45results about How to "Promote Applied Research" patented technology

The invention discloses a building method of fish head and kidney tissue derived cell lines and the obtained cell lines. The cell lines are obtained by performing a primary culture and a subculture for the treated fish heads and kidney tissues, wherein an employed cell culture medium is prepared by adding fetal calf serum, sodium pyruvate, 2-mercaptoethanol and a human basic fibroblast growth factor into a basic cell culture medium.

The invention provides a heavy immunodeficiency pig model and a construction method and application thereof. The method utilizes a BE3 point mutation tool for knocking out RAG1, RAG2 and IL2RG genes to obtain an immunodeficiency models; due to the C mutation T point mutation tool, the multi-gene knockout immunodeficiency model is obtained, the safety is better, the immunodeficiency degree is higher, T cells, B cells and NK cells are completely lost, follow-up application research is facilitated, the construction method is simple and efficiency, and the wide application prospect and huge marketvalue are achieved.

The present invention discloses preparation process of composite onion-shaped fullerene material with homogeneous grain size. Through chemical vapor deposition with zeolite of different types and homogeneous pore size as template, added catalyst and cheap inert gas and nitrogen as carrier gas, and in effectively controlled technological parameters, including reaction temperature, time, gas flow rate, etc. composite onion-shaped fullerene material in homogeneous grain size is prepared. The technological process of the present invention is simple and fast, and the prepared onion-shaped fullerene has high purity, stable performance and other advantages and great latent application value.

The invention provides an integrated single-fiber droplet manipulator. The integrated single-fiber droplet manipulator is characterized in that the device is composed of a section of multi-core optical fiber comprising a cladding and a fiber core, the fiber end is ground to form a cone frustum fiber end, the end face of the cone frustum fiber end is provided with a layer of liquid injection smoothporous surface film doped with a photosensitive material, and the film is provided with an array groove structure filled with a lubricating agent; when light waves transmitted by the fiber core are reflected by the cone frustum fiber end and then gathered to the liquid injection smooth porous surface film on the end face, the light waves are absorbed and converted into heat energy by the photosensitive material, a temperature gradient field is formed in an irradiation area, droplets attached to the liquid injection smooth porous surface film generate wetting gradient force under the influenceof the temperature gradient field, the droplets are affected by force, the posture of the droplets changes to generatesdirectional sliding, and finally, the droplets are controlled under the action of different temperature gradient fields by controlling the power of the light injected into the fiber core. The manipulator can be applied to the fields of hydromechanics, biology, medicine and the like.

The poly metal acid salt compound has molecular formula as Na14[MoVI126MoV28O462H14(H2O)68]íñca.300H2O, belongs to orthorhombic crystal system with space group as Cmca and single cell parameters as aú¢50.6844(4)ú¼bú¢56.3056(1)(cú¢30.6337(1)ú¼Vú¢87422.9(8)(3)ú¼Zú¢4. This product uses elementary huge-wheel ring shape as basic unit and Oxygen Bridge to link into two-dimension layer structure of calandria form, which can be used in adsorption material or solid fuel cell.

The invention discloses a method for detecting and counting live microbes, comprising the following steps of: carrying out live bacteria cultivation and counting on a sample to be detected, and initially screening positive suspected microbes; (2) randomly selecting the positive suspected microbes obtained from the step (1), extracting genome DNA, carrying out PCR (Polymerase Chain Reaction) detection by using a specific primer of a target microbe, and counting. The invention organically combines a PCR technology with the traditional microbe counting technology, has high specificity and sensitivity and good reliability, can distinguish the live microbes and dead microbes which are contained in the sample and has little result error; and in addition, the invention has the advantages of short operating time, simple steps, nontoxic and innocuous reagent and high actual popularization and application prospect, is suitable for probiotics counting in mixed cultivation and planting counting research on probiotics in intestinal canals and is particularly suitable for accurate counting when disturbing microbes contained in the sample and target microbes to be detected are similar.

