNF-alpha and COX-2) in cells, and inhibit the generation of NLRP3 inflammasome and caspase-1, revealing the potential anti-AD activity and indicating that the polyketide compound has a good application prospect in the preparation of anti-neuroinflammatory drugs. In addition, the polyketide compound can be prepared from a marine microorganism Arenarius fungus, and the preparation method is simple and the source is rich.

A genetically modified microorganism for increasing the production of a polyketide compound or a derivative thereof is provided, including any one or both of the following genetic modifications: (a) a deleted endogenous pta gene encoding a phosphate acetyltransferase, wherein an expression level of the phosphate acetyltransferase of the deleted endogenous pta gene is lower than an expression level of its wild type; and (b) an added first exogenous nucleotide sequence and second exogenous nucleotide sequence, wherein the first exogenous nucleotide sequence encodes a transaminase and the second exogenous nucleotide sequence encodes a reductase. A method for producing a polyketide compound or a derivative thereof using the aforementioned genetically modified microorganism is also provided.