Method for extracting intracellular grease by wall breaking of schizochytrium limacinum fermentation broth

Abstract

The invention discloses a method for extracting intracellular grease by wall breaking of a schizochytrium limacinum fermentation broth, comprising the following steps: firstly pouring a DHA-rich schizochytrium limacinum fermentation broth into a wall-breaking pot; adjusting pH value of the fermentation broth to 6.0-8.0 with food-grade acid or alkali, controlling temperature to 35-60 DEG C, adding compound enzyme which accounts for 1.0-4.0% of the weight of the schizochytrium limacinum fermentation broth, keeping warm, stirring and carrying out enzymolysis for 3.0-5.0 h, and carrying out inactivation; carrying out sand milling on the fermentation broth obtained after enzymolysis directly by a sand mill so as to thoroughly break single cell walls / membranes in the fermentation broth; and separating a broken-wall liquid by a high-speed centrifuge to obtain an oil phase, namely the DHA-rich primary grease. The DHA-rich primary grease can be extracted effectively by wall breaking. Yield can reach more than 85%. The content of DHA is 30-63%. The method provided by the invention is economic, environmentally friendly and practical, and can be effectively applied in the industry.

Description

technical field [0001] The invention belongs to the field of combined mechanical wall breaking, and relates to a new wall breaking method for extracting primary algae oil (docosahexaenoic acid) rich in DHA from the fermentation broth of Schizochytrium fungus. technical background [0002] Schizochytrium, also known as Schizochytrium, belongs to a class of marine fungi belonging to Eumycota, Oomycetes, Saprolegniales, and Thraustochytriaceae. It is single-celled and spherical. Schizovibrio cells can accumulate a large amount of active substances useful to the human body, such as: the DHA content in its total lipids is very high, reaching 35% to 68%, and more than 90% of the fatty acids are neutral in the form of triglycerides. ester. Fatty acids with similar structures have low content and are easy to separate and purify. [0003] DHA is the abbreviation of docosahexaenoic acid (Docosahexaenoic Acid), commonly known as brain gold, which belongs to polyunsaturated fatty acid...

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Problems solved by technology

利用相关破壁技术从藻类和真菌生产DHA油脂已经逐步实现工业化生产,近几年虽出现诸如若干专利(如专利号94106621.5、00135338.1、200410082921.3、200410075426.X、200610125476.3、200610028869.2、200710025079.3、200810047859.2、200910033869.5、 200910111657.4, 200910159368.1, 200910225296.6, 201110077030.9, 201210491610.7, etc.) Involved in the traditional method of producing DHA unsaturated fatty acid from microalgae and Schizovibrio schizovibrio fermentation broth, and extracting DHA rich in DHA by combining enzyme method and mechanical method from Schizochytrium fermentation broth Primary oil process without precedent

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