82results about How to "Has broad-spectrum antimicrobial activity" patented technology

The invention discloses water for removing acne and controlling oil and a production process thereof. The water is mainly prepared from material components such as water, EDTA (ethylene diamine tetraacetic acid) disodium, glycerin, salicylic acid, triethanolamine, butylene glycol, Carbopol, preservatives, PEG (polyethylene glycol)-60 hydrogenated castor oil, decamethylene glycol, hamamelis virginiana extract, calendula extract, tea tree leaf oil, medicinal salvia officinalis extract, plum-leaf crab seed oil and the like in a certain proportion. Aiming at the defects of existing water for removing acne and controlling oil, the invention researches and develops the water for removing acne and controlling oil, which can be used for effectively inhibiting excessive grease secretion and improving sebum keratinization conditions by using effective components of a plant extract. The water for removing acne and controlling oil can be used for effectively promoting toxins and inflammation removal, regulating acne skin, recovering neutral skin, promoting wound healing, decomposing, converting and discharging pigments, preventing the skin from being oxidized again by free radicals and black melanin to form acne marks, fading the acne marks, and preventing acne from recurring.

The invention relates to Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application of Pseudomonas chlororaphis to prevention and treatment of diseases attacking fruit trees and field crops, belonging to the field of biotechnology. The Pseudomonas chlororaphis with wide-spectrum antimicrobial activity is classified and named as Pseudomonas chlororaphis YTBTa14, with an accession number of CCTCC No. M2016099, and the 16SrDNA sequence of the Pseudomonas chlororaphis is as shown in a sequence table in the specification. The thallus and fermentation broth of the Pseudomonas chlororaphis with wide-spectrum antimicrobial activity in the invention both have good wide-spectrum antimicrobial activity to fungal diseases attacking fruit trees, have long-lasting and stable antimicrobial effect, show great potential in development into novel high-efficiency wide-spectrum bio-control fungicides, and provide a novel approach for application of microbes to biological control.

The invention discloses an anticorrosive component-free composition having an antibiosis effect. The composition comprises, by weight, 20-45% of p-hydroxyacetophenone, 3-10% of glycerol caprylate, 6-15% of caprylhydroxamic acid, 21-40% of propylene glycol, 0.5-1.5% of caprylyl glycol and 5-15% of ethylhexylglycerin, and the sum of the weight percentages of all above components is 100%. The anticorrosive component-free composition having the antibiosis effect has the advantages of no irritation, non-allergy, protruding antibiosis effect, harmlessness to human bodies, and no toxic or side effects, and can be safely applied to cosmetics. The invention also discloses a preparation method of the anticorrosive component-free composition having the antibiosis effect. The preparation method of the composition has the advantages of few steps, simple flow, and safety in operation. The invention further discloses an essence and a preparation method thereof. The essence has the advantages of good antibiosis effect, wide antibiosis range, no toxicity, harm or irritation, and high use safety, and the preparation method of the essence has the advantages of few steps, simple flow, and safety in operation.

The invention relates to marine streptomyces S187 having broad-spectrum antibacterial activity, belonging to the microbiological actinomycetes field. The marine streptomyces S187 is separated from a sea mud sample at a depth of 20 meters underwater in coastal waters. Primary screening through an agar block method proves that the bacterial strain has very strong antibacterial activity to Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger; the result of fermentation liquid secondary screening confirms good activity for antagonizing the Escherichia coli, the Staphylococcus aureus and the Bacillus subtilis. The analysis for 16S rDNA shows that the only actinomycetes having the homology as high as 99 percent with the marine streptomyces S187 is the salt lake bacterial strain Streptomyces chungwhensis (DSM41483) separated by Korean scholars, but the bacterial strain is separated from the sea mud sample for the first time. I type polyketide synthase (PKSI) is successfully amplified out from the streptomyces S187, which indicates that the streptomyces S187 has the potential ability of generating polyketide.

