294results about How to "High fat content" patented technology

The invention discloses ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp, wherein the ankistrodesmus strain is SS-B7, the classification name of SS-B7 is Ankistrodesmus sp, and the ankistrodesmus sp SS-B7 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 15, 2013, and has the preservation number CGMCC No.7478. The ankistrodesmus sp SS-B7 has tolerance to CO2 and NOx with high concentrations, and has high carbon sequestration efficiency, the content of total lipids in the cells of the obtained biomass accounts for 40% or above of the dry cell weight, and the ankistrodesmus sp SS-B7 can be used for producing biodiesel.

Shredded or flaked whole grain-containing composite food products, such as ready-to-eat cereals, and sweet and savory snacks, are continuously produced by pelletizing cooked, tempered, whole cereal grain particles in the presence of vegetables, fruit, or dairy cheese. In another aspect, an enrobing coating containing chocolate is applied to a baked shredded laminate product of the pelletization, wherein the shredded product may further optionally include fruit added and present during pelletization.

The invention provides a novel photo-bioreactor system for culturing microalgae, and a method for culturing the microalgae by using the photo-bioreactor system. The method adopts different photo-bioreactors at different stages for culturing the microalgae, and the design of the photo-bioreactor of each stage reasonably takes the optimal culture condition of the microalgae at the stage into consideration so as to achieve the high-density culture and the product rapid accumulation of the microalgae, so a novel process and novel devices for the scale cultivation of the microalgae are provided.

The invention provides a method for inducing fresh water chlorella to fast accumulate grease through plant hormone jasmonic acid. The method is characterized by comprising the following steps of: (1) preparation of chlorella solution preparation: under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio is 12h/12h, culturing fresh water chlorella to logarithmic phase to obtain chlorella solution for conducting hormone induction, wherein the fresh water chlorella adopts NO.9 fresh water chlorella or NO.31 fresh water chlorella which both come from Freshwater Algae Culture Collection at the Institute of Hydrobiology, FACHB-collection; (2) acquisition of high-grease-content chlorella: adding jasmonic acid into 200ml of chlorella solution obtained in step (1) until the final concentration is 0.49-0.51mg/l; and (3) acquisition of high-grease-content chlorella: culturing the chlorella solution subjected to hormone induction in step (2) for 15 days under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio of 12h/12h, and centrifugally collecting the frond with high grease content. The method is simple and easy, low in cost, and the grease content of the target product is high, and can be used as a raw material of industrialized biological produced oil.

The invention provides freshwater chlorella sorokiniana XJ02 and application thereof in fixation of CO2 and production of microalgae oil. The preservation number of the chlorella is CCTCC (China Center For Type Culture Collection) NO: M2013104. The chlorella disclosed by the invention can be used for fixing 0.03-20% of CO2 at high efficiency, the CO2 fixation efficiency of chlorella is 130-343mg/L/d, the biomass (dry weight) concentration of the chlorella is 720-2250mg/L, the oil content of chlorella is 29.2-41%, the oil fatty acid of the chlorella is mainly composed of C16 and C18 short-chain fatty acids, and the chlorella is suitable for production of biodiesel. Compared with the reported microalgae, the chlorella has higher CO2 fixation efficiency and biomass yield; and the chlorella can be used for obtaining the microalgae oil at high yield by selecting an appropriate culture condition, thus the production cost of the microalgae oil can be greatly reduced. An excellent production algae is provided for high-efficient fixation of CO2 in the environment and preparation of biodiesel by the microalgae oil.

The invention relates to a micro-nano blocking lubricant for a drilling fluid and a preparation method thereof and belongs to the field of oil and gas field drilling and oilfield chemical auxiliary agents. The micro-nano blocking lubricant for the drilling fluid is prepared from the following components in parts by weight: 100-200 parts of a nano emulsion and 100 parts of composite graphite micropowder. The micro-nano blocking lubricant for the drilling fluid also contains a dispersant. The nanoemulsion is prepared from components including paraffin, white oil, a composite emulsifier, a co-emulsifier and water by a phase inversion emulsification method. The micro-nano blocking lubricant for the drilling fluid can resistant the temperature of 150 DEG C, does not have adverse effects to viscosity of the drilling fluid, can reduce the filter loss of the drilling fluid remarkably, improve the quality of mud cakes remarkably, reduce the frictional resistance of a drilling tool and improve the mechanical drilling speed, and can bridge and block micro-cracks of shale quickly by means of a small size effect to prevent solid particles and a filtrate from invading the stratum to achieve a good blocking effect, so that the micro-nano blocking lubricant for the drilling fluid is particularly suitable for blocking fine throats which are 0.05-50[mu]m.

