15results about How to "Active" patented technology

The application discloses a Sinonovacula constricta glycoprotein nano-enzyme Sc-NZ and a preparation method thereof, and particularly relates to the technical field of nano-enzymes. The Sinonovacula constricta glycoprotein nano-enzyme Sc-NZ is a cluster-shaped nanoparticle obtained by treating a product separated and purified from Sinonovacula constricta under ultrahigh pressure, and the particle size distribution is 30-50 nm. The preparation method comprises the following steps: taking soft body tissue of Sinonovacula constricta, homogenizing and defatting, centrifuging to collect the precipitate to obtain defatted powder, extracting the defatted powder by an alkali extraction and alcohol precipitation method, adjusting the pH to 4.0-5.0, collecting and drying the precipitate to obtain a crude glycoprotein; subjecting the crude glycoprotein to enzymolysis, and then sequentially subjecting the glycoprotein to anion exchange chromatography and gel filtration chromatography to collect an active component to obtain a Sinonovacula constricta natural glycoprotein; adding deionized water or a phosphate buffer to the Sinonovacula constricta natural glycoprotein to prepare a solution, and placing the solution in an ultrahigh pressure device to obtain the Sinonovacula constricta glycoprotein nano-enzyme Sc-NZ. The Sinonovacula constricta glycoprotein nano-enzyme Sc-NZ has the advantages of being prepared by green physical modification, high activity, high stability and multifunction.

A polishing pad conditioner and polishing apparatus, wherein the polishing pad conditioner comprises a conditioning disc adapted to contact a polishing pad to condition the polishing pad; a conditioning disc mechanical arm located on a side of the conditioning disc facing away from the polishing pad, the conditioning disc mechanical arm being adapted to drive the conditioning disc to move; and a laser detection module fixed to the conditioning disc mechanical arm, the laser detection module being adapted to emit and receive a probe light to determine a position of the conditioning disc. The position of the conditioning disc is determined by the laser detection module, so as to determine whether the conditioning disc is in contact with the polishing pad. When it is determined that the conditioning disc is not in contact with the polishing pad, i.e. the conditioning disc has not polished the surface of the polishing pad, the position of the conditioning disc is adjusted in time so that the conditioning disc is lowered to be in contact with the surface of the polishing pad, thereby ensuring that the polishing pad is conditioned by the conditioning disc before polishing a wafer, so as to improve the activity of the surface of the polishing pad and improve the polishing effect of the polishing pad on the wafer.

This invention discloses a high-performance meta-aramid / mica composite insulating paper and its preparation method. The paper is formed by wet papermaking and hot pressing of a mixed slurry composed of meta-aramid chopped fibers, meta-aramid precipitated fibers, and mica flakes. The composite insulating paper has a thermal decomposition temperature ≥425℃, tensile strength ≥52N / mm, tear strength ≥2.2N / mm, and voltage breakdown strength ≥13.8kV / mm. The mass ratio of chopped fibers to precipitated fibers is (50~80):(50~20). The amount of mica flakes added is 5wt%~10wt% of the total mass of the two aramid fibers. The hot pressing adopts a segmented hot pressing process of "low-temperature pre-pressing - medium-temperature strengthening - high-temperature setting". By controlling the key parameters of the precipitated fibers and optimizing the ratio of chopped fibers to precipitated fibers and the proportion of mica flakes added, the paper is prepared through processes such as raw material pretreatment, graded mixing and dissociation, wet papermaking, and segmented hot pressing. Advantages: Through multi-parameter coordinated control and process optimization, the technical bottlenecks such as the mechanical and insulation properties of existing aramid composite insulating paper and insufficient thermal stability have been solved.

The application discloses a kind of nanocomposite hydrogel for treating osteoarthritis and its preparation method and application, the nanocomposite hydrogel includes cerium dioxide nanoparticles, the cerium dioxide nanoparticles are modified with hyaluronic acid;Ergothioneine is loaded on hyaluronic acid modified cerium dioxide nanoparticles to form nanocomposite EGT-CeO2@HA;And temperature-sensitive hydrogel carrier, the nanocomposite EGT-CeO2@HA is uniformly dispersed in the temperature-sensitive hydrogel carrier.This composite hydrogel can activate ATF4-dependent pro-survival autophagy pathway, reduce cartilage cell inflammatory damage, and improve cartilage degeneration.

