4results about How to "Stable genetic traits" patented technology

The invention relates to the field of gene engineering, and discloses a construction method and application of a GPX4S131A mutant endothelial cell line. According to the invention, the CRISPR / Cas9 technology is utilized to construct a gRNA vector containing targeted GPX4, ssODN is utilized to carry out targeted alanine (A) mutation on serine (S) of the 131 basic group of GPX4, and a stable GPX4S131A mutant endothelial cell ferroptosis model is constructed, so that a specific mechanism of endothelial cell ferroptosis is disclosed, a useful clue is provided for treating cardiovascular diseases caused by endothelial cell ferroptosis, and the application has a wide application prospect. The important application value is realized.

This invention provides a rice gene Bph54 for resistance to brown planthopper and its application. The rice provided by this invention... Bph54 Genes are of great significance for breeding new rice varieties resistant to brown planthoppers. This invention successfully cloned the rice Bph54 gene using map-based cloning, and performed functional annotation and identification. This gene exhibits complete dominance, and both homozygous and heterozygous genotypes confer equal resistance to brown planthoppers in rice. This characteristic is beneficial for the application of this gene in breeding. Based on this, the Bph54 gene can be introduced into brown planthopper-susceptible plant varieties through traditional breeding techniques such as hybridization and backcrossing, or through genetic engineering, to cultivate new varieties of plants resistant to brown planthoppers with stable genetic traits. This invention provides important germplasm resources for effectively reducing insect damage and achieving increased and stable crop yields.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

The application discloses a kind of pyricularia oryzae antagonistic streptomyces fermentation preparation, preparation method and application, belong to microbial fermentation and biological pesticide field, which comprises the following steps: screening the inhibitory rate of more than 80% of pyricularia oryzae starting strain of streptomyces, ribosome engineering is reformed by rpsL gene K88E directional point mutation, the value is greater than 5000 ug / mL in the optimized fermentation medium of carbon-nitrogen ratio 25:1~30:1, corn syrup 0.5%~1% culture makes potency, and the preparation of suspension preparation containing viable bacteria and metabolites is prepared by suspending liquid and supernatant concentrate compound, and the comprehensive prevention and cure effect of the preparation in field reaches more than 75%, which can replace chemical pesticide.