36 results about "Rhodobacter" patented technology

The invention discloses a high-yield lycopene recombinant rhodobacter sphaeroides strain as well as a construction method and application thereof, and belongs to the technical field of biosynthesis and biological energy sources, the construction method comprises the following steps: knocking out a crtC gene of rhodobacter sphaeroides by using a homologous recombination method to obtain a recombinant strain RS01; the method comprises the following steps: on the basis of a strain RS01, replacing a crtI3 gene on a rhodobacter sphaeroides genome with a crtI4 gene (from rhodospirillum rubrum) by using a homologous recombination method to obtain a recombinant strain RS02; the method comprises the following steps: connecting crtB and crtE genes to a pYYDT vector to construct a plasmid pYYDT-crtB-crtE, transferring the plasmid pYYDT-crtB-crtE into escherichia coli S17, and then introducing the plasmid pYYDT-crtB-crtE into a recombinant rhodobacter sphaeroides RS02 in a conjugational transfer manner to obtain a recombinant strain RS03. The recombinant rhodobacter sphaeroides constructed by the invention can be used for efficiently producing lycopene.

The invention belongs to the technical field of food and biological fermentation, and particularly relates to a rhodobryum tofu polysaccharide extract as well as a preparation method and application thereof. The preparation method of the rhodobryum tofu polysaccharide extract comprises the following steps: extracting rhodobryum tofu with hot water, filtering, taking filtrate, and concentrating to obtain a rhodobryum tofu aqueous extract; and precipitating the obtained aqueous extract with ethanol, centrifuging, taking the precipitate, dissolving the precipitate in ionized water, then removing protein by adopting a Sevag method to obtain a crude polysaccharide solution, filling the crude polysaccharide solution into a dialysis bag, dialyzing to remove small molecular substances, and freeze-drying to obtain the rhodotorula tofu polysaccharide extract. The polysaccharide functional components are extracted from the red fungus tofu as a raw material, and the polysaccharide content and component differences of the red fungus tofu obtained by different fermentation strains are verified. The application effect of the rhodobryum tofu polysaccharide extract in reducing the content of effective heavy metal Pb in food is verified, and the rhodobryum tofu polysaccharide extract has a good prospect in reducing the bioavailability of the heavy metal in the food, reducing the toxicity of the heavy metal and improving the food safety.

The invention provides a metabolic engineering transformation method of high-yield coenzyme Q10 of rhodobacter sphaeroides and application of transformed engineering bacteria. The method comprises the following steps: knocking out geranyl and geranyl pyrophosphate synthase gene crtE participating in a carotenoid synthesis route; performing overexpression on a farnesyl pyrophosphate synthase gene ispA; a histidine kinase coding gene prrB is mutated, and the mutant is introduced. According to the invention, a combined regulation strategy suitable for targeted regulation is found, the metabolic flux synthesized by the coenzyme Q10 is accurately optimized, and the capability of producing the coenzyme Q10 by the rhodobacter sphaeroides is greatly improved.

The invention discloses construction of a shewanella hydrogengens-CdS nanoparticle hybrid system based on a hydArsp gene and an application of the shewanella hydrogengens-CdS nanoparticle hybrid system. The method comprises the following steps: connecting a hydArsp gene derived from Rhodobacter sphaeroids coded hydrogenase to a vector pYYDT, carrying out ribosome optimization transformation to obtain a plasmid pYYDT-hydArsp, introducing the recombinant plasmid pYYDT-hydArsp into wild shewanella oneidensis MR-1 to obtain a recombinant shewanella engineering bacterium SERS with high hydrogen production efficiency, anchoring CdS nanoparticles on periplasm and an extracellular membrane of a strain in a self-assembly manner, and carrying out high-yield hydrogen production on the recombinant shewanella engineering bacterium SERS. The CdS / SERS high-hydrogen-yield active hybrid system is obtained. The CdS / SERS hybrid system constructed by the invention endows shewanella with light capturing ability, CdS nanoparticles provide photoelectrons for engineering bacteria to promote intracellular reducing power and ATP regeneration, and the hybrid system constructed by the invention can enhance the proton reduction rate of the engineering bacteria and improve the hydrogen production efficiency.