The application discloses a monoclonal antibody VacBB-551 for neutralizing SARS-CoV-2 in a broad spectrum and an application thereof, which is composed of a heavy chain and a light chain matched with the heavy chain; amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are 'EIIVSRNY', 'IYAGGST' and 'ARSLGDRFDF' in sequence, and amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are 'QGIPSY', 'AAS' and 'QHEDT' in sequence. The novel coronavirus monoclonal antibody VacBB-551 of the application has cross-neutralization and binding activity to nine kinds of novel coronaviruses including various subgroups of the Omicron variant, and the neutralization effect is better than that of the neutralizing antibody separated from an existing inactivated vaccine, thereby providing a new choice for prevention and treatment of the novel coronavirus.

The application discloses a coumarin O-isopentenyltransferase belonging to a UbiA family and an application thereof, the coumarin O-isopentenyltransferase gene is identified from a Rutaceae plant, and in addition, it is proved through experiments that the coumarin O-isopentenyltransferase gene can be stably expressed in a tobacco and an escherichia coli and the like heterologous system, and can efficiently catalyze the conversion of umbelliferone to generate auraptene; the advantage of the application lies in that, as the first identified O-isopentenyltransferase capable of catalyzing the synthesis of auraptene, the PtPT can be used as a key biological catalysis element for constructing an efficient heterologous synthesis system of auraptene, so that the biological manufacturing efficiency is significantly improved, and the application has good industrialization application potential.

This invention discloses a water-resistant magnesium phosphate repair mortar based on industrial waste residue and its preparation method, comprising mineral admixtures, potassium dihydrogen phosphate, magnesium oxide, quartz sand, organosilicon gel, retarder, and mixing water; the mineral admixtures are composed of steel slag, nickel slag, and fly ash. During preparation, potassium dihydrogen phosphate, retarder, and mixing water are first mixed and stirred, then the mineral admixtures, magnesium oxide, and organosilicon gel are added sequentially and stirred rapidly, and finally the quartz sand is added and stirred. The repair mortar of this invention exhibits a setting time ≥23 min, fluidity ≥195 mm, 3-hour compressive strength ≥25.2 MPa, 3-day compressive strength ≥47.4 MPa, 3-hour flexural strength ≥3.6 MPa, 3-day flexural strength ≥6.1 MPa, and 1-day tensile bond strength ≥0.8 MPa; and a water absorption ratio below 0.15.

This research relates to the biomedical field and describes a thermosensitive hydrogel loaded with ginger extracellular vesicles and tannic acid, its preparation method, and its application in the preparation of anti-infective drugs. The process includes extracting ginger-derived extracellular vesicles; loading tannic acid onto these vesicles to obtain TA@GDEVs; preparing SP hydrogels; and slowly adding TA@GDEVs to the SP hydrogel to form a GTSP hydrogel system. GDEVs exhibit anti-inflammatory and pro-repair activities; TA provides broad-spectrum antibacterial, anti-inflammatory, and antioxidant functions; the SP hydrogel endows the thermosensitive hydrogel composite system with injectable and in-situ gelation properties; the SP hydrogel not only provides in-situ gelation and a protective barrier for GDEVs and TA but also enables controlled release of the loaded material; through synergistic effects, the three components enable the thermosensitive hydrogel composite system to possess responsiveness to the infection microenvironment, enhanced antibacterial efficacy, immune remodeling, and tissue repair functions, demonstrating its potential as a multifunctional anti-infective wound repair material.

The application relates to the field of biotechnology and discloses a neutralizing antibody against a novel coronavirus as well as a preparation method, a preparation and an application thereof. The nanobody comprises complementarity determining regions CDR1, CDR2 and CDR3 as shown in SEQ ID NO. 2-4. The nanobody is clear in a recognition and binding mechanism with RBD, can be combined with SARS-CoV-2 efficiently and specifically, and the affinity can reach a picomolar level (Kd<1x10 ‑12 M); the nanobody recognizes and binds RBD in a different way from ACE2, still has good neutralizing biological activity (IC50: 16.37 nM), can obtain excellent effects in SARS-CoV-2 detection or diseases caused by SARS-CoV-2, and has a wide application prospect in the fields of biology and medicine.

