179 results about "Schizochytrium limacinum" patented technology

The invention belongs to the field of microbial fermentation engineering, and discloses schizochytrium limacinum (Aurantiochytrium sp.SD116). The schizochytrium limacinum is stored in China Microbial Culture Collection Administration Committee General Microbial Center with a number of CGMCC No: 6208), and a method for fermenting and producing DHA (Docosahexaenoic Acid) grease utilizing high density of the schizochytrium limacinum. According to the schizochytrium limacinum and the method disclosed by the invention, a bacterial strain fermenting condition is optimized based on element supply and a fermenting control angle, a carbohydrate supplementation operation is carried out, therefore, high-density fermentation is achieved, dry cell weight finally reaches 70.43g / L, grease content reaches 50.1g / L, DHA occupies more than 35% of total content of fatty acid; moreover, bioactive substances such as beta-carotene, astaxanthin and squalene are contained. The complete set of technology is convenient to operate; higher biomass liveweight and DHA content can be obtained; fermenting cost can be reduced; and the method is suitable for industrial fermenting production.

The invention discloses a method for separating and purifying DHA (docosahexaenoic acid) and saturated fatty acid from schizochytrium limacinum oil. The method includes: firstly, saponifying, salting out and acidizing the schizochytrium limacinum oil under protection of nitrogen so as to obtain free mixed fatty acid, and separating the fatty acid different in degree of saturation by urea adduction fractionation so as to obtain filtrate and solids after filtration; concentrating and extracting the filtrate so as to obtain polyunsaturated fatty acid rich in DHA and DPA (docosapentenoic acid); and allowing the solids to leach by means of acidolysis, extracting the saturated fatty acid (mainly comprising palmic acid) and recovering urea, wherein the urea is recyclable. The method is performedat a low temperature, oxidization of the unsaturated fatty acid is avoided, biological activity and nutrition of the unsaturated fatty acid are kept intact, and the problem of residual solvent is avoided. Products are high in purity, and the obtained polyunsaturated fatty acid mainly comprises the DHA and the DPA and hardly comprises EPA (eicosapentaenoic acid), the content of the DHA and the DPAis higher than 93%, and the content of the palmic acid of the saturated fatty acid is higher than 82%.

The present disclosure provides a novel mutant strain of Schizochytrium limacinum having the Accession No. MTCC 5249, which produces lipids and extracellular polysaccharide (EPS) simultaneously. The disclosure further provides a process for simultaneous production of lipids and extracellular polysaccharide (EPS) from the novel mutant strain of Schizochytrium limacinum. The lipids produced from the novel mutant strain of Schizochytrium limacinum comprises docosahexaenoic acid (DHA). The disclosure also provides a food, feed, cosmetic, nutritional or therapeutic supplement for humans or animals comprising the cell biomass and extracellular polysaccharides (EPS) of the mutant strain of Schizochytrium limacinum. A cosmetic composition comprising the extracellular polysaccharides (EPS) of Schizochytrium limacinum is also provided that is useful as a base for cosmetics for topical application. The present disclosure further provides a pickle composition and a fat product having improved nutritive value.

The invention discloses a compound feed for Scylla serrata, comprising fish meal, shrimp head powder, soybean meal, rapeseed meal, fish oil, soybean phosphatide powder, brewers yeast, choline chloride, molt hormone, bread flour, calcium dihydrogen phosphate, sodium dehydroacetate, butyl hydroxy anisole, astaxanthin, Schizochytrium limacinum, Bacillus licheniformis, a composite feeding promoting agent, a complex vitamin additive, a composite mineral matter additive and a composite adhesive. The feed has balanced nutrition, is resistant to spoilage, has a long storage life, not only reduces feed cost but also promotes the growth and improves the color of the Scylla serrate. In addition, the feed is friendly to environment, has good palatability and feed promoting effect, has high utilization efficiency and good stability in water, and can enhance the immunity and survival rate of scylla serrata. The invention also provides a preparation method for the compound feed for scylla serrata, which is simple in technology, with the function substance in raw material undestroyed in the preparation process.

