104results about How to "Large amount of synthesis" patented technology

The preparation method of girasole beverage includes three technological process steps: 1. preparation of girasole hydrolyzate; 2. preparation of osmanthus flower extract; and 3. blending, packaging and sterilizing. The girasole hydrolyzate, osmanthus flower extract, phosphoric acid and citric acid are mixed according to the mixing ratio of 100:(10-12):3:(4-6), then water is added according to the ratio of the above-mentioned mixed material liquor and water of 1:(50-60), uniformly stirred, bottled, capped and sterilized so as to obtain the invented product with rice nutrients and several health-care functions of reproducing bifidobacteria in human body, inhibiting the growth of intestinal salmonella and spilage organism, raising immunity of human body and reducing blood-lipid, etc.

The invention discloses a glutathione producing strain, in which a GSH related synthetic enzyme Gamma-L-glutamyl-L-cysteine synthetic enzyme gene GSH I, a glutathione synthetic enzyme gene GSH II, and a cysteine related synthetic enzyme cystathionine synthetic enzyme gene CYS3 and a cystathionine-Gamma-lyase CYS4 gene are introduced. The glutathione producing strain of the invention has the advantages of high density and low cost.

The invention relates to a method for preparing cupric tungstate via a cation membrane electrolysis method. The method comprises the following steps: carrying out constant-current electrolysis or constant-voltage electrolysis on a sodium tungstate solution by utilizing a cation membrane for 3 to 30 minutes by taking a copper sheet as an anode, a noble electrode as a cathode, a sodium tungstate solution as an anolyte and a solution with pH between 0 and 14 as a catholyte under the condition that the temperature of the electrolytes is raised from room temperature to 90 DEG C; then cleaning an obtained product and drying; carrying out high-temperature roasting on the dried product, namely putting the product into a container under an air atmosphere, heating to 450 DEG C to 600 DEG C under a heating rate of 2.5 to 10 DEG C/min and roasting at a constant temperature for 1 to 5 hours; and naturally cooling to the room temperature, thereby obtaining a pure cupric tungstate crystal. According to the method, the Na<+> is transferred into a cathode chamber under the action of the electrolysis of the cation membrane; the reaction time is short and any additives are not used, so that the pure cupric tungstate can be obtained; and any impurities and impurity ions are not generated, so that the subsequent treatment is simple. In addition, the method is simple in process, easy to operate, small in investment and large in synthesis quantity. Thus, the method can be directly applied to industrial production.

The invention provides a Chinese herbal medicinal feed additive for improving the quality of carp flesh, and the Chinese herbal medicinal feed additive is prepared from the following raw material drugs in parts by weight: 2 to 4 parts of eucommia, 2 to 4 parts of dandelion, 3 to 5 parts of angelica root, 3 to 5 parts of yam, and 3 to 5 parts of chrysanthemum. A preparation method of the additive comprises the following steps: weighing the eucommia, the dandelion, the angelica root, the yam and the chrysanthemum Chinese herbal medicinal slices in weight proportion, and respectively crushing into coarse powder; collecting volatile oil from the chrysanthemum coarse powder by using a distillation method; immersing the eucommia, dandelion, angelica root and yam Chinese herbal medicinal coarse powders with water, and decocting for three times with the distilled chrysanthemum dreg with slow fire; combining the three filtrates, and heating to obtain concentrated liquid; and combining the volatile oil and the concentrated liquid, adding antiseptic and water quantitatively, uniformly stirring, filtering, filling and sterilizing to obtain the Chinese herbal medicinal feed additive. The Chinese herbal medicinal feed additive is used for improving the quality of carp flesh, and has the advantages of convenient use, low cost, good effect, safety and non toxic or side effect.

