42 results about "Gram Positive Bacillus" patented technology

The invention provides a marine Streptomyces strain and use thereof. The strain provided by the invention is Streptomyces sp. FXJ7.388, and the collection number of the strain is CGMCC No.4474. The invention also provides the use of the strain in the preparation of an antibacterial product. The antibacterial effect is to inhibit gram-positive bacteria, including Multi-resistant Staphylococcus aureus (MRSA 1-1), staphylococcus aureus CGMCC1.2386, bacillus subtilisGCMCC1.2428 and/or Candida albicans 2.538. Experiments prove that the Streptomyces sp. FXJ7.388 separated by the invention has the advantage of resisting gram-positive bacteria and Candida albicans, namely having high activity for resisting MRSA, staphylococcus aureus AND bacillus subtilis and certain inhibiting effect on Candida albicans.

The invention discloses an organic solvent fastness alkaline proteinase generation strain and the gene and an application for catalyzing peptide to synthesize and resolve racemic amine and amino acid in the organic phase. The bacterial categorization naming is Bacillus licheniformis YP1A whose preservation register number is CCTCC No: M 207021 as Gram-positive bacillus and can tolerate certain density of a plurality of organic solvents. The invention separates and clones to obtain the coding gene of the proteinase generation strain, which is provided with nucleic acid sequence which is represented as SEQ ID NO: 1 and amino acid sequence as SEQ ID NO: 2. The organic solvent fastness alkaline proteinase is high in specific activity, strong in solvent tolerance, wide in action pH scale, strong in thermostable and strong alkalinity tolerance and the like. The proteinase can be applied in the application such as peptide synthesis in the organic phase, racemic amine and amino acid resolution and the like.

A method of inhibiting the growth R of a Gram positive bacterium, the method comprising treating the bacterium with an effective amount of one or more furanones having the Formula as set out in the Figure, wherein the effective amount of the one or more furanones does not substantially adversely effect the survival of an animal cell when exposed to the one or more furanones.

The invention discloses a mutation breeding method of lactobacillus plantarum and application of a strain of the lactobacillus plantarum. The collection number of the lactobacillus plantarum in China general microbiological culture collection center is CGMCC No 5297. The lactobacillus plantarum can generate bacteriocin which has a better inhibiting effect on multiple gram-positive bacteria and especially has great a high inhibiting effect on listeria monocytogenes, and the bacteriocin has the advantages of better heat stability, acid stability and the like and can be applied as a food additive and a feed preservative agent which have wide application prospect.

The invention provides a novel spirulina polypeptide P1 with bacteriostatic activity. An amino acid sequence of the spirulina polypeptide P1 is shown as Seq ID No.1. The spirulina polypeptide P1 with bacteriostatic activity is extracted from spirulina for the first time. Experiments demonstrate that the spirulina polypeptide P1 can effectively suppress gram-positive bacteria and gram-negative bacteria, has no toxic or side effect, can be used as a broad-spectrum antibacterial agent or a bacteria preservative, and has wide application prospects.

The invention relates to a novel melittin variant and an application thereof. At current, overuse of novel antibacterials and antibiotics is clinically to be developed, melittin as a good biology antibacterial agent is one of the best substitutes of the antibiotics. The inventor finds novel melittin based on the research. The melittin has good bacteriostasis and sterilizing capacity for Gram-negative bacteria, and through sequence improvement, the variant is obtained. Based on reservation of Gram-negative bacteria resistance of wild type melittin, good Gram-negative bacteria resistance is obtained. The novel melittin and the variant thereof provided by the invention have favorable clinical application prospects.

