21 results about "Polyketide synthase" patented technology

The invention discloses polyketide synthase or a polyketide synthase mutant and application thereof, and belongs to the technical field of gene engineering. The polyketide synthase is AspiPKS4, AspiPKS5, AspiPKS6 and AspiPKS7 from cyathea spinulosa, FhPKS2, FhPKS3, FhPKS4, FhPKS5 and FhPKS6 from ficus himalayana, and CrPKS from floating fern. The mutant is obtained by carrying out single point mutation on the second amino acid of AspiPKS7 or deleting an N-terminal redundant sequence. The polyketide synthase or the mutant is used for replacing NnHisspS in an original FBP luminescence system, and the biological self-luminescence level can be remarkably improved. The invention further provides a simplified and improved FBP system which comprises the polyketide synthase or the polyketide synthase mutant, CPH, H3H and Luz, and the application convenience and the application luminescence capacity of the FBP system are improved.

The invention provides application of a phellinus polyketide synthase gene SvPKS1 in promoting bioluminescence of fungi or plants, the coding sequence of the SvPKS1 is shown as SEQ ID NO.1, and the fungi are fungi for producing caffeic acid. According to the application, the SvPKS1 and other genes (nnH3H, nnLuz and nnCPH) of an FBP fungus luminescence pathway are co-expressed in fungi or plants by utilizing a transgenic technology, so that the fungi or plants are promoted to emit light biologically. The invention discloses co-expression of the phellinus polyketide synthase gene SvPKS1 and fungus luminescence pathway genes nnH3H, nnLuz and nnCPH for the first time, promotes fungus or plant bioluminescence, and provides a key candidate enzyme gene for promoting fungus or plant sustainable luminescence.

The present invention provides a chimeric polyketide synthase (PKS) or polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) and uses thereof, wherein the PKS or PKS-NRPS comprises two or more recombinant units (RUs) linked to each other, wherein each RU comprises one or two or more contiguous domains derived from a parent PKS or PKS-NRPS, and the junction of at least one RU is defined by a recombination site selected from a site at the LPTY (A / P) FQ (H / R) xRYWL motif located in the AT post- linker (RYWL motif site) and a site at the RL motif of ACP helix 0 (RL motif site); wherein the two or more RUs are not naturally linked to each other.

The application discloses a polyketide synthase mutant and application thereof, and belongs to the technical field of genetic engineering. The polyketide synthase mutant is obtained by deleting the N-terminal redundant sequence of AspiPKS7 from Cyathea. Replacing NnHispS in the original FBP light-emitting system with the polyketide synthase mutant can significantly improve the biological spontaneous light level. The application also provides a simplified and improved FBP system, which comprises the polyketide synthase mutant, CPH, H3H and Luz, and improves the application convenience and light-emitting capacity of the FBP system.

The application discloses a tylosin polyketide synthase acyltransferase mutant and application thereof, relates to the field of bioengineering, and comprises a first mutant, a second mutant or a third mutant; the amino acid sequences are shown in SEQ ID NO. 2, SEQ ID NO. 3 or SEQ ID NO. 4 respectively. The application discloses a coding gene of the mutant, a recombination carrier and a recombination bacteria and application thereof in catalyzing malonyl-CoA hydrolysis. The application discloses a soluble expression method of tylosin polyketide synthase acyltransferase domain TylAT5 protein, which comprises culturing bacterial bodies, eluting target proteins and further purifying the target proteins by using fast protein liquid chromatography. The application provides a recombination escherichia coli which can efficiently express acyltransferase mutants, and the efficiency of catalyzing malonyl-CoA of the recombination escherichia coli is 2.6 times higher than that of a wild type.

This invention discloses a strain of *Bacillus belyceae* with broad-spectrum antibacterial activity and its application in the prevention and treatment of necrotic enteritis in poultry. The *Bacillus belyceae* strain provided by this invention (… Bacillus from Velez The genome of strain GDG25CB056 is approximately 3.91 Mb in size, contains no virulence factors, and is rich in gene clusters encoding non-ribosomal peptides and polyketide synthases. Studies have confirmed that this strain exhibits significant growth advantages, strong tolerance to high temperatures, gastric acid, and bile salts, which facilitates intestinal colonization. It can produce siderophores and various enzymes, and has overcome the technical bottleneck of a narrow antibacterial spectrum, possessing broad-spectrum antibacterial activity, especially strong specific antagonistic activity against Clostridium perfringens. In vivo experiments have shown that this strain can significantly reduce the Clostridium perfringens load in the cecum of chicks with necrotizing enterocolitis, alleviate intestinal congestion and swelling, repair damaged intestinal villi, and enhance mucosal barrier function, providing a safe and efficient strain resource for the prevention and treatment of bacterial enteric diseases.

