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Perilla flavone and perilla flavone derivative extraction method

A technology of flavonoids and extraction methods in perilla, which is applied in the field of extraction of flavonoids and derivatives in perilla, and can solve the problems of few reports of flavonoids in perilla

Inactive Publication Date: 2016-01-27
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are more and more studies on the extraction and application of perilla active substances, but the current research on perilla is mainly concentrated on perilla oil, and there are relatively few reports on perilla flavonoids

Method used

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Effect test

Embodiment 1

[0039] According to the extraction method of the technical scheme of the content of the invention, using ethanol as a solvent, the influence of ethanol volume (30%, 40%, 50%, 60%, 70%, 80%) on the yield of flavonoids was investigated respectively. The concentration of flavonoids was determined using rutin standard curve with absorbance. The formula for calculating the yield of flavonoids is:

[0040] Yield of total flavonoids in perilla = (total amount of flavonoids in crude extract / mass of perilla leaf) × 100%, the following calculation method is the same.

[0041] Depend on figure 2 It can be seen that there is a curvilinear relationship between ethanol concentration and flavonoid yield, and the yield of flavonoid extracted by different concentrations of ethanol is quite different. The yield of flavonoids increased sharply when the ethanol concentration was 30%-40%; the yield was relatively stable when the concentration was 40%-60% (the difference was only 0.06%); the ext...

Embodiment 2

[0043] According to the extraction method of the technical scheme of the content of the invention, using ethanol as a solvent, the influence of the ratio of ethanol to liquid (1:20, 1:30, 1:40, 1:50) on the yield of flavonoids was investigated respectively. The calculation formula and calculation method are the same as above. Depend on image 3 It can be seen that with the increase of solid-liquid ratio, the yield of flavonoids gradually increased. When the ratio of solid to liquid was 1:40, the highest yield of total flavonoids was 3.32%. When the ratio of solid to liquid was 1:50, the extraction rate decreased slightly. Therefore, 1:30, 1:40, and 1:50 are selected as the conditions for the orthogonal test.

Embodiment 3

[0045] According to the extraction method of the technical scheme of the content of the invention, different ultrasonic treatment times (20, 30, 40, 50, 60 min) were selected to investigate the influence on the yield of flavonoids.

[0046] Depend on Figure 4 It can be seen that the yield of flavonoids gradually increases when the ultrasonic treatment time is 20-40. Because the cavitation and oscillation of ultrasonic waves are beneficial to the dissolution of intracellular substances. However, the extraction rate began to decrease when the ultrasonic treatment time exceeded 40 min, which may be because the structure and biological activity of flavonoids were destroyed by long-term ultrasonic treatment. Therefore, an ultrasonic time of 30 to 50 min was selected for orthogonal experiments.

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Abstract

The present invention discloses a perilla flavone and perilla flavone derivative extraction method comprising the following steps: (1) selecting perilla leaves, grinding and breaking with a mortar, and sieving; (2) putting the ground and sieved perilla leaves into an ultrasonic extraction machine tank, and adding 30-80% by mass of ethanol, wherein the solid-liquid ratio is 1: 30-50, the water bath temperature is 40-80 DEG C, water bath time is 0.5-2.5 hours, and ultrasound time is 20-60min, (3) centrifuging the mixture obtained by the step 1to2 at 12000g, and removing the precipitate to obtain a perilla flavone and perilla flavone derivative solution. The yield of the perilla flavone and perilla flavone derivative extracted by the method is 78.2mg / g. The obtained perilla flavone has broad-spectrum antimicrobial activity, has good gram negative bacteria and gram positive bacteria antibacterial activity, has the 24h inhibition rate reaching 90% or more, and has broad prospects for development.

Description

technical field [0001] The invention relates to the technical field of chemistry, in particular to a method for extracting flavonoids and derivatives thereof from perilla. Background technique [0002] Perilla frutescens, also known as Perilla frutescens, is a labiatae herb that has been cultivated in my country for more than 2,000 years. It is one of the first batch of traditional medicine and food dual-purpose plants promulgated by the Ministry of Health[1-3] . Studies have shown that multiple parts of perilla are rich in nutrients and active substances. Among them, perilla stems and leaves contain 8 kinds of essential amino acids for human body. The oil content of perilla seeds is 30%-51%, and most of them are unsaturated fatty acids, of which α-linolenic acid is 64.73%, linoleic acid is 15.43%, and oleic acid is 12.01%, which is an ideal edible oil raw material. Perilla leaf extract has anti-inflammatory and antipruritic effects. [0003] At present, there are more an...

Claims

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Application Information

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IPC IPC(8): A61K36/535A61P39/06
Inventor 张启焕李凤伟仇明王资生许伟邵荣
Owner YANCHENG INST OF TECH
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