39results about How to "Strong broad-spectrum antibacterial activity" patented technology

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

The invention relates to a biogenic composite pork preservative. The biogenic composite pork preservative comprises 4.0-4.8% of a bacterial depressant, 10-14% of a synergist, 27-33% of Chinese herbal medicine extract A, 16-20% of Chinese herbal medicine extract B and the balance water. The bacterial depressant contains nisin, natamycin, lysozyme, chitosan and propolis supercritical CO2 extract. The synergist contains acetic acid, vitamin C, vitamin E and sodium lactate. The Chinese herbal medicine extract A is prepared through extracting grape seeds, garlic, clove, cassia bark, rosemary, pepper and ginger through an ethanol extraction method and then blending the extract products, Origanum vulgare L. essential oil and tea crude extract. The Chinese herbal medicine extract B is prepared through water extraction of konjak, Chinese honeylocust fruits, rheum officinale and pomegranate peel. The biogenic composite pork preservative has a reasonable formula, is safe for eating, can be prepared by simple processes, is convenient for operation in use and has good fresh-keeping effects.

The invention relates to Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application of Pseudomonas chlororaphis to prevention and treatment of diseases attacking fruit trees and field crops, belonging to the field of biotechnology. The Pseudomonas chlororaphis with wide-spectrum antimicrobial activity is classified and named as Pseudomonas chlororaphis YTBTa14, with an accession number of CCTCC No. M2016099, and the 16SrDNA sequence of the Pseudomonas chlororaphis is as shown in a sequence table in the specification. The thallus and fermentation broth of the Pseudomonas chlororaphis with wide-spectrum antimicrobial activity in the invention both have good wide-spectrum antimicrobial activity to fungal diseases attacking fruit trees, have long-lasting and stable antimicrobial effect, show great potential in development into novel high-efficiency wide-spectrum bio-control fungicides, and provide a novel approach for application of microbes to biological control.

The invention relates to the technical field of cosmetics, in particular to a skin whitening and anti-aging composition and a cosmetic. The skin whitening and anti-aging composition comprises amelanchier alnifolia nutt extract, green tea extract, centella asiatica extract and flos sophorae immaturus extract. The skin whitening and anti-aging composition and the cosmetic have the advantages that the functional components of the skin whitening and anti-aging composition and the cosmetic are carefully selected from natural extracted components of four types of plants, the activity of tyrosinase can be effectively inhibited after the natural extracted components are used in a combined manner, melanogenesis can be inhibited, free radicals in organisms can be obviously scavenged, strong antioxidant effects can be realized, and complementary and synergistic skin whitening and anti-aging effects can be realized.

The invention provides a biological deodorant spray for pets as well as a preparation method and an application of the biological deodorant spray. The biological deodorant spray for the pets is prepared from main raw materials as follows: a micro-ecological agent and plant extract, wherein the micro-ecological agent contains one or more of a bacillus subtilis solution, a bacillus licheniformis solution, a bacillus coagulans solution, a lactobacillus acidophilus solution, a enterococcus faecalis solution and saccharomyces cerevisiae solution; the plant extract contains one or more of yucca extract, aloe extract, agastache rugosa extract, honeysuckle extract, ginkgo biloba leaf extract and tea polyphenol. The deodorant spray is safe, green, non-toxic and residue-free, has fermentation aromaand cannot cause secondary pollution to the environment, meanwhile, under the joint action of the micro-ecological agent and the plant extract, stink of excrement can be effectively reduced, air is purified, and hair odor of the pets can be removed.