The present disclosure provides a novel mutant strain of Schizochytrium limacinum having the Accession No. MTCC 5249, which produces lipids and extracellular polysaccharide (EPS) simultaneously. The disclosure further provides a process for simultaneous production of lipids and extracellular polysaccharide (EPS) from the novel mutant strain of Schizochytrium limacinum. The lipids produced from the novel mutant strain of Schizochytrium limacinum comprises docosahexaenoic acid (DHA). The disclosure also provides a food, feed, cosmetic, nutritional or therapeutic supplement for humans or animals comprising the cell biomass and extracellular polysaccharides (EPS) of the mutant strain of Schizochytrium limacinum. A cosmetic composition comprising the extracellular polysaccharides (EPS) of Schizochytrium limacinum is also provided that is useful as a base for cosmetics for topical application. The present disclosure further provides a pickle composition and a fat product having improved nutritive value.

The invention relates to a schizochytrium limacinum strain for producing docosahexaenoic acid and belongs to the technical field of bioengineering. The strain is named as slug type schizochytrium limacinum JN168 and preserved at China Center for Type Culture Collection, and the preserving number is CCTCCNO: M2012074. By performing separation screening on water samples of the Yellow Sea water areaof Qingdao, Shandong Province, the schizochytrium limacinum strain which is used for producing docosahexaenoic acid (DHA) and is stable in characteristics is obtained, and high yield of docosahexaenoic acid can be achieved by liquid state or solid state fermentation at 25-30 DEG C.

The invention relates to an artificial particle feed for juvenile turbot. The artificial particle feed is characterized by being prepared from whitefish powder, euphausiid powder, scallop powder, beer yeast, starch, cod liver oil, yolk powder, whey powder, L-alanyl-L-glutamine, choline chloride, soybean lecithin, schizochytrium limacinum powder, haematococcus pluvialis powder, compound vitamins, compound mineral substances, Chinese herbal medicines and sodium alginate. As the various raw materials are added into the feed, the comprehensiveness of nutrition is guaranteed. An immunopotentiator is added into the feed, so that the non-specificity immunity of fish bodies is enhanced. The feed is green and pollution-free. The schizochytrium limacinum powder and the haematococcus pluvialis powder are added into the feed, and the content of vitamin E is improved, so that the whitening rate of the juvenile fish is effectively reduced. The Chinese herbal medicine components are added, so that the immunity of the juvenile fish can be improved and parasite diseases in a juvenile fish growing phase are prevented from happening.

The invention discloses an egg-laying poultry feed additive. The feed additive comprises microalgae powder of schizochytrium limacinum and also can comprise a microalgae powder composition of the schizochytrium limacinum and haematoculcus pluvialis. Furthermore, the invention discloses application of the egg-laying poultry feed additive to an egg-laying poultry feed. By adding the egg-laying poultry feed additive disclosed by the invention into a basal feed to prepare the egg-laying poultry feed for feeding poultry including chickens, ducks, gooses, quails, pigeons and the like, the contents of nutritional ingredients in poultry eggs are increased, especially the contents of the nutritional ingredients such as DHA (Docosahexaenoic Acid), EPA (Eicosapntemacnioc Acid) and astaxanthin in the eggs are increased, so the health-care and nutritional effects of the poultry eggs are greatly enhanced.

