174results about How to "Expanded antimicrobial spectrum" patented technology

The invention discloses a plant extract and synergist contained antimicrobial composition. The composition contains a plant extract (A), a synergist (B), a mildew preventive (C), an antiseptic (D) and a solvent (E). The composition disclosed by the invention has a good inhibiting effect on bacteria and fungi (molds nod saccharomycetes); through the synergistic effect of all the components, the consumption of the traditional chemical antiseptic IPBC (Iodopropynyl Butylcarbamate) or nipagin esters is remarkably reduced, the safety in using the composition is improved, the irritation is reduced, and the possible risks are reduced. Meanwhile, the antiseptic is compounded in the composition, so that the antibacterial spectrum of an antiseptic system of the composition is effectively expanded, and the bacteriostatic ability is enhanced.

The invention relates to a formula of an aquatic product composite preservative. The formula of the aquatic product composite preservative is characterized in that the aquatic product composite preservative in each liter of distilled water comprises the following components: 0.4 to 0.6 g of muramidase, 2.5 to 3.5 g of tea polyphenol, 16 to 20 g of carboxymethyl chitosan, 10 to 14 g of nisin, 25 to 35 g of potassium sorbate, 17 to 23 g of sodium alginate, 20 to 30 g of lactobacillus, 1.5 to 2.5 g of propolis extracts and 10 to 14 g of spice extracts; and the spice extracting liquid comprises the raw materials: galangal, garlic, onion, cinnamon, clove and rosemary. The aquatic product composite preservative provided by the invention is prepared by compounding biological preservatives, is safe and non-toxic, has simple preparation and application methods, prominent microbial inhibition effect and good preservation effect, can greatly prolong the shelf life of frozen aquatic products, and has important industrial value.

The invention relates to the technical field of medical apparatus and instruments, which discloses a sustained release type antibacterial medical catheter and a preparation method thereof. A silver inorganic antimicrobial is characterized by high content of silver, safety and the like, and the silver inorganic antimicrobial which adopts zirconium phosphate as a carrier is selected and mixed into catheter material to prepare the sustained release type antibacterial medical catheter that is most widely applied to clinic, such as an antibacterial catheter, an antibacterial central venous catheter, an antibacterial trachea cannula and the like. The catheter has high use safety, wide antibacterial spectrum, good antibacterial effects, antimicrobial constituent sustained release and long duration of antibacterial effects, and can reduce the frequency of replacing medical catheters so as to have safe and convenient clinical use, effectively reduce nosocomial infections related to catheters due to the use of the medical catheters, greatly reduce medical expenses related to anti-infection, and the like, and save medical resources.

The invention relates to a composite natural fresh-keeping agent for cold fresh meat and application of composite natural fresh-keeping agent. The composite natural fresh-keeping agent for the cold fresh meat is characterized by comprising tea polyphenol, chitosan, nisin, natamycin, lysozyme, oregano essential oil, vitamin C, vitamin E, erythorbic acid, a spice extract and water in certain proportions by weight, wherein the spice extract is obtained by extracting ginger, garlic, cinnamon, cardamom, clove and rosemary in certain proportions by weight through ethanol extraction. The composite natural fresh-keeping agent for the cold fresh meat is prepared from biological-source raw materials by a simple preparation method, has the advantages of non-toxicity, safety, broad antibacterial spectrum and the like, is convenient to operate during using and prominent in fresh-keeping effect, and has an important industrial value.

The invention discloses a preparing method of HaiNan speckle equal scorpion antibiotic peptide gene and appliance, which comprises the following steps: restructuring bacillus coli Escherichia coli DH5a/ImAMP1, CCTCC NO: M207034; constructing HaiNan speckle equal scorpion ioterium cell cDNA library; designing primer; choosing PCR method; sieving positive colony of scorpion antibiotic peptide gene from ioterium cDNA library; sequence-analyzing coding trait of antibiotic peptide gene; assuring amino acid sequence of the antibiotic peptide gene; adopting chemosynthesis antibiotic peptide; possessing inhibition of diverse density for Gram's bacterium. This peptide can be used to medicine to prevent and cure Gram's bacterium.

