40 results about "Golden algae" patented technology

The invention provides an isochrysis galbana propagation method. The highest propagation density of isochrysis galbana is increased by adding an algal beneficial bacterium separated from isochrysis galbana, and the plateau maintenance time of isochrysis galbana is prolonged. According to the method, one specific strain of the used phycomycetes beneficial bacteria screened from isochrysis galbana is an Alteromonas sp. NBU-A-0001 strain, and the preservation number of the phycomycetes beneficial bacteria is CCTCC M 2020010; according to the method disclosed by the invention, the propagation speed of isochrysis galbana is much higher than the microalgae culture speed of a conventional microalgae nutrient solution; the highest propagation density and the plateau period maintaining time are farlonger than those of a conventional culture nutrient solution, more microalgae can be supplied under the same water body condition, and meanwhile, due to the fact that the plateau period is usually not prone to decay, microalgae quantity control and production management in the actual shellfish large-scale production process are quite facilitated.

The invention discloses a prawn nursery pond and a method for growing seedlings of the prawn which is applied to the prawn nursery pond. The prawn nursery pond is outdoor and open, the bottom of the prawn nursery pond is sandy , argillaceous or is laid with a black plastic film. The method for growing seedlings of the prawn comprises the steps of adopting the prawn nursery pond; inoculating golden algae and chlorella before a larva opens the mouth, and utilizing pure natural light to cultivate the algae; putting nauplius of the prawn into the nursery pond after the water quality if the nursery pond is stable; feeding rotifera in the phases of zoea and mysis after the nauplius opens the mouth; feeding the rotifera and moina Mongolica daday in post larvae phase; harvesting prawn seedlings when the post larvae grows to 4-6 periods. By utilizing the nursery pond and the method to cultivate the seedlings of the prawn, the growth of the larva is synchronized, the variable coefficient of the prawn seedlings is small, the specification is orderly, the survival rate is high, the cultivating speed is high, the cultivating cost is low, and the nursery pond and the method are beneficial to popularization in various regions.

The invention discloses a culture method of golden algae, which belongs to the water pollution control field. The invention takes a plant leaching liquor as an organic matter culture medium to culture the golden algae under the condition of room temperature and natural illumination or being put into an artificial climate box, and after the collection, the golden algae is used for controlling the water bloom. The phagocytosis experiment of water bloom algae indicates that the golden algae obtained by the method can be applied to the control of the water bloom. The culture medium used by the method of the invention for culturing the golden algae adopts the raw materials which are easy to be obtained, and have low cost, less energy consumption and high output, and no specific equipment is needed in the culture, thus being easy to be realized; and the cultured golden algae satisfies the requirement on application in the control of water bloom algae.

Disclosed are a preparing method and its product and application. Firstly, condense the pelagic unicellular alga in 1~2 degree, which is made into cream-condensed alga product, the dry it in low temperature or in vacuum, making the products which will not become harmful substance to juvenile of animal culture and can be diffused into alga juvenile of animal culture single cell with complete cell, finally, package it in vacuum or in nitrogen. The invention can be applied in all kinds of pelagic unicellular alga, and the products includes chlorella, nitzschia, chaetocero, can replace live pelagic unicellular alga to be applied in culture of seedling of animal culture in water, cultivation and strengthening of diet animal, especially for feeding diet animal .

The invention discloses a keep-alive concentration method for a chrysophyceae and a device for implementing the method. The invention has the advantages that through the adoption of the method and the device, the keep-alive ratio of the chrysophyceae reaches above 95% within 24 h after the chrysophyceae is concentrated to 30 to 100 million cells per liter. According to the keep-alive concentration method for the chrysophyceae, an external compression super-microfiltration membrane is adopted to concentrate to-be-concentrated chrysophyceae solution to obtain concentrated chrysophyceae solution, wherein the super-microfiltration membrane is a polyvinylidene fluoride super-microfiltration membrane with an aperture of less than 0.05 micron. The device for implementing the method comprises an injection pump, a chrysophyceae solution storage tank, a chrysophyceae solution pump, an external compression super-microfiltration membrane component, a back-flushing pump and a liquid waste storage tank, wherein the injection pump is communicated with the chrysophyceae solution storage tank by a first pipeline; the chrysophyceae solution storage tank is further communicated with the inlet of the external compression super-microfiltration membrane component by a second pipeline; and the liquid waste outlet of the external compression super-microfiltration membrane component is connected with a third pipeline inlet and a fourth pipeline outlet in parallel.