The invention discloses a method for producing a docosahexaenoic acid (DHA) by using schizochytrium limacinum. The method comprises the following steps of (1) activating and culturing schizochytrium limacinum into a schizochytrium limacinum seed solution; (2) introducing the schizochytrium limacinum seed solution into a fermentation tank for culturing under the culture condition of 25-28 DEG C, aerating and stirring at a constant rotation speed, meanwhile fluidly feeding materials including carbon sources, nitrogen sources and glycerin, and keeping the concentration of glycerin at 0-10.0 g / liter; (3) controlling the temperature of a fermentation stable period at 18-23 DEG C, aerating and stirring at a constant rotation speed; and (4) when the concentration of the carbon source in a fermentation liquor is less than 5.0 g / liter, completing fermentation and collecting wet cells to obtain docosahexaenoic acid-rich greases. After the method is applied to large-scale fermentation and production of docosahexaenoic acid, the biomass of schizochytrium limacinum and the DHA content can increase, the quality of a DHA grease product is greatly improved, the grease quality is further improved and the energy consumption and the production cost are reduced.

The invention belongs to the field of microbial fermentation engineering, and particularly relates to a technology of consolidated bioprocessing (consolidated bioprocessing, CBP). Cellulose is fermented and degraded by a cellulose degradation strain (such as clostridium thermal fiber) to generate saccharides and organic acids, such as glucose, lactic acid and acetic acid; and biodiesel and co-produced products with high additional values, such as timnodonic acid, docosahexenoic acid and protein feed, are produced by serial fermentation combined with large-scale heterotrophism or raising cultivation of microalgae (such as schizochytrium limacinum, chlorella and nannochloropsis oculata). The method has the characteristics that anaerobic cellulose degradation bacteria are utilized to degrade the preprocessed lignocellulose material, so that the cellulose and hemicelluloses are converted into carbon sources which can be utilized by the microalgae such as soluble saccharide and organic acids; the carbon sources are inoculated into a microalgae seed liquid to be fermented after a certain nitrogen source and other nutrient elements are added, and oil is accumulated to generate the biodiesel and other products with high additional values.

The invention relates to the technical field of pet foods and food processing thereof, in particular to pet food grains for clearing heat, reducing pathogenic fire and enhancing immunity and a preparation method of the pet food grains. The pet food grains for clearing heat, reducing pathogenic fire and enhancing immunity comprise the following components in parts by weight: 10-30 parts of chicken meat, 10-30 parts of fish meal, 10-30 parts of beef bone meal, 10-30 parts of cereals, 1-10 parts of dried egg yolk, 5-20 parts of deep sea fish oil, 5-20 parts of chicken oil, 1-10 parts of beet pulp, 0.1-5 parts of schizochytrium limacinum, 1-10 parts of fruits and vegetables, 1-10 parts of natural edible plants, 0.001-1 part of natural antioxidants, 0.1-5 parts of taurine, 0.1-5 parts of beer yeast, 0.1-5 parts of fructo-oligosaccharide, 0.1-5 parts of vitamins and 0.1-5 parts of trace elements. The pet food grain for clearing heat, reducing pathogenic fire and enhancing immunity has the characteristics of clearing heat, reducing pathogenic fire, strengthening the spleen and the stomach, promoting gastrointestinal motility, enhancing immunity and rapidly complementing energy.

The invention relates to marine microorganism cultivation technology, specifically to a medium used for culturing Schizochytrium limacinum and a production method for DHA by using the medium. The medium provided by the invention is a medium with a low chlorine ion concentration and has a chlorine ion concentration of 2 g / L, a potassium ion concentration of higher than 0.1 g / L and a sodium ion concentration of 1 to 20 g / L. With the medium provided by the invention, the output of DHA can basically maintain same or be higher, the degrees of corrosion of the medium on a series of production equipment like fermentation equipment, oil pressing equipment and extraction equipment are reduced, the service life of the equipment is prolonged, production safety is guaranteed, and the chlorine ion concentration in discharged sewage during production is reduced, thereby further reducing production cost.