The invention relates to a biological-source nutritional preservative for seafood. The biological-source nutritional preservative is characterized in that each liter of the distilled water of the biological-source nutritional preservative contains 0.4-0.6g of lysozyme, 2.5-3.5g of tea polyphenols, 20-28g of carboxymethyl chitosan, 10-14g of nisin, 17-23g of sodium alginate, 20-30g of lactobacillus, 1.5-2.5g of propolis extract and 10-14g of spice extract, wherein the spice extract is made of rhizoma galangae, garlic, onion, cinnamon, clove and rosemary. The biological-source nutritional preservative has the advantages that the biological-source nutritional preservative is compound by biological preservatives and is safe, nontoxic, simple in preparation and application method, outstanding in microorganism inhibition effect, good in preservation effect, capable of greatly prolonging the shelf life of the seafood and high in industry value.

The invention relates to compound tylosin injection for animals and a preparation method thereof. The injection consists of tylosin, doxycycline hydrochloride, trimethoprim, aminophylline, dexamethasone sodium phosphate, organic solvent and injection water. As a dedicated compound preparation for animals, the injection has special effect for treating respiratory tract infection caused by livestock and poultry sensitive bacteria and mycoplasmosis of pigs and poultry. The method has the advantages of convenient use, short course of treatment, low drug resistance and the like.

The invention provides a microorganism ecology conditioner and application. The microorganism ecology conditioner is prepared by the following steps: placing a culture medium in a culture vessel, sterilizing, firstly inoculating bacillus megaterium weighing 4-10% of the culture medium at the culture temperature 26-35 DEG C, culturing for 36-48 hours, then inoculating bacillus subtilis weighing 3-8% of the culture medium, culturing for 48 hours again, then inoculating Trichoderma Vride G weighing 0.5-6% of the culture medium, finally culturing for 48-144 hours, afterwards adding calcium carbonate weighing 5-10% of the culture medium or clay weighing 5-15% of the culture medium as the carrier, adding adsorbent weighing 100-1000 times of the culture medium, mixing to be uniform, stacking and fermenting for 3-6 days, drying, and concentrating into solid or powdery finished product. The microorganism ecology conditioner can be taken as biological fertilizer having biological prevention and control functions, and can also be taken as soil conditioner and continuous cropping obstacle soil restoring agent.

The invention relates to a chitosan gel dropping pill for gynaecologic bacteriostasis and a preparation method thereof. The gel dropping pill comprises the following constituents: compound chitosan with different molecular weights and deacetylation degrees, gelatinizer, acidifier, sodium chloride, purified water and dropping pill matrix. The method comprises the steps of mixing the compound chitosan and aqueous solution of sodium chloride with certain concentration, so as to form suspension, then feeding a proper amount of gelatinizer to the suspension, adjusting the pH value of the suspension through the acidifier, feeding compound chitosan gel obtained into matrix melt, uniformly mixing the compound chitosan gel and the matrix melt, and feeding the compound chitosan gel and the matrix melt into a pill dropping machine to prepare dropping pills, so as to obtain the chitosan gel dropping pill. The chitosan gel dropping pill is good in roundness, rapid in drug dissolution and high in bioavailability, the antibacterial ability of the chitosan gel dropping pill for gram-positive bacteria and gram-negative bacteria reaches greater than 99 percent, and the chitosan gel dropping pill is applied to the curing of monilial vaginitis, bacterial vaginitis, trichomonas vaginitis or cervicitis in gynecology, has good curative effect, high safety and good biocompatibility. The invention also relates to a preparation method and the medical application of the chitosan gel dropping pill.

The invention discloses pleuromutilin derivatives with the structure as shown in a formula (I) in the specification and the antibacterial activity, salts, solvates, optical isomers and polymorphism compounds thereof acceptable in pharmacy and/or veterinary medicines as well as a preparation method and an application of the pleuromutilin derivatives and the salts, solvates, optical isomers and polymorphism compounds thereof in antibacterial compositions. The preparation method has the beneficial effects that two different lead compounds are connected together by covalent bonds so as to generate a synergistic effect, an additive effect or new pharmacological activity in vivo; sulfonamides are spliced into side chains of pleuromutilin so as to generate new pleuromutilin derivatives, the antibacterial activity of the obtained new compounds can be enhanced, the obtained new compounds have obvious antibacterial activity for gram-positive bacterium, gram-negative bacterium and mycoplasma and an obvious inhibiting effect on drug-resistance bacteria such as MRSA, and the antibacterial spectrums of the obtained new compounds are effectively expanded.