The invention discloses an infrared two-photon coincidence measurement device and method based on a periodically poled lithium niobate waveguide, and the device comprises an infrared laser device, the laser emitted by the infrared laser device sequentially passes through an optical fiber attenuator and a polarizer and then enters an optical fiber beam splitter, and the incident light is divided into two light beams; one beam of light is received by a single-photon detector after being subjected to two-photon up-conversion through a PPLN waveguide to generate visible light; and the other beam of light is received by a light intensity detector for calculating the light intensity entering the PPLN waveguide, and the infrared two-photon up-conversion efficiency of the corresponding two-photon coincidence measurement device is obtained by comparing the counting rate of the single-photon detector with the detection dependency relationship of the light intensity detector. The infrared two-photon coincidence detector overcomes the defects that an existing infrared single-photon detector is low in efficiency and large in noise, infrared two-photon coincidence measurement can be achieved more efficiently, and second-order interference experiments and application research of infrared photons are facilitated.

The invention relates to an electric conduction composite material with a poly-2, 3-dimethylaniline / polyaniline core shell structure, and a preparation method of the electric conduction composite material. In the composite material, the poly-2, 3-dimethylaniline is used as a core, and the polyaniline is used as a shell. The preparation method comprises the following steps: the poly-2,3-dimethylaniline is firstly prepared by a solution polymerization process, and then is filtered, washed, dried and ground to obtain the powdered poly-2,3-dimethylaniline; and the powered poly-2, 3-dimethylaniline is used as the core, polymerization is carried out to the surface of the powered poly-2, 3-dimethylaniline by the solution polymerization process or emulsion polymerization process, and then a product is filtered, washed, dried and ground to obtain the electric conduction composite material with the poly-2, 3-dimethylaniline / polyaniline core shell structure. In the electric conduction composite material with the poly-2, 3-dimethylaniline / polyaniline core shell structure, the electric conductivity of the poly-2, 3-dimethylaniline / polyaniline is remarkably improved, thereby being beneficial tobeing applied to the field of sensors, electric conductive devices, electrode active materials and the like.

The invention discloses a culturing method for maintaining a self-renewal state of human embryonic stem cells. The method comprises passage and culturing of the human embryonic stem cells under the condition of using the culturing method and observation and detection of the self-renewal state of the human embryonic stem cells cultured under the condition of using the culturing method. According tothe method, the problems of easily differentiated state, complicated passage operation, high culturing condition cost and inconvenient subsequent research on mechanisms of the human embryonic stem cells under the current traditional culturing condition are solved by adding an inhibitor of a Wnt / beta-catenin signal channel; the method has the advantages of being stable in effect, economic and high-efficient, simple to operate, convenient to research and the like, clues for improving and establishing culturing conditions for multipotential stem cells of other kinds and species are provided, and the method has a positive promotion effect on smooth development of stem cell basis and application research in the future.

The invention discloses a vehicle trajectory clustering method based on a parallel ST-AGNES algorithm, and the method is characterized in that multiple processing units are utilized to work together,and a certain task is executed at the same time to achieve higher performance. Parallel computing is an effective means for improving the computing speed and the processing capacity of a computer system. Therefore, the invention provides the vehicle trajectory clustering method based on the parallel ST-AGNES algorithm from a brand-new perspective, and the vehicle trajectory time and space similarity measurement method based on dynamic time warping better solves the problems of different vehicle trajectory sampling rates and inconsistent time scales. The method comprises the steps: constructinga time and space similarity matrix of vehicle tracks; studying a splitting scheme of irregular trajectory data and a communication mechanism of parallel computing, wherein one of the object-orientedtrajectory data splitting schemes is an object-oriented trajectory data splitting scheme; carrying out clustering connection based on the minimum distance, and designing a parallel STAGNES algorithm oriented to vehicle trajectory data.

