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Dot immunogold filtration assay kit for diagnosing cysticercosis cellulosae, preparation and application thereof

A porcine cysticercosis and immunofiltration technology, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of cumbersome operation, high detection cost, and slow speed, and achieve simple production process, improved detection efficiency, and easy operation Effect

Inactive Publication Date: 2006-11-22
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The object of the present invention is to provide a sensitive, specific, and efficient method for detecting human porcine cysticercosis in the existing gold standard immunoassay, which is slow in speed, cumbersome in operation, requires specific instruments or high in detection cost, etc. The result is reliable, the detection is convenient, and the efficiency is high, which can be used as a diagnostic kit for detecting porcine cysticercosis in livestock; and provides a simple, cost-saving, and time-saving preparation method of porcine cysticercosis antigen colloidal gold in the kit; and application The kit is a simple, rapid and accurate diagnosis method for porcine cysticercosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: (diagnosis porcine cysticercosis spotted gold standard immunofiltration kit)

[0043] The reaction box set in the box is a small plastic box with length 3cm×width 2.5cm×height 0.6cm, which is divided into two parts: the bottom of the box and the lid. Fill with water-absorbing material, place a 1.3×1.3cm nitrocellulose membrane between the bottom of the small round hole and the top of the water-absorbing material, close the lid to form a reaction box; the kit also contains colloidal gold containing cysticercosis antigen black reagent bottle, white reagent bottle with washing solution, glass capillary tube for spotting, porcine cysticercosis standard positive blood paper or serum, and 4 copies of negative blood paper or serum to test the effectiveness of the kit; The black reagent bottle and the white reagent bottle are composed of a flexible small-mouth plastic bottle body, a matching fine-hole drip nozzle and a bottle cap, and the capacity is 10ml or 20ml;...

Embodiment 2

[0047] Embodiment 2: (preparation method 1 of colloidal gold)

[0048] The present invention uses 1% chloroauric acid, 1% trisodium citrate, 1% tannic acid, 25mM K 2 CO 3Colloidal gold was prepared by tannic acid-sodium citrate reduction method with double distilled water as raw material. Sodium citrate is mainly a reducing agent, while tannic acid has the dual functions of reduction and protection, controlling the formation process of "crystal nuclei". This example A liquid, the proportioning ratio of each raw material component and content of B liquid is shown in Table 1:

[0049]

[0050] Heat the prepared liquid A and liquid B simultaneously in a water bath to 59-61°C, stir liquid A on an electromagnetic stirrer, quickly add liquid B, continue stirring for 1 minute, and heat to boiling within 10-13 minutes. Under the action of tannic acid-sodium citrate reducing agent, gold acid can reduce gold ions into gold atoms, and the color of the colloidal gold solution...

Embodiment 3

[0051] Embodiment 3: (preparation method 2 of colloidal gold)

[0052]

[0053] The preparation and synthesis methods of liquid A and liquid B are the same as in Example 1, and the colloidal gold particles produced have a diameter of about 12nm.

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Abstract

The provided colloid gold chromatography percolation reagent box for diagnosis of swine cysticercosis comprises: with cellulose nitrate as solid carrier, adsorbing swine sample blood; using antigen and colloid gold as the liquid phase, as well as the probe and indicator, forming visible red fleck. When using, dropping sample to the cellulose nitrate, adding 100 mul washing liquid and same antigen colloid gold, and forming the red fleck in 2-3min. This invention has strong specificity and well sensitivity, and more fit to practical application.

Description

technical field [0001] The invention relates to the technical field of diagnosis of livestock parasitic diseases, in particular to a preparation method and an application method of a dotted gold standard immunofiltration kit for diagnosing porcine cysticercosis. Background technique [0002] Cysticercosis, also known as cysticercosis cellulosae, is a zoonotic parasitic disease caused by the larvae of Taenia solium parasitizing humans or intermediate hosts such as pigs and wild boars. Human is not only the only final host of Taenia solium, but also a suitable host for its juvenile stage. [0003] Symptoms and hazards of the disease vary with different parasitic sites. Cysticerci parasitize in the brain and may present symptoms of encephalitis or epilepsy, dizziness, vomiting, sensory disturbance, paralysis, urinary retention, neurasthenia and other symptoms; parasitizing in the eyes can induce blindness; parasitizing in the internal organs can cause h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/532
Inventor 卢福庄张雪娟程天印项美华付媛方兰勇冯尚连程菊芬
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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