The invention discloses a method for improving antibacterial performance of porcine lysozyme through N-terminal fusion with poly-hexapeptide and belongs to the field of bioengineering. The method comprises steps as follows: design of a fusion porcine lysozyme gene, expression by an escherichia coli expression system as well as renaturation. Antimicrobial activity detection proves that the fusion porcine lysozyme not only has obvious resistance to Gram-positive bacteria, but also can kill various Gram-negative bacteria, and a good case is provided for improving the antibacterial activity of lysozyme. The preparation technology for the porcine lysozyme has the characteristics of being simple and efficient, a product can be electrophoretically pure, and the purity is 90% or higher.

The invention relates to the field of drug synthesis and preparation thereof, relates to a preparation method of an antibacterial drug, in particular to a stable cefodizime sodium composition preparation and a preparation method thereof. The present invention directly dissolves sterile cefodizime sodium in water, adds sterile potassium clavulanate to dissolve it completely, and obtains an aqueous solution of cefodizime sodium/clavulanate potassium, and adds hydroxypropyl-β to the aqueous solution. -Cyclodextrin (HP-β-CD) inclusion, sub-package, and freeze-drying to obtain Cefodizime Sodium/Clavulanate Potassium for Injection. The preparation method provided by the invention is simple, and the cefodizime sodium/clavulanic acid potassium salt is clathrated with hydroxypropyl-β-cyclodextrin, which increases the stability of the sterile cefodizime sodium drug and reduces its toxic and side effects , improve drug availability, and the preparation process is simple, suitable for industrial production.

The invention discloses a mildew preventive which takes tebuconazole and 4, 5-dicholo-2-n-octyl-3-isothiazolinone as active components. The invention further discloses an application of the mildew preventive. As tebuconazole is relatively low in price while 4, 5-dicholo-2-n-octyl-3-isothiazolinone is relatively high in price, the tebuconazole and 4, 5-dicholo-2-n-octyl-3-isothiazolinone are compounded to obtain the mildew preventive, so that the cost is lowered while the mildew preventing effect is ensured. After the tebuconazole and 4, 5-dicholo-2-n-octyl-3-isothiazolinone are compounded, the antibacterial spectrum of the mildew preventive is expanded. The tebuconazole and 4, 5-dicholo-2-n-octyl-3-isothiazolinone are compounded, so that the mildew preventive has an obvious synergetic effect on the antibacterial effect of a lot of mildews.

The invention provides an antibacterial composition. The antibacterial composition is prepared by compounding essential plant oil with medium-chain fatty acid; the essential plant oil comprises carvacrol, cinnamaldehyde, and eugenol; and the medium-chain fatty acid comprises octanoic acid, decanoic acid, and caproic acid. The antibacterial composition provided by the invention overcomes problems of single use of organic acid or essential plant oil, and thus, the antibacterial composition is good in bacteriostatic effects, small in application dosage, and capable of effectively utilizing existing resources so as to reduce cost; moreover, the antibacterial composition has expanded antimicrobial spectrum, and can be used for preparing feed additives, such as acidifying agents and so on. The antibacterial composition is a safe product which can be used to replace antibiotics, and thus, the antibacterial composition has vast application prospects in the field of feeds.

The invention discloses a compound florfenicol preparation for aquatic products and a preparation method and application thereof. The compound florfenicol preparation for the aquatic products consists of the following components in percentage by mass: 5 to 20 percent of florfenicol, 1 to 25 percent of neomycin sulfate and the balance of glucose. The florfenicol and the neomycin sulfate are combined, so that the antibacterial effect of the compound florfenicol preparation for the aquatic products can be improved, the prevention and treatment effects of the compound florfenicol preparation on the bacterial diseases of the aquatic products are effectively improved, and medicine consumption and cost are reduced; and the preparation method of the compound florfenicol preparation for the aquatic products is simple and practicable and greatly improves bioavailability, and the preparation has high solubility.

