82results about How to "Has broad-spectrum antimicrobial activity" patented technology

The invention discloses water for removing acne and controlling oil and a production process thereof. The water is mainly prepared from material components such as water, EDTA (ethylene diamine tetraacetic acid) disodium, glycerin, salicylic acid, triethanolamine, butylene glycol, Carbopol, preservatives, PEG (polyethylene glycol)-60 hydrogenated castor oil, decamethylene glycol, hamamelis virginiana extract, calendula extract, tea tree leaf oil, medicinal salvia officinalis extract, plum-leaf crab seed oil and the like in a certain proportion. Aiming at the defects of existing water for removing acne and controlling oil, the invention researches and develops the water for removing acne and controlling oil, which can be used for effectively inhibiting excessive grease secretion and improving sebum keratinization conditions by using effective components of a plant extract. The water for removing acne and controlling oil can be used for effectively promoting toxins and inflammation removal, regulating acne skin, recovering neutral skin, promoting wound healing, decomposing, converting and discharging pigments, preventing the skin from being oxidized again by free radicals and black melanin to form acne marks, fading the acne marks, and preventing acne from recurring.

The invention relates to Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application of Pseudomonas chlororaphis to prevention and treatment of diseases attacking fruit trees and field crops, belonging to the field of biotechnology. The Pseudomonas chlororaphis with wide-spectrum antimicrobial activity is classified and named as Pseudomonas chlororaphis YTBTa14, with an accession number of CCTCC No. M2016099, and the 16SrDNA sequence of the Pseudomonas chlororaphis is as shown in a sequence table in the specification. The thallus and fermentation broth of the Pseudomonas chlororaphis with wide-spectrum antimicrobial activity in the invention both have good wide-spectrum antimicrobial activity to fungal diseases attacking fruit trees, have long-lasting and stable antimicrobial effect, show great potential in development into novel high-efficiency wide-spectrum bio-control fungicides, and provide a novel approach for application of microbes to biological control.

The invention discloses an anticorrosive component-free composition having an antibiosis effect. The composition comprises, by weight, 20-45% of p-hydroxyacetophenone, 3-10% of glycerol caprylate, 6-15% of caprylhydroxamic acid, 21-40% of propylene glycol, 0.5-1.5% of caprylyl glycol and 5-15% of ethylhexylglycerin, and the sum of the weight percentages of all above components is 100%. The anticorrosive component-free composition having the antibiosis effect has the advantages of no irritation, non-allergy, protruding antibiosis effect, harmlessness to human bodies, and no toxic or side effects, and can be safely applied to cosmetics. The invention also discloses a preparation method of the anticorrosive component-free composition having the antibiosis effect. The preparation method of the composition has the advantages of few steps, simple flow, and safety in operation. The invention further discloses an essence and a preparation method thereof. The essence has the advantages of good antibiosis effect, wide antibiosis range, no toxicity, harm or irritation, and high use safety, and the preparation method of the essence has the advantages of few steps, simple flow, and safety in operation.

The invention relates to marine streptomyces S187 having broad-spectrum antibacterial activity, belonging to the microbiological actinomycetes field. The marine streptomyces S187 is separated from a sea mud sample at a depth of 20 meters underwater in coastal waters. Primary screening through an agar block method proves that the bacterial strain has very strong antibacterial activity to Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger; the result of fermentation liquid secondary screening confirms good activity for antagonizing the Escherichia coli, the Staphylococcus aureus and the Bacillus subtilis. The analysis for 16S rDNA shows that the only actinomycetes having the homology as high as 99 percent with the marine streptomyces S187 is the salt lake bacterial strain Streptomyces chungwhensis (DSM41483) separated by Korean scholars, but the bacterial strain is separated from the sea mud sample for the first time. I type polyketide synthase (PKSI) is successfully amplified out from the streptomyces S187, which indicates that the streptomyces S187 has the potential ability of generating polyketide.

The invention discloses an aquilaria sinensis fruit skin extract, a preparation method thereof and application thereof. The method comprises the following steps of: crushing aquilaria sinensis fruit skin serving as a raw material, adding water, ethanol, ethanol water, ethyl acetate, trichloromethane or petroleum ether serving as a solvent, performing reflux extraction, ultrasonic extraction or cold extraction to obtain extracting solution, and performing concentration drying under reduced pressure or freeze drying on the extracting solution to obtain the aquilaria sinensis fruit skin extract. The aquilaria sinensis fruit skin extract has broad-spectrum antimicrobial activity, so that the extract can be applied to a food preservative and the preparation of antibacterial medicaments or antifungal medicaments. The aquilaria sinensis fruit skin extract has a simple preparation method, low costs and abundant raw materials. Therefore, the utilization rate and economic benefit of aquilaria sinensis are improved.