The invention relates to a universal method for controllably preparing nano particles on a graphene sheet. The method comprises the steps of selecting a graphite oxide colloidal aqueous solution and a high-purity Ag target, suspending the Ag target in the graphite oxide colloidal solution, ceaselessly mixing the colloidal aqueous solution with a magnetic mixer, irradiating the target with a laser, and regulating the density and dimensions of the Ag nano particles by changing irradiation time and laser power. The method overcomes the defects that the application of graphene or graphite oxide is limited due to nonuniform distribution of the nano particles prepared by a chemical method on the sheet, additives are required to result in sample contamination, instruments are expensive, the preparation cost is high, and large-scale preparation cannot be achieved when a physical method is used for preparing a graphene based composite. The invention provides a liquid laser ablation method, The graphene based composite can be prepared no matter functional groups are available on the graphene sheet, a technology is simple, the synthetic quantity is large, and the method is green and environment-friendly and can be popularized and used in the industrial field.

The invention provides a carrier for enhancing aluminum-tolerance of a plant, and a method for establishing the same. The carrier is a plant expression vector having photoinduction promoters and phosphoenolpyruvate carboxylase (PEPC) genes. The method for establishing the carrier comprises the following steps: searching for the sequence of the full length gene of Synechococcus vulcanus PEPC in GenBank and designing a pair of primers with sequences as described in the specification; recovering and purifying PEPC full length gene segments and connecting the segments to a pUCm-T vector; establishing an entry vector pENTER*-PrbcS-PEPC; establishing a plant expression vector pH2-35S-PrbcS-PEPC. In the invention, the activity of citrate synthase of tabacoo with transgenic PEPC and CS genes is 2.4 to 2.6 times that of wild tobacco, and the activity of phosphoenolpyruvate carboxylase of such tabacco is 2.2 to 2.4 times that of wild tobacco. The special-purpose carrier provided in the invention can exert great influence on the improvement of aluminum-tolerance of a plant, and particularly, can significantly promote aluminum-tolerance of plants grown in acid red soil in southern China, thereby providing a novel approach for variety improvement of plants.

The invention discloses a strain of acinetobacterbeijerinckii and application of the acinetobacterbeijerinckii. The acinetobacterbeijerinckii LJL-12 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6291. The acinetobacterbeijerinckii LJL-12 has the following application values that the acinetobacterbeijerinckii LJL-12 can grow in the environment adopting ACC (aminocyclopropane carboxylic acid) as the unique nitrogen source, and meanwhile, the ACC is decomposed; IAA (indole acetic acid) can be synthesized, and in addition, the IAA synthesis quantity is increased along with the concentration increase of L-Trp; siderophores can be synthesized; and the promotion effect is realized on the oat growth in petroleum polluted saline-alkali soil. The acinetobacterbeijerinckii LJL-12 is hopeful to realize the important effect in the petroleum polluted saline-alkali soil plant restoration.

The invention provides a method for preparing zinc tungstate by using a cationic membrane electrolysis process. The method comprises the steps of utilizing a zinc sheet as an anode, a noble electrode as a cathode, an aqueous solution containing sodium tungstate as an anode liquor, and an acid/alkali/salt solution as a cathode liquor; in a double-compartment electrolytic cell with a cationic membrane as a diaphragm, controlling the temperature from room temperature to 90 DEG C to electrolyze the aqueous solution containing sodium tungstate in a constant-current electrolysis or constant-voltage electrolysis manner till all Na<+> ions are transferred to a cathode compartment; simultaneously cleaning and filtering products from the anode by using deionized water to obtain a filter cake, drying the filter cake, and then increasing the temperature to 450-600 DEG C under air atmosphere at the speed of 2.5-10 DEG C/min to perform high-temperature roasting on the dried filter cake for 1-5 hours; naturally cooling the roasted product to the room temperature, and then obtaining the zinc tungstate with high purity and no impure phase. According to the method for preparing the zinc tungstate by using the cationic membrane electrolysis process, all the Na<+> ions are transferred to the cathode compartment under the action of the cationic membrane electrolysis, so that the Na<+> influence is eliminated and the reaction time is short.

