Method for producing docosahexaenoic acid by using schizochytrium limacinum

A technology of docosahexaenoic acid and Schizochytrium, which is applied in the field of fermentation, can solve the problems of increasing income and high energy consumption, and achieve the effects of increasing biomass, increasing production, and optimizing process parameters

Inactive Publication Date: 2014-06-25
WUHAN HUASHITE IND BIOTECH DEV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the current method of producing DHA using Schizochytrium is DHA, and the DHA content does not exceed 50%. Further increasing the DHA content will greatly improve product quality, thereby greatly increasing revenue without increasing costs.
At the same time, in order to maintain the normal growth of bacteria in the existing technology, it is necessary to maintain a large amount of ventilation in the fermenter, which consumes a lot of energy.

Method used

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  • Method for producing docosahexaenoic acid by using schizochytrium limacinum
  • Method for producing docosahexaenoic acid by using schizochytrium limacinum

Examples

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Embodiment 1

[0044] A method utilizing Schizochytrium to produce docosahexaenoic acid, comprising the following steps:

[0045] (1) Activate and cultivate Schizochytrium sp. S056 into Schizochytrium sp. seed liquid.

[0046] (2) Put the Schizochytrium seed solution into the fermenter according to the volume ratio of 2% for cultivation, the cultivation temperature is 28 degrees Celsius, ventilate and keep stirring at a constant speed, the ventilation volume is 10 cubic meters per hour to 50 cubic meters per hour, and at the same time, the flow is supplemented feed, the feed includes carbon source, nitrogen source and glycerin, and maintains the concentration of glycerol at 0 to 10 g / L. The carbon source is glucose, and the nitrogen source is yeast powder. Feed feeding is divided into three times, each feeding, when the carbon source concentration in the fermentation broth drops to 20.0 g / L to 30.0 g / L, start feeding, when the carbon source concentration in the fermentation broth is 30.0 g ...

Embodiment 2

[0056] A method utilizing Schizochytrium to produce docosahexaenoic acid, comprising the following steps:

[0057] (1) Activating and culturing Schizochytrium to produce Schizochytrium seed liquid. The Schizochytrium is obtained according to the following method: take a certain amount of water sample and apply it to a high-salt YPD solid medium (glucose 20.0g / L, yeast powder 10.0g / L, peptone 20.0g / L , agar 20.0g / L, sea crystal 10.0 g / L), cultured at 25.0 degrees Celsius for 48 hours, a large number of single colonies were obtained, picked single colonies in liquid medium, and cultured on a shaker at a speed of 180 rpm and a culture temperature of 25.0 degrees Celsius After 72 hours of shaking flask culture under certain conditions, the bacteria were stained and observed under a microscope to screen out the strains with high oil production. The fatty acid components of the above strains were analyzed by gas chromatography, and the fatty acid components were selected. The strai...

Embodiment 3

[0068] A method utilizing Schizochytrium to produce docosahexaenoic acid, comprising the following steps:

[0069] (1) Activating and culturing Schizochytrium to produce Schizochytrium seed liquid. Described Schizochytrium is obtained according to the following method:

[0070] The Schizochytrium collected in Example 2 is subjected to multiple ultraviolet-nitrosoguanidine compound mutagenesis, the mutagenesis method is, utilize 0.5mL PBS buffer solution (100mM, pH7.0) Then add 0.5mL of 2.5mg / mL nitrosoguanidine solution, and treat at 30 degrees Celsius for 1 hour; centrifuge the above treatment solution under sterile conditions, wash the bacteria with distilled water to make the bacteria liquid OD 650 After reaching 0.4 to 0.6, pour it into a petri dish to make the bacterial liquid into a thin layer of liquid with a thickness of 1mm to 5mm, and then irradiate it at a distance of 40mm for 30 seconds under a 25W ultraviolet lamp, and centrifuge the above-mentioned ultraviolet-t...

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Abstract

The invention discloses a method for producing a docosahexaenoic acid (DHA) by using schizochytrium limacinum. The method comprises the following steps of (1) activating and culturing schizochytrium limacinum into a schizochytrium limacinum seed solution; (2) introducing the schizochytrium limacinum seed solution into a fermentation tank for culturing under the culture condition of 25-28 DEG C, aerating and stirring at a constant rotation speed, meanwhile fluidly feeding materials including carbon sources, nitrogen sources and glycerin, and keeping the concentration of glycerin at 0-10.0 g / liter; (3) controlling the temperature of a fermentation stable period at 18-23 DEG C, aerating and stirring at a constant rotation speed; and (4) when the concentration of the carbon source in a fermentation liquor is less than 5.0 g / liter, completing fermentation and collecting wet cells to obtain docosahexaenoic acid-rich greases. After the method is applied to large-scale fermentation and production of docosahexaenoic acid, the biomass of schizochytrium limacinum and the DHA content can increase, the quality of a DHA grease product is greatly improved, the grease quality is further improved and the energy consumption and the production cost are reduced.

Description

technical field [0001] The invention belongs to the field of fermentation, and more specifically relates to a method for producing docosahexaenoic acid by using Schizochytrium fungus. Background technique [0002] Docosahexaenoic acid (DHA) is a polyunsaturated fatty acid with very important physiological functions. It is an important nutrient that the human body cannot synthesize in large quantities but is indispensable. DHA exists in a large amount in the nerve tissue of the human retina and cerebral cortex, which can promote the development of the brain and vision, improve intelligence, memory and vision; in addition, it can also prevent the deposition of cholesterol on the blood vessel wall, prevent or reduce atherosclerosis sclerosis and coronary heart disease. [0003] The traditional production route of DHA mainly comes from the extraction and purification of deep-sea fish oil. However, the resources of deep-sea fish oil are very limited, the production cost is high,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 余龙江朱圆敏陈伟周蓬蓬金文闻余金龙钱凯
Owner WUHAN HUASHITE IND BIOTECH DEV
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