The invention discloses schizochytrium limacinum named as Aurantiochytrium sp, and preserved in China General Microbiological Culture Collection Center (CGMCC) located at No. 3, courtyard 1, the western Beichen road, Chaoyang District of Beijing on Nov. 2nd, 2017, and the preservation number is CGMCC No:14849. The schizochytrium limacinum can be used for preparing DHA, docosapentaenoic acid greaseand squalene. The strain can resist high fermentation temperature, and the fermentation cost can be effectively lowered; the strain can rapidly grow under the condition that the temperature is 33-37DEG C, a large amount of polyunsaturated fatty acids (DHA and docosapentaenoic acid) and squalene grease can be accumulated, the growth speed is high, and the yield of polyunsaturated fatty acid grease is high.

The invention provides a mutant strain of Schizochytrium limacinum. The mutant strain of Schizochytrium limacinum is preserved in China Center for Type Culture Collection (CCTCC) on December 3rd, 2012, in Wuhan University, Wuhan, Hubei Province, China, with an accession number of CCTCC No. M2012494. In microbial oil produced by using a method which employs the mutant strain of Schizochytrium limacinum, the content of DHA is no less than 50 wt%. The oil contains a proper amount of beta-carotene, and since beta-carotene has antioxidation characteristics like removal of free radicals, so the stability of docosahexenoic acid oil can be enhanced.

The invention relates to the technical field of microorganisms, in particular to schizochytrium limacinum suitable for high-density culture and a method for producing grease rich in DHA. The schizochytrium limacinum is suitable for high-density culture and can be used for producing grease rich in DHA, the production method is low in cost, and the biomass and DHA yield are high. The classification naming of the schizochytrium limacinum suitable for high-density culture is (Schizochytrium sp.) SLTW3 and is preserved in the China Center for Type Culture Collection with the preservation serial number of CCTCC No: M2015591. According to the method for producing grease rich in DHA by means of the schizochytrium limacinum, the schizochytrium limacinum is used as the original strain, thalli are collected through high-density fermentation, and then grease rich in DHA is extracted. With the schizochytrium limacinum as the original strain, total biomass can reach 170-190 g / L after high-density fermentation culture, and the DHA yield can reach 25 g / L.

The invention relates to a high-yield DHA schizochytrium limacinum strain as well as a culture method and an application thereof. Schizochytrium limacinum (Schizochytrium sp.) BLCY is reserved in Common Microorganism Center of China Committee for Culture Collection of Microorganisms on July, 15, 2014, wherein the address is China Academy of Sciences Institute of Microbiology, #3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No:9461. The invention further relates to a culture method and an application of the strain. By optimizing the components of the culture medium and the culture conditions, the biomass of the schizochytrium limacinum after being fermented for 54-72 hours reaches 30-45 g / L, the DHA content reaches 8.6-12.5 g / L, which is higher than the fermentation yields of microorganisms reported in the existing patents and literatures.

The invention discloses a method for producing DHA (Docosahexaenoic Acid) by fermenting schizochytrium limacinum via a mixed carbon source. The method comprises the following steps: culturing a schizochytrium limacinum seed solution by utilizing a seed medium; inoculating the schizochytrium limacinum into a fermentation medium of a single glucose carbon source or the mixed carbon source of glucose and glycerol; and replenishing the glycerol or the mixed carbon source of the glucose and the glycerol when the surplus quantity of the glucose in the fermentation medium is 1% to 5% of the total weight of the original glucose. The method has the beneficial effects that not only are the advantages of rapid growth speed and high total lipid content of the schizochytrium limacinum fermented by the single glucose carbon source absorbed, but also the characteristic of high DHA content of the schizochytrium limacinum fermented by the single glycerol carbon source is realized, so that the utilization rate of substrates is increased; the fermentation level of the DHA is improved; relatively high algal oil quality is ensured at the same time. Thus, the method is very favorable for promoting the industrialization development of producing the DHA by fermenting the schizochytrium limacinum.

