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Method for producing DHA (Docosahexaenoic Acid) by fermenting schizochytrium limacinum via mixed carbon source

A technology of Schizochytrium and mixed carbon source, applied in the field of microbial application, can solve the problems of low efficiency, high production cost of algae oil DHA, high equipment investment and maintenance costs, etc., so as to improve the fermentation level and promote the development of industrialization. , the effect of improving substrate utilization

Inactive Publication Date: 2014-07-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional industrial production of algae oil DHA mainly uses glucose, but the production of 1 ton of DHA oil requires about 5 tons of glucose. The production cost of algal oil DHA is very high

Method used

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  • Method for producing DHA (Docosahexaenoic Acid) by fermenting schizochytrium limacinum via mixed carbon source
  • Method for producing DHA (Docosahexaenoic Acid) by fermenting schizochytrium limacinum via mixed carbon source
  • Method for producing DHA (Docosahexaenoic Acid) by fermenting schizochytrium limacinum via mixed carbon source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Utilize glucose or glycerol single carbon source fermentation to produce DHA

[0022] Schizochytrium seed medium: glucose 30g / L, yeast extract 5g / L, sodium glutamate 5g / L, Na 2 SO 4 15g / L, KH 2 PO 4 3g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O3g / L, K 2 SO 4 1g / L, VB 1 0.005g / L, VB 6 0.002g / L, VB 12 0.005g / L.

[0023] Schizochytrium fermentation medium: glucose (or glycerol) 100g / L, yeast extract 10g / L, sodium glutamate 15g / L, Na 2 SO 4 15g / L, KH 2 PO 4 3g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O3g / L, K 2 SO 4 1g / L, VB 1 0.008g / L, VB 6 0.002g / L, VB 12 0.008g / L.

[0024] The seeds were cultured for two generations, and each generation was carried out in a 50mL / 250mL Erlenmeyer flask with an inoculum volume of 8% (V / V), and cultured on a shaking table at 25°C and 200rpm for 48h. The second-generation seeds were placed into 50mL / 250mL Erlenmeyer flasks with an inoculum volume of 8% (V / V), and the fermentation conditions were 25°C and 200rpm.

[002...

Embodiment 2

[0029] Embodiment 2: Glucose and glycerol mixed carbon source feeding fermentation produces DHA

[0030] Schizochytrium seed culture medium is as described in Example 1.

[0031] In the Schizochytrium fermentation medium, the carbon source is 50g / L glucose and 50g / L glycerol, and the remaining composition is as described in Example 1.

[0032] Schizochytrium culture method as described in Example 1.

[0033]The results showed that the biomass was 42.12g / L with a 1:1 mixed carbon source fed at one time, the fermentation time was 5 days, that is, the substrate utilization rate was improved, the total fat accounted for 61.84% of the dry bacteria content, and the DHA accounted for the total The fatty acid content is 45.67%, and the resulting DHA yield is 11.90g / L, which is about 10% higher than that of single carbon source fermentation.

Embodiment 3

[0034] Embodiment 3: DHA is produced by glucose feed glycerol fed-feed fermentation

[0035] Schizochytrium seed culture medium is as described in Example 1.

[0036] In the Schizochytrium fermentation medium, the composition is as described in Example 1 except for the carbon source.

[0037] Schizochytrium culture method as described in Example 1.

[0038] In this example, 4 sets of fermentation experiments were carried out. The first two were to add glycerin when the residual sugar content was 50% to 70%, and the latter two were to add glycerin when the residual sugar content of glucose was 1% to 5%.

[0039] Experiment No. A used 50g / L glucose as the initial carbon source, and added 50g / L glycerin when the residual sugar content was 50%.

[0040] In experiment number B, 75g / L glucose was used as the initial carbon source, and 25g / L glycerol was added when the residual sugar content was 70%.

[0041] Experiment No. C used 25g / L glucose as the initial carbon source, and ad...

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Abstract

The invention discloses a method for producing DHA (Docosahexaenoic Acid) by fermenting schizochytrium limacinum via a mixed carbon source. The method comprises the following steps: culturing a schizochytrium limacinum seed solution by utilizing a seed medium; inoculating the schizochytrium limacinum into a fermentation medium of a single glucose carbon source or the mixed carbon source of glucose and glycerol; and replenishing the glycerol or the mixed carbon source of the glucose and the glycerol when the surplus quantity of the glucose in the fermentation medium is 1% to 5% of the total weight of the original glucose. The method has the beneficial effects that not only are the advantages of rapid growth speed and high total lipid content of the schizochytrium limacinum fermented by the single glucose carbon source absorbed, but also the characteristic of high DHA content of the schizochytrium limacinum fermented by the single glycerol carbon source is realized, so that the utilization rate of substrates is increased; the fermentation level of the DHA is improved; relatively high algal oil quality is ensured at the same time. Thus, the method is very favorable for promoting the industrialization development of producing the DHA by fermenting the schizochytrium limacinum.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and relates to a method for producing DHA by fermenting Schizochytrium with mixed carbon sources. Background technique [0002] Docosahexaenoic acid (22:6n-3) (DHA) is an important omega-3 series polyunsaturated fatty acids (omega-3 polyunsaturated fatty acids), which can promote infant intelligence and vision development, prevent cardiovascular Disease, anti-cancer, anti-inflammation, enhance immunity and other effects. Deep-sea fish oil is the traditional main source of omega-3 polyunsaturated fatty acids, but there are many problems, such as unstable quality, exogenous pollution, bad smell, high purification cost, etc. EPA and cholesterol contained in fish oil also make it unsuitable Added to maternity and infant food. On the contrary, microbial oils (single cell oils, SCO) are not only easy to achieve large-scale production, but also overcome the common problems of traditi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 常明李婧刘睿杰王兴国金青哲黄健花邹孝强王小三刘元法
Owner JIANGNAN UNIV
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