The application belongs to the technical field of
biology, and particularly discloses a method for synthesizing malonyl-CoA and application thereof. The method for synthesizing malonyl-CoA comprises the following steps: S1, converting an exogenous
carbon source substrate into 3-hydroxypropionic acid; and S2, converting the 3-hydroxypropionic acid obtained in S1 into malonyl-CoA. The application discloses a method for synthesizing malonyl-CoA and application thereof, and the method realizes efficient, stable and controllable production of malonyl-CoA and derivatives thereof, completely releases natural
feedback inhibition, and realizes stable and efficient synthesis.