The invention relates to a method for promoting synthesis of DHA in schizochytrium limacinum grease, and particularly relates to a method for prompting synthesis of docosahexaenoic acid (DHA) in schizochytrium limacinum grease. The method is characterized in that the activity of malic enzyme in a tricarboxylic acid transport system is inhibited by adding an exogenous control factor, so that the content of the NADPH in the schizochytrium limacinum can be greatly reduced, the synthesis of a great amount of DHA can be promoted, and the exogenous control factor is sesamol. According to the method, a fermentable carbon source also can be selected to reduce metabolic flux of a pentose phosphate pathway in the bacteria, so that the content of NADPH in the schizochytrium limacinum can be greatly reduced, the synthesis of a great amount of DHA can be promoted, and the largest content of the DHA in the grease of final bacteria can reach 55.3 percent. The method is simple, easy, obvious in effect, capable of greatly increasing the content of DHA in the bacteria grease and is likely to realize the industrialized application.

The invention provides a process for producing DHA with lignocellulosic agricultural and forestry waste as a raw material in order to solve problems of industrial production of DHA in the prior art. The process includes the steps of pretreatment, saccharification, fermentation of schizochytrium limacinum, extraction of DHA and the like. The process adopts a combination of lignocellulosic biosaccharification and high-density schizochytrium limacinum fermentation; by adopting lignocellulosic biomass as the raw material, on the one hand, the production cost of DHA is significantly reduced; on theother hand, the problem of comprehensive utilization of the agricultural and forestry waste is also solved. In the process, a lignocellulose saccharification stage culture medium and a fermentation medium can be recycled so that water can be significantly saved, and the use of chemicals is reduced. In addition, the cellulose saccharification efficiency is 80-90%, the biomass of schizochytrium limacinum can reach 15-80g / L, and the production of DHA is 5-25g / L. Compared with the prior art, the process has the advantage that the production and yield of DHA are higher.

The invention relates to a method of supercritical extraction of unsaturated fatty acid of schizochytrium limacinum, which is characterized by comprising the following steps of: filling dried schizochytrium limacinum powder into an extraction kettle of a supercritical extraction device; setting technological parameters of the supercritical extraction device; then starting to extraction; simultaneously starting an ultrasonic strengthening device; generating ultrasonic wave with the electric power of 500W in each liter of the extraction kettle and closing the ultrasonic device after extraction for 9 to 11 minutes; and maintaining the technological parameters set in the step (2) to be constant, and continuously extracting for 45 to 55 minutes to obtain the unsaturated fatty acid extract. By preferring technological parameters for extraction and simultaneously applying strengthening action of the ultrasonic wave, the extraction method can improve extraction efficiency obviously under the premise that the property of extract is not changed; in addition, the method has short extraction time, and the extracted unsaturated fatty acid has high purity.

The invention provides a DHA fermenting high-yielding method. The DHA fermenting high-yielding method includes the steps that schizochytrium limacinum strains are domesticated through a culture medium with a strong oxidant, the domesticated strains are cultured through a fermenting culture medium, and fermenting high yielding of DHA can be achieved accordingly. After the domesticated strains are fermented, the DHA content is 57.9%, and oil-producing efficiency is 0.492 g / L / h; compared with a control group, the DHA content and the oil-producing efficiency are increased about 20% and 55% respectively. The domesticating method does not involve a genetic engineering method, does not harm the environment, does not increase manpower and material resources, is simple and convenient, and has economic benefits.

The invention relates to artificial pellet feed for adult turbot. The artificial pellet feed is characterized by comprising white fish meal, red fish meal, whey protein, soybean protein concentrate, soybean meal hydrolysate small peptide, scallop powder, chicken meal, high protein flour, alpha-starch, L-alanyl-L-glutamine, choline chloride, sea fish oil, soybean lecithin, krill meal, ocean red yeast, photosynthetic bacteria, Schizochytrium limacinum powder, haematococcus pluvialis powder, multivitamins, composite mineral substances, Chinese herbal medicines and sodium alginate. According to the feed, a plurality of kinds of raw materials are added into the feed, so that the comprehensiveness of nutrition is guaranteed. During actual culture of the turbot, the pellet feed for the adult turbot has the advantages that the feed is good in feeding promoting performance, fishes like the feed, are full easily and do not fall ill easily, the survival rate is high, and the feed coefficient is low; the digestibility and the feed conversion ratio are high; the growth rate of the fishes is high, and the culture cycle is short.

