94results about How to "Low miss rate" patented technology

The invention relates to the technical field of gene knockout, and particularly discloses a method for breeding a tcf25 gene deletion type zebra fish through gene knockout. The method comprises the steps of through a CRISPR/Cas9 gene editing technology, designing an appropriate targeting gene locus on a tcf25 gene of the zebra fish, synthesizing in vitro to obtain specific sgRNA and Cas9-mRNA, microinjecting into a cell of the zebra fish, and after culturing an embryo for 60h, selecting the embryo for carrying out genotyping, so that the effectiveness of the selected locus is verified. The method provided by the invention is lower in off-target rate, removes the tcf25 gene through interference, is beneficial to further revealing the whole process of cardiac morphogenesis and a molecular mechanism regulating the process through researching functions through a genetics means, and is of great importance on understanding a cardiac disease pathology and researching and developing a new therapeutic schedule medically.

The invention discloses a CRISPR-Cas9 system used for assembling DNA and a DNA assembly method. The CRISPR-Cas9 system includes the following parts: a plasmid used for expressing Cas9 gene, a first guide RNA, and/or a plasmid used for expressing the first guide RNA, wherein the first guide RNA has a CRISPR site. The CRISPR site is combined with a first reporter gene carried by a semisynthetic chromosome used for assembling according to base complementation pairing rule. The CRISPR-Cas9 system has advantages of high replacement success rate of homologous recombination, less species of guide RNA which needs to design and use, less harmful effect subjected by the genomic sequence, and low off-target rate.

The invention relates to a generation method of a high-satisfaction video summary. Based on the characteristics of a video data structure, a shot cluster-based video summary system is designed and realized in the invention. The video summary system has the following main functions: coding, decoding and playing of various video files, shot boundary detection, key frame extraction, shot cluster, generation of both static video summary and user input time dynamic summary, and the like. The generation method is suitable for various application occasions, such as multimedia file management, video retrieval, and film and television database construction.

The invention discloses a fusion protein in a Cpf1 and p300 core structural domain, a corresponding DNA target activation system and application. CRISPR/Cpf1 with enzyme cutting activity is mutated to be free of enzyme cutting activity and of target gene identification characteristic, is fused with p300 protein transcriptional activation, reaches the purpose of target gene activation inside mammalian cells, and has the characteristics of being simple, efficient and high in specificity. The fusion protein has the advantages of low off-target rate, cost saving and the like. The system can be mutually complementary with an existing target gene activation method based on CRISPR/Cas9, the gene editing range of the CRISPR/Cas9 system is expanded, and the system has a good application prospect.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 recombinant lentiviral vector containing gRNA sequence specifically targeting CCR5 and application thereof. A lentivirus of the CRISPR/Cas9 recombinant lentiviral vector containing gRNA sequence specifically targeting CCR5 gene Delta 32 region is constructed that the lentivirus can introduce cells into a CRISPR/Cas9 system specific to CCR5, double-chain breakage occurs to a specific site of CCR5 gene, a random mutation is introduced to a breakage site after repairing by means of nonhomogeneous recombinant terminal binding, and the mutation rate reaches 90% and above. As gRNA is a nonhomogeneous region of CCR5 and CCR2, detection shows that the missing efficiency of the two gRNAs is lower than 0.2%. Cells modified via the recombinant lentivirus have significantly decreased efficiency of HIV (human immunodeficiency virus) infection. The system is quick to construct, simple and low in price, and is applicable to gene therapy of acquired immune deficiency syndrome.

The invention belongs to the field of aquaculture equipment engineering, and particularly relates to a double-ship continuous operation type deep sea shellfish fishing and catching system and a fishing and catching thereof. The method comprises the steps that an ecotype bottom trawl fishes and catches marine demersal shellfish into a net on the condition that the bottom environment is not influenced under the traction of a dragging ship, the collected shellfish collect in a fish bag of the ecotype bottom trawl under the action of water current and netting gears, when an online video monitoring device observes that the fish catch of the shellfish in the fish bag reaches a fishing and catching requirement, the shellfish are indrawn on a fishing and catching ship by a conveying pipeline fixed on the fish bag under the action of a self-priming pump, and a continuous fishing and catching process is completed. According to the double-ship continuous operation type deep sea shellfish fishing and catching system and the fishing and catching thereof, the shellfish catches in the ecotype bottom trawl can be conveyed to the fishing and catching ship in time, there is no need to repeat net hauling and net shooting, accordingly the operation time is saved, the leakage capture rate is reduced, the bottom environment and resources are protected, and the fishing and catching system and the fishing and catching thereof are especially suitable for the continuous fishing and catching operation of marine pasture area large-area bottom sowing shellfish.