The invention discloses a preparation method of Schweetmannite based on ZVI reduction coupling microorganisms, and the method comprises the following steps: (1) mixing a bacterial liquid obtained by strain cultivation with FeSO4.7H20, stirring and dissolving to obtain a mixed liquid; (2) aerating and oxygenating the mixed liquid obtained in the step (1) in a vessel 1 until Fe<2+> is completely oxidized to Fe<3+>, transferring a part of the mixed liquid to another vessel 2, and adding ZVI for mixing and stirring for performing Fe<3+> reduction; (3) after Fe<3+> reduction for a certain period oftime in the step (2), performing solid-liquid separation on the ZVI and a reduced liquid mixture, and transferring the separated liquid mixture back to the vessel 1 for aeration and oxygenation; and(4) performing circulation according to the steps (2) and (3) until total iron TFe in the whole system precipitates completely, collecting by filtration a mineral synthesized in the vessel 1, washingwith water and drying the mineral to obtain the Schweetmannite. The method not only improves the iron utilization rate and the total iron precipitation rate, but also continuously produces the Schweetmannite.

The invention discloses a polypeptide composition with an effect of promoting melanogenesis and application thereof, and belongs to the technical field of cosmetic polypeptides. The composition comprises alpha-MSH bionic peptide and a pre-melanogenesis agent. The polypeptide composition can also be added with a polypeptide capable of improving the cAMP level and/or an active component for promoting tyrosinase gene expression and/or an active component with an antioxidant effect and/or a polypeptide with a hair growth promoting effect. The polypeptide composition disclosed by the invention actson different targets through compounding of different active ingredients, comprehensively plays a synergistic effect, remarkably promotes melanogenesis, and can be used for turning white hair into black hair, turning skin into brown and/or preventing damage caused by ultraviolet irradiation.

The present invention discloses a high-new bio-technology, and particularly relates to a Corynebacterium glutamicum DAHP synthase gene cloning and L183Q site directed mutagenesis method, wherein an enzyme engineering technology and a molecular biology technology are adopted, and combined with a metabolic engineering theory, a fermentation engineering technology and other high-new bio-technologies, a substrate structure analogue resistance mutant and phenylalanine-tyrosine double auxotrophic Corynebacterium glutamicum are adopted as starting strains, researches on Corynebacterium glutamicum self-feedback regulation release, metabolic flow enhancement, improvement of catalysis activity of key enzymes in a tryptophan biosynthesis path, and substrate biosynthesis increase are performed respectively according to the metabolic engineering theory, and a tryptophan biological metabolism path of the Corynebacterium glutmicum is reasonably designed and modified to obtain the gene engineering bacterial, wherein the gene engineering bacterial has the following characteristics that: acid production rate and conversion rate meet contract requirements, and corn is adopted as a raw material to produce feed grade L-tryptophan.

The preparation process of malaytea scurfpea injection includes the following steps: eliminating impurity from malaytea scurfpea fruit as material, crushing into coarse powder, ultrasonic extraction in 75 % concentration alcohol solution for 2-4 times of 1-2 hr each to obtain the extracted liquid, decompression concentration to obtain dense paste while recovering alcohol, diluting with 0.85 % concentration sodium chloride solution via stirring, letting stand and taking supernatant, regulating pH to 3.0 with hydrochloric acid, cold storing, regulating pH to 6.5-7.5, filtering, filling, sterilizing and packing the malaytea scurfpea injection. The malaytea scurfpea injection is used in intramuscular injection to treat leucoderma and psoriasis and can heal leucoderma and psoriasis radically.

The invention discloses uses of a histidine kinase gene Hisk2301, particularly applications of a histidine kinase gene Hisk2301 in production of polyunsaturated fatty acids at a low temperature. According to the present invention, polyunsaturated fatty acids are produced at a low temperature with the histidine kinase gene Hisk2301, and the contents of the polyunsaturated fatty acids such as LA and ALA are improved by modifying microorganisms through genetic engineering, such that the good application prospects and economic benefits are provided for the industrial production of polyunsaturated fatty acids, and the foundation is established for the large-scale industrial production of polyunsaturated fatty acids.

