12 results about "Rhodobacter" patented technology

The invention relates to the technical field of sewage treatment, discloses a sewage treatment dynamic load optimization method and system based on rhodobacter online monitoring, and aims to solve the problems of unstable process, effluent fluctuation, increase of energy consumption and material consumption and the like caused by lack of real-time accurate biological phase indication when the existing sewage treatment is used for coping with dynamic change of inflow load. According to the method and the system, biomass and physical and chemical parameters are obtained through online monitoring of rhodobacter and sewage parameters; after data preprocessing, dynamic load analysis is carried out to predict inflow water / biological load and a rhodobacter growth trend; the optimization decision module generates and controls the execution module to implement key operation parameter adjustment; and the man-machine interaction alarm module carries out visual management. According to the method, real-time quantitative evaluation of the biological phase is realized, the dynamic load adaptability, the operation efficiency and the effluent stability of the system are remarkably improved, and the energy consumption and the material consumption are reduced.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The application provides a preparation method of a Rhodobacter sphaeroides CsCobT and CsCobN protein complex in vitro, and steps are as follows: constructing an expression vector pET-28a(+)‑Cs CobN , pET-28a(+)‑SUMO‑Cs CobT ; expressing and purifying His-CsCobN protein; preparing a non-tagged CsCobT protein; mixing the purified His-CsCobN protein and the non-tagged CsCobT protein to form a protein complex; separating the complex through affinity chromatography; and refining the complex through gel filtration chromatography. The application adopts a strategy of double vectors, step-by-step purification, in-vitro assembly and multi-dimensional verification, can obtain a CsCobT and CsCobN protein complex with high purity and uniform conformation, and the experimental process can be accurately controlled, the result is specific, and provides high-quality protein materials for subsequent molecular mechanism research and application development of cobalt-chelating enzyme.

This invention provides a 5-aminolevulinic acid producing strain, its construction method, and its application. Based on the existing strain JAL9, it involves knocking out… pflB、ldhA、poxB、adhE、ackA、dppA Genes, upregulated eamA、grxA、sodB The transcriptional level of the katE gene was downregulated. pfkB Gene transcription level, heterologous expression hemA ST Genes and xfp The gene, along with a high-copy plasmid PETC4, expresses genes from... Rhodobacter capsulatus of hemA RC Genes, ultimately through hemB Introducing an ASV degradation tag at the gene terminus enhances the degradation of HemB protein, effectively improving the synthesis efficiency of 5-aminolevulinic acid. This method has good industrial application value when applied to the fermentation production of 5-aminolevulinic acid.

The application discloses a method for producing hydrogen through Clostridium pasteurianum-Rhodobacter sphaeroides coupling fermentation based on nitrogen limitation and application thereof. In the method, activated Clostridium pasteurianum is subjected to dark fermentation for producing hydrogen in a nitrogen-free culture medium and in an argon environment; then, the pH value of the obtained culture solution is adjusted to 7.0-8.0, the activated Rhodobacter sphaeroides is inoculated after selective sterilization, and the light fermentation for producing hydrogen is carried out in the argon environment. In the application, the fermentation solution of Clostridium pasteurianum does not need to be subjected to centrifugal removal of bacterial bodies and the like, and only needs to be simply adjusted in pH value, so that the light fermentation for producing hydrogen of Rhodobacter sphaeroides can be directly carried out. In the application, the nitrogen limitation is used to inhibit excessive proliferation of bacterial bodies, save carbon source flow, induce high expression of nitrogen fixation enzyme, and open a second high-efficiency hydrogen production path in addition to hydrogenase, so that the hydrogen production yield is further improved while the cost of nitrogen source is reduced. The hydrogen production yield of the application reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The application discloses a mutant strain with high coenzyme Q10 yield, and has a preservation number of CGMCC No.22200.The mutant strain is obtained by enrichment, separation, mutagenesis and screening of Rhodobacter sphaeroides with high coenzyme Q10 content.The application also discloses application of the mutant strain in production of coenzyme Q10 and in medicines, health products, cosmetics and food.

The application discloses a method for improving acid aluminum tolerance of sugarcane by high-efficiency combined nitrogen fixation, and relates to the technical field of sugarcane cultivation. The method comprises the following steps: controlling the nitrogen fertilizer amount, that is, only the bottom fertilizer applied in the whole growth period of the sugarcane contains the nitrogen fertilizer, and the fertilizer applied in other periods does not contain the nitrogen fertilizer; constructing a high-efficiency combined nitrogen fixation system, that is, applying the bacteria agent of Beijerinckia and the enhancer when the sugarcane is newly planted; applying the bacteria agent of Rhodobacter sphaeroides and the enhancer in the sugarcane seedling stage; applying the bacteria agent of Lipomyces and the enhancer in the sugarcane tillering stage; and the enhancer comprises indole acetic acid, eugenol and vitamin E. The method can make the sugarcane grow normally in the high-acid aluminum soil by controlling the nitrogen fertilizer amount, selecting suitable nitrogen fixation bacteria in different growth periods of the sugarcane and adopting a series of means such as the auxiliary use of the enhancer to construct the high-efficiency combined nitrogen fixation system.

