A kind of alginic acid-chitooligosaccharide-zinc oxide composite hydrogel and its preparation method and application

A composite hydrogel and chitosan oligosaccharide technology, which can be used in pharmaceutical formulations, bandages, drug delivery, etc., can solve the problems of low antibacterial activity, improvement of wound healing effect, poor mechanical properties and biocompatibility, and achieve good results. Wound repairing effect, overcoming weak mechanical properties, and strong broad-spectrum antibacterial activity

Active Publication Date: 2022-04-29
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the deficiencies of existing hydrogel dressings, such as low antibacterial activity, poor mechanical properties and biocompatibility, and the effect of promoting wound healing needs to be improved, the present invention discloses a composite hydrogel dressing of alginic acid-chitooligosaccharide-zinc oxide Glue, which can simultaneously exert the hydrophilic and water-retaining properties of alginic acid and the antibacterial effect of chitosan and nano-zinc oxide, and has good mechanical properties, has broad market application prospects in wound healing dressings and other biomedical materials

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  • A kind of alginic acid-chitooligosaccharide-zinc oxide composite hydrogel and its preparation method and application
  • A kind of alginic acid-chitooligosaccharide-zinc oxide composite hydrogel and its preparation method and application
  • A kind of alginic acid-chitooligosaccharide-zinc oxide composite hydrogel and its preparation method and application

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Embodiment 1

[0046] A preparation method of alginic acid-chitooligosaccharide hydrogel is as follows: Weigh 20 g of alginic acid (viscosity 10 mPa s) and disperse it in 75 mL of ethanol, then slowly drop into the reaction mixture 100 g of periodate containing 2.7 g mL of aqueous solution was stirred at room temperature and protected from light for 48 h. After the reaction was completed, ethylene glycol was added to continue stirring for 0.5 h to terminate the reaction. After precipitation with 4 volumes of 95% ethanol, dialysis and freeze-drying, oxidized alginic acid was obtained. Mix 0.5 mL of 200 mg / mL oxidized alginic acid solution with 0.5 mL of 150 mg / mL chitosan oligosaccharide (3 kDa) solution, stir and mix well to obtain alginic acid-chitooligosaccharide hydrogel.

[0047] The hydrogel includes the following components in mass percentage: 20% oxidized alginic acid, 15% chitosan oligosaccharide, and the balance is water.

Embodiment 2

[0049] A preparation method of alginic acid-chitooligosaccharide-zinc oxide composite hydrogel is as follows: Weigh 20 g of alginic acid (viscosity 50 mPa s) and disperse it in 75 mL of ethanol, and then slowly drop into the dispersion system 10.8 g of acid in 100 mL of aqueous solution was stirred at room temperature and protected from light for 24 h. After the reaction was completed, ethylene glycol was added and stirred for 1 h to terminate the reaction. After precipitation with 4 volumes of 95% ethanol, dialysis and freeze-drying, oxidized alginic acid was obtained. Drop 0.1 mol / L sodium hydroxide solution (solvent is methanol) into 0.1 mol / L zinc acetate solution (solvent is methanol), stir continuously for 4 h, remove by-products by centrifugation and washing, and calcinate the precipitate at above 300°C, Zinc oxide nanoparticles are obtained. Disperse 500 μg of zinc oxide nanoparticles in 0.5 mL of 100 mg / mL oxidized alginic acid solution to obtain a solution with a co...

Embodiment 3

[0052] A preparation method of alginic acid-chitooligosaccharide-zinc oxide composite hydrogel is as follows: Weigh 20 g of alginic acid (viscosity 80 mPa s) and disperse it in 75 mL of ethanol, and then slowly drop into the dispersion system 21.6 g of potassium potassium chloride in 100 mL of aqueous solution was stirred at room temperature and protected from light for 2 h. After the reaction was completed, ethylene glycol was added to continue stirring for 3 h to terminate the reaction. After precipitation with 4 volumes of 95% ethanol, dialysis and freeze-drying, oxidized alginic acid was obtained. Drop 0.1 mol / L ammonium hydroxide solution (solvent is methanol) into 0.1 mol / L zinc sulfate solution (solvent is methanol), stir continuously for 6 h, remove by-products by centrifugation and washing, and deposit the precipitate at 300°C or higher calcined in a furnace to obtain zinc oxide nanoparticles. Disperse 250 μg of zinc oxide nanoparticles in 0.5 mL of 100 mg / mL oxidize...

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Abstract

The invention provides an alginic acid-chitooligosaccharide-zinc oxide composite hydrogel and its preparation method and application. The present invention uses alginic acid as a raw material, prepares oxidized alginic acid through an oxidant, then cross-links the aldehyde group after alginic acid oxidation with the amino group of chitosan oligosaccharide through Schiff base, and simultaneously loads zinc oxide nanoparticles to prepare alginic acid- Oligochitosan‑Zinc Oxide Composite Hydrogel. It is verified by experimental research that the composite hydrogel of the present invention has a porous three-dimensional structure, good mechanical properties, and realizes Zn 2+ slow release. The alginic acid-chitooligosaccharide-zinc oxide composite hydrogel not only has hydrophilicity, water retention and biocompatibility, but also has broad-spectrum antibacterial effect and can effectively promote wound healing. Therefore, the composite hydrogel shows obvious advantages in promoting wound healing, antibacterial infection and water retention, and has broad application prospects in tissue engineering applications.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an alginic acid-chitooligosaccharide-zinc oxide composite hydrogel and a preparation method and application thereof. Background technique [0002] The skin is the largest organ in the human body and plays an important role in protecting internal organs from external factors such as external forces, ultraviolet rays, and microorganisms. Loss of skin integrity due to injury or surgery is prone to microbial infection, and controlling wound infection and promoting wound healing are important clinical concerns. According to the theory of moist wound healing, keeping the wound moist, breathable and antibacterial microenvironment can promote the proliferation and migration of epidermal cells, which is beneficial to wound healing and inhibits scar formation. Hydrogel is a three-dimensional network formed by the cross-linking of hydrophilic polymer chains, which has chara...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L5/04C08L5/08C08K3/22A61L26/00
CPCC08J3/075A61L26/008A61L26/0066A61L26/0061A61L26/0023A61L26/0004C08J2305/04C08J2405/08C08J2305/08C08J2405/04C08K2003/2296C08K2201/011A61L2300/102A61L2300/404A61L2300/602A61L2400/12C08L5/04C08L5/08
Inventor 赵峡张苗陈祥艳蒋天泽宋盼盼
Owner OCEAN UNIV OF CHINA
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