The invention discloses a composite hydrogel dressing based on gradient freeze thawing and a preparation method and application thereof, and belongs to the technical field of biomedical materials.The preparation method of the composite hydrogel dressing comprises the following steps that a polyvinyl alcohol solution and a polyvinylpyrrolidone solution are mixed, and a binary composite precursor solution is obtained; mixing sericin powder with the binary composite precursor solution to obtain a ternary composite precursor solution; injecting the ternary composite precursor solution into a mold, and preparing by adopting a gradient freeze thawing method to obtain ternary composite hydrogel; and dipping the ternary composite hydrogel in a potassium guaiacol sulfonate solution to form the composite hydrogel dressing loaded with the potassium guaiacol sulfonate. The composite hydrogel dressing prepared by the invention has multiple functions of excellent mechanical properties, good biocompatibility, adhesion, drug release ability, antibacterial ability and the like, and can be used for wound healing.

The invention belongs to the technical field of biology, and particularly relates to an anti-novel coronavirus antibody or an antigen binding fragment thereof as well as a preparation method and application thereof. The antibody or the antigen binding fragment of the antibody can specifically recognize and bind the Omicro BA.4 / 5 type novel coronavirus or the RBD protein of the Omicro BA.4 / 5 type novel coronavirus, and has no cross reaction on wild type SARS-CoV-2, Delta (B.1.617.2), Omicro BA.1 or the RBD protein of the Omicro BA.1; the recombinant plasmid provided by the invention has good neutralizing activity on Omicro BA.4 / 5 type new coronavirus, Omicro XBB.1 type new coronavirus and / or Omicro BQ.1.1 type new coronavirus; the compound can be used for diagnosing, preventing and / or treating novel coronavirus infection or diseases caused by the novel coronavirus infection, detecting existence or level of the novel coronavirus or RBD protein of the novel coronavirus in a sample or developing or screening drugs for preventing and / or treating the novel coronavirus infection or the diseases caused by the novel coronavirus infection.

The invention relates to the technical field of functional food, in particular to a tendon-nourishing and bone-strengthening composition and a preparation method thereof, and the tendon-nourishing and bone-strengthening composition comprises a fermented mulberry leaf polyphenol-sea cucumber glycosamine polymer, clover leaf saponin, a selenium yeast-pachymaran compound, glucosamine, compound nuts, vitamin D, a beef-pork compound, a traditional Chinese medicine compound and cheese; the three functional components of the fermented mulberry leaf polyphenol-sea cucumber glycosamine polymer, the clover leaf saponin and the selenium yeast-pachymaran compound cooperate to effectively block the accumulation and toxic effect of harmful heavy metals in the Chinese herbal medicines in the human body, and normal absorption of trace elements necessary for the human body is not interfered at the same time.

The present invention provides an Agrobacterium KG3 microorganism, a composition and a kit comprising the microorganism, and a method for reducing the level of a nitrogen-containing substance in a medium containing a nitrogen-containing substance using the microorganism, the microorganism having a preservation number of KCTC16103BP. The Agrobacterium KG3 microorganism according to the present invention can treat a nitrogen-containing substance in a medium containing the nitrogen-containing substance in a simple single-step aerobic condition.

The invention provides a compound containing phosphaphenanthrene bonded benzocyclobutene as well as a preparation method and application of the compound. The compound containing phosphaphenanthrene bonded benzocyclobutene at least comprises a DOPO (9, 10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide) group and a BCB (benzocyclobutene) group. The compound containing phosphaphenanthrene bonded benzocyclobutene is prepared by reacting DOPO (9, 10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide) and / or a DOPO derivative with a benzocyclobutene derivative. The compound containing phosphaphenanthrene bonded benzocyclobutene is applied to a PCB (Printed Circuit Board), has excellent thermal stability, and can be used as a reactive flame retardant to carry out Diels-Alder reaction with unsaturated resin, so that the compound has excellent flame retardant property; and a key raw material is provided for development of a high-performance flame-retardant structural member material urgently needed in the high-end manufacturing field.

The invention discloses a cutinase mutant gene, a protein expressed by the cutinase mutant gene and application of the cutinase mutant gene. The protein expressed by the cutinase mutant gene can be correctly folded and can be massively purified in an escherichia coli system. Meanwhile, the mutant protein disclosed by the invention has the function of degrading PET (Polyethylene Terephthalate) by wild cutinase, and the catalytic efficiency of the protein expressed by the cutinase mutant gene is improved compared with that of a control group. The protein expressed by the keratin mutant gene has activity and stability at 40-80 DEG C. Specifically, when the pH value is 8.0 and the temperature is 72 DEG C, the PET degradation capacity is improved by 20% compared with that of a control group, and when the pH value is 8.0 and the temperature is 60 DEG C, the PET degradation capacity is improved by 70% compared with that of the control group.