The invention discloses an aquilaria sinensis fruit skin extract, a preparation method thereof and application thereof. The method comprises the following steps of: crushing aquilaria sinensis fruit skin serving as a raw material, adding water, ethanol, ethanol water, ethyl acetate, trichloromethane or petroleum ether serving as a solvent, performing reflux extraction, ultrasonic extraction or cold extraction to obtain extracting solution, and performing concentration drying under reduced pressure or freeze drying on the extracting solution to obtain the aquilaria sinensis fruit skin extract. The aquilaria sinensis fruit skin extract has broad-spectrum antimicrobial activity, so that the extract can be applied to a food preservative and the preparation of antibacterial medicaments or antifungal medicaments. The aquilaria sinensis fruit skin extract has a simple preparation method, low costs and abundant raw materials. Therefore, the utilization rate and economic benefit of aquilaria sinensis are improved.

The invention discloses a skin wound dressing with the antimicrobial property and a preparation method of the skin wound dressing, belonging to the technical field of biomedical engineering. The skin wound dressing provided by the invention is of the spongy porous membrane structure, specifically, a sodium alginate fibrous membrane is prepared by taking sodium alginate as a base material through a freeze drying method, then biological antimicrobial peptide Cys-KR12 is fixed to the fibrous membrane through a surface finishing method, and thus the skin wound dressing is prepared. The skin wound dressing prepared by adopting the method provided by the invention is simple in preparation method and easily available in raw materials, the defects that the drug tolerance is generated, the functions are single, and the like after the existing antimicrobial dressing is used for a long term are overcome, and the skin wound dressing provided by the invention has low immunogenicity, good biocompatibility, good antimicrobial property and good induction repairing function, can promote the healing of the wound surface and the growth of epithelial cells, and is beneficial for the re-epithelialization of the wound surface.

The invention belongs to the technical field of cosmetics, and discloses a dry-type film containing antibacterial peptide and an epidermal growth factor, as well as a preparation method and application. The dry-type film mainly comprises the following active ingredients by weight percentage: 0.05-2.0% of antibacterial peptide, 0.001-0.2% of the epidermal growth factor, 0.01-0.4% of sodium hyaluronate, 0.05-1.0% of dipotassium glycyrrhizinate, 0.05-2.0% of sericin powder and 0.02-1.0% of Beta-glucan. The preparation method comprises the following steps: preparing dry film essence; spraying the dry film essence onto a dry film active ingredient carrier; stoving; packaging. Since the dry-type film does not contain moisture, the growth of fungi is inhibited; chemical preservatives are avoided, the side effect is reduced, and the dry-type film is safe, environment-friendly and long in guarantee period; the weight of a single dry-type film is reduced by 70-85%, and the dry-type film is low in transportation cost, convenient to carry and convenient to use.

The present invention discloses a new Hermetia illucens L antibacterial peptide StomoxynZH1, a preparation method and an application thereof. Preparation steps of the antibacterial peptide comprise: extracting the total RNA of insect Hermetia illucens L as a template, performing RT-PCR to obtain a first strand of cDNA as a template, and performing PCR amplification to obtain a PCR product; carrying out digestion to obtain a PCR product and a eukaryotic expression vector to construct a recombinant expression vector; transforming the resulting recombinant expression vector into yeast host cellsto obtain a recombinant strain expressing the antibacterial peptide StomoxynZH1; and carrying out inducing culture on the resulting recombinant strain to obtain a culture, and carrying out centrifugal separation on the culture to obtain the expressed antibacterial peptide. The antibacterial peptide of the present invention has broad spectrum antimicrobial activities and provides good application prospects in medicine, agriculture disease prevention and control, feed additives and other fields, wherein the broad spectrum antimicrobial activities comprise gram-negative bacteria escherichia coliresistance, gram-positive bacteria staphylococcus aureus resistance, and fungi rice blast pathogenic bacteria resistance.

The invention provides a hand sanitizer for children. The hand sanitizer is prepared from components in parts by mass as follows: 15-37 parts of a surfactant, 0.5-4 parts of skin moisturizing ingredients, 0.1-0.4 parts of a preservative, 0.1-1 part of a chelating agent, 0.5-2 parts of a thickening agent, 0.01-1 part of a pH regulator and the balance of deionized water. The provided hand sanitizer for children adopts a mild formula, has good detergency and also has unique skin care, sterilization and environment-friendly properties, no essence is added, natural flavor of tea tree essential oil is used, and the hand sanitizer is harmless to a human body after being used for a long time. A bottle in the shape similar to a penguin is used, a penguin inserting piece is inserted, a 3D effect is realized, and the modeling is lovely and novel.