The invention relates to the technical field of sewage treatment, discloses a sewage treatment dynamic load optimization method and system based on rhodobacter online monitoring, and aims to solve the problems of unstable process, effluent fluctuation, increase of energy consumption and material consumption and the like caused by lack of real-time accurate biological phase indication when the existing sewage treatment is used for coping with dynamic change of inflow load. According to the method and the system, biomass and physical and chemical parameters are obtained through online monitoring of rhodobacter and sewage parameters; after data preprocessing, dynamic load analysis is carried out to predict inflow water / biological load and a rhodobacter growth trend; the optimization decision module generates and controls the execution module to implement key operation parameter adjustment; and the man-machine interaction alarm module carries out visual management. According to the method, real-time quantitative evaluation of the biological phase is realized, the dynamic load adaptability, the operation efficiency and the effluent stability of the system are remarkably improved, and the energy consumption and the material consumption are reduced.

The invention discloses an intratumor microbial marker group for gastrointestinal tumor prognosis, a prognosis model and application. The biomarker group comprises the following components: Granulicella, Thermodesuftator, Gaetbus, Formosa, Kaistobacter, Candidate Saccharimonas, Dactyloccopsis, Schwartzia, Candidate Kuenia, Sphingosinusus, Microvirgula, Simple, Doria, Halothiobacillus, Rhodobacter, and Oceaniterimus, and is characterized in that the biomarker group comprises the following components: the Granulicella, the Thermodesuftator, the Gaetbus, the Formosa, the Kaistobacter, the Candidate Saccharimonas, the Dactyloccopsis, the Schwartzia, the Candidate Saccharimonas, the The model constructed by the method can accurately predict prognosis, drug response and immunotherapy.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The invention discloses an artificially synthesized corrinic acid operon, and the sequence of the artificially synthesized operon is obtained by integrating corrinic acid synthesis related genes and connecting and constructing the corrinic acid synthesis related genes in a reordering and end-to-end manner. According to the operon, a recombinant strain is synthesized on the basis of various chassis strains such as escherichia coli and rhodobacter sphaeroides, the corrinic acid and the derivatives thereof are synthesized through fermentation, the corrinic acid and the derivatives thereof can also be synthesized through a cell-free catalytic system, the problem that overlapping genes of a natural operon are difficult to modify is solved, and the synthesis efficiency and controllability of the corrinic acid are improved.

The invention belongs to the technical field of microbial fermentation and plant disease prevention and treatment, and particularly relates to application of a compound microbial agent to prevention and treatment of citrus huanglongbing. According to the present invention, the photosynthetic bacteria such as Rhodobacter sphaeroides A13 and the Bacillus velezensis XZT106 are mixed to obtain the microbial agent, such that the significant effect of the microbial agent on the prevention and control of the citrus huanglongbing is found, the leaf yellowing rate is reduced to 6.67%, and the red nose occurrence rate is reduced to 11.11%.

The invention relates to the technical field of microorganisms, in particular to a compound microbial agent as well as a preparation method and application thereof. The invention provides a compound microbial agent. The effective components of the compound microbial agent are fermentation liquor of rhodobacter sphaeroides GTW-A1 and fermentation liquor of bacillus licheniformis GTW-B2, the preservation number of the rhodobacter sphaeroides GTW-A1 is CGMCC (China General Microbiological Culture Collection Center) No.33609, and the preservation number of the bacillus licheniformis GTW-B2 is CGMCC No.33610. The invention further discloses a preparation method of the The compound microbial agent can effectively increase the carbon reserve of soil, passivates the activity of heavy metals through the synergistic effect of bacterial strain metabolites, improves the soil structure and promotes ecological restoration of microorganisms.

