Polypeptide and preparation method thereof

A host cell and nucleic acid sequence technology, applied in the field of polypeptides and their preparation, can solve the problems of low hemolysis rate, unsatisfactory antibacterial activity and stability of natural polypeptides, and high toxicity, and achieve low hemolysis, strong broad-spectrum antibacterial activity, The effect of low production cost
CN114031673APending Publication Date: 2022-02-11杭州长龄生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
杭州长龄生物科技有限公司
Publication Date
2022-02-11

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Abstract

The invention provides a polypeptide and a preparation method thereof, and relates to the technical field of biological pharmacy. Artificial design and modification are carried out on the basis of polypeptide from a natural source, the polypeptide shown in the amino acid sequence of SEQ ID NO.1-5 is provided, and the blood solubility of the polypeptide is reduced on the basis of keeping the biological activity of the polypeptide. The preparation method comprises the following steps: S1, artificially designing an amino acid sequence of the polypeptide and a nucleotide sequence for coding the amino acid sequence; S2, connecting the nucleotide sequence with an expression vector, transforming and/or transfecting into a host cell, and screening positive clones; S3, culturing positive clones, and inducing polypeptide expression; and S4, breaking the host cell, and purifying the protein polypeptide to obtain the polypeptide. The preparation method is high in expression efficiency, simple in separation and purification process and high in product purity, the purity of the obtained polypeptide can reach 98%, the pharmaceutical industrialization level is reached, the production cost is low, and the preparation method is suitable for large-scale industrial production.
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Description

technical field

[0001] The invention belongs to the technical field of biopharmaceuticals, and specifically relates to a polypeptide and a preparation method thereof. Background technique

[0002] With the widespread use of antibiotics, the phenomenon of abuse has generally increased, accompanied by the continuous increase of drug-resistant strains, making anti-infection treatment fall into a crisis of drug resistance. When synthetic quinolone antibacterial drugs came into the market, clinical pathogenic bacteria were very sensitive to this new type of antibacterial drug, and there were almost no drug-resistant strains. After years of extensive use, the resistance rate of clinical pathogenic bacteria to this type of synthetic antibacterial drug increased rapidly. For example, the resistance of Escherichia coli to quinolones is as high as 70%. In order to deal with drug-resistant bacterial infections, the existing technology is on the one hand structurally modifying traditio...

Claims

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