The invention provides a feed additive, a preparation method and application thereof and relates to the technical field of livestock and poultry breeding. The feed additive is prepared from the following components: beta-glucanase (3.0-5.0)*105 U / kg, beta-mannanase (0.5-1.0)*105 U / kg, xylanase (4.4-6.6)*106 U / kg, cellulase (3.0-6.0)*104U / kg, bacillus subtilis greater than or equal to 6000 CFU / kg,bacillus licheniformis greater than or equal to 4000 CFU / kg, bacillus coagulans greater than or equal to 5000 CFU / kg, lactobacillus plantarum greater than or equal to 4000 CFU / kg, clostridium butyricum greater than or equal to 6000 CFU / kg and antimicrobial peptide of 5% or above. The feed additive can improve the utilization rate of daily ration of livestock and poultry and improve the immunity oflivestock and poultry. The use of the feed additive for livestock and poultry feed can significantly improve the production performance of livestock and poultry, reduce the cost of feed, and also reduce the use rate of antibiotics, and the additive is environmentally friendly.

The invention discloses an application of a rifamycin-nitroimidazole coupling molecule shown in formula I in restraining anaerobic flora. The rifamycin-nitroimidazole coupling molecule shown in formula I provided by the invention has a broad-spectrum antibacterial activity to anaerobion, has an activity to most pathogenic bacteria related to bacterial vaginosis, has a better in vitro antimicrobial activity than that of bacterial vaginosis drugs, such as, metronidazole and clindamycin, and has an excellent application prospect at the aspect of preventing and treating the bacterial vaginosis or the other related diseases caused by the anaerobion.

The invention relates to an antibacterial peptide derivative from a forest frog and application thereof and belongs to the technical field of biochemical polypeptide medicines. The polypeptide is a small-molecule polypeptide which is designed artificially and synthesized chemically by taking a natural antimicrobial peptide from the skin secretion of a forest frog as a template. The polypeptide comprises 18 amino acid residues and has a sequence as shown in a sequence table. The molecular weight of the polypeptide is 2542.9Da, and the isoelectric point is 12.6. Experiments show that the small-molecule polypeptide has a broad-spectrum effect of killing a variety of bacteria and has no hemolytic activity in an antibacterial and anticancer concentration range, and the bacteria can not become resistant to the small-molecule polypeptide. In addition, Experiments on animals show that the polypeptide has the function of resisting wound infection. The polypeptide has a small molecular weight, can be synthesized artificially and conveniently, is a small-molecule polypeptide with quite high application value and can be used for preparing novel antimicrobial and antiinfectious medicines.

The invention belongs to the technical field of livestock breeding, in particular to an ecological pig raising method. The ecological pig raising method comprises the following steps: establishment of pig houses; feeding and stocking of pigs. The ecological pig raising method is capable of reducing application of antibiotics and medicine residuals. Therefore, ecological pigs with good quality and taste are provided.

The invention relates to a jerusalem artichoke blade phenolic extract, as well a preparation method and application of the jerusalem artichoke blade phenolic extract. The preparation method comprises the following steps of: drying fresh jerusalem artichoke blades, and crushing the fresh jerusalem artichoke blades to obtain dry powder of the jerusalem artichoke blades; putting the dry powder of the jerusalem artichoke blades into a decompression reflux extraction device, adding de-ionized water, extracting at room temperature to obtain a water phenolic extract, and concentrating or drying the water phenolic extract; or, adding an organic solvent into the decompression reflux extraction device, extracting while refluxing or decompressing and refluxing, and concentrating or drying to obtain an organic solvent phenolic extract; and extracting the water phenolic extract of the jerusalem artichoke blades or the organic solvent phenolic extract by using petroleum ether, chloroform, ethyl acetate and n-butyl alcohol respectively in turn according to the volume ratio in a stepwise manner, and finally, concentrating and drying to obtain the stepwise phenolic extract. The extract disclosed by the invention belongs to the novel plant-source (vegetative) pesticide, has specially good effects on a plurality of plant pathogenic bacteria, such as botrytis cinerea, phytophthora capsici leonian, colletotrichum gloeosporioides, rhizoctonia cerealis and gaeumannomyces graminis, and also can be used as a natural plant-source fungicide and a preservation preservative of fruits and vegetables.