This application relates to the field of genetically engineered bacteria technology, specifically disclosing a genetically engineered *E. coli* strain for producing emodin, its construction method, and its applications. This application uses *E. coli* BAP1 as the substrate strain. This strain carries its own sfp in its genome, which can be used to activate polyketide synthase. Furthermore, *E. coli* itself grows rapidly, has a vigorous metabolism, and the culture medium is low-cost, facilitating high-density fermentation. Through gene editing, the uptake and utilization of glucose are improved, the flux of acetic acid and lactic acid diverted from pyruvate is reduced, and the ATP supply is enhanced to increase the flux of acetyl-CoA and malonyl-CoA within the bacteria. This provides sufficient substrate for the synthesis of emodin by polyketide synthase, thioesterase, and decarboxylase, thereby increasing the yield of emodin. The emodin yield achieved by this application through shake-flask fermentation reaches 576.4 mg / L, which is currently the highest level in the *E. coli* system.

This application relates to the technical fields of synthetic biology and enzyme engineering, and in particular to a computational-experimental closed-loop rational design method for polyketide synthases. The method includes: constructing a full-length FCHS model using AlphaFold3; identifying binding hotspots and verifying dynamic stability through molecular docking and kinetic simulations; and constructing a low-redundancy mutant library containing A, L, and F substitutions. This application solves the problems of difficult protein structure analysis and limited experimental throughput through a closed-loop process of computational pre-screening, dynamic verification, and evolutionary filtering, providing an efficient paradigm for the improvement of complex multifunctional enzymes.

The invention provides recombinant corynebacterium glutamicum for producing beta-arbutin as well as a construction method and application of the recombinant corynebacterium glutamicum, and belongs to the technical field of genetic engineering, and the recombinant corynebacterium glutamicum for producing beta-arbutin is obtained by expressing a beta-arbutin synthetic gene on the basis of a corynebacterium glutamicum inactivated polyketide synthase gene. The polyketide synthase gene is inactivated in corynebacterium glutamicum, the strain 13032-delta pks13 with enhanced biofilm forming ability is successfully obtained, the adsorption growth ability of corynebacterium glutamicum cells on the surfaces of various media can be effectively enhanced, beta-arbutin synthetic gene is over-expressed in the strain 13032-delta pks13 as a fermentation strain, and the biofilm forming ability of the strain 13032-delta pks13 is enhanced. And the application of efficient production of beta-arbutin is realized in an adsorption immobilization mode.

The invention provides a method for heterologous synthesis of a compound Angulotetrone for resisting phytophthora parasitica var nicotianae, which is characterized in that non-reduced polyketide synthase Atn1, UbiA type isopentenyl transferase Atn2, flavin dependent monooxygenase Atn3 and terpene cyclase Atn4 are co-expressed in an aspergillus oryzae host, so that a silent biosynthesis process is successfully activated; according to the invention, a novel antibacterial compound Angulotetrone is found and synthesized. The compound cannot be obtained in the original strain. Structural identification and activity tests show that the compound Angulotetrone shows remarkable inhibitory activity on phytophthora parasitica var nicotianae, and the minimum inhibitory concentration (MIC) is 9.01 g / mL. The invention not only provides a new candidate drug for prevention and treatment of tobacco black shank, but also provides an efficient technical platform for mining dark substances of microbial genomes and discovering active natural products with novel structures.

The application discloses a spinosyn polyketide synthase acyltransferase mutant and application thereof, relates to the field of bioengineering, and comprises a first mutant, a second mutant or a third mutant; the amino acid sequences are shown in SEQ ID NO. 2, SEQ ID NO. 3 or SEQ ID NO. 4 respectively. The application discloses a coding gene, a recombination carrier and a recombination bacteria of the mutant and application of the mutant in production of spinosyn A with high purity and single component. The application discloses a soluble expression method of the acyltransferase domain SpnAT8 protein of the spinosyn polyketide synthase module eight, which comprises culturing bacteria, eluting the target protein and further purifying the target protein by using fast protein liquid chromatography. The four-point mutant provided by the application has the activity of recognizing malonyl-CoA restored to 19.5% compared with the wild type, and almost loses the activity of recognizing methylmalonyl-CoA.