The invention belongs to the technical field of microbial fermentation, and discloses a method for screening coenzyme Q10 producing bacteria by using rhamnolipid, which comprises the following steps: coating a rhamnolipid-containing solid culture medium with rhodobacter sphaeroides, culturing, selecting single colonies, inoculating the single colonies into a rhamnolipid-containing fermentation culture solution as a seed solution, and culturing to obtain the coenzyme Q10 producing bacteria by using the rhamnolipid-containing fermentation culture solution. And then carrying out fermentation culture in a fermentation culture solution containing the rhamnolipid for 72 hours, collecting thalli, and measuring the content of the coenzyme Q10, so as to obtain the coenzyme Q10 high-yield strain. According to the method, the coenzyme Q10 production fungus rhodobacter sphaeroides is directionally screened, so that the strain of which the yield of coenzyme Q10 is remarkably increased can be efficiently obtained, the yield can be stably inherited, and the industrial requirements are met.

The present invention relates to the field of biotechnology, and particularly to a Rhodobacter sphaeroides and its application in de novo synthesis of oxidized coenzyme Q10. The present invention provides Rhodobacter sphaeroides ( Rhodobacter sphaeroides ) INGIA-NM002, with the preservation number of CGMCC No. 32823. Using this strain to ferment and produce oxidized coenzyme Q10 with substrates (glucose or glycerol) can improve the yield and efficiency of industrial production to meet market demands.

The invention provides a high-salt-resistant composite biological bacterial agent as well as a preparation method and application thereof. The high-salt-resistant composite biological bacterial agent is prepared from bacillus subtilis, bacillus licheniformis, phosphate solubilizing bacteria, photosynthetic bacteria, saline-alkaline rhodobacter and lactobacillus acidophilus. According to the method, the salt content in the culture medium is gradually increased through three times of domestication culture, so that the strain gradually adapts to a high-salt environment. The high-salt-resistant composite biological agent can be used for treating mine water and strong brine. According to the invention, a plurality of salt-tolerant strains are selected for a high-salt environment, during water treatment, the strains can form a complex ecological network by metabolizing pollutants in mine water or strong brine, and through synergistic effect, smooth and efficient operation of material circulation and energy flow in the mine water or strong brine is promoted, so that the water treatment effect is improved. A good biodegradation effect can be achieved on mine water and strong brine.

The application provides a preparation method of a Rhodobacter sphaeroides CsCobT and CsCobN protein complex in vitro, and steps are as follows: constructing an expression vector pET-28a(+)‑Cs CobN , pET-28a(+)‑SUMO‑Cs CobT ; expressing and purifying His-CsCobN protein; preparing a non-tagged CsCobT protein; mixing the purified His-CsCobN protein and the non-tagged CsCobT protein to form a protein complex; separating the complex through affinity chromatography; and refining the complex through gel filtration chromatography. The application adopts a strategy of double vectors, step-by-step purification, in-vitro assembly and multi-dimensional verification, can obtain a CsCobT and CsCobN protein complex with high purity and uniform conformation, and the experimental process can be accurately controlled, the result is specific, and provides high-quality protein materials for subsequent molecular mechanism research and application development of cobalt-chelating enzyme.

The application provides a promoter library, a construction method of the promoter library and application of the promoter library. The promoter library of the application comprises promoters derived from Rhodobacter sphaeroides or modified variants thereof. The application discloses nucleic acid sequences of each promoter in the promoter library, vectors containing the nucleic acid sequences of the promoters and host cells. The application also discloses a screening method of the promoter library, and a promoter library with a gradient distribution of strength is obtained, so that high expression or low expression of a target gene can be realized and / or fine adjustment of expression thereof can be realized.

A ginseng endophyte with high PQQ production, characterized in that: the taxonomic name of the ginseng endophyte is Rhodobacter methylophilus Methylorubrum sp.) W7N-70, which is deposited in the China Center for Type Culture Collection (CCTCC), with the deposit address being No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the deposit number being CCTCC M 20241885, and the deposit date being September 2, 2024. The ginseng endophyte W7N-70 obtained in the present invention has the ability to produce PQQ, and the detected PQQ synthesis amount in its supernatant reaches 75.81 mg / L, which can be effectively applied to the fermentation engineering for synthesizing PQQ. In addition, this strain can produce antibacterial metabolites and can effectively inhibit the growth of various pathogenic bacteria. This strain has excellent effects when applied to promoting plant growth and antibacterial, as well as synthesizing PQQ.

