Rhodobacter sphaeroides extracting solution with antioxidant activity and preparation method thereof

A technology of Rhodobacter sphaeroides and antioxidant activity, applied in biochemical equipment and methods, microbial-based methods, redox enzymes, etc., can solve problems that have not been reported

Active Publication Date: 2014-10-15
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There is no report on the application of nanotechnolog

Method used

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  • Rhodobacter sphaeroides extracting solution with antioxidant activity and preparation method thereof
  • Rhodobacter sphaeroides extracting solution with antioxidant activity and preparation method thereof
  • Rhodobacter sphaeroides extracting solution with antioxidant activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Calculation of carotenoid content

[0087] Measure the absorbance of the extracted carotenoid-containing acetone solution at 475nm; refer to Huibodi et al. 29(12):587-591.

[0088] ] method to calculate the carotenoid content. Calculated as follows:

[0089]

[0090] A—absorbance value at 480nm;

[0091] y—the volume of the sample solution (mL);

[0092] -Absorptance coefficient, defined as the theoretical absorption value of a solute with a concentration of 1% (W / V) in a cuvette with an optical path length of 1 cm, and a value of 2500 is adopted here.

[0093] Determination of the ability of carotenoids to scavenge DPPH free radicals in Rhodobacter sphaeroides [Liu Wei, Liu Yanxia, ​​Zhao Jian, et al. Feasibility study of DPPH method to determine the antioxidant capacity of fat-soluble ingredients in health food [J]. China Brewing, 2011, 04:86-89. and Hongfei Fu, Bijun Xie, Shaojun Ma, Xinrong Zhu, Gang Fan, Siyi Pan. Evaluation of antioxidant activities of p...

Embodiment 2

[0159] Determination of SOD content

[0160] Determination was carried out according to the instructions of the total antioxidant capacity test box built in Nanjing.

[0161] Determination of the ability of Rhodobacter sphaeroides SOD to scavenge ABTS free radicals [see Hongfei Fu, Bijun Xie, Shaojun Ma, Xinrong Zhu, Gang Fan, Siyi Pan. Evaluation of antioxidant activities of principal carotenoids available in water spinach (Ipomoea aquatica). Food Composition and Analysis, 2011, 24:288–297.

[0162] Perumal Siddhuraju. The antioxidant activity and free radical-scavenging capacity of phenols of raw and dry heated moth bean (Vigna aconitifolia) (Jacq.) Marechal seed extracts. Food Chemistry, 2006,99:149–157.

[0163] Liu Wei, Qiu Le, Yang Jing, Liu Yanxia, ​​Zhao Jian, Wen Jing, et al. Comparative analysis of ABTS and phenanthroline-Fe3+ method for determination of antioxidant capacity of health food[J]. Food Industry, 2013,34(3), 120-123.

[0164] Andrzej L. Dawidowicz, Mal...

Embodiment 3

[0228] coenzyme Q 10 determination

[0229] High performance liquid chromatography was used to determine the content of coenzyme Q10 in the coenzyme Q10 extract according to "Determination of Coenzyme Q10 in Health Food" (GB / T22252-2008).

[0230]Determination of Scavenging DPPH Free Radical Ability of Rhodobacter sphaeroides Coenzyme Q10

[0231] Concrete operation is with embodiment 1.

[0232] Determination of Anti-lipid Peroxidation Ability of Rhodobacter sphaeroides Coenzyme Q10

[0233] Concrete operation is with embodiment 1.

[0234] Determination of Reducing Power of Rhodobacter sphaeroides Coenzyme Q10

[0235] Concrete operation is with embodiment 1.

[0236] The preparation steps of Rhodobacter sphaeroides coenzyme Q10 extract:

[0237] (1) Activation of bacteria

[0238] Prepare solid medium (mass percentage, the same below): glucose 2%, tryptone 1%, yeast extract powder 1%, sodium chloride 0.5%, agar 2%, pH=8.5; Sterilize by high-pressure steam at 121°C f...

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Abstract

The invention discloses a rhodobacter sphaeroides extracting solution with antioxidant activity and a preparation method thereof and belongs to the technical field of rhodobacter sphaeroides extracting solutions. The extracting solution contains carotenoids or a coenzyme Q10 or an SOD (superoxide dismutase). The preparation method comprises the steps as follows: activating strains, fermenting rhodobacter sphaeroides, performing centrifugal separation on the rhodobacter sphaeroides and preparing the rhodobacter sphaeroides extracting solution. The prepared rhodobacter sphaeroides extracting solution has the groundbreaking significance.

Description

technical field [0001] The invention relates to a method for preparing an extract of Rhodobacter sphaeroides with antioxidant activity, and belongs to the technical field of extracts of Rhodobacter sphaeroids. Background technique [0002] Carotenoids are provitamin A, which have the functions of scavenging free radicals, anti-oxidation, enhancing immunity, and preventing cancer. They are widely used as colorants and functional ingredients in food, medicine, and cosmetics. restricted. The vast majority of commercially available carotenoids are derived from chemical synthesis, which cannot meet consumers' demand for natural carotenoids, so the research focus has shifted from chemical synthesis to biosynthesis. Microbial biosynthesis of commercial bioactive pigment carotenoids has attracted extensive attention due to its high efficiency and ease of manipulation. [0003] Coenzyme Q10 is a fat-soluble vitamin-like substance that widely exists in the mitochondria of biological...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12P7/66C12N9/02C12R1/01
Inventor 李祖明安君惠博棣高丽萍白志辉王栋杨卫东
Owner BEIJING UNION UNIVERSITY
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