The present invention discloses a capture method for sine binary offset carrier modulation signals. According to the capture method and apparatus, sine binary offset carrier signals are adopted as special time-division multiplexed modulation signals; under a local correlation condition, a plurality of paths of pseudo-random code signals are adopted; time division correlation-removing operation is performed on carrier-removed intermediate-frequency signals and the plurality of paths of pseudo-random code signals; the independent coherent integration values of each path are calculated; and the correlation results of all branches are combined, so that a final correlation result is obtained. With the time-division correlation method adopted, the influence of sub-peaks and zero points in an autocorrelation function can be effectively removed, and the probability of missed capture can be reduced. The capture method is similar to a traditional BPSK (binary phase shift keying) signal capture method. Compared with existing methods, the method of the invention is easier to implement and can assist in reducing system complexity. The present invention also provides an apparatus device for implementing the above method. Since cross-correlation or sideband energy loss are avoided, so that signal acquisition sensitivity can be improved. The capture method and the apparatus of the present invention therefore have high commercial value.

The present invention discloses a method for splitting Cas9 and an application. The present invention provides a method for splitting Cas9 protein at a plurality of sites of the Cas9 protein into different segments of amino acid sequences. The Cas9 protein is spCas9(D10A) protein. The split Cas9 protein can be transported into target cells/vectors more conveniently, and can be recombined by meansof intein. Accordingly, the present invention provides proteome and fusion proteome obtained by the method and nucleic acid constructs and vectors for expressing the fusion proteome, and engineering cells. The fusion proteome and the vectors can be used for editing cytogenes, or in targeted positioning or gene expression transcription activation or gene expression transcription inhibition, and used in preparation of pharmaceutical preparations for gene editing.

The invention relates to the technical field of gene knockout, in particular, the invention discloses a gene knockout breeding method for tnni3k gene deletion zebrafish, which adopts CRISPR/Cas9 geneediting technology to design a suitable target site on the tnni3k gene of zebrafish, and synthesizes specific gRNA and Cas9-protein was co-injected into zebrafish cells. After 48 hours of embryo culture, the embryos were selected for genotypic analysis, which confirmed the validity of the selected sites. The invention can more efficiently and more accurately silence a specific gene in the genome of an organism, and has the advantages of simple production, low cost, and can simultaneously cut a plurality of loci on a target gene and silence any number of single genes. At that same time, zebrafish embryo interfered with the tnni3k gene appear obvious developmental abnormalities.

The embodiment of the invention discloses a signal capture method and device. The method and the device are applied to a time division and code division orthogonal frequency division multiplexing TC-OFDM system. The method comprises the steps of carrying out incoherent loss compensation on an incoherent integral result Z (n), judging whether the incoherent integral result after the incoherent loss compensation satisfies a preset threshold judging condition or not; and capturing signals if the preset threshold judging condition is satisfied. According to the method and the device, through combination of coherent/incoherent integrals and loss compensation of the incoherent integral result, the signals are captured; a relative capture peak can be effectively improved; the capture missing probability of weak signals can be reduced; therefore, the capture capability of the location receiver of the TC-OFDM system to the weak signals can be improved; and the weak signals can be captured effectively.

The invention discloses a polyoligonucleic acid molecule and its application in multi-target interference. Through combination of a CON technology and a nanotechnology, the nucleic acid structure withan accurate and self-assembling capacity is constructed so that multi-target interference is realized. The polyoligonucleic acid molecule can be used for inhibiting expression of multiple genes in asignaling pathway of disease occurrence or development, or simultaneously inhibiting the expression of multiple disease target genes, and has broad application prospects in many fields such as biologyand chemistry. The experiment result shows that the polyoligonucleic acid molecule has the advantages of 1) high RNAi efficiency, 2) good chemical stability, strong anti-DNA enzyme degradation ability and long half-life, 3) low off-target rate, and 4) formation of nanoparticles with plasticity and strong cell introduction ability.

Provided are a Cas effector protein, a fusion protein comprising the protein, and a nucleic acid molecule encoding the same. Also provided are complexes and compositions for nucleic acid editing, such as gene or genome editing complexes and compositions comprising Cas effector proteins or fusion proteins, or nucleic acid molecules encoding them. Also provided are methods for nucleic acid editing, such as methods for gene or genome editing, which use Cas effector proteins or fusion proteins.