The invention relates to panax notoginseng massage cream and a preparation method thereof and belongs to the field of cosmetics. The panax notoginseng massage cream comprises raw traditional Chinese medicinal materials in percentage by weight as follows: 15%-22% of panax notoginseng volatile oil, 10%-18% of a radix rolygoni multiflori extract, 14%-16% of a hawthorn fruit extraction solution, 8%-10% of a green tea extract, 8%-10% of sorbitol, 8%-10% of white oil, 3%-6% of polyoxyethylene dodecanol ether, 0.1%-0.3% of Tween-60, 0.1%-0.3% of methylparaben and the balance of purified water. According to the massage cream, the panax notoginseng volatile oil from a precious medicinal material, namely, panax notoginseng, is taken as the main raw material, the fleeceflower root extract, the hawthorn fruit extraction solution, the green tea extract and the like are added for reasonable matching, monarch and ministerial drugs are compatible and act in a combined manner, and the massage cream has effects of clearing facial grease, minimizing pores, eliminating facial edema and resisting skin ageing, has better prevention and treatment effects on milium, fades freckles, promotes absorption of nutritional substances and enables the skin to be ruddy and tight.

The invention discloses a preparation method of a straw micro-storage feed, and is characterized in that the preparation method includes the following steps: (1) constructing a micro-storage cellar; (2) carrying out raw material pretreatment comprising sun-drying straw for 3-5 h to make the water content be 60%-70%, and cutting short into 2-3 cm; (3) preparing a fermentation agent: (4) weighing table salt according to 0.3% (weight percentage) of the straw dry basis amount, and evenly mixing into a fermentation bacterial powder; (5) laying a plastic film in the cellar, allowing the plastic film length to be 1-1.5 m higher than that the periphery of a cellar mouth; (6) sprinkling the crushed straw with the thickness of 20-30 cm at the bottom of the cellar, then sprinkling the prepared fermentation bacterial powder on the straw, and treading down with feet; and (7) allowing the micro-storage anaerobic fermentation time to be 7-8 days in summer, 10-12 days in spring and in autumn and 13-15 days in winter, checking whether the feed has a wine fragrance, and allowing for use. The nutritional value of the straw feed can be improved, and the feed has relatively high content of digestible proteins and has good palatability.

The invention discloses a preparation method of vanadic acid copper. The preparation method comprises the steps that firstly, by the adoption of the direct current electrolysis mode, vanadic acid copper precursors of different structures and with different particle sizes are prepared by controlling the current density, electrolyte concentration and temperature; then products obtained in anode liquor are collected, filtered, washed and dried; and finally, the temperature is increased to 400-600 DEG C at the speed of 2-5 DEG C/min in the air atmosphere, high-temperature roasting is conducted for 3-5 h, and the high-purity and high-cleanliness vanadic acid copper is obtained, specifically, the structural composition of the vanadic acid copper is Cu<3>(VO<4>)<2>. According to the preparation method, by controlling the corresponding conditions, main morphological structures of the obtained product are spherical particles and micron rod-like structures.

The invention provides a method for preparing a wave-absorbing material by means of nickel slag, and belongs to the technical field of metallurgy. The method comprises the following preparation stepsthat after the nickel slag and calcium oxide are mixed, tabletting is conducted to obtain modified nickel slag; the obtained modified nickel slag is subjected to fusion oxidation, and an non-full particle magnetite phase is separated out, so that oxidized modified nickel slag is obtained; the oxidized modified nickel slag is subjected to breaking and magnetic separation in sequence to obtain a magnetic substance; the obtained magnetic substance is ground into 70-150 nm, so that the wave-absorbing material is obtained. According to the method for preparing the wave-absorbing material by means of the nickel slag, the nickel slag is treated through a fusion oxidation method to make iron in the nickel slag be enriched to the magnetite phase selectively, moreover, Fe3O4 nanocrystals are embedded into multiple kinds of oxides, after magnetic separation, a small amount of dielectric oxide SiO2 still exists, aggregation of Fe3O4 particles due to magnetism is avoided, the synthetic amount is large, and large-scale production can be achieved.