The invention discloses Desmodesmus sp. rich in oil, and culture and application thereof. The Desmodesmus sp. is MH-04 preserved in China General Microbiological Culture Collection Center on April 24, 2015, with an accession number of CGMCC No. 10764. The Desmodesmus sp. can tolerate high-concentration CO2 and SO2, is capable of realizing photoautotrophic growth in virtue of exhaust gas or flue gas containing CO2 and SO2 to acquire biomass and has a high carbon sequestration rate; and the obtained biomass is rich in oil and can be used for production of biodiesel.

The invention relates to a method for preparing long-chain alkane through hydrolysis and in-situ hydrogenation and decarboxylation of micro-algal oil. The method comprises the following steps: 1) mixing the micro-algal oil and water, heating the mixture to generate a hydrolysis reaction, and treating to obtain C10-C22 fatty acid; 2) adding the C10-C22 fatty acid, a non-noble metal catalyst, a hydrogen donor and water into a high-temperature high-pressure reaction kettle together, and heating the mixture to 300 to 390 DEG C to perform a decarboxylation reaction for 1 to 6 hours, wherein the active ingredient of the non-noble metal catalyst is Cu-Ni, and a catalyst carrier is one of SiO2, ZrO2, Al2O3, MgO or MWCNTs; 3) cooling a reaction product, dissolving the reaction product with an organic solvent, and filtering to obtain a liquid phase product and a solid phase catalyst. According to the method, the long-chain alkane is prepared by catalyzing a micro-algal oil hydrolysis product to perform in-situ hydrogenation and decarboxylation by the non-noble metal catalyst in high-temperature water; compared with the prior art, the method has the advantages of simple process, zero hydrogen consumption and low catalyst cost; secondly, the total mass yield of the long-chain alkane in the method can reach not less than 75.6 percent.

The invention provides a fertilizing method for planting cigar tobacco. The fertilizing method comprises the following steps of: firstly, early fertilizing base fertilizer: in 20-30 days before transplanting, trenching on the formed land with the interval of 110cm, the depth of 10-15cm and the width of 8-10cm, uniformly paving the base fertilizer at the bottom of the trench and ridging; secondly, applying seedling protecting fertilizer for transplanting: when small cigar seedlings are transplanted, dissolving ammonium nitrate into water, injecting the mixed solution and pesticide as transplanting water into the root parts of the seedlings, wherein 3-5kg of ammonium nitrate is fertilized in each mu; thirdly, irrigating roots by using seedling restoring fertilizer: in about 7 days after the tobacco seedlings are transplanted, irrigating the roots by using 5-7g/mu of potassium nitrate and an insect infestation resisting pesticide and then covering a layer of fine soil around tobacco roots; fourthly, timely applying seedling eccentric fertilizing; fifthly, digging holes and applying and burying growth flourishing fertilizer and irrigating by matching the growth flourishing fertilizer with biogas slurry; and sixthly, carrying out additional fertilizer and spraying at the mature stage. The fertilizing method disclosed by the invention has the advantages of simplicity in operation, standard specification, strong pertinency and greatly-improved yield and quality of cigar tobacco.

The invention discloses a method for utilizing industrial wastes to produce microbial grease by means of fermentation and a special strain Rhodotorula glutinis Rh.g CGMCC No.2258. The method of the invention is a method for utilizing the industrial wastes (such as waste water of industrial production like starches, monosodium glutamate, paper making and so on, and/or fermentation industrial residues like soya residues and so on) as culture media to produce the microbial grease through fermentation of the Rhodotorula glutinis; the microbial grease obtained by the method also can be taken as material lipid to prepare biodiesel, and conversion rate can reach 80 to 99 percent. The production method of the microbial grease has the advantages of simple operation, low cost and high use value, and not only pollution of high concentration wastes which are discharged by fermentation industries like starches, monosodium glutamate, paper making, soya residues, etc. on the environment can be reduced but also the wastes can be converted into treasures by using the wastes to produce the grease which is a product in short supply in the current market, thereby the aim of resource utilization of the wastes is reached.