The invention provides an application of an antibacterial peptide as a preservative in preparing cosmetics. The antibacterial peptide provided by the invention has the beneficial effects that 1) the antibacterial peptide provided by the invention has broad-spectrum antibacterial activity and can effectively inhibit Gram negative bacteria, Gram positive bacteria, saccharomycetes and moulds; 2) the antibacterial peptide has good compatibility with raw materials of almost cosmetics, and the bacteriostatic ability is not affected by surfactants, proteins and Chinese herbal medicine additives in the cosmetics; and 3) the product does not contain a methanol releaser, highly irritant organic halogen components or chemical synthetic components, so that the product is high in safety and low in irritation.

The invention discloses a skin care cosmetic, particularly relates to an anti-allergy regenerative mask fluid. The fluid comprises, by weight percentage, 30%-60% of beta-glucan, 0.1%-0.5% of notoginsenoside, 0.01%-0.1% of astaxanthin, 0.1%-0.5% of Dipotassium Glycyrrhizate, 0.01%-0.2% of thickener, 0.1%-0.5% of methyl-p-hydroxy benzoate, 0.01%-0.05% of GM-BP and the balance being water. Natural plant extracts are adopted as the main ingredients for the fluid, and are specially selected to solve the problems of post-sunburn skin regeneration, allergy and moisture-keeping, wherein the beta-glucan not only has the function of keeping the skin moisture, but also can improve the skin elasticity and the ability to resist external stimulation, the notoginsenoside can nourish blood and alleviate pain, promote the regeneration of texture and inhibit inflamation. The astaxanthin is a natural anti-oxidant, and can reinforce the regenerative ability of cells and clean accumulated aging cells. The Dipotassium Glycyrrhizate has the anti-allergy and anti-stimulation effect with good percutaneous absorption effect.

The invention belongs to the technical field of biology and relates to a preparation method and application of a novel antimicrobial peptide Misgurin mutant. The technical scheme is as follows: introducing glutamine at the C tail end of the amino acid sequence of natural Misgurin so as to design a novel Misgurin mutant; selecting Pichia yeast biased codons so as to artificially synthesize a complete gene of the novel Misgurin mutant; constructing a Pichia yeast secretion type expression vector pPICZaA-M; transforming a host cell SMD1168 by a vector electroporation method; selecting to obtain high-efficiency expression strains; and under proper culture conditions, ensuring 100 microgram of recombinant novel Misgurin mutant is contained in per milliliter of expression supernatant. The obtained product has a good nematocidal activity on staphylococcus aureus, pig salmonella and pig pathogenic Escherichia coli K88, K99 and 987P, and has an obvious effect on prevention and treatment of clinical bacterial diseases.

The invention relates to the technical field of veterinary drugs, and specifically relates to a traditional Chinese medicine perfusate for treating cow mastitis as well as a preparation and application method thereof. The traditional Chinese medicine perfusate comprises the following materials in parts by weight: 1 part of a folium cortex eucommiae extract, and 1-3 parts of tea tree essential oil, wherein the folium cortex eucommiae extract and the tea tree essential oil are mixed to form mixed oil; auxiliary materials are added into the mixed oil to prepare the perfusate; the auxiliary materials include soybean oil, glycerinum, glycerin monostearate, ethylparaben, cyclodextrin, vaseline and distilled water. According to the traditional Chinese medicine perfusate, the folium cortex eucommiae extract is used as the effective component, so that the traditional Chinese medicine perfusate has the effects of clearing away heat and toxic materials, fighting bacteria and diminishing inflammation, and has an outstanding effect on treating clinical cow mastitis; the tea tree essential oil is compounded, thus the antibacterial activity of the traditional Chinese medicine perfusate is improved, and moreover, the permeability is greatly improved; the traditional Chinese medicine perfusate does not contain any chemical medicine components, so that the safety of milk products can be ensured; and the drug tolerance is hardly caused.