The invention discloses a medical composite for treating livestock and poultry intestinal canal diseases and a preparation method and an application thereof, aiming at providing a medical composite and the preparation method and the application thereof, which can fast and effectively prevent and cure intestinal canal diseases. The composite comprises the following components in weight percent, 2%to 5% of pirquinozol antibiotics, 3% to 10% of beta-lactam antibiotics, 0.6% to 2% of beta-lactam inhibitor, 3% to 10% of aminoglycoside antibiotics and the balance auxiliary material acceptable in pharmacy. The pirquinozol antibiotics, such as norfloxacin lactate, is broad-spectrum antibiotics; the beta-lactam inhibitor has the functions of inhibiting enzyme and strengthening the effect; and thebeta-lactam antibiotics can destroy bacterial cell walls, which is favorable for aminoglycoside antibiotics to enter cells to take effect, increases the antibacterial effect and simultaneously enlarges the antibacterial spectrum. The composite of the invention can effectively cure canal diseases caused by Gram negative bacteria or anaerobe and the mixed infection of the Gram negative bacteria andthe anaerobe.

Antimicrobial peptide LF-TH and its Escherichia coli recombinant preparation method. It solves the problem of low antibacterial activity of the existing antibacterial peptides. The amino acid sequence of the antimicrobial peptide LF-TH includes 33 amino acids, its amino acid sequence is shown in SEQ ID No.1, and its coding gene sequence is shown in SEQ ID No.2. Methods: 1. Design the antimicrobial peptide LF-TH and the coding gene of the antimicrobial peptide LF-TH; 2. Link the coding gene of the antimicrobial peptide LF-TH to the plasmid and construct a recombinant Escherichia coli expression vector, transform E.coliBL21(DE3), and The fusion protein is obtained after induced expression and purification, the fusion protein is cleaved by enterokinase to release the antimicrobial peptide LF-TH, and the antimicrobial peptide LF-TH is obtained after purification. The antibacterial peptide LF-TH of the present invention has high antibacterial activity, and has obvious inhibitory effect on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium.

The invention relates to a preparation method for liquid egg with long preservation period, relating to the application of lysozyme, in particular to a producing method of the liquid egg. The invention solves the problem that the preservation period of the liquid egg after processing is short. The method of the invention is characterized in that: the lysozyme and synergistic substances are added into egg liquid, then pasteurized to obtain the liquid egg product; wherein, the additive amount of the lysozyme is 0.03 to 0.25 percent of the total weight of the liquid egg and the additive amount of the synergistic substances is 0.1 to 1.0 percent of the total weight of the liquid egg; the vigor of the lysozyme is 8000U/mg to 20000U/mg. Under the cold storage condition at the temperature of 0 to 4 DEG C, the preservation period of the liquid egg prepared by the invention reaches 6 weeks which is 3 weeks longer than the original preservation period.

The invention discloses a nosiheptide-colistin sulfate premixed agent which comprises 0.5-4 portions of nosiheptide, 1-10 portions of colistin, and the carriers of calcium carbonate, zeolite powder, corn flour, etc. for the rest in every 100 weight portions. The preparation method is as following. The raw materials are added in a mixing machine by the sequence of the carriers, the colistin sulfate, the nosiheptide and the carriers, and mixed evenly to make into powdery nosiheptide-colistin sulfate premixed agent. Or the nosiheptide and the carriers can be mixed fully firstly, then the colistin sulfate is dissolved in the water and sprinkled evenly on the mixture of the nosiheptide and the carriers. After being granulated, dried and screened, the mixture is made into the granular-typed nosiheptide-colistin sulfate premixed agent, the granularity of which is 30-60 mesh. The nosiheptide-colistin sulfate premixed agent of the invention is added in feedstuffs. The content range of the nosiheptide in every kilogram of overhead price feedstuff is 0.5-20mg, and the content range of the colistin sulfate in every kilogram of overhead price feedstuff is 2-40mg.