The invention provides a method for processing oilfield wastewater and fixing CO2 (carbon dioxide) by using microalgae; the method comprises the following steps of: using oilfield wastewater in which the crude oil content is lower than 10mg/L, the TOC (total organic carbon) is lower than 40mg/L and the COD (chemical oxygen demand) is lower than 100mg/L for microalgae cultivation and carbon fixing, introducing CO2 directly into the wastewater, selecting the microalgae with high stress resistance, high oil content and high function in degrading petroleum hydrocarbon according to the water quality of the oilfield wastewater introduced with the CO2, combining the large-scale cultivation of the microalgae with the wastewater treatment, building a large-scale cultivation system by using five stepped microalgae processing ponds which are connected with each other, and then adjusting the water temperature to be 20 DEG C to 35 DEG C; a silicon carbide micro-filtration membrane is used for harvesting, and 10-20% of the harvested microalgae is used as algae seed for the algae liquid cultivation of the next batch and 70-90% of the harvested microalgae is used for preparing biodiesel. The wastewater temperature which is the most proper for the growth of the algae is controlled by controlling the content of fresh wastewater, and the full-year stable and continuous running of the oilfield wastewater processing and CO2 fixing system is achieved.

The invention provides a water storage-type city road isolation belt having ecological purifying function. The water storage-type city road isolation belt comprises a U-shaped water permeable tank, curb bricks, discharge drains, discharge drain cover plates, an ecological purifying case and plants. The U-shaped water permeable tank is arranged below the center ground of the isolation belt, plants are planted in the U-shaped water permeable tank, the curb bricks are arranged at two sides of the upper surface of the U-shaped water permeable tank; the discharge drains are arranged at two sides of the U-shaped water permeable tank; the discharge drain cover plate is arranged at the top of the discharge drain; and ecological purifying case is arranged at one side of the bottom of the discharge drain close to the U-shaped water permeable tank. The apparatus has safety protection function as the common road isolation belts, can effectively degrade the pollutant carried by raining flow on the pavement, reduces the burden of downstream sewage processing, has water storage and soil moisture retention functions, prolonging the plant maintenance period for the road isolation belt, and saves the water used for irrigation. The road isolation belt has the advantages of simple manufacture, low cost, and convenient enforcement, and is suitable for all kinds of the city roads.

The invention relates to a culture method for improving golden algae biomass and grease content, and discloses a culture regulation and control method for improving the golden algae biomass and the grease content simultaneously by using a nitrogen source. The method comprises the following steps of: inoculating algae strains into the conventional f/2 sterilization culture medium at high density; adding a nitrogen source into a culture system every 12 to 72 hours; introducing CO2 for culturing at the concentration of 0.2 to 100mmol/L, the culture temperature of between 25 and 30 DEG C, the light intensity of 100 to 300mu mol.m<-2>s<-1> and the air introduction speed of 0.6 to 1.2vvm in the light-dark ratio of 14h to 10h; and harvesting within 8 to 10 days, wherein the highest density of algae cells can reach 1.3*10<8> cell/ml, the maximum dry weight can reach 3.12g/L, and the maximum grease content can reach 53 percent. By the method, high biomass is acquired, high grease content of the algae cells is realized, and the defects of nitrogen limitation on improvement of the grease content of the algae cells and reduction in the biomass at present are overcome.

The invention discloses a method for producing DHA (Docosahexaenoic Acid) by fermenting schizochytrium limacinum via a mixed carbon source. The method comprises the following steps: culturing a schizochytrium limacinum seed solution by utilizing a seed medium; inoculating the schizochytrium limacinum into a fermentation medium of a single glucose carbon source or the mixed carbon source of glucose and glycerol; and replenishing the glycerol or the mixed carbon source of the glucose and the glycerol when the surplus quantity of the glucose in the fermentation medium is 1% to 5% of the total weight of the original glucose. The method has the beneficial effects that not only are the advantages of rapid growth speed and high total lipid content of the schizochytrium limacinum fermented by the single glucose carbon source absorbed, but also the characteristic of high DHA content of the schizochytrium limacinum fermented by the single glycerol carbon source is realized, so that the utilization rate of substrates is increased; the fermentation level of the DHA is improved; relatively high algal oil quality is ensured at the same time. Thus, the method is very favorable for promoting the industrialization development of producing the DHA by fermenting the schizochytrium limacinum.

The invention discloses a cultivation method for promoting rapid accumulation of oil producing microalgae cells and grease. At first, under heterotrophic culture condition, the cells are stimulated to grow rapidly by utilizing fulvic acid; when the microalgae cells grow to the anaphase of logarithmic phase, cell concentration reaches 6.63g L<-1> at the moment; supernatant is removed through centrifugation so as to serve as a seed solution required in a subordinate phase. A 1000mgL<-1> fulvic acid mother solution is prepared by using pure water, and the fulvic acid mother solution is added in kuh1 culture mediums, so that the concentrations of the fulvic acidare 5, 25, 125, and 625mg L<-1> respectively. Then the obtained seed solution is added in the kuh1 culture mediums containing the fulvic acid of different concentrations, and the initial concentration of the seed solution is 2.3g L<-1>. Then the algae solution is subjected to continuous illumination cultivation, and sampling is performed every two other days for measuring biomass and grease content. The cultivation method disclosed by the invention is simple and feasible, and low in cost; the cultivation time is greatly shortened and the grease content is remarkably increased, and great significance is put forth for the industrialization of microalgae biodiesel production.