The invention provides an antibacterial peptide containing two leucine repeating units as well as a preparation method and an application. The amino acid sequence of the antibacterial peptide containing two leucine repeating units is shown by SEQ No.1. The preparation method comprises the following steps: obtaining the amino acid sequences of the leucine repeating units according to the sequence motifs of the leucine repeating units and the amino acid composition characteristics of the antibacterial peptide, and connecting the repeating units in series to increase the length of peptide chain and the number of net charges; synthesizing a polypeptide crude product by a solid-phase synthesis method through using a polypeptide synthesizer; and finally, purifying the synthesized polypeptide with high performance liquid chromatography, and identifying with electrospray ionization mass spectrometry. The antibacterial peptide provided by the invention has the beneficial effects that (1) the polypeptide is designed based on the leucine repeating units widely available in organism and has broad-spectrum antibacterial activity; and (2) the polypeptide consists of 22 amino acids and has relatively short peptide chain; and the preparation method and technology are mature, and the synthesis cost is low.

The invention belongs to the field of gel preparation and relates to high-efficiency bacteriostatic antibacterial peptide HC-30 gel which is modified hydrophis cyanocinctus antibacterial peptide Hc-CATH and a preparation method thereof. The high-efficiency bacteriostatic antibacterial peptide HC-30 gel is prepared from 0.2-0.7% by mass of antibacterial peptide, 0.25-0.5% by mass of a gel base, 1-3% by mass of a transdermal absorption promoter, 10-30% by mass of a humectant and a proper amount of a pH conditioning agent and purified water. The high-efficiency bacteriostatic antibacterial peptide HC-30 gel can be prepared by simple processes, has good stability, is safe and nontoxic, has no irritation, can be easily coated and removed, can fast produce sterilization effects on the skin surface and has an application potential.

The invention belongs to the technical field of biology, and particularly relates to bacteriocin PE-ZYB1 with preservation and fresh-keeping effects for food and application thereof. The bacteriocin PE-ZYB1 is obtained by separation and purification from lactic acid bacteria zy-B by a four-step method using ethyl acetate extraction, Sephadex LH-20 column chromatography system purification, ion exchange chromatography purification and semi-preparative reversed-phase liquid chromatography purification. The bacteriocin PE-ZYB1 has an amino acid sequence as shown in SEQ ID NO: 1, and is similar toan antibacterial peptide Andersonin-X1 with similarity of only 38.09%. The bacteriocin PE-ZYB1 has broad-spectrum antibacterial activity against both gram-positive and gram-negative food-borne pathogens and spoilage bacteria, has high heat resistance and stability at high temperature, can still maintain good bacteriostatic activity at a pH value of 2-7, especially has a killing effect on Listeriamonocytogenes, and can be used in the field of food preservation and fresh-keeping.

The present invention discloses a perilla flavone and perilla flavone derivative extraction method comprising the following steps: (1) selecting perilla leaves, grinding and breaking with a mortar, and sieving; (2) putting the ground and sieved perilla leaves into an ultrasonic extraction machine tank, and adding 30-80% by mass of ethanol, wherein the solid-liquid ratio is 1: 30-50, the water bath temperature is 40-80 DEG C, water bath time is 0.5-2.5 hours, and ultrasound time is 20-60min, (3) centrifuging the mixture obtained by the step 1to2 at 12000g, and removing the precipitate to obtain a perilla flavone and perilla flavone derivative solution. The yield of the perilla flavone and perilla flavone derivative extracted by the method is 78.2mg / g. The obtained perilla flavone has broad-spectrum antimicrobial activity, has good gram negative bacteria and gram positive bacteria antibacterial activity, has the 24h inhibition rate reaching 90% or more, and has broad prospects for development.