This invention relates to the field of water treatment and discloses a strain of *Methylorubrum aminovorans* and its application in treating high-concentration formaldehyde wastewater. This *Methylorubrum aminovorans* strain is named 3-5-6, and its microbiological classification is *Methylorubrum aminovorans*. It was deposited at the China General Microbiological Culture Collection Center (CGMCC) on November 24, 2025, with accession number CGMCC No. 36747. This invention provides a formaldehyde-degrading bacterium that can tolerate high concentrations of formaldehyde and optimizes its degradation conditions to improve the treatment effect of high-concentration formaldehyde wastewater.

The invention belongs to the technical field of bioengineering, and discloses an expression strain of high-yield coenzyme Q10 and a production method of the coenzyme Q10. The expression strain of the high-yield coenzyme Q10, provided by the invention, is obtained by taking rhodobacter sphaeroides as an original strain and simultaneously carrying out gene modification construction of'crtI gene and crtB gene knockout 'and'GAP1 gene and / or UbiF gene overexpression'; through the combined action of multiple factors such as complete blocking of synthesis of carotenoid in cells, improvement of glycolysis efficiency, enhancement of reducing substance supply and enhancement of benzoquinone ring modification, directional reconstruction of metabolic flux for biosynthesis of the coenzyme Q10 in the rhodobacter sphaeroides cells is realized, so that the obtained expression strain is endowed with excellent coenzyme Q10 synthesis capability, and the expression efficiency of the rhodobacter sphaeroides is improved. Compared with an original strain, the yield of the coenzyme Q10 is increased, and the coenzyme Q10 has excellent application prospects in high-efficiency and low-cost industrial production of the coenzyme Q10.

The invention discloses application of rhodobryum harzianum in colorectal cancer diagnosis or prognosis, and belongs to the technical field of biomedicine.16SrRNA genome sequencing analysis finds that the content of rhodobryum harzianum in colorectal cancer tissue of a colorectal cancer patient is remarkably increased, the cancer promoting effect of the rhodobryum harzianum is further proved through a mouse AOM / DSS spontaneous tumor formation model, and the application of the rhodobryum harzianum in colorectal cancer diagnosis or prognosis is achieved. Compared with the prior art, the method provided by the invention has the advantages that the rhodobryum harzicola in a clinical sample is detected and a corresponding diagnosis model is established, so that the method has relatively high diagnosis efficiency and shows that the rhodobryum harzicola has important potential in clinical auxiliary diagnosis, treatment or prognosis evaluation of the colorectal cancer, a new way is provided for diagnosis, treatment or prognosis of the colorectal cancer, and the clinical application prospect is wide.

The present invention relates to a Rhodobacter cell engineered to utilize sucrose, comprising: (i) a heterologous enzyme having invertase activity; (ii) a heterologous enzyme having sucrose permease activity; and, optionally, (iii) a heterologous enzyme having fructokinase activity, and to a method for producing a Rhodobacter cell engineered to utilize sucrose. The invention further relates to a method for preparing a monoterpene, a sesquiterpene, a diterpene, a triterpene, a tetraterpene, a flavour, an aroma compound, an UV scavenger, a natural colorant, a natural crop protectant or an isoprenoid moiety containing compound such as coenzyme Q10, comprising culturing the Rhodobacter cell according to the invention in a culture medium comprising sucrose as carbon source for the monoterpene, sesquiterpene, diterpene, triterpene, tetraterpene, flavour, aroma compound, UV scavenger, natural colorant, natural crop protectant or the isoprenoid moiety containing compound such as coenzyme Q10. In addition, the invention pertains to the use of the Rhodobacter cell of the invention for the production of monoterpenes, sesquiterpenes, diterpenes, triterpenes, tetraterpenes, flavours, aroma compounds, UV scavengers, natural colorants, natural crop protectants or for production of isoprenoid moiety containing compounds such as coenzyme Q10.

The application discloses a recombinant saccharomyces cerevisiae for producing decaprenylphenol and a construction method and application thereof, and belongs to the technical field of microorganisms. Rhodobacter sphaeroides The recombinant saccharomyces cerevisiae is obtained by expressing a decaprenyl pyrophosphate synthase gene from Rhodobacter sphaeroides in the saccharomyces cerevisiae, overexpressing a phospholipase gene of endogenous phosphatidic acid of the saccharomyces cerevisiae, and down-regulating a transcription level of a squalene synthesis gene ERG9 and strengthening an endogenous MVA pathway. 10 The application realizes the production of decaprenylphenol in the saccharomyces cerevisiae for the first time, and the yield reaches 112 mg / L in a flask; decaprenylphenol is extracted from the recombinant saccharomyces cerevisiae through fermentation, and coenzyme Q 10 is synthesized through a chemical method, compared with traditional fermentation of Rhodobacter sphaeroides for producing coenzyme Q 10 , the method has the advantages of high fermentation stability, simple culture medium and the like, and has industrial application potential.