The invention discloses a method based on mixotroph microalgae oil accumulation, and belongs to the technical field of biodiesel. The method comprises the steps that a BG-11 basal culture medium adopting glucose as a carbon source is used for cultivating single algae, and algae cells are collected when the single algae grows to the rear logarithmic growth period; waste fermented liquid is dilutedto be adopted as the basal culture medium for single algae, and then, MgSO4 7H2O is added into the basal culture medium; the PH value is regulated to 6.8-7.1, and the culture medium is prepared; the prepared culture medium is subjected to high-pressure and high-temperature sterilization, oil-producing single algae is inoculated, the algae cell concentration is 0.9-1 g/L, then, absolute ethyl alcohol is added for dissolving a melatonin mother solution, the final concentration of melatonin in the culture medium is 10<-1>-10<-4> mol/L, and the algae solution is placed into strong light to be cultured; microalgae cultured to the stable stage is enriched, oil is extracted, and biodiesel is prepared. The method is simple and easy to implement, the production cost can be greatly lowered, the oilyield is remarkably increased, and therefore the biodiesel production efficiency of microalgae is greatly improved.

The invention discloses a purified feed used for constructing a nutritional obesity animal model as well as a preparation method and application of the purified feed. The purified feed is prepared from the following raw materials in parts by weight: 200-300 parts of casein, 3-5 parts of cysteine, 100-150 parts of maltodextrin, 50-100 parts of sucrose, 30-80 parts of cellulose, 20-50 parts of soya-bean oil, 250-360 parts of lard oil, 10-15 parts of mineral salt premix compound, 10-20 parts of calcium hydrogen phosphate, 5-10 parts of calcium carbonate, 15-30 parts of potassium citrate, 10-15 parts of vitamin premix compound, 2-4 parts of choline bitartrate, and 0.06-0.08 part of food-grade methylene blue stain. According to the purified feed, the purified raw materials with clear components are adopted, so that the oil content can be increased, the proportioning is reasonable, the quality is stable, the food with the form and the taste is suitable for being eaten, and the purified feed can be used for constructing a rodent nutritional obesity model, has an obvious effect and is good in repeatability.

The invention discloses a method for culturing marine oil-producing microalgae, which is characterized in that: the marine microalgae are culture by using daily sewage to supply nutritional salts and using high-concentration carbon dioxide to provide a carbon source, so the biomass yield of the marine microalgae is improved; and the microalgae are subjected to direct high-light exposure so as to improve the oil and fat yield of the marine microalgae. In the invention, the culture cost of the oil-enriched marine microalgae is lowered, the process of inducing the oil and fat accumulation in microalgae is simplified, and the industrialization of the large-scale culture of oil-enriched marine microalgae is promoted.

The invention relates to a microalgae cultivation method and a grease production method. The cultivation method comprises the following steps: a nitrogen fertilizer is provided for microalgae for many times; at least for one time, the nitrogen fertilizer is added when the nitrogen fertilizer in a microalgae suspension is consumed by microalgae until the microalgae is in a nitrogen-deficiency simulation state; and the cultivation time lasts for more than 10 days. By the cultivation method, normal growth of microalgae can be guaranteed, and the microalgae can accumulate much grease.

The invention discloses a method used for preparing grease via microalgae mixed cultivation. The method comprises following steps: 1, a microalgae culture medium and a Scenedesmus obliqnus FSH-Y2 seed solution are delivered into a photobioreactor, pH value of an obtained culture system is adjusted to be 10 to 12, CO2 content of added gas is controlled to be lower than 5v%, and culturing is carried out for 2 to 5 days; 2, pH value of the culture system is adjusted to be 8 to 10, an Ankistrodesmus sp. SS-B7 seed solution is added for mixed cultivation, and CO2 content of added gas is controlled to be 5 to 45v%; 3, culturing is carried out until stationary growth stage is over, and microalgae cells are harvested. The Scenedesmus obliqnus FSH-Y2 and the Ankistrodesmus sp. SS-B7 are stored in China General Microbiological Culture Collection Center on 11th September 2012 and 15th April 2013 respectively, and preservation number is CGMCC No. 6551 and CGMCC No.7478. The method is capable of increasing tolerance of the microalgae culture system on CO2, and solubleness, and increasing carbon sequestration efficiency; microalgae grease yield is increased obviously; and the method can be used for biodiesel production.