The invention relates to a human placenta chorion mesenchymal stem cell culture system which includes the steps: treating human placenta chorion tissues, performing primary culture and replacing a culture medium 7 days after; centrifuging tissue blocks, culture solution and flushing fluids in a primary culture bottle, performing renewal vaccination and secondary culture, and replacing the culture medium 7 days after; centrifuging tissue blocks, culture solution and flushing fluids in a secondary culture bottle, performing renewal vaccination and third culture, and replacing the culture medium 7 days after; performing digestive passage by pancreatin after the fusion degree of cells cultured for three times reaches 80-90% to obtain human placenta chorion mesenchymal stem cells. The system is simple and convenient to operate, and the number of the P0-generation human placenta chorion mesenchymal stem cells obtained by culture for three times doubles that of cells in primary culture.

The invention discloses a deep learning image denoising method and device for an infrared thermal imaging system. The method comprises the steps: obtaining a target object image collected by the infrared thermal imaging system, carrying out down-sampling processing, and segmenting the target object image into sub-images; performing image block extraction and pixel recombination on each sub-image to obtain a pre-processed sub-image; constructing a noise map, and adding the noise map as an additional channel to the preprocessed sub-image as an input image; taking the input image as a trained deep learning model, and outputting an estimated value of noise; and removing the estimated value of the noise from the target object image to obtain a denoised image. According to the method, rich structural features and similarity of the image can be fully utilized, context information in image block features can be better utilized, image features are fully extracted, a better effect is achieved under the condition that feature values of the infrared image are few, and application research of infrared thermal imaging and the deep learning technology is facilitated.

The invention provides a preparation method of a molybdenum disulfide pattern. The preparation method comprises the following steps: cutting a Mo foil pattern with a proper size; oxidizing the Mo foil pattern by using an electrochemical anodic oxidation method; and growing the MoS2 pattern by adopting an improved chemical vapor deposition method. The method for preparing the MoS2 patterns has the advantages of simplicity and convenience in operation, high practicability, diversified patterns, flexible position, capability of preparing large-area thin-layer uniform MoS2 film patterns and the like.

The invention provides a method and system for improving the adversarial defense capability of a Bayesian neural network, and relates to the technical field of Bayesian neural networks in the deep learning direction and adversarial defense, and the method comprises the following steps: a network training step: inputting initial data, and optimizing a cross entropy loss function and the KL divergence between parameter distribution and prior distribution to obtain a network model; training the initial data; and a robustness enhancement step: taking the parameters of the Bayesian neural network as input, calculating and optimizing the mathematical expectation of the spectral norm of the network parameter matrix, and reducing the sensitivity of the network to various noises according to the optimized mathematical expectation, thereby improving the adversarial defense capability of the Bayesian neural network model to the noises. According to the invention, the adversarial robustness of the Bayesian network model can be effectively improved, and the application prospect of the network model in practical problems is effectively enhanced; the output uncertainty of the network model is effectively reduced, the prediction of the network to the adversarial sample is more consistent, and the noise resistance of the network model is improved.

The invention provides a method for preparing a two-dimensional porous nano material based on diphenylacetylene molecule self-assembly. The method aims to prepare the two-dimensional nano material with a uniform porous structure on the surface of the single crystal by utilizing diphenylacetylene molecule self-assembly under an ultrahigh vacuum condition. The method comprises the following steps that 1, argon ion sputtering is carried out on a metal single crystal substrate, then annealing is carried out, and the sputtering and annealing processes are repeated; and 2, the metal single crystal treated in the step 1 is placed in an ultrahigh vacuum preparation cavity and heated to 50-53 DEG C, then diphenylacetylene molecules are deposited on the surface of the metal single crystal under the room temperature condition, the deposition time is 3-5 s, and the two-dimensional porous nanometer material is prepared. The method is simple in process, and the nanometer material with the uniform porous structure can be obtained. The pore size is 3.37 nm to 3.46 nm, the thickness of the pore wall is 0.7 nm to 1.2 nm, the height is 115 pm to 125 pm, and the coverage rate can reach 18% to 30%.