The invention provides a Scylla paramamosain antiviral type lipopolysaccharide resistant factor as well as a preparation method and application thereof and relates to an antiviral type lipopolysaccharide resistant factor. The invention provides a gene sequence and an amino acid sequence of a Scylla paramamosain antiviral type lipopolysaccharide resistant factor Sp-ALF2, a preparation method of the Scylla paramamosain antiviral type lipopolysaccharide resistant factor Sp-ALF2 and application of the Scylla paramamosain antiviral type lipopolysaccharide resistant factor Sp-ALF2. The preparation method comprises the following steps: constructing a recombinant expression vector of the Scylla paramamosain antiviral type lipopolysaccharide resistant factor; leading the recombinant expression vector into a host cell, carrying out inducing expression on the host cell so as to obtain the expression product; separating and purifying the expression product so as to obtain recombinant protein, namely Sp-ALF2. The Scylla paramamosain antiviral type lipopolysaccharide resistant factor Sp-ALF2 has obvious inhibition effect and killing effect on WSSV (white spot syndrome virus) and multiple pathogenic bacteria, and has wide application value in preparation of disease resistant new medicaments and the Scylla paramamosain antiviral type lipopolysaccharide resistant factor as an animal disease resistant additive also has wide application value.

The invention discloses infantile hand-mouth wet wipe, made with a detergent and nonwoven bearing the detergent; the detergent comprises the following components, by weight: 0.1-8 parts of a sweetener, 0.2-5 parts of a deterging agent, 0.2-8 parts of a plant extract, 1-5 parts of a moisturizer, 0.3-1.0 part of a preservative, 0.1-3 parts of a pH regulator and 90-99 parts of deionized water. The Infantile hand-mouth wet wipe is nontoxic, non-irritating, highly safe to use, soft and comfortable, suitable for wiping sensitive parts of a baby such as hand and mouth, also suitable for cleaning baby supplies such as nipples and feeding bottles. The invention also discloses a production method of the infantile hand-mouth wet wipe. The preparation method is simple, high in operational safety and high in efficiency.

The invention discloses a low foam lubricating liquid for a food beverage chain conveying belt, wherein 0.1-5% by weight of water-soluble emulsified silicone oil, 0.1-5% by weight of alkyl glucoside, 0.1-2% by weight of an anti-foaming lubricant, 0-0.5% by weight of an anti-rust agent, 0-1% by weight of a scale inhibitor, 0.2-1.0% by weight of a preservative, and the balance of water are mixed, and a pH value adjusting agent is added to adjust the pH value to 6.0-7.0 so as to obtain the low foam lubricating liquid. The low foam lubricating liquid has characteristics of good lubricity, good cleaning effect, good water solubility, low foam, no toxicity, no corrosion, good environmental compatibility, easy production, and convenient use.

The invention discloses a muscle therapy mineral bath salt. The muscle therapy mineral bath salt is prepared from the following components in parts by weight according to a conventional method: 800 parts of sea salt, 20 parts of C8-10 alkyl glucoside, 10 parts of sandalwood, 5 parts of clove, 15 parts of wrinkled giant hyssop, 2 parts of eucalyptus essential oil and 3 parts of rosemary essential oil. The muscle therapy mineral bath salt disclosed by the invention can effectively relieve muscle pain as well as whiten, exfoliate and moisturize the skin. The muscle therapy mineral bath salt is rich in pure natural traditional Chinese medicine raw materials, and can enable body skin to enjoy bath salt essential nourishing every day and take good care of every inch of the skin. The muscle therapy mineral bath salt is particularly suitable for people with neck and shoulder pain or various muscle pains.

The invention discloses a body-clearing and toxin-removing mineral bath salt. The body-clearing and toxin-removing mineral bath salt is prepared from the following components in parts by weight according to a conventional method: 800 parts of sea salt, 20 parts of C8-10 alkyl glucoside, 10 parts of cassia seed, 5 parts of senna leaf, 15 parts of mulberry leaf and 10 parts of liquorice root. The body-clearing and toxin-removing mineral bath salt disclosed by the invention can promote diuresis, remove toxins as well as whiten, exfoliate and moisturize the skin. The body-clearing and toxin-removing mineral bath salt is rich in pure natural traditional Chinese medicine raw materials, and can enable body skin to enjoy bath salt essential nourishing every day and take good care of every inch of the skin. The body-clearing and toxin-removing mineral bath salt is particularly suitable for people who are prone to growing acnes and blackheads on the backs.