The invention discloses Lactobacillus casei WX322 with a preservation number of CGMCC No.17710, a strain and its metabolites have broad-spectrum antibacterial activity, which can exhibit strong antibacterial ability against a variety of Gram-positive and Gram-negative bacteria (including pathogenic and spoilage bacteria), can be used to prepare antibacterial or/and antiseptic products, and can beused in the fields of medical, food and other industry to control bacterial pathogens or spoilage bacteria, can also effectively inhibit the growth of the soft rot pathogen Pectobacterium carotovorum,and can be used to prepare products for controlling bacterial soft rot of vegetables, can control the decay of vegetables caused by Pectobacterium carotovorum before and after harvest, can effectively solve the problem of agricultural residues and environmental pollution caused by pesticide control, has good application potential in the biological control of vegetable bacterial soft rot, and alsoprovides an excellent basic strain for the development of microbial pesticides.

The invention discloses a cellulose decomposing strain CX10 from a termite gut and application thereof. The strain is preserved in the General Microbiological Center of China Committee for Culture Collection of Microorganisms at the No.3, first yard, Beichen west road, Chaoyang district, Beijing, and the preservation number is CGMCC No.16344. The strain is a gram-positive bacillus and free of capsules, endogenous spores can be seen after spore staining, the bacterial colony is a single one and in a cracked shape, the bacterial colony is white, and the edge of the bacterial colony is complete.The strain is a facultative anaerobic strain for producing cellulase; within 48 hours, the FPA enzyme activity of the strain is 49.5 U/mL, the CMCase enzyme activity is 95.0 IU/mL, and the CX enzyme activity is 23.26 IU/mL. The strain is very high in biodiversity value, and has a scientific value of further research on cellulose-enriched crops and development and application of a traditional Chinese medicine fermentation recycling.

The invention provides polypeptide with resistance to gram-positive bacteria, and a gene for coding the polypeptide. The sequence of the polypeptide is as show in SEQ ID NO:1, and the nucleotide sequence of the gene is as shown in SEQ ID NO:2. Pomfret is infected by gram positive microorganisms frequently used in the field of aquatic products, the polypeptide with resistance to gram-positive bacteria is obtained by using a subtractive hybridization method, and the polypeptide can be used as a feed additive for the pomfret.

The invention relates to purification, gene cloning, and enzymatic characteristic identification of chitosanase. A strain is separated from a contaminated yeast culture solution, and is identified to be a gram-positive bacillus; a main protein product secreted by the strain is purified, and subjected to N-end amino acid sequence determination, and the result shows that the protein product is quite similar to some known endo-chitosanases. Based on the homologous DNA sequence, a full-length gene is cloned by a PCR method. The strain grows quite rapidly, and the constitutive secretion product is mainly endo-chitosanase. The enzyme can be purified by two steps of hydrophobic chromatography and molecular sieve. The enzymatic characteristics of the enzyme and the conditions for catalyzing the degradation of chitosan into chitosan oligosaccharide by the enzyme are further studied. When the yield of the chitosanase is increased, the chitosanase is expected to be used for chitosan oligosaccharide production, and the chitosanase has actual application value.

The invention discloses Lactobacillus plantarum MS031 with a deposit number of CGMCC No. 17711. The bacterium has broad-spectrum antibacterial activity and can further produce various antibacterial active metabolites, including bacteriocins capable of inhibiting Gram-positive bacteria and broad-spectrum antibacterial metabolites capable of inhibiting both Gram-positive bacteria and Gram-negative bacteria, and can be used for preparing antibacterial or/and antiseptic products. The broad-spectrum antibacterial metabolites are respectively adsorbed on surface of the bacteria and secreted into fermentation liquor, can effectively inhibit growth of various food-borne pathogenic bacteria such as Listeria monocytogenes, salmonella, escherichia coli and the like in fresh-cut fruits and vegetables,can make up for shortcomings that most existing antibacterial agents have no effect on Gram-negative bacteria, and have good application prospects.