The invention discloses an infrared and visible light fusion imaging method based on deep learning. The method comprises the steps: collecting an infrared image and a visible light image of the same size for a target at the same time to form a target object image pair, carrying out the sub-image pair segmentation and preprocessing of sub-images in the target object image pair, and adding a noise image, inputting the sub-image pair added with the noise map into a trained image fusion model as an input image pair to obtain a fused image, wherein the image fusion model comprises a shallow feature extraction unit, an encoder, a fusion module and a decoder. The general fact that the source image contains noise is considered, the source image is used as the premise of actual imaging application, image denoising is performed in the image fusion process, and noise points in the source image pair are effectively prevented from being introduced into the fusion image; the method is very beneficial to application research of infrared and visible light fusion and a deep learning technology, and is expected to be widely applied to the fields of medical imaging, night monitoring and the like.

The invention provides a method for increasing the concentration of monodispersed carbon nanotube dispersion liquid, said method comprising the following steps: 1) preparing the initial dispersion liquid of carbon nanotube material and surfactant solution; 2) obtaining step 1) centrifuge the initial dispersion of carbon nanotubes, and collect the upper dispersion; 3) disperse the upper dispersion obtained in step 2); 4) centrifuge the dispersion obtained in step 3), and take the upper dispersion to obtain the final product. The present invention removes impurity components and hard-to-disperse carbon nanotube aggregates in the dispersion liquid through dispersion-centrifugation-redispersion-re-centrifugation, and concentrates the energy output by the dispersion equipment on the dispersion of small carbon nanotube bundles, ensuring The full dispersion of carbon nanotubes saves total energy consumption and sample preparation time, and is especially suitable for the preparation of carbon nanotubes containing more impurities and high-concentration carbon nanotube dispersions.

A core-shell structure poly-2,3-dimethylaniline / polyaniline conductive composite material and its preparation method, the structure of the composite material is poly-2,3-dimethylaniline as the core and polyaniline as the core of the shell It has a shell structure, and its preparation method is to firstly prepare poly-2,3-dimethylaniline by solution polymerization, and obtain its powder after suction filtration, washing, drying and grinding. Then use powdered poly-2,3-dimethylaniline as the core, and polymerize on its surface by solution polymerization or emulsion polymerization. After the product is filtered, washed, dried, and ground, the core-shell structure poly-2 , 3-Dimethylaniline / polyaniline conductive composites. The core-shell structure poly 2,3-dimethylaniline / polyaniline conductive composite material provided by the present invention significantly improves the conductivity of poly 2.3-dimethylaniline, which is beneficial to its application in sensors, conductive components, electrode active materials, etc. field applications.

The invention provides a method for constructing a gram-negative bacterium capable of expressing a gram-positive bacterium polysaccharide. The method comprises the following steps: transforming a low-copy expression plasmid of the gram-positive bacterium polysaccharide into an asd and rfbP gene-deletion gram-negative bacterium, screening by a balanced lethal system to obtain the gram-negative bacterium capable of producing the gram-positive bacterium polysaccharide. The invention also provides the gram-negative bacterium capable of producing the gram-positive bacterium polysaccharide prepared by the method. The invention also provides a mutant of Salmonella enterica serovar Typhimurium P0005, which is named as Salmonella enterica subsp. enterica serovar Typhimurium P0005, and has the CCTCC NO: M 2016344. The gram-negative bacterium can express the gram-positive bacterium polysaccharide, and the method provided by the invention does not contain any resistance marker and meets the biosafety requirements of subsequent vaccines and other application.

The invention discloses a culturing method for maintaining a self-renewal state of human embryonic stem cells. The method comprises passage and culturing of the human embryonic stem cells under the condition of using the culturing method and observation and detection of the self-renewal state of the human embryonic stem cells cultured under the condition of using the culturing method. According tothe method, the problems of easily differentiated state, complicated passage operation, high culturing condition cost and inconvenient subsequent research on mechanisms of the human embryonic stem cells under the current traditional culturing condition are solved by adding an inhibitor of a Wnt / beta-catenin signal channel; the method has the advantages of being stable in effect, economic and high-efficient, simple to operate, convenient to research and the like, clues for improving and establishing culturing conditions for multipotential stem cells of other kinds and species are provided, and the method has a positive promotion effect on smooth development of stem cell basis and application research in the future.