The invention discloses an optical glass cleaning agent and a preparation method thereof. The optical glass cleaning agent is composed of the following components in percentage by mass: 14 to 30 percent of propylene glycol, 1 to 5 percent of coconut diethanol amide, 0.8 to 1.5 percent of sodium carboxymethyl cellulose, 15 to 22 percent of sodium gluconate, 3 to 10 percent of alkyl glucoside, 12 to18 percent of an inorganic alkaline substance and the balance of water. The optical glass cleaning agent disclosed by the invention has the advantages of strong wetting power, strong dirt removing power and good washing effect, can be used for dirt including oil dirt, wax, carbon black and the like stuck on the surface of glass and also has extremely strong removing power on cerium oxide and ethylene oxide; the optical glass cleaning agent has small corrosiveness on the surface of the glass and is easy to rinse when optical glass is cleaned; the dirt also can be effectively prevented from being deposited again through a chelating effect between the sodium carboxymethyl cellulose and the sodium gluconate; and raw materials are environmentally friendly, have no irritation and are easily biodegraded. The quality of the washed product completely meets the requirements of customers and the optical glass cleaning agent is suitable for being popularized and applied.

The invention relates to a preparation method of an application of an artificial antimicrobial peptide MA-D4. According to the invention, an amino acid sequence of the artificial antimicrobial peptide MA-D4 is designed artificially, and a nucleotide sequence encoding the antimicrobial peptide is designed by using an escherichia coli preference codon; a target gene is synthesized by an overlap region amplification gene splicing method, and a prokaryotic expression vector is constructed and is transformed into the escherichia coli BL21 or Rosetta for inducing expression; an expressed product is subjected to digestion treatment by enterokinase and is subjected to nickel ion affinity chromatography purification, so as to obtain the artificial antimicrobial peptide MA-D4. The antimicrobial peptide has higher broad-spectrum antibacterial activity, can inhibit gram-positive bacteria and gram-negative bacteria, and meanwhile, excludes hemolytic activity. Therefore, as a novel antimicrobial drug, the antimicrobial peptide has excellent application prospect on the aspects of food preservation, disease treatment and the like, and has very high developing potential. The preparation method is high in expression efficiency, easy in separation and purification, low in production cost and good in stability, therefore, the preparation method can be used for mass production.

The invention discloses a bubble liquid producing bubbles capable of being patted and a preparation method thereof. The bubble liquid comprises following substances: KOH, coconut oil, absolute ethyl alcohol, APG, ECS, 6501, liquid sodium polyacrylate, cane sugar, glycerin, distilled water, universal compound antibacterial agent and essence. Compared with the prior art, the bubble liquid of the invention is faint yellow, slightly dense, and fragrant; the blown bubbles have large number and moderate sizes are not liable to broke; the bubbles can be patted with blowing tools, have bright colors, and are natural and environmentally friendly which are suitable for children.

The invention discloses preparation of environment-friendly and moderate hand lotion. The environment-friendly and moderate hand lotion is prepared from the following ingredients in parts by weight: 14-18 parts of alkyl glycoside, 1-3 parts of NaCl, 1-2 parts of glycerine, 1-2 parts of plant extract I, 1-2 parts of plant extract II, 0.3-0.5 part of flavoring essence and the balance of water. Compared with the prior art, the preparation has the following advantages that the formula is simple; the hand lotion is non-toxic and harmless and is free of irritation to skin; the hand lotion has a good washing effect on hand dirt and has the effects of preserving moisture, replenishing water, inhibiting bacteria, and the like; the hand lotion is readily biodegrade and environmentally friendly.

