Method for producing docosahexaenoic acid by using schizochytrium limacinum
A technology of docosahexaenoic acid and Schizochytrium, which is applied in the field of fermentation, can solve the problems of increasing income and high energy consumption, and achieve the effects of increasing biomass, increasing production, and optimizing process parameters
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Embodiment 1
[0044] A method utilizing Schizochytrium to produce docosahexaenoic acid, comprising the following steps:
[0045] (1) Activate and cultivate Schizochytrium sp. S056 into Schizochytrium sp. seed liquid.
[0046] (2) Put the Schizochytrium seed solution into the fermenter according to the volume ratio of 2% for cultivation, the cultivation temperature is 28 degrees Celsius, ventilate and keep stirring at a constant speed, the ventilation volume is 10 cubic meters per hour to 50 cubic meters per hour, and at the same time, the flow is supplemented feed, the feed includes carbon source, nitrogen source and glycerin, and maintains the concentration of glycerol at 0 to 10 g / L. The carbon source is glucose, and the nitrogen source is yeast powder. Feed feeding is divided into three times, each feeding, when the carbon source concentration in the fermentation broth drops to 20.0 g / L to 30.0 g / L, start feeding, when the carbon source concentration in the fermentation broth is 30.0 g ...
Embodiment 2
[0056] A method utilizing Schizochytrium to produce docosahexaenoic acid, comprising the following steps:
[0057] (1) Activating and culturing Schizochytrium to produce Schizochytrium seed liquid. The Schizochytrium is obtained according to the following method: take a certain amount of water sample and apply it to a high-salt YPD solid medium (glucose 20.0g / L, yeast powder 10.0g / L, peptone 20.0g / L , agar 20.0g / L, sea crystal 10.0 g / L), cultured at 25.0 degrees Celsius for 48 hours, a large number of single colonies were obtained, picked single colonies in liquid medium, and cultured on a shaker at a speed of 180 rpm and a culture temperature of 25.0 degrees Celsius After 72 hours of shaking flask culture under certain conditions, the bacteria were stained and observed under a microscope to screen out the strains with high oil production. The fatty acid components of the above strains were analyzed by gas chromatography, and the fatty acid components were selected. The strai...
Embodiment 3
[0068] A method utilizing Schizochytrium to produce docosahexaenoic acid, comprising the following steps:
[0069] (1) Activating and culturing Schizochytrium to produce Schizochytrium seed liquid. Described Schizochytrium is obtained according to the following method:
[0070] The Schizochytrium collected in Example 2 is subjected to multiple ultraviolet-nitrosoguanidine compound mutagenesis, the mutagenesis method is, utilize 0.5mL PBS buffer solution (100mM, pH7.0) Then add 0.5mL of 2.5mg / mL nitrosoguanidine solution, and treat at 30 degrees Celsius for 1 hour; centrifuge the above treatment solution under sterile conditions, wash the bacteria with distilled water to make the bacteria liquid OD 650 After reaching 0.4 to 0.6, pour it into a petri dish to make the bacterial liquid into a thin layer of liquid with a thickness of 1mm to 5mm, and then irradiate it at a distance of 40mm for 30 seconds under a 25W ultraviolet lamp, and centrifuge the above-mentioned ultraviolet-t...
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