The invention discloses a formula feed for juvenile fish of takifugu rubripes and a preparation method of the formula feed. The feed contains the following components in parts by weight: 30-50 parts of whitefish meal, 10-20 parts of krill meal, 10-20 parts of earthworm powder, 1-10 parts of squid oil, 1-10 parts of hordein powder, 1-10 parts of rice bran, 2-12 parts of dried yellow wine lees, 5-15 parts of wheat germ oil, 1-5 parts of evening primrose seed oil, 0.1-0.5 part of soybean enzymolysis protein, 0.1-0.5 part of a mussel extract, 1-8 parts of schizochytrium limacinum powder, 2-6 parts of nannochloropsis oculata powder, 5-15 parts of functional additive components, 0.1-0.3 part of multivitamins and 0.1-0.5 part of multiminerals. The preparation method comprises the following steps: (1) manufacturing raw materials; (2) synthesizing a biological material; (3) converting to a cooked material; (4) puffing and granulating; (5) drying; (6) spraying and coating; and (7) performing vacuum drying. The feed disclosed by the invention can improve the survival rate of the juvenile fish of takifugu rubripes, improve the physique and the body color, promote the growth, reduce the enrichment of tetrodotoxin and reduce the cultivation cost.

The invention discloses a method for extracting intracellular grease by wall breaking of a schizochytrium limacinum fermentation broth, comprising the following steps: firstly pouring a DHA-rich schizochytrium limacinum fermentation broth into a wall-breaking pot; adjusting pH value of the fermentation broth to 6.0-8.0 with food-grade acid or alkali, controlling temperature to 35-60 DEG C, adding compound enzyme which accounts for 1.0-4.0% of the weight of the schizochytrium limacinum fermentation broth, keeping warm, stirring and carrying out enzymolysis for 3.0-5.0 h, and carrying out inactivation; carrying out sand milling on the fermentation broth obtained after enzymolysis directly by a sand mill so as to thoroughly break single cell walls / membranes in the fermentation broth; and separating a broken-wall liquid by a high-speed centrifuge to obtain an oil phase, namely the DHA-rich primary grease. The DHA-rich primary grease can be extracted effectively by wall breaking. Yield can reach more than 85%. The content of DHA is 30-63%. The method provided by the invention is economic, environmentally friendly and practical, and can be effectively applied in the industry.

The invention discloses a schizochytrium sp. genetic engineering strain overexpressing a squalene synthetase gene, as well as a construction method and application of the schizochytrium sp. genetic engineering strain. Schizochytrium sp. ATCC1381 is adopted as an original strain, an overexpression vector is constructed in escherichia coli through the means of genetic engineering, bleomycin is selected as a screening resistance gene, the highly conserved sequence 18S rRNA of eucaryon is used as a homologous arm, a linear overexpressed fragment is electro-transformed into the schizochytrium sp toobtain a genetic engineering strain with high yield of squalene, and a theoretical basis is laid for microbial produced squalene and product industrialization.

The invention discloses application of schizochytrium limacinum and preparation thereof in increasing of content of DHA in milk of ruminants. The preservation number of the schizochytrium limacinum atChina General Microbiological Culture Collection Center is CGMCC No. 13746. By schizochytrium limacinum powder prepared from the schizochytrium limacinum of the invention, the content of DHA in milkof the ruminants can be increased. The natural-source high-DHA-content milk is organic, safe and stable, is easy to absorb, and is safe and effective for people to take natural DHA, demands of consumers can be catered and met, and thus, the schizochytrium limacinum and the schizochytrium limacinum powder can be widely applied to the field of common food and the field of livestock breeding.