The invention provides a bacteriostatic laundry gel bead and a preparation method thereof. Comprising the following components in percentage by mass: 30%-48% of sodium alcohol ether sulphate, 5%-16% of dodecylbenzene sulfonic acid, 10%-16% of fatty acid methyl ester ethoxylate, 0%-20% of alkyl glycoside, 5%-8% of glycerol monolaurate, 5%-15% of cocamidopropyl betaine, 1%-10% of triethanolamine and 3%-5% of a solvent. And the balance of water. Through formula adjustment, the adding amount of the fatty alcohol-polyoxyethylene ether sodium sulfate can be effectively increased, and bacteria such as staphylococcus aureus can be effectively inhibited.

The invention relates to anti-haze amino acid silicone-oil-free liquid shampoo and a preparation method thereof and belongs to the technical field of daily chemical products. Amino acids are producedby virtue of sericin hydrolysis, and lauryl chloride is introduced by bonding, so that a hydrophilic-lipophilic balance is reached, and the surface activity is improved; a sericin surfactant is prepared and then compounded with cocamidopropyl betaine, so that a synergistic effect between the two is achieved, a micellar structure of the sericin surfactant in the liquid shampoo is changed, and the viscosity of the liquid shampoo system is greatly improved; and moreover, hair is coated by the sericin, rough defects on the hair surface are filled up, a smooth protective film is formed, adhesion ofhaze particles is avoided, and problems of haze caused to the hair can be solved. Meanwhile, hair nutrition is supplemented by virtue of the amino acids produced by sericin hydrolysis, excellent wetting and moisturizing properties are provided by virtue of lots of hydrophilic groups, irritation and damage to the hair can be reduced, and the problems of dry hair, poor combability, dumb hair and the like caused by extremely high degreasing power of the liquid shampoo are solved.

The invention relates to the field of genetic engineering and discloses recombinant protein of pulmonary prosurfactant protein-B (proSP-B) as well as a preparation method and application of the recombinant protein. Specifically, the recombinant protein has the amino acid sequence shown in SEQ ID NO: 1, or an amino terminal and/or carboxyl terminal in SEQ ID NO: 1 is connected to an amino acid sequence with a tag. According to the method, the proSP-B recombinant protein is obtained by a genetic engineering method, has low cytotoxicity and shows good antibacterial activity, thereby having good practical application prospect.

The invention provides an alginic acid-chitooligosaccharide-zinc oxide composite hydrogel and its preparation method and application. The present invention uses alginic acid as a raw material, prepares oxidized alginic acid through an oxidant, then cross-links the aldehyde group after alginic acid oxidation with the amino group of chitosan oligosaccharide through Schiff base, and simultaneously loads zinc oxide nanoparticles to prepare alginic acid- Oligochitosan‑Zinc Oxide Composite Hydrogel. It is verified by experimental research that the composite hydrogel of the present invention has a porous three-dimensional structure, good mechanical properties, and realizes Zn ²⁺ slow release. The alginic acid-chitooligosaccharide-zinc oxide composite hydrogel not only has hydrophilicity, water retention and biocompatibility, but also has broad-spectrum antibacterial effect and can effectively promote wound healing. Therefore, the composite hydrogel shows obvious advantages in promoting wound healing, antibacterial infection and water retention, and has broad application prospects in tissue engineering applications.

The invention provides a polypeptide and a preparation method thereof, and relates to the technical field of biological pharmacy. Artificial design and modification are carried out on the basis of polypeptide from a natural source, the polypeptide shown in the amino acid sequence of SEQ ID NO.1-5 is provided, and the blood solubility of the polypeptide is reduced on the basis of keeping the biological activity of the polypeptide. The preparation method comprises the following steps: S1, artificially designing an amino acid sequence of the polypeptide and a nucleotide sequence for coding the amino acid sequence; S2, connecting the nucleotide sequence with an expression vector, transforming and/or transfecting into a host cell, and screening positive clones; S3, culturing positive clones, and inducing polypeptide expression; and S4, breaking the host cell, and purifying the protein polypeptide to obtain the polypeptide. The preparation method is high in expression efficiency, simple in separation and purification process and high in product purity, the purity of the obtained polypeptide can reach 98%, the pharmaceutical industrialization level is reached, the production cost is low, and the preparation method is suitable for large-scale industrial production.