The invention discloses a Polygonatum sibiricum extract-containing amino acid perfumed soap and a processing technology thereof. The perfumed soap comprises, by weight, 15-20 parts of cocoyl sarcosine, 10-15 parts of palmitoyl glutamic acid, 5-10 parts of dipalmitoyl hydroxyproline, 20-30 parts of lanolin, 4-8 parts of Polygonatum sibiricum extract, 3-6 parts of phytosteryl / octyldodecyl lauroyl glutamate, 2.5-5 parts of triethanolamine, 10-14 parts of sodium hydroxide, 1-5 parts of gamma-polyglutamic acid, 10-15 parts of propylene glycol, 1-3 parts of sorbitol, 1-3 parts of hirudin, 1-2 partsof antibacterial peptides and 20-25 parts of water; and the active substances of the Polygonatum sibiricum extract at least include 250 mg / g of Polygonatum sibiricum polysaccharides, 50 mg / g of Polygonatum sibiricum saponin and 18 mg / g of Polygonatum sibiricum flavones. The amino acid perfumed soap has the advantages of fine foam, high skin-friendliness, high safety, good anti-oxidation property,good hard water resistance, non-deformability and high stability.

The invention discloses an actinomycin compound as well as a preparation method and application thereof. A streptomycete used for producting the actinomycin compound is Streptomyces sp.S011 and is preserved in CGMCC (China general microbiological culture collection center) on June 6, 2018, and the preservation No. is CGMCC No.15910. The chemical structural formula of the actinomycin compound is shown as the formula (I) in the original specification. The method for producing the actinomycin compound, which is provided by the invention, comprises a step of fermenting the streptomycete S011; an actinomycin D and the actinomycin compound (a demethylation actinomycin B) can be produced in a large scale through industrial fermentation, wherein the demethylation actinomycin B is a new compound, the two compounds have good antibacterial property and cytotoxic activity, therefore, the method is wide in application prospect.

The invention discloses a novel broad-spectrum antimicrobial peptide SAMP1-A4 and a preparing method thereof. The antimicrobial peptide has the amino acid sequence of NH2-VRLLRRRI-COOH. The preparingmethod is a solid-phase polypeptide synthesis method protected by amino acid nitrogen Fmoc. The defect that a frequently-seen antibacterial peptide is poor in biological safety, prone to hemolysis ortoxic to mammal cells is overcome, and the antimicrobial peptide is a novel antimicrobial peptide which has the broad-spectrum restraining effect on non-filamentous fungi and bacteria, has no hemolysis and is free of toxin to normal mammal cells. The peptide provides a novel primer for clinical antifungal medicine research.

The invention discloses a compound with a synergistic effect on phenazino-1-carboxylic acid as well as a composition and an application of the compound. The compound is selected from one or more of protoberberine alkaloid, derivatives of the protoberberine alkaloid or medicinal salts of the protoberberine alkaloid. The compound disclosed by the invention can generate a remarkable synergistic effect on the inhibition effect of the compound on tea tree fungi under the condition of a low dosage of phenazino-1-carboxylic acid.

The invention discloses a novel broad-spectrum antibacterial peptide SAMP1-A3 and a preparation method thereof. The amino acid sequence of the antibacterial peptide is NH2-RKKKVLLI-COOH. The preparation method of the invention adopts a solid-phase polypeptide synthesis means protected by amino acid nitrogen Fmoc. The method overcomes the defects of poor biological safety, easy hemolysis or toxicity to mammalian cells of common antibacterial peptides, and the novel antibacterial peptide has broad-spectrum inhibition effect on non-filamentous fungi and bacteria, does not have hemolysis and has no toxicity to normal mammalian cells. The polypeptide provides a novel leader for clinical antifungal drug research.

The invention discloses an application of a rifamycin-quinolizidone double target molecule shown in formula I in inhibition of anaerobic flora. The rifamycin-quinolizidone double target molecule shown in formula I has broad spectrum antimicrobial activity comprising activity for anaerobic bacteria including most pathogenic bacteria of vaginae, has the in-vitro antibacterial activity superior to that of metronidazole, clindamycin and other first-tier bacterial vaginosis resisting drugs and has good application prospects in the aspects of prevention and treatment of bacterial vaginosis or other related diseases caused by anaerobic bacteria.