The invention relates to an algal composition in the form of an aqueous suspension. More particularly the invention relates to aqueous suspension composition including one or more algae selected from green algae, red algae, golden algae, brown algae, golden-brown algae, blue algae, blue-green algae or their species in the range of 0.1%-65% by weight with one or more surfactants in the range of 0.1%-50% by weight; with one or more structuring agent in the range of 0.01%-5% by weight, where the composition has a particle size range of from 0.1 micron to 60 microns. Furthermore, the invention relates to a process of preparing the algal composition comprising at least one alga and at least one agrochemically acceptable excipient in the form of an aqueous suspension. The invention further relates to a method of treating the plants, seeds, crops, plant propagation material, locus, parts thereof or the soil with the algal composition in the form of aqueous suspension.

The present invention discloses a method for culturing golden algea and an application thereof in controlling blooming algae, which belong to the field of water pollution control technology. The method for culturing golden algea is as follows: golden algea and microcystic aeruginosa are added into the BG11 culture medium; the golden algea lives on the microcystic aeruginosa to grow heterotrophically; after seven to ten days of culturing, the microcystic aeruginosa is gradually eaten up; the golden algea gets into the stable growing period; high-density golden algea liquid is obtained; and thegolden algea liquid is centrifugated in order to harvest the golden algea. The golden algea harvested by centrifugating or the golden algea liquid is directly added into the water body, and the golden algea swallows the blooming algae, so that the overgrowth of the blooming algae can be controlled and the blooming algae can be eliminated. The method which utilizes the golden algea to swallow the blooming algae to prevent the outbreak of the blooming algae and control the growth of the blooming algae is an algae-inhibiting method which is characterized by good ecological safety, cheap materials, economy, high efficiency and easy utilization, and has the advantages of easy golden algea culturing, good algae control effect, etc.

The invention relates to a golden algae (Poterioochromonas malhamensis), which is preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee with the preservation number CGMCC No. 11620. The present invention also relates to a method for cultivating Chrysophytes, which includes adding bran-free grains of grains and optionally nutrient salts and vitamins as nutrient sources for the growth of Chrysophytes into an aqueous system for cultivating Chrysophytes. The grain is selected from one or more of the following groups: rice, wheat, millet, black rice, buckwheat, oat, barley and sorghum, preferably rice and wheat. The nutrients and vitamins include NaNO 3 、KH 2 PO 4 、K 2 HPO 4 , Vitamin B 6 and vitamin B 12 . The isolated golden algal strain of the invention is easy to cultivate and grows rapidly. The cultivation method used in the invention is simple, the raw materials used for cultivation are cheap, and the cultivation cost is greatly reduced. At the same time, the method is established under the condition of bacteria and is very suitable for large-scale cultivation application.

The invention relates to the field of microalgae biotechnology, in particular to a method for concentrating and harvesting Isoflagellates by using ethanol and realizing semi-continuous culture, adding ethanol to the algae liquid of Isoflagellates waiting to be harvested after cultivation After standing still for 18-24 hours, the algae cells of Isochrysis globosa that settled at the bottom of the container were harvested by siphon method, and at the same time, new culture medium was added to the remaining algae liquid for re-inflated culture, so as to realize the semi- Continuous culture. Using extremely low concentration of ethanol to treat dinoflagellate algae to make them lose their ability to move, the majority of dinoflagellate cells in the algae liquid can be harvested by precipitation method, the recovery rate is as high as 70%, and the added Low ethanol concentration is very volatile, the harvested microalgae cells are safe and non-toxic, good resuspension performance, low cost of ethanol, sedimented algae cells siphon the residual dinoflagellate cells in the algae liquid after harvesting under aerated conditions Supplementing with new culture medium can still continue to proliferate, realizing the semi-continuous culture of Isoflagellates.

The invention provides a mixing fermentation method of chrysophyceae and photosynthetic bacteria, which belongs to the field of biotechnology. The invention concretely provides the mixing fermentation method of isochrysis galbana and ectothiorhodospira shaposhnikovii, isochrysis galbana and ectothiorhodospira shaposhnikovii are respectively subjected to seed activation, culture, and then mixing fermentation in an illuminating fermentation tank, so that isochrysis galbana content is greatly increased which is 5-10 times of that through normal culture.

The invention relates to a marine microalga and its culture method and application. The marine microalga is isochrysis sp. CCMM5001, is preserved in the china center for type culture collection (CCTCC), and has a preservation number of CCTCC NO:M2010138. The culture method comprises separating a single algae cell of isochrysis sp. CCMM5001, carrying out enlargement culture of the