8 results about "Fucosidase" patented technology

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention provides a preparation method of an antibody conjugate containing an sLeX tetrasaccharide structure, which comprises the following steps: S1, sequentially adding Endo S2 enzyme and alpha-L-fucosidase into a target antibody, and respectively analyzing glycosylation to avoid modification site conflict or carbohydrate chain structure interference; s2, adding the accurately represented antibody in the S1, B4GalT1 and UDP-Gal into a Tris-HCl system containing Mn < 2 + >, and incubating to obtain a reaction solution I; s3, the reaction liquid I, ST3Gal3, CMP-Neu5Ac and Tris-HCl containing Mg < 2 + > are taken and mixed, and incubation is carried out; adding CMP-N-acyl neuraminic acid phosphodiesterase for reaction, and purifying Protein A to obtain a purified antibody I; s4, the purified antibody I, alpha1, 3 FucT-2HR, GDP-Fuc and Tris-HCl containing Mg < 2 + > are taken and mixed, and incubation is performed; and purifying Protein A to obtain a target product. According to the modified antibody obtained by the invention, while the targeting property is improved, the binding affinity of the antibody and a tumor cell surface antigen can be enhanced, so that the immune blocking activity of the antibody is further enhanced, and dual improvement of the targeting property and the activity is realized.

This invention belongs to the field of plant protection and discloses the application of α-L-fucosidase MoFco1 in screening drugs for the control of rice blast, as well as the application of compound 0989 in the control of rice blast. This invention identifies α-L-fucosidase MoFco1, which is active against XXFG in rice blast fungus. Located in the apoplast space, it regulates the pathogenicity of the fungus by degrading hemicellulose oligosaccharides in rice cell walls, inhibiting host ROS accumulation, and regulating defense responses. Based on virtual screening of the MoFco1 structure predicted by AlphaFold3, compound 0989 was obtained. This compound specifically binds to MoFco1 and effectively inhibits the pathogenicity of rice blast fungus. This invention provides new targets and new agents for the green control of rice blast and has significant application value.

The present application relates to a kind of extraction kit of cartilage tissue single cell and its extraction method, the kit includes: (1) first enzymolysis enzyme, the first enzymolysis enzyme includes one or more of trypsin, chondroitinase ABC and fucosidase;(2) second enzymolysis enzyme, the second enzymolysis enzyme includes collagenase II, neutral protease II, hyaluronidase and Dnase I;(3) base liquid, the base liquid includes base medium and / or buffer solution.The present application also provides the method for extracting single cell from cartilage tissue using the kit.The cartilage tissue single cell suspension prepared by the present application has the advantages of high cell yield and high activity, and has wide application prospect.

A method for preparing fucose and intermediates thereof and applications thereof. In the method for synthesizing GDP-fucose, GDP-fucose is generated by catalytic reaction of sucrose synthase, CDP-threose 2-epimerase, GDP-mannose dehydratase and isomerase with guanosine diphosphate and sucrose as substrates. In the method for synthesizing fucosyllactose, fucosyllactose is obtained by catalytic action of Hp13FucT2 enzyme with GDP-fucose and pNP-lactose as initial substrates. In the method for preparing L-fucose by enzyme, L-fucose is generated by catalytic reaction of fucosidase with fucosyllactose as substrate. The E. coli expression system has the characteristics of high expression efficiency, large expression amount, low cost and easy operation, and the expressed fucosidase has the advantages of high purity and high yield.

The application discloses an alpha-L-fucosidase detection kit and relates to the field of kits.The alpha-L-fucosidase detection kit comprises a box body, a box cover is arranged on the top of the box body, a placing cavity is formed in the inside of the box body, a placing seat is fixedly arranged in the inside of the placing cavity, a moving seat is arranged above the placing seat, a plurality of reagent tubes are inserted between the moving seat and the placing seat, and a lifting assembly is arranged at the bottom of the moving seat.When a plurality of reagent tubes need to be used for experiment, the end of the inserting rod is separated from the upper inserting hole by pulling the inserting rod outward, the moving seat can be pulled upward at the moment, a plurality of reagent tubes can be moved upward by the moving strip at the moment, the identification on the plurality of reagent tubes is convenient to check, and the internal reagents in the plurality of reagent tubes can be detected.

The invention discloses acid-resistant alpha-L-fucosidase IDSFuc29-2 and application thereof, and belongs to the field of protease, the amino acid sequence of the alpha-L-fucosidase IDSFuc29-2 is as shown in SEQ ID No.2, the alpha-L-fucosidase IDSFuc29-2 is derived from a metagenome of bottom mud of the East China Sea, and recombinase with definite catalytic activity can be obtained through a prokaryotic expression system. The enzyme shows good reaction characteristics under the acidic condition, the optimum reaction pH of the enzyme is about 4.0, high relative enzyme activity can still be kept within the pH range of 4.0-6.0, and meanwhile, the enzyme has acceptable stability under the condition of 20-40 DEG C. The application conditions of the GH29 family enzyme in degradation and related application of fucoidin are expanded, and the GH29 family enzyme has important research significance and application value.