The invention discloses a feed capable of increasing the content of polyunsaturated fatty acid in beef. Schizochytrium limacinum powder, sunflower seed, apple pomace powder and bile acid are added to raw materials of a frequently-used feed for beef cattle, are combined and compounded to form a mixed diet, wherein the mass ratio of the schizochytrium limacinum powder to the sunflower seed, the apple pomace powder and the bile acid is 0.8-1.2:1-3:5-8:0.08-0.12, and the total weight of the schizochytrium limacinum powder, the sunflower seed, the apple pomace and the bile acid accounts for 8-12% of the total quantity of the feed. According to the feed disclosed by the invention, selected materials are excellent, economical and practical; after the beef cattle eat the feed which is weighed according to an appropriate compounding ratio, the content of unsaturated fat acid in fat tissues of meat products can be effectively increased, and particularly the content of CLA and the content of DHA are increased. Not only can the food flavor be improved, but also the cholesterol content in beef is reduced, so that the nutritional level of the meat products is greatly increased.

The present invention relates to a penaeus vannamei compound feed which includes the following raw materials in parts by mass: schizochytrium limacinum meal 15-20 parts, fish meal 20-25 parts, shrimp shell powder 2-4 parts, cuttlefish powder 2-4 parts, peanut meal 10-15 parts, soybean meal 15-20 parts, flour 15-20 parts, complex vitamins 0.3-0.4 part, choline chloride 0.3-0.4 part and zeolite powder 2-3 parts. The replacement of part of fish meal with the schizochytrium limacinum meal reduces the feed costs; The addition of the schizochytrium limacinum meal enables the amino acids of the feed to be more balanced and complementary, therefore improving the feed efficiency; the schizochytrium limacinum meal is rich in active substances which can enhance the immune function of the penaeus vannamei, prevent the occurrence of diseases, reduce drug use in shrimp farming, improve the survival rate and growth rate of the penaeus vannamei. The schizochytrium limacinum meal contains DHA, significantly reduces or completely doesn't require the addition of fish oil, reduces the excessive reliance on fish meal and fish oil, and the marine fish resources are protected, at the same time the feed cost is reduced and the shrimp farming benefit is improved.

The invention provides a schizochytrium limacinum strain as well as a mutagenesis method and an application thereof. In the prior art, although how to improve the content of DHA in a strain is reported, since the consideration factor is often single, how to significantly improve the content of DHA in the strain is not comprehensively explored which also is a key to achieve the industrialization of schizochytrium limacinum. Schizochytrium limacinum TC5-2 is obtained by virtue of an ion beam mutagenesis method, by combining a culture medium, optimizing a fermentation process and adding an exogenous factor clethodim, the content of DHA in the strain is significantly improved which is conductive to achieving the industrialization of production of DHA by virtue of schizochytrium limacinum.

The invention discloses a cane molasses pretreatment process and fermentation and production process. After a series of treatments of acidification, preliming, flocculation and the like are conducted on sugar industry by-product cane molasses, flocculate impurity is removed, and a molasses treating liquid conforming to DHA fermentation and production by schizochytrium limacinum can be obtained. Through the treatment, the cane sugar in the cane molasses is mainly resolved into reducing sugar, most colloid is removed, the removal rate of potassium ion is not less than 50%, the content of calcium ion is slightly reduced, and the fermentation and production requirements of schizochytrium limacinum are met. The pretreated cane molasses is utilized to be added into a schizochytrium limacinum culture medium according to the ratio of being 15-60% of that of the total reducing sugar to ferment and produce DHA. The added cane molasses treating liquid not only can provide part carbon source such as glucose and levulose for growth of schizochytrium limacinum, but also contains necessary vitamin and mineral element needed by growth and grease synthesis, is relatively low in price, and can be used as an excellent cheap raw material for DHA fermentation and production.