The invention pertains to the field of nanomotors, and in particular relates to a mesoporous-macroporous nanomotor as well as a preparation method and application thereof. The mesoporous-macroporous nanomotor is of a hierarchical pore structure and comprises a mesoporous silica core, a macroporous layer and a metal platinum layer, wherein the surface of the mesoporous silica core is covered with the macroporous layer and the metal platinum layer. The preparation method comprises the following steps: synthesis of the mesoporous silica core, synthesis of mesoporous-macroporous silica and introduction of platinum nanoparticles on a macroporous surface so as to obtain the mesoporous-macroporous nanomotor. The mesoporous-macroporous nanomotor has the characteristic of double-layer structure, has the function of loading drugs in a hierarchical manner and is high in biocompatibility. The preparation method disclosed by the invention is simple, mild in synthesis conditions, and high in one-time synthesis capacity, and therefore, the preparation method has the broad application prospect in the biomedical field.

Disclosed are bacillus thuringiensis with high insecticidal performance and environmental friendliness and application thereof. The bacillus thuringiensis with high insecticidal performance and environmental friendliness is bacillus thuringiensis delta leuB of which the collection number is CCTCC NO: M 2019112. A gene leuB in the genome of the bacillus thuringiensis Bt 4.0718 is successfully knocked out by an I-SceI mediated traceless gene knockout system to obtain a mutant strain of the bacillus thuringiensis; the phenotypic change of the mutant strain delta leuB is obvious, no mature germs are found in an LB liquid culture medium, the synthetic amount of crystal protein is increased, metrocytes are not broken, and the crystal protein is wrapped into a microcapsule; the mutant strain hashigher insecticidal activity on plutella xylostella than wild strains, and the differences are remarkable.

The invention relates to a Pseudomonas aeruginosa for improving the yield of rhamnolipid, and a construction method thereof. The gene expression level of group A of the above strain is decreased or lost while the gene expression level of group B of the strain is increased; gene in the group A is one selected from a pslABCDEFGHIJKLMNO extracellular polysaccharide synthetic gene cluster, a pelABCDEFG polysaccharide synthetic gene cluster, an alginate synthetic gene cluster algD-alg8-alg44-algKEGXLIJF and a polyhydroxy fatty acid PHA synthetic key gene cluster phaC1-D-C2, or an arbitrary combination of the genes; and gene in the group B is one selected from rmlACBD, rmlBDAC, rhlYZ, algC, fadD4, lipC and estA, or an arbitrary combination of the genes. The Pseudomonas aeruginosa has the advantages of significant improvement of the yield of the rhamnolipid, stability in inheriting, and facilitation of the separation and purification of downstream products.

The invention provides a method for preparing a wave absorbing material from copper slags, and belongs to the technical field of metallurgy. The method comprises the following preparation steps: mixing copper slags with calcium oxide and then performing tabletting to obtain modified copper slags; performing melting-oxidation on the obtained modified copper slags to obtain oxidation modified copperslags, and separating out a non-full granular magnetite phase; performing crushing and magnetic separation on the oxidation modified copper slags in sequence to obtain a magnetic substance; and grinding the obtained magnetic substance to 70 to 150 nm to obtain the wave absorbing material. According to the method provided by the invention, the copper slags are treated by using the melting-oxidation method, so as to enable iron components in the copper slags to selectively enrich with the magnetite phase, and Fe3O4 nanocrystals are embedded in various oxides, and a small quantity of oxide SiO2with dielectric properties is left still after the magnetic separation, the agglomeration of Fe3O4 particles caused by magnetic properties can be avoided, the synthesis capacity is large, and the massproduction can be realized.