This invention relates to a method for increasing the yield of polyketide compounds by splitting a modular polyketide synthase gene. (1) Constructing a RedEx gene cassette for splitting the modular polyketide synthase gene; (2) Constructing a target plasmid carrying an amp-ccdB gene selection expression cassette; (3) Constructing a target plasmid carrying an amp-ccdB gene selection expression cassette. C DD-TGA-RBS-ATG- N (3) Target plasmid encoding the DD coding sequence; (4) Construction of recombinant strains; (5) Fermentation culture to obtain polyketide compounds. This invention is the first to discover that the biosynthetic efficiency of host strains carrying the split PKS gene is much higher than that of host strains carrying the natural PKS gene, which can achieve the purpose of increasing the yield of polyketide compounds. Using this method of splitting modular polyketide synthase genes, high-yield butenyl spinosad genetically engineered bacteria and high-yield avermectin genetically engineered bacteria were successfully constructed, which effectively increased the yield of butenyl spinosad and avermectin. The yield of butenyl spinosad increased by a maximum of 30 times, and the yield of avermectin increased by a maximum of 5.8 times.

The invention belongs to the technical field of biological enzymes and microorganisms, and particularly relates to a chimeric enzyme and application thereof in synthesis of isocoumarin compounds. Specifically, a polyketide synthase coding gene ColB in the Chaetomium olivum SD-80A and a polyketide synthase coding gene TerA from Aspergillus terreus are used for constructing the chimeric enzyme in a structural domain replacement manner for the first time, heterologous expression of the chimeric enzyme coding gene ColB-TerA-IV is realized by constructing a heterologous expression cassette, the function of the chimeric enzyme is determined, and the polyketide synthase coding gene ColB in the Chaetomium olivum SD-80A and the polyketide synthase coding gene TerA derived from Aspergillus terreus are used for preparing the polyketide synthase. The biosynthetic pathway of the fungus metabolite isocoumarin compound normal sporine is expanded, and lead compounds can be provided for novel herbicides and related drugs, so that the method has good practical application value.

This invention provides a *Phellinus linteus* polyketide synthase gene. SvPKS1 Applications in promoting bioluminescence in fungi or plants, the SvPKS1 The coding sequence is shown in SEQ ID NO.1, and the fungus is a caffeic acid-producing fungus. The application includes using transgenic technology to... SvPKS1 Other genes in the FBP fungal luminescence pathway ( nnH3H , nnLuz , nnCPH This invention discloses for the first time the *Phellinus linteus* polyketide synthase gene, which is co-expressed in fungi or plants to promote bioluminescence in fungi or plants. SvPKS1 With fungal luminescence pathway genes nnH3H , nnLuz , nnCPH Co-expression promotes bioluminescence in fungi or plants, providing a key candidate enzyme gene for promoting sustainable bioluminescence in fungi or plants.

The present invention relates to arecombinant microbial host cell producing a tetraketide or derivatives thereof from one or more substrates selected from cinnamoyl-CoA, p-Coumaroyl-CoA, Caffeoyl-CoA, Feruloyl-CoA, malonyl-CoA, sinapoyl-CoA and dihydro derivatives thereof, comprising an operative biosynthetic metabolic pathway for the tetraketide or derivatives thereof comprising a chalcone isomerase-like (CHIL) polypeptide heterologous to the host cell and a Type 3 polyketide synthase (PKS).

The application provides a kind of recombinant corynebacterium glutamicum for producing beta- arbutin and its construction method and application, belong to genetic engineering technical field, the recombinant corynebacterium glutamicum for producing beta- arbutin, is inactivated polyketide synthase gene on the basis of corynebacterium glutamicum again expression beta- arbutin synthesis gene after being obtained.The application inactivates polyketide synthase gene in corynebacterium glutamicum, and successfully obtains the strain 13032- Δpks13 with enhanced biofilm formation ability, can effectively enhance the adsorption growth ability of corynebacterium glutamicum cell on the surface of various media, overexpresses beta- arbutin synthesis gene in 13032- Δpks13 strain as fermentation strain, to realize the application of high-efficiency production of beta- arbutin in adsorption immobilization mode.