The present invention discloses a new Hermetia illucens L antibacterial peptide StomoxynZH1, a preparation method and an application thereof. Preparation steps of the antibacterial peptide comprise: extracting the total RNA of insect Hermetia illucens L as a template, performing RT-PCR to obtain a first strand of cDNA as a template, and performing PCR amplification to obtain a PCR product; carrying out digestion to obtain a PCR product and a eukaryotic expression vector to construct a recombinant expression vector; transforming the resulting recombinant expression vector into yeast host cellsto obtain a recombinant strain expressing the antibacterial peptide StomoxynZH1; and carrying out inducing culture on the resulting recombinant strain to obtain a culture, and carrying out centrifugal separation on the culture to obtain the expressed antibacterial peptide. The antibacterial peptide of the present invention has broad spectrum antimicrobial activities and provides good application prospects in medicine, agriculture disease prevention and control, feed additives and other fields, wherein the broad spectrum antimicrobial activities comprise gram-negative bacteria escherichia coliresistance, gram-positive bacteria staphylococcus aureus resistance, and fungi rice blast pathogenic bacteria resistance.

The invention provides a hand sanitizer for children. The hand sanitizer is prepared from components in parts by mass as follows: 15-37 parts of a surfactant, 0.5-4 parts of skin moisturizing ingredients, 0.1-0.4 parts of a preservative, 0.1-1 part of a chelating agent, 0.5-2 parts of a thickening agent, 0.01-1 part of a pH regulator and the balance of deionized water. The provided hand sanitizer for children adopts a mild formula, has good detergency and also has unique skin care, sterilization and environment-friendly properties, no essence is added, natural flavor of tea tree essential oil is used, and the hand sanitizer is harmless to a human body after being used for a long time. A bottle in the shape similar to a penguin is used, a penguin inserting piece is inserted, a 3D effect is realized, and the modeling is lovely and novel.

The invention provides a feed additive, a preparation method and application thereof and relates to the technical field of livestock and poultry breeding. The feed additive is prepared from the following components: beta-glucanase (3.0-5.0)*105 U/kg, beta-mannanase (0.5-1.0)*105 U/kg, xylanase (4.4-6.6)*106 U/kg, cellulase (3.0-6.0)*104U/kg, bacillus subtilis greater than or equal to 6000 CFU/kg,bacillus licheniformis greater than or equal to 4000 CFU/kg, bacillus coagulans greater than or equal to 5000 CFU/kg, lactobacillus plantarum greater than or equal to 4000 CFU/kg, clostridium butyricum greater than or equal to 6000 CFU/kg and antimicrobial peptide of 5% or above. The feed additive can improve the utilization rate of daily ration of livestock and poultry and improve the immunity oflivestock and poultry. The use of the feed additive for livestock and poultry feed can significantly improve the production performance of livestock and poultry, reduce the cost of feed, and also reduce the use rate of antibiotics, and the additive is environmentally friendly.

The invention relates to an antibacterial peptide derivative from a forest frog and application thereof and belongs to the technical field of biochemical polypeptide medicines. The polypeptide is a small-molecule polypeptide which is designed artificially and synthesized chemically by taking a natural antimicrobial peptide from the skin secretion of a forest frog as a template. The polypeptide comprises 18 amino acid residues and has a sequence as shown in a sequence table. The molecular weight of the polypeptide is 2542.9Da, and the isoelectric point is 12.6. Experiments show that the small-molecule polypeptide has a broad-spectrum effect of killing a variety of bacteria and has no hemolytic activity in an antibacterial and anticancer concentration range, and the bacteria can not become resistant to the small-molecule polypeptide. In addition, Experiments on animals show that the polypeptide has the function of resisting wound infection. The polypeptide has a small molecular weight, can be synthesized artificially and conveniently, is a small-molecule polypeptide with quite high application value and can be used for preparing novel antimicrobial and antiinfectious medicines.

The invention belongs to the technical field of livestock breeding, in particular to an ecological pig raising method. The ecological pig raising method comprises the following steps: establishment of pig houses; feeding and stocking of pigs. The ecological pig raising method is capable of reducing application of antibiotics and medicine residuals. Therefore, ecological pigs with good quality and taste are provided.

The invention provides an application of an antibacterial peptide as a preservative in preparing cosmetics. The antibacterial peptide provided by the invention has the beneficial effects that 1) the antibacterial peptide provided by the invention has broad-spectrum antibacterial activity and can effectively inhibit Gram negative bacteria, Gram positive bacteria, saccharomycetes and moulds; 2) the antibacterial peptide has good compatibility with raw materials of almost cosmetics, and the bacteriostatic ability is not affected by surfactants, proteins and Chinese herbal medicine additives in the cosmetics; and 3) the product does not contain a methanol releaser, highly irritant organic halogen components or chemical synthetic components, so that the product is high in safety and low in irritation.

