Method for reducing or eliminating by-product D in coenzyme Q10 producing strain SZ, coenzyme Q10 high-yield strain and application thereof

Abstract

The invention discloses a method for reducing or eliminating a by-product D in a coenzyme Q10 producing strain SZ. According to the method, the gene of 5-demethoxy panthenol hydroxylase is expressed in the coenzyme Q10 producing strain SZ, so that the accumulation of the by-product D is reduced or eliminated, wherein the producing strain SZ is classified and named as rhodobacter sphaeroides, which is preserved in China General Microbiological Culture Collection Center on March 3, 2016, with preservation number of CGMCC NO. 12177. The invention also discloses a coenzyme Q10 high-yield strain SF and an application thereof, wherein the high-yield strain SF is classified and named as rhodobacter sphaeroides, which is preserved in China General Microbiological Culture Collection Center on March 3, 2016, with preservation number of CGMCC NO. 12178. The high-yield strain SF is obtained by expressing the gene of the 5-demethoxy panthenol hydroxylase in the producing strain SZ; and with the application of the high-yield strain SF, the yield of produced coenzyme Q10 reaches 2g / L or above; therefore, the high-yield strain SF has a broad application prospect.

Description

technical field [0001] The invention relates to the technical field of modern biology, in particular to a 10 A method for reducing or eliminating by-product D in the production strain SZ, a coenzyme Q10 high-yielding strain and applications thereof. Background technique [0002] coenzyme Q 10 (Coenzyme Q 10 , CoQ 10 ), the chemical name is: 2,3-dimethoxy-5-methyl 6-decisopentenylbenzoquinone, and the molecular formula is C 59 h 90 o 4 , the relative molecular weight is 863, the melting point is 48-50 ℃, it is orange-yellow crystal at room temperature, odorless and tasteless. Soluble in chloroform, benzene, carbon tetrachloride, soluble in acetone, petroleum ether and ether, slightly soluble in ethanol, insoluble in water and methanol. It is easy to decompose into reddish under light, and is relatively stable to temperature and humidity. [0003] coenzyme Q 10 It is a fat-soluble quinone compound, which was first discovered in 1957. Due to its existence in human orga...

AI Technical Summary

Problems solved by technology

The three methods have their own advantages and disadvantages. The animal and plant tissue extraction method restricts the production of coenzyme Q due to the supply cycle of raw materials and the source of the source. 10 mass production of

One of the problems often encountered in the modern biological breeding of traditionally bred microorganisms used for industrial fermentation is that conventional DNA transformation techniques for wild-type strains have a negative effect on those traditional breeding (such as chemical, The high-yield strains obtained by radiation mutagenesis) have no effect, so the corresponding microorganisms cannot be genetically engineered, such as gene expression, gene removal, etc.

coenzyme Q 10 The wild-type strain of Rhodobacter sphaeroides can generally be transformed by conjugation and electroporation (Piekarski, T. et al BMC Microbiology (2009) 9:265), but in practice the DNA extracted from E. Two Ways Coenzyme Q 10 The production strain cannot achieve any transformation

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