The invention discloses recombinant Saccharomyces cerevisiae for producing decaisopentenol as well as a construction method and application of the recombinant Saccharomyces cerevisiae, and belongs to the technical field of microorganisms. The preparation method comprises the following steps: expressing a decaisopentenyl pyrophosphate synthase gene from Rhodobacter sphaeroides in saccharomyces cerevisiae, and carrying out fermentation on the decaisopentenyl pyrophosphate synthase gene to obtain the decaisopentenyl pyrophosphate synthase. Overexpressing the endogenous phosphatidic acid phospholipase gene of the saccharomyces cerevisiae; the recombinant saccharomyces cerevisiae is obtained in a manner of reducing the transcriptional level of a squalene synthesis gene ERG9 and strengthening an MVA endogenous way. According to the method, the production of the decyl isopentenol is realized in the saccharomyces cerevisiae for the first time, the shake flask yield reaches 112mg / L, then the recombinant saccharomyces cerevisiae is fermented to extract the decyl isopentenol, and then the coenzyme Q10 is synthesized by a chemical method, compared with the traditional method for producing the coenzyme Q10 by fermenting the rhodobacter sphaeroides, the method has the advantages of high fermentation stability, simple culture medium and the like, and the method is suitable for industrial production. The industrial application potential is realized.

This invention provides a 5-aminolevulinic acid producing strain, its construction method, and its application. Based on the existing strain JAL9, it involves knocking out… pflB、ldhA、poxB、adhE、ackA、dppA Genes, upregulated eamA、grxA、sodB The transcriptional level of the katE gene was downregulated. pfkB Gene transcription level, heterologous expression hemA ST Genes and xfp The gene, along with a high-copy plasmid PETC4, expresses genes from... Rhodobacter capsulatus of hemA RC Genes, ultimately through hemB Introducing an ASV degradation tag at the gene terminus enhances the degradation of HemB protein, effectively improving the synthesis efficiency of 5-aminolevulinic acid. This method has good industrial application value when applied to the fermentation production of 5-aminolevulinic acid.

The invention relates to the technical field of biology, in particular to a method for de novo synthesis of reduced coenzyme Q10. The method provided by the invention is based on coenzyme Q10 reductases from five sources and takes oxidized coenzyme Q10 as a substrate for preparation, the oxidized coenzyme Q10 can be synthesized from Rhodobacter sphaeroides INGIA-NM002 through special condition parameters, and the whole process flow has the advantages of high yield, high purity, low cost, high safety, suitability for industrial production and the like.

The invention discloses a recombinant rhodobacter sphaeroides strain for synthesizing lycopene by photocatalytic immobilization of CO2 and a construction method and application thereof, and the construction method comprises the following steps: knocking out an endogenous crtC gene of rhodobacter sphaeroides by using a homologous recombination method, and replacing a crtI3 gene of rhodobacter sphaeroides by using a crtI4 gene from rhodobacter rubrum to obtain a recombinant strain RS-1; a crtB gene and a crtE gene are connected to a pYYDT vector, transferred into escherichia coli S17 and then introduced into a recombinant rhodobacter sphaeroides RS-1 through conjugational transfer, and a recombinant strain RS-2 is obtained. The recombinant rhodobacter sphaeroides constructed by the invention can be used for de novo synthesis of lycopene by using CO2 as a carbon source through photocatalysis. The method is simple to operate, and meanwhile, high-yield lycopene can be obtained.