The application discloses a kind of polyketide synthase or polyketide synthase mutant and application thereof, belong to genetic engineering technical field.The polyketide synthase is AspiPKS4, AspiPKS5, AspiPKS6, AspiPKS7 of source of Cyatheales, FhPKS2, FhPKS3, FhPKS4, FhPKS5, FhPKS6 of source of leaf fig, CrPKS of source of water fern;The mutant is obtained by single-point mutation to the second amino acid of AspiPKS7 or N-terminal redundant sequence is deleted.The polyketide synthase or mutant replaces NnHispS in original FBP luminescent system, and the level of biological spontaneous light can be significantly improved.The application also provides a kind of simplified improved FBP system, includes the polyketide synthase or polyketide synthase mutant, CPH, H3H, Luz, improves the application convenience and application luminous capacity of FBP system.

This invention belongs to the field of bioenzyme and microbial technology, specifically relating to a chimeric enzyme and its application in the synthesis of isocoumarin compounds. Specifically, this invention is the first to utilize olive-colored hairy shells (… Chaetomium olivaceum A polyketide synthase encoding gene in SD-80A ColB With Aspergillus terreus ( Aspergillus terreus A polyketide synthase encoding gene from ( ) TerA Chimeric enzymes were constructed using domain substitution, and heterologous expression cassettes were built to achieve expression of the chimeric enzyme encoding gene. ColB‑TerA‑Ⅳ The heterologous expression of this chimeric enzyme confirmed its function, expanded the biosynthetic pathway of orthosporain, a fungal metabolite, and helped provide lead compounds for novel herbicides and related drugs, thus having good practical application value.

A sorbic acid synthetic gene cluster and synthetic method. Provided is any one of the following substances: A1) a polyketide synthase; A2) a biological material related to the polyketide synthase of A1); A3) a hydrolase; A4) a biological material related to the hydrolase of A3); A5) a combination of A1) and A3); and A6) a combination of A2) and A4). A novel sorbic acid biosynthetic gene cluster, which has not been reported in currently disclosed documents, is found, it is the first time that a biosynthetic gene cluster containing a polyketide synthase gene saA and a hydrolase gene saB is heterologously expressed to synthesize sorbic acid, which has reference significance for green biomanufacturing of sorbic acid.

The invention discloses a biosynthetic gene promoter litC of a biosynthetic gene of a deep sea fungus P. lithocarpus FS508 and an application of the biosynthetic gene promoter litC of the biosynthetic gene of the biosynthetic gene of the deep sea fungus P. lithocarpus FS508. The nucleotide sequence of the promoter is as shown in SEQ ID NO. 1. According to the invention, an upstream promoter sequence of a litC gene is obtained through amplification from an FS508 genome, and a core region of the promoter is predicted, so that a core sequence Plitc of the deep-sea fungus polyketide synthase gene litC promoter is obtained. The litC promoter disclosed by the invention can efficiently start expression of a hygromycin resistance gene hph and a G418 resistance gene NeoR in yeast, the efficiency of the litC promoter is better than that of a constitutive promoter TEF1, and the litC promoter shows broad-spectrum resistance gene starting activity and substitution potential. The result provides a new screening tool for a yeast expression system, and lays a foundation for improving the yield of the istocarpins and developing derivative compounds through a synthetic biological strategy.

The invention belongs to the technical field of genetically engineered bacteria, and particularly discloses an anti-penicillium velezensis genetically engineered bacterium as well as a preparation method and application thereof. According to the genetically engineered bacterium, a polyketide synthase gene cluster in a genome of bacillus velezensis is knocked out or inactivated, and the gene cluster comprises one of PKS5, PKS6 and PKS10 gene clusters. Through specific knockout of a polyketide synthase gene cluster competing with a target product, the metabolic flux is redistributed while the excellent growth performance of the strain is reserved, the yield of the anti-penicillium active substance is remarkably improved, and a new technical scheme is provided for solving the bottleneck of low strain titer in the existing biological control technology. Compared with an original strain, the bacillus subtilis has the advantages that the utilization efficiency of nutrient substances and the adaptability to the fermentation environment in industrial production are enhanced, the inhibition effect on penicillium is improved by 10.4%, and the bacillus subtilis has a good application prospect in the aspect of increasing the yield of industrial anti-penicillium active substances.