The invention discloses a skin care cosmetic, particularly relates to an anti-allergy regenerative mask fluid. The fluid comprises, by weight percentage, 30%-60% of beta-glucan, 0.1%-0.5% of notoginsenoside, 0.01%-0.1% of astaxanthin, 0.1%-0.5% of Dipotassium Glycyrrhizate, 0.01%-0.2% of thickener, 0.1%-0.5% of methyl-p-hydroxy benzoate, 0.01%-0.05% of GM-BP and the balance being water. Natural plant extracts are adopted as the main ingredients for the fluid, and are specially selected to solve the problems of post-sunburn skin regeneration, allergy and moisture-keeping, wherein the beta-glucan not only has the function of keeping the skin moisture, but also can improve the skin elasticity and the ability to resist external stimulation, the notoginsenoside can nourish blood and alleviate pain, promote the regeneration of texture and inhibit inflamation. The astaxanthin is a natural anti-oxidant, and can reinforce the regenerative ability of cells and clean accumulated aging cells. The Dipotassium Glycyrrhizate has the anti-allergy and anti-stimulation effect with good percutaneous absorption effect.

The invention relates to the technical field of veterinary drugs, and specifically relates to a traditional Chinese medicine perfusate for treating cow mastitis as well as a preparation and application method thereof. The traditional Chinese medicine perfusate comprises the following materials in parts by weight: 1 part of a folium cortex eucommiae extract, and 1-3 parts of tea tree essential oil, wherein the folium cortex eucommiae extract and the tea tree essential oil are mixed to form mixed oil; auxiliary materials are added into the mixed oil to prepare the perfusate; the auxiliary materials include soybean oil, glycerinum, glycerin monostearate, ethylparaben, cyclodextrin, vaseline and distilled water. According to the traditional Chinese medicine perfusate, the folium cortex eucommiae extract is used as the effective component, so that the traditional Chinese medicine perfusate has the effects of clearing away heat and toxic materials, fighting bacteria and diminishing inflammation, and has an outstanding effect on treating clinical cow mastitis; the tea tree essential oil is compounded, thus the antibacterial activity of the traditional Chinese medicine perfusate is improved, and moreover, the permeability is greatly improved; the traditional Chinese medicine perfusate does not contain any chemical medicine components, so that the safety of milk products can be ensured; and the drug tolerance is hardly caused.

The invention belongs to the technical field of biology, and particularly relates to bacteriocin PE-ZYB1 with preservation and fresh-keeping effects for food and application thereof. The bacteriocin PE-ZYB1 is obtained by separation and purification from lactic acid bacteria zy-B by a four-step method using ethyl acetate extraction, Sephadex LH-20 column chromatography system purification, ion exchange chromatography purification and semi-preparative reversed-phase liquid chromatography purification. The bacteriocin PE-ZYB1 has an amino acid sequence as shown in SEQ ID NO: 1, and is similar toan antibacterial peptide Andersonin-X1 with similarity of only 38.09%. The bacteriocin PE-ZYB1 has broad-spectrum antibacterial activity against both gram-positive and gram-negative food-borne pathogens and spoilage bacteria, has high heat resistance and stability at high temperature, can still maintain good bacteriostatic activity at a pH value of 2-7, especially has a killing effect on Listeriamonocytogenes, and can be used in the field of food preservation and fresh-keeping.

The present invention discloses a perilla flavone and perilla flavone derivative extraction method comprising the following steps: (1) selecting perilla leaves, grinding and breaking with a mortar, and sieving; (2) putting the ground and sieved perilla leaves into an ultrasonic extraction machine tank, and adding 30-80% by mass of ethanol, wherein the solid-liquid ratio is 1: 30-50, the water bath temperature is 40-80 DEG C, water bath time is 0.5-2.5 hours, and ultrasound time is 20-60min, (3) centrifuging the mixture obtained by the step 1to2 at 12000g, and removing the precipitate to obtain a perilla flavone and perilla flavone derivative solution. The yield of the perilla flavone and perilla flavone derivative extracted by the method is 78.2mg / g. The obtained perilla flavone has broad-spectrum antimicrobial activity, has good gram negative bacteria and gram positive bacteria antibacterial activity, has the 24h inhibition rate reaching 90% or more, and has broad prospects for development.

The invention discloses a novel broad-spectrum antimicrobial peptide SAMP1-A4 and a preparing method thereof. The antimicrobial peptide has the amino acid sequence of NH2-VRLLRRRI-COOH. The preparingmethod is a solid-phase polypeptide synthesis method protected by amino acid nitrogen Fmoc. The defect that a frequently-seen antibacterial peptide is poor in biological safety, prone to hemolysis ortoxic to mammal cells is overcome, and the antimicrobial peptide is a novel antimicrobial peptide which has the broad-spectrum restraining effect on non-filamentous fungi and bacteria, has no hemolysis and is free of toxin to normal mammal cells. The peptide provides a novel primer for clinical antifungal medicine research.

The invention discloses a novel broad-spectrum antibacterial peptide SAMP1-A3 and a preparation method thereof. The amino acid sequence of the antibacterial peptide is NH2-RKKKVLLI-COOH. The preparation method of the invention adopts a solid-phase polypeptide synthesis means protected by amino acid nitrogen Fmoc. The method overcomes the defects of poor biological safety, easy hemolysis or toxicity to mammalian cells of common antibacterial peptides, and the novel antibacterial peptide has broad-spectrum inhibition effect on non-filamentous fungi and bacteria, does not have hemolysis and has no toxicity to normal mammalian cells. The polypeptide provides a novel leader for clinical antifungal drug research.