The invention discloses a nitrogen limitation-based clostridium pasteurianum-rhodobacter sphaeroides coupling fermentation hydrogen production method and application thereof. The method comprises the following steps: performing dark fermentation on activated clostridium pasteurianum in a nitrogen-free culture medium in an argon environment to produce hydrogen; and adjusting the pH value of the obtained culture solution to 7.0-8.0, selectively sterilizing, inoculating activated rhodobacter sphaeroides, and carrying out light fermentation in an argon environment to produce hydrogen. The clostridium pasteurianum fermentation liquor can be directly subjected to rhodobacter sphaeroides photofermentation hydrogen production culture only by simply adjusting the pH value without operations such as centrifugal removal of thalli. According to the method, excessive proliferation of thalli is inhibited through nitrogen limitation, the flow direction of a carbon source is saved, high expression of nitrogenase is induced, and a second efficient hydrogen production way except hydrogenase is opened up, so that the hydrogen yield is further increased while the cost of a nitrogen source is reduced. The hydrogen production yield reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The application discloses a method for producing hydrogen through Clostridium pasteurianum-Rhodobacter sphaeroides coupling fermentation based on nitrogen limitation and application thereof. In the method, activated Clostridium pasteurianum is subjected to dark fermentation for producing hydrogen in a nitrogen-free culture medium and in an argon environment; then, the pH value of the obtained culture solution is adjusted to 7.0-8.0, the activated Rhodobacter sphaeroides is inoculated after selective sterilization, and the light fermentation for producing hydrogen is carried out in the argon environment. In the application, the fermentation solution of Clostridium pasteurianum does not need to be subjected to centrifugal removal of bacterial bodies and the like, and only needs to be simply adjusted in pH value, so that the light fermentation for producing hydrogen of Rhodobacter sphaeroides can be directly carried out. In the application, the nitrogen limitation is used to inhibit excessive proliferation of bacterial bodies, save carbon source flow, induce high expression of nitrogen fixation enzyme, and open a second high-efficiency hydrogen production path in addition to hydrogenase, so that the hydrogen production yield is further improved while the cost of nitrogen source is reduced. The hydrogen production yield of the application reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The application discloses a mutant strain with high coenzyme Q10 yield, and has a preservation number of CGMCC No.22200.The mutant strain is obtained by enrichment, separation, mutagenesis and screening of Rhodobacter sphaeroides with high coenzyme Q10 content.The application also discloses application of the mutant strain in production of coenzyme Q10 and in medicines, health products, cosmetics and food.

The present invention belongs to the technical field of ionic organic conjugated microporous polymer-Rhodobacter sphaeroides biohybrids, and particularly relates to an ionic organic conjugated microporous polymer-Rhodobacter sphaeroides biohybrid, its preparation method, and application. An ionic organic conjugated microporous polymer and Rhodobacter sphaeroides biohybrid are constructed under anaerobic and carbon-free conditions. The constructed organic conjugated microporous polymer-Rhodobacter sphaeroides biohybrid fixes CO2 and produces carotenoids under anaerobic conditions. The ionic organic conjugated microporous polymer-Rhodobacter sphaeroides biohybrid constructed by the present invention has a high CO2 fixation capacity under anaerobic conditions, uses CO2 as the sole carbon source for metabolism, and can efficiently synthesize carotenoids.

The present invention relates to the field of biotechnology, and particularly to a method for de novo synthesis of reduced coenzyme Q10. The method provided by the present invention is based on the preparation of coenzyme Q10 reductase from five sources using oxidized coenzyme Q10 as a substrate. The oxidized coenzyme Q10 can be de novo synthesized by Rhodobacter sphaeroides INGIA-NM002 under special condition parameters. The whole technological process has the advantages of high yield, high purity, low cost, high safety, and being more suitable for industrial production, etc.

The invention provides a citrus planting method, which is characterized in that a diluent of rhodobacter sphaeroides concentrated metabolite is applied in a root irrigation mode in a fruit expansion period of a citrus fruit tree. The application finds that the rhodobacter sphaeroides concentrated metabolite can help to delay the ripening of citrus fruits and improve the fruit quality, and when the rhodobacter sphaeroides concentrated metabolite is used for irrigating the red beauty in a specific root irrigation mode, the content of soluble solids in the red beauty fruits can be increased, and the fruits can be promoted to mature later to accumulate more nutritional ingredients; meanwhile, it is accidentally found that the copper content in the soil for planting the Hongmeiren is remarkably reduced, and the zinc content is remarkably increased, it is proved that according to the citrus planting method, citrus fruits higher in nutrition and flavor value can be obtained, and meanwhile copper toxicity caused by the acid soil environment in plants can be reduced.