The invention discloses an in situ cultivation device, and a method of high through-put separating and screening microorganism. The in situ cultivation device comprises an in situ bacterium separatingdevice, the in situ bacterium separating device comprises a vacuum chassis, a clamping lower layer, a chip, a clamping pressing sheet and a clamping upper layer, the upper part of the vacuum chassisis provided with a vacuum groove, and lower part of the vacuum chassis is provided with a circular cylinder ring communicating with the vacuum groove, the clamping lower layer is connected with the top of the vacuum chassis in a fastening mode, and a base plate of the clamping lower layer is provided with through holes communicating with the vacuum groove, the chip is placed on the base plate of the clamping lower layer, the chip is evenly provided with a plurality of cultivation through holes, the clamping pressing sheet is arranged on the chip in a pressing mode, the clamping pressing sheetis provided with a plurality of sample-adding holes one-one corresponding to the cultivation through holes on the chip, the clamping upper layer is fixedly arranged above the clamping lower layer, andthe clamping upper layer is provided with a sample-adding window.

The invention provides a multi-province quinone polymer and a preparation method thereof, and the preparation method comprises the following steps: selecting modified montmorillonite as a template, and polymerizing polycyclic aromatic hydrocarbon and anhydride into the multi-province quinone polymer, the polymer has a nano lamellar structure.

The invention provides a method for preparing strontium niobate / strontium carbonate composite nanomaterials. The method includes: using SnNb 2 O 6 Nanomaterials are used as raw materials in a mixed alkali solution of potassium hydroxide and strontium hydroxide and CO 2 Hydrothermal reactions are carried out in the presence of The method provided by the invention has good repeatability, simple operation, is conducive to mass production, can greatly reduce costs, improve efficiency, and prepare SrNb 2 O 6 / SrCO 3 Composite nanomaterials have stable quality and have good application prospects in the field of photocatalytic water treatment, and can promote the development of metastable niobate crystallization methods and promote practical application research of niobate.

The invention relates to the technical field of terahertz imaging, in particular to a single-pixel imaging method and system based on terahertz free electron laser, and the main points of the technical scheme are that the method comprises the following steps: S1, providing a single-pixel imaging system based on terahertz free electron laser; and S2, on the single-pixel imaging system, obtaining a target object image according to a generalized orthogonal tracking algorithm. The method has the advantages of being good in reconstructed image quality, high in peak signal-to-noise ratio and high in structural similarity.

An ADSS (all dielectric self supporting optic fiber cable) typhoon resistance estimation method at a coastal region comprises following steps of S1, the nonoccurrence probability of an ADSS fault is calculated with a formula: P=A*sigma (X1, X2, X3...); A=A1*A2*...*An; in the formula, P is nonoccurrence probability of the ADSS fault and is in a hundred mark system; an ADSS nonoccurrence fault estimation coefficient is represented, a nonoccurrence fault estimation coefficient of estimation objects is represented by An, if an occurrence fault is non-maintainable or directly affects typhoon resistance, An is equal to 0, if the occurrence is maintainable or capable of recovering typhoon resistance, An is equal to 1; X1, X2, X3 ... respectively represent the weights of the occurrence fault probabilities of the estimation objects; the estimation objects comprise an ADSS self fault, an ADSS electrified corrosion state, an ADSS optical fiber attenuation state, an ADSS design standard application status quo, an ADSS sag state, applicable status quo of matched tools and engineering acceptance quality. The method promotes the application research of the ADSS in a typhoon environment, solves the problem existing in the application, and has an important significance in safe operation and development of power communication networks.

The invention provides a method for preparing composite alkali metal niobate powder, the method comprising: using Na 6 Nb 8 o 25 9H 2 O nanometer material is used as a raw material, and a hydrothermal reaction is carried out in a mixed alkali solution and in the presence of polyacrylamide, and the mixed alkali solution contains alkali metal ions in the composite alkali metal niobate. The method provided by the invention has good repeatability, simple operation, and low cost. The prepared composite alkali metal niobate powder, especially sodium potassium niobate and lithium sodium niobate, has good quality and is controllable, and can be used in nanometer devices. The field has good application prospects, and can promote the development of niobate crystallization methods and promote the practical application research of niobate.

