Rhodobacter strain and application thereof

A technology of red bacteria and strains, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of agricultural product export impact, affecting the quality of agricultural products, pesticide residues exceeding the standard, etc., achieve good pesticide removal effect, low production cost, and promote The effect of crop growth

Active Publication Date: 2010-06-09
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, while chemical pesticides kill pests, they also kill beneficial organisms and remain in the ecosystem, causing serious harm to the ecosystem
Extensive use of chemical pesticides can also easily lead to exc

Method used

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  • Rhodobacter strain and application thereof
  • Rhodobacter strain and application thereof

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Experimental program
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Effect test

Embodiment 1

[0025] A rhodobacter strain of the present invention, which is preserved in China Center for Type Culture Collection CCTCC, and the preservation number is CCTCC M 209239. This strain has the following main characteristics:

[0026] G-, the individual shape is rod-shaped, V-P reaction is negative, methyl red reaction is negative, starch cannot be utilized, H 2 S reaction is negative, cannot grow in 3% NaCl, the optimum growth temperature is 30°C-35°C, the optimum growth pH value is 6.5-7.5, the characteristic absorption peaks of the bacteria are 525nm, 805nm and 865nm, and the length of the 16S rDNA sequence is 1416bp, the accession number in Genbank is GQ429219.

[0027] In this embodiment, the above-mentioned rhodobacter CCTCC M 209239 is used to prepare a pyrethroid pesticide degrading bacterial agent, and the method specifically includes the following steps:

[0028] (1) Activation: the cryopreserved species of the above-mentioned rhodobacter CCTCC M 209239 is cultivated by...

Embodiment 2

[0034] Application of bacterial strain of the present invention in degradation medium with high concentration of fenpropathrin

[0035] Fenpropathrin is dissolved in acetone, added to the final concentration of fenpropathrin in the experimental medium is 600mg / L, and the experimental medium used is: (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.02%, NaHCO 3 0.5%, K 2 HPO 4 0.05%, NaCl 0.02% and yeast extract 0.15%, the pH value of this experimental medium is 7.0~7.2, the temperature is 30 ℃~35 ℃, and the light intensity is 2000Lx~3000Lx, the inoculum prepared in the embodiment 1 is inoculated into The experimental medium was cultured for 15 days. The fenpropathrin in the culture medium was extracted with petroleum ether, and the residue of fenpropathrin was determined by gas chromatography (Agilent 6890N), and the detector was an electron capture device (ECD). The experimental medium without adding bacteria was used as the control. All treatments were repeated three times, and the mea...

Embodiment 3

[0040] Application of bacterial strain of the present invention in degrading field cabbage and cypermethrin in soil

[0041] Select 4.5% beta-cypermethrin emulsifiable concentrate (produced by Nanjing Red Sun Group Co., Ltd.) as the medicament, the recommended dose is 1.5 times the upper limit, and the spraying is calculated by spraying 60kg of water per mu. The area of ​​the medicament treatment area is 32.25m 2 , the area of ​​the control plot is 80.75m 2 According to the recommended harvest interval (14 days) and the interval (10 days) between two sprays at the time of registration, it is determined to spray three times. The first spraying time is August 31, the second is September 10, and the third is September 20.

[0042] Get the bacterium agent 200ml that embodiment 1 prepares, evenly spray on the Chinese cabbage (Xiayangbai) after watering 60kg. The time of spraying the bacteria agent for the first time was August 31, the second time was September 10, and the third t...

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Abstract

The invention provides rhodobacter strain and application thereof. The rhodobacter strain is collected in the China Center for Type Culture Collection (CCTCC), and the collection number of the rhodobacter strain is CCTCC No: M 20239. The rhodobacter strain can be used for preparing a pyrethroids pesticide degradation fungicide and has the advantages of low production cost, good pesticide removal effect, high efficiency, no toxicity, environmental protection, and the like. The rhodobacter strain can prepare the pyrethroids pesticide degradation fungicide through a simple, quick and low cost operation method.

Description

technical field [0001] The invention relates to a bacterium and its application, in particular to a bacterial strain and its application in degrading pesticide residues. Background technique [0002] The use of chemical pesticides has saved a large amount of losses caused by insect pests for agricultural production, and has greatly promoted the development of modern agricultural production. However, while chemical pesticides kill pests, they also kill beneficial organisms and remain in the ecosystem, causing serious harm to the ecosystem. Extensive use of chemical pesticides can easily lead to excessive pesticide residues in agricultural products, seriously affecting the quality of agricultural products, posing a serious threat to people's health, and negatively affecting the export of agricultural products. [0003] Pyrethroid insecticides are a class of synthetic compounds that resemble natural pyrethrins. Since the 1980s, it has been widely used in various crops such as...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02A62D101/28A62D101/04C12R1/01
Inventor 刘勇张松柏张德咏罗香文成飞雪罗源华程菊娥何明远谭新球戴建平朱春晖杨春晓刘绍文
Owner HUNAN PLANT PROTECTION INST
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