The application discloses a method for improving acid aluminum tolerance of sugarcane by high-efficiency combined nitrogen fixation, and relates to the technical field of sugarcane cultivation. The method comprises the following steps: controlling the nitrogen fertilizer amount, that is, only the bottom fertilizer applied in the whole growth period of the sugarcane contains the nitrogen fertilizer, and the fertilizer applied in other periods does not contain the nitrogen fertilizer; constructing a high-efficiency combined nitrogen fixation system, that is, applying the bacteria agent of Beijerinckia and the enhancer when the sugarcane is newly planted; applying the bacteria agent of Rhodobacter sphaeroides and the enhancer in the sugarcane seedling stage; applying the bacteria agent of Lipomyces and the enhancer in the sugarcane tillering stage; and the enhancer comprises indole acetic acid, eugenol and vitamin E. The method can make the sugarcane grow normally in the high-acid aluminum soil by controlling the nitrogen fertilizer amount, selecting suitable nitrogen fixation bacteria in different growth periods of the sugarcane and adopting a series of means such as the auxiliary use of the enhancer to construct the high-efficiency combined nitrogen fixation system.

The invention relates to an antibiotic-label-free inducible salt-tolerant rhodobacter sphaeroides engineering bacterium and application thereof, and belongs to the technical field of microorganisms. Wild rhodobacter sphaeroides is a dominant strain for biological sewage treatment, but natural strains grow very slowly when the salt concentration exceeds 3%, and the pollutant degradation capacity is obviously inhibited. Compared with wild strains and constitutive overexpression engineering bacteria, the inducible salt-tolerant rhodobacter sphaeroides engineering bacteria constructed by the invention have the advantages that the treatment capacity on ammonia nitrogen, phosphate and acetate in 3-5% high-salt sewage after lactose induction is obviously improved; technical support is provided for application of the rhodobacter sphaeroides in the fields of industrial wastewater purification in coastal areas, ecological restoration of saline-alkali soil, seawater recycling and the like, and the competitiveness of the rhodobacter sphaeroides in low-cost seawater culture, high-salinity wastewater treatment and special environment biological manufacturing is remarkably improved.

This invention relates to the field of water treatment and discloses a strain of *Methylorubrum aminovorans* and its application in treating high-concentration formaldehyde wastewater. This *Methylorubrum aminovorans* strain is named 3-5-6, and its microbiological classification is *Methylorubrum aminovorans*. It was deposited at the China General Microbiological Culture Collection Center (CGMCC) on November 24, 2025, with accession number CGMCC No. 36747. This invention provides a formaldehyde-degrading bacterium that can tolerate high concentrations of formaldehyde and optimizes its degradation conditions to improve the treatment effect of high-concentration formaldehyde wastewater.

The invention belongs to the technical field of bioengineering, and discloses an expression strain of high-yield coenzyme Q10 and a production method of the coenzyme Q10. The expression strain of the high-yield coenzyme Q10, provided by the invention, is obtained by taking rhodobacter sphaeroides as an original strain and simultaneously carrying out gene modification construction of'crtI gene and crtB gene knockout 'and'GAP1 gene and / or UbiF gene overexpression'; through the combined action of multiple factors such as complete blocking of synthesis of carotenoid in cells, improvement of glycolysis efficiency, enhancement of reducing substance supply and enhancement of benzoquinone ring modification, directional reconstruction of metabolic flux for biosynthesis of the coenzyme Q10 in the rhodobacter sphaeroides cells is realized, so that the obtained expression strain is endowed with excellent coenzyme Q10 synthesis capability, and the expression efficiency of the rhodobacter sphaeroides is improved. Compared with an original strain, the yield of the coenzyme Q10 is increased, and the coenzyme Q10 has excellent application prospects in high-efficiency and low-cost industrial production of the coenzyme Q10.