Lactobacillus sake is a natural food preservative and feed additive with potential development capacity; the lactobacillus sake is simplex in obtaining method and small in yield, so use and popularization of the lactobacillus sake are seriously influenced; meanwhile the lactobacillus sake is complex in preparation step and high in expenditure. A method for obtaining the lactobacillus sake by separating, screening and identifying bacteriocin producing lactic acid bacteria includes the steps of firstly, primary screening work of strains; secondly, secondary screening work of the strains, wherein in the secondary screening of the strains, an antibacterial test is conducted on a plurality of lactic acid bacteria strains obtained in the first step with gram-positive bacteria and gram-negative bacteria as indicator bacteria, and the strains which can inhibit gram-positive bacteria and gram-negative bacteria and have the dual inhibition characteristic are selected; thirdly, fermentation characteristic test work; fourthly, inspection of influences of other factors on the strains; fifthly, inspection of influences of pH and temperature on bacteriocin crude extracts; sixthly, physiology and biochemistry identification work and molecule identification work.

The invention discloses a method and a reagent kit for establishing an experimental model of a diabetic foot ulcer rat with mixed infection of Gram positive bacteria and Gram negative bacteria. The method comprises the following steps: after the rat is fed by a high fat feed for eight weeks, injecting streptozotocin to generate a diabetic model, after the diabetic model is stable, generating a scald with the depth of II degrees in the skin of the diabetic rat, and three days later, injecting mixed suspension of the Gram positive bacteria and the Gram negative bacteria at the scald. In addition, the invention also discloses the reagent kit for establishing the experimental model of the diabetic foot ulcer rat with the mixed infection of the Gram positive bacteria and the Gram negative bacteria. The reagent kit comprises the mixed suspension of the Gram positive bacteria and the Gram negative bacteria. According to the diabetic ulcer experimental animal model disclosed by the invention, by detecting the changes of biochemical indicators after drug intervention, results show that the model has the characteristic of being capable of generating the diabetic foot ulcer with various bacteria colony infections, and the characteristics of long healing time of an ulcer wound surface, sensitivity to drug reaction and the like.

The invention discloses bacillus firmus CX8 from a termite gut. The strain is collected in the China General Microbiological Culture Collection Center (CGMCC) with the address of No.3, No.1 Yard, Beichen West Road, Beijing 3 Chaoyang District on August 27th, 2018, and has a collection number of CGMCC No. 16342. The strain is gram-positive bacillus and is free of capsule, endogenous spores can be seen through spore staining, a bacterial colony is round and single, the color of the bacterial colony is white, and the edge of the bacterial colony is complete. The strain is a facultative anaerobicstrain for producing cellulase; and within 48 hours, the CMCase enzyme activity of the strain is 67.8 IU/mL, and the CX enzyme activity of the strain is 20.96 IU/mL. The strain not only has very highbiodiversity value, but also has scientific value for further research in development and application of cellulose-enriched crop and traditional Chinese medicine fermentation recycling technologies.

The invention discloses a method for separating and identifying pathogenic bacteria of insects, relating to the field of research for bacterial diseases of insects. The method comprises the followingsteps: S1, disinfecting the body surfaces of the insects carrying pathogenic bacteria, extracting hemolymph, carrying out diluting till the suitable concentration is achieved, coating an LB plate withthe diluent, carrying out culturing, picking out single colonies, thus obtaining pathogenic bacteria, carrying out pure culturing, transferring to liquid LB culture medium, and observing the morphological features of isolated strains under an oil immersion lens; S2, treating the thallus obtained by culturing in S1 by using lysozyme and SDS in sequence, and after the thallus is crushed, purifyingthe total DNA of the isolated strains through phenol extraction; and S3, amplifying the 16S rDNA with overall length of the isolated strains, after sequencing, carrying out clustering analysis, and determining the species of the isolated strain in combination with the morphological features of an isolated strain. The method for separating and identifying pathogenic bacteria of insects is universalfor gram-positive bacteria and gram-negative bacteria, and is suitable for separating and identifying pathogenic bacteria of the insects including bees, cotton bollworms, inchworms, Bombyx mori and the like, and the screened 16S rDNA amplification and sequencing primers are good in universality and high in stability.