Biological deodorant spray for pets as well as preparation method and application of biological deodorant spray
A deodorant spray and biological deodorization technology, applied in the directions of deodorization, separation methods, and cosmetic preparations, etc., can solve the problems of pets being unable to judge disinfectants, inappropriateness, etc., and achieve the effect of removing odor and reducing odor.
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preparation example Construction
[0029] (1) Preparation of fermentation broth
[0030] 1. Turn on the ultraviolet light of the sterile room and ultra-clean workbench for 30-60 minutes 3 hours before the operation, turn off the ultraviolet light, close the door and wait for 1-2 hours before entering.
[0031] 2 Culture medium preparation:
[0032] Solid medium: LB agar medium, MRS agar medium and YPD agar medium are sterilized at 121°C for 20 minutes;
[0033] LB agar medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 15g, distilled 1000mL, pH7.0;
[0034] MRS agar medium: tryptone 10g, beef powder 5g, yeast powder 4g, glucose 20g, Tween 80 1mL, dimethyl hydrogen phosphate heptahydrate 2g, sodium acetate trihydrate 5g, triammonium citrate 2g, magnesium sulfate heptahydrate 0.2 g, manganese sulfate tetrahydrate 0.05g, agar 15g, distilled water 1000mL, pH 6.2;
[0035] YPD agar medium: yeast extract 10g, peptone 20g, glucose 20g, agar 20g, distilled water 1000mL, natural pH.
[0036] Liquid med...
Embodiment 1-3
[0074] According to the following raw material proportioning, take each raw material in each embodiment group respectively
[0075]
[0076]
[0077] Each group was supplemented with sterile water to make up a total volume of 100ml of raw materials.
[0078] Wherein, in each embodiment group, each raw material used is all the same, and wherein, the number of living bacteria in each bacterial liquid is respectively: Bacillus subtilis 1.2~1.5×10 10 cfu / mL; Bacillus licheniformis: 1.2~1.4×10 10 cfu / mL; Bacillus coagulans: 0.8~1.0×10 10 cfu / mL; Lactobacillus acidophilus: 0.6~0.8×10 10 cfu / mL; Enterococcus faecalis: 1.0~1.2×10 10 cfu / mL; Saccharomyces cerevisiae: 0.1~0.3×10 10 cfu / mL;
[0079] In the yucca extract, the content of yucca and saponin is ≥10.5%; the concentration of the aloe extract solution is 2% (w / v, the same below); the concentration of the solution of the patchouli extract is 2%; 12%, the concentration of ginkgo biloba extract solution is 5%, and the c...
experiment example 1
[0087] 1 test material
[0088] The basic dog food is the "R-King" series of adult dog food provided by Foshan Remigao Animal Nutrition and Health Technology Co., Ltd.; the biological deodorant spray used in the test is the above examples 1-3 and comparative examples 1-3 product.
[0089] 2 test animals
[0090] The test dogs were 240 Labradors provided by Foshan Remigao Animal Nutrition and Health Care Technology Co., Ltd. Pet Purebred Breeding Farm. The health and weight were basically the same. The age ranged from 2 to 3 years old. head. On the basis of gender, they were randomly divided into control group and experimental group I-VII, with 15 male dogs and 15 female dogs in each group.
[0091] 3 Test Management
[0092] The test dogs in the control group and the test group were raised separately in two houses, and closed feeding management was adopted uniformly, with one dog in one house, and quantitative feeding (feeding amount was calculated according to 2% of body ...
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