The invention relates to the technical field of gene knock-out, in particular to a method for selecting and breeding an ALPK2 gene-deleted zebrafish through gene knock-out. The method comprises the steps of designing multiple proper targeting sites on an ALPK2 gene of the zebrafish by means of a CRISPR/Cas9 gene editing technology, and synthesizing specific gRNA and Cas9-protein in vitro; conducting microscopic injection of gRNA and Cas9-protein into a cell of the zebrafish, and after 48 hours of embryo culture, selecting embryos to carry out genotype analysis, so that the effectiveness of theselected site is confirmed. The method can more efficiently and accurately silence specific genes in the genome of an organism, manufacturing is easy, and the cost is low. Moreover, the sites on thetarget gene can be simultaneously sheared at the same time to silence any number of single genes. Additionally, development deformity of the zebrafish embryos with the ALPK2 gene is interfered.

The invention relates to the technical field of gene editing, and particularly discloses a SlugCas9HF-protein, a recombinant vector containing the protein, a CRISPR/Cas9-HF gene editing system, application and the like. The SlugCas9-HF protein has the amino acid sequence shown in SEQ ID NO:1 or is at least 80% identical to SEQ ID NO:1 and retains at least one or more of R247A, N415A, T421A and R656A mutations, and the recombinant vector containing the protein is obtained by connecting a gene sequence encoding amino acid of the SlugCas9-HF protein to a base vector. The CRISPR/Cas9-HF gene editing system is composed of SlugCas9-HF protein and sgRNA, which can edit target genes with high specificity and high editing efficiency, and the off-target rate is within 2.87%.

The invention provides an assembling quality online detection method, device and system. The method is characterized by collecting vibration signals during an assembling period, dividing the vibrationsignals in the assembling period into several segments and taking as the vibration signal of each assembling process in the assembling period; calculating the autocorrelation function of the vibration signal of each assembling process through using an autocorrelation or cross-correlation method, and constructing the error tolerance range of each assembling process; collecting the vibration signalof each assembling process after set assembling time, calculating the autocorrelation or cross-correlation function of the vibration signal, and recording a time interval corresponding to the peak value and the peak point of the autocorrelation or cross-correlation function; determining whether the time interval corresponding to the peak value and the peak point of the autocorrelation or cross-correlation function of each assembling process satisfies the corresponding error tolerance range; and if the time interval satisfies the corresponding error tolerance range, determining that assemblingoperation quality accords with a requirement, otherwise, determining that the assembling operation quality does not accord with the requirement. By using a correlation technology, the on-line detection of assembling quality is performed and component error and leak rates can be greatly reduced.

The invention discloses a method for carrying out gene mutation on rhodobacter sphaeroides. The invention provides a method for carrying out gene point mutation on rhodobacter sphaeroides to obtain amutant strain. The method comprises the following steps: on basis of a gene editing technology, editing a genome of rhodobacter sphaeroides by using a fusion protein, and obtaining the mutant strain through screening, wherein the fusion protein contains a protein with a DNA targeting function and an enzyme with a mononucleotide directional mutation function. The method disclosed by the invention has the advantages that the whole flow is simple to operate and steps are few; regulation aiming at a specific metabolic pathway is more accurate and strict and the miss rate is low; and the method hasuniversal applicability and is a novel microbial gene transformation technology with an important application value in the aspects of industrial production, environmental protection and the like.

The invention discloses an automatic elimination thickness meter and belongs to the field of measurement equipment. The automatic elimination thickness meter comprises a supporting column, a thickness measuring box, a thickness measuring mechanism and an elimination mechanism, wherein the thickness measuring box is arranged on the supporting column; a touch screen is arranged on the thickness measuring box; a sounding outlet is formed in the top of the thickness measuring box; the elimination mechanism is used for eliminating defective products; the thickness measuring mechanism is electrically connected with the elimination mechanism; when the thickness measuring mechanism detects the defective products, a cylinder drives a limiting plate to move downwards, and the defective products on a conveying belt can slide out of a production line along the inclined limiting plate and are separately recovered. The degree of automation is high, manpower is saved, and the omission rate of the defective products is low.

The invention relates to the technical field of gene knockout, and particularly discloses a method for selectively breeding bai2 gene deletion zebra fish through gene knockout. Through a CRISPR/Cas9 gene editing technique, suitable targeting loci are designed on a bai2 gene of the zebra fish, specific gRNA synthesized in vitro enters a cell of the zebra fish in a micro-coinjection mode, after embryo culture is conducted for 50 h, genotype analysis is conducted by selecting embryos, and thus the effectiveness of the selected loci is verified. According to the method, specific genes can be silenced more efficiently and more precisely in a living body genome, manufacturing is easy, the cost is low, the multiple loci on the target gene can be sheared simultaneously, and the any number of single genes are silenced; and expression of the bai2 gene is interfered, the function of the bai2 gene is studied in a genetics mode, the whole process of cardiac morphology generation and the molecular mechanism of adjusting and controlling the processes are further disclosed advantageously, and the method is of great significance to understanding of pathology of cardiac diseases in medicine and development of new treatment schemes.