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Method for extracting nitrogen-containing compounds from Polyrhachis vicina Roger, and application of nitrogen-containing compounds

A nitrogen compound and extraction method technology, applied in the purification/separation of amino compounds, organic chemistry, carboxylic acid amide separation/purification, etc., to achieve good antibacterial activity and enhance the effect of medicinal value

Active Publication Date: 2016-11-09
东营悦来湖园区运营管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that there are a large number of valuable secondary metabolites and biologically active diversity substances in black ants, especially the antibacterial active ingredients contained in the body need to be further studied

Method used

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  • Method for extracting nitrogen-containing compounds from Polyrhachis vicina Roger, and application of nitrogen-containing compounds
  • Method for extracting nitrogen-containing compounds from Polyrhachis vicina Roger, and application of nitrogen-containing compounds
  • Method for extracting nitrogen-containing compounds from Polyrhachis vicina Roger, and application of nitrogen-containing compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] A method for extracting nitrogenous compounds in black ants, comprising the following steps:

[0063] Step 1. Take 1.5 kg of black ant raw material, clean the surface mud stains and remove inorganic salts for storage and treatment, clean it, put it in a 45°C oven and blow dry it to constant weight, crush it to get black ant powder, and set it aside;

[0064] Step 2. Soak the black ant powder obtained in step 1 with 5 times the weight of dichloromethane solvent at room temperature, filter after 48 hours, filter out the black ant residue, add dichloromethane 5 times the weight of the black ant residue to repeat the extraction , filter, collect filtrate and filter residue respectively, filter residue is through drying, obtain black ant filter residue powder, standby;

[0065] Step 3, place the black ant residue powder obtained in step 2 to dry in a ventilated place, add a methanol solution with a mass percentage of 60%, extract by cold soaking at room temperature for 48h, ...

Embodiment 2

[0075] A method for extracting nitrogenous compounds in black ants, comprising the following steps:

[0076] Step 1. Take 2.5 kg of black ant raw material, clean the surface mud stains and remove the inorganic salts for storage and treatment, after cleaning, put it in a 48°C oven and blow dry it to constant weight, crush it to get black ant powder, and set it aside;

[0077] Step 2. Soak the black ant powder obtained in step 1 with dichloromethane solvent 7 times its weight at room temperature, filter after 49 hours, filter out the black ant residue and add dichloromethane 7 times the weight of the black ant residue to repeat the extraction , filter, collect filtrate and filter residue respectively, filter residue is through drying, obtain black ant filter residue powder, standby;

[0078] Step 3, place the black ant residue powder obtained in step 2 in a ventilated place to dry, add a methanol solution with a mass percentage of 70%, cold soak at room temperature and extract f...

Embodiment 3

[0088] A method for extracting nitrogenous compounds in black ants, comprising the following steps:

[0089] Step 1. Take 3.0 kg of black ant raw material, clean the surface mud stains and remove inorganic salts for storage and treatment, after cleaning, put it in a 50°C oven and blow dry it to constant weight, crush it to obtain black ant powder, and set it aside;

[0090] Step 2. Soak the black ant powder obtained in step 1 with dichloromethane solvent 10 times its weight at room temperature, filter after 50 hours, filter out the black ant residue, add dichloromethane 10 times the weight of the black ant residue to repeat extraction , filter, collect filtrate and filter residue respectively, filter residue is through drying, obtain black ant filter residue powder, standby;

[0091] Step 3, place the black ant residue powder obtained in step 2 to air-dry in a ventilated place, add a methanol solution of 80% by mass percentage and extract by cold soaking at room temperature fo...

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Abstract

The invention discloses a method for extracting nitrogen-containing compounds from Polyrhachis vicina Roger, and an application of the nitrogen-containing compounds. The method comprises the following steps: carrying out room-temperature leaching extraction on Polyrhachis vicina Roger used as a raw material by using a dichloromethane solvent to obtain Polyrhachis vicina Roger filter residues, carrying out room-temperature leaching extraction on the Polyrhachis vicina Roger filter residues by using a methanol solution with the mass percentage of 60-80%, freeze-drying a methanol concentrate obtained after extraction to obtain methanol extract, sequentially eluting the methanol extract with an ethyl acetate solution and an ethyl acetate-methanol mixed solution, and further separating and purifying obtained fraction to finally extract six nitrogen-containing compounds. The method allows the antibacterial nitrogen-containing compounds rich in the Polyrhachis vicina Roger to be highly enriched or extracted as possible, and the extracted and separated nitrogen-containing compounds have good antibacterial activity, and further provide lead compounds for research and development of antibacterial new drugs.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and in particular relates to a method for extracting nitrogen-containing compounds from black ants and an application of the nitrogen-containing compounds. Background technique [0002] The living environment infested by breeding insects, just like super bacteria, is naturally able to avoid the attack of any invading bacterial infection, presents a special antibacterial survival ability, and has a strong ability to defend against microorganisms and bacteria. This biological characteristic is We search for new antibacterial natural compounds that have the potential to address the current problem of antibiotic resistance. The black ant (Polyrhachzs vzczna Roger), also known as the pseudo-black spiny ant, is an insect belonging to the phylum Arthropoda, the class Insecta, the order Anoptera, and the genus Polyrhachzs of the family Formicaceae. It is a very ancient social insect. In recent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C233/18C07C231/24C07D207/36C07D253/06C07C211/38C07C209/86C07D233/88A61P31/04
CPCC07C209/86C07C231/24C07D207/36C07D233/88C07D253/06C07C233/18C07C211/38Y02A50/30
Inventor 尹卫平李海迪高嘉屿刘军娜刘华清
Owner 东营悦来湖园区运营管理有限公司
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