The invention discloses a liquid crystal state composite fiber membrane as well as preparation and application thereof. The method comprises the following steps of mixing cholesteryl nonanoate and cholesteryl oleyl carbonate according to a formula ratio, heating until the temperatures of the two liquid crystal compounds reach the clearing point temperature or above, fully stirring until the two liquid crystal compounds are fully mixed, and cooling to obtain powder, so as to obtain CN-COC binary blended liquid crystal; and then adding the liquid crystal and a polyurethane elastomer into dichloromethane according to a formula ratio, stirring a mixture until solutes are completely dissolved, and obtaining the composite fiber membrane through an electrostatic spinning technology. The liquid crystal state composite membrane not only has liquid crystal state ordered fluidity and good biological performance, but also has the elastic characteristic of polyurethane, has good biological performance and mechanical performance, and can be used for constructing a biological membrane model imitating the extracellular matrix of the living body, and the influence of the liquid crystal performance of the living body on cell behaviors is researched on the basis, so that theoretical and technical supports are provided for ascertaining the interaction between the cells and the matrixes in the microenvironment of the living body.

An ADSS (all dielectric self supporting optic fiber cable) typhoon resistance estimation method at a coastal region comprises following steps of S1, the nonoccurrence probability of an ADSS fault is calculated with a formula: P=A*sigma (X1, X2, X3...); A=A1*A2*...*An; in the formula, P is nonoccurrence probability of the ADSS fault and is in a hundred mark system; an ADSS nonoccurrence fault estimation coefficient is represented, a nonoccurrence fault estimation coefficient of estimation objects is represented by An, if an occurrence fault is non-maintainable or directly affects typhoon resistance, An is equal to 0, if the occurrence is maintainable or capable of recovering typhoon resistance, An is equal to 1; X1, X2, X3 ... respectively represent the weights of the occurrence fault probabilities of the estimation objects; the estimation objects comprise an ADSS self fault, an ADSS electrified corrosion state, an ADSS optical fiber attenuation state, an ADSS design standard application status quo, an ADSS sag state, applicable status quo of matched tools and engineering acceptance quality. The method promotes the application research of the ADSS in a typhoon environment, solves the problem existing in the application, and has an important significance in safe operation and development of power communication networks.

The invention discloses an ultra-thin silicon nanosheet photocatalyst and its preparation method and application. It uses crystalline silicon powder as a precursor, disperses it into a solvent after being dipped in an ultra-low temperature liquid gas, and then assisted the liquid phase by ultrasonic waves. The ultra-thin silicon nano-sheet suspension is obtained by peeling off, and after the suspension is centrifuged and freeze-dried, an ultra-thin silicon nano-sheet photocatalyst with a thickness of 0.3-2.1 nm and a size of 0.1-6.5 μm is obtained. When the silicon nanosheets obtained in the present invention are used for visible light photocatalytic hydrogen production, the hydrogen production efficiency can reach 5.102 mmol h ‑1 g ‑1 , the photocatalytic hydrogen production efficiency is about 93 times higher than that of the original crystalline silicon powder. The method for preparing silicon nanosheets disclosed by the invention is simple to operate, low in cost, and can be prepared in large quantities, and the obtained silicon nanosheets have excellent visible-light photocatalytic hydrogen production performance, providing a new method for building stable and efficient photolysis of water for hydrogen production materials. train of thought.

The invention discloses a control system and method of a bipolar high voltage pulse power supply. A switching sequence in a solid switch group module through control modules in a high voltage positivepulse generating circuit and a high voltage negative pulse generating circuit to obtain the bipolar high voltage pulse power supply in which a positive / negative pulse rising time, a positive / negative pulse falling time, a time interval between positive and negative pulses within one period, a positive / negative pulse voltage, a positive / negative pulse width, pulse frequency and a pulse number can be flexibly adjusted. The parameter-adjustable bipolar pulse realized by the control system and method disclosed by the invention has very important significance for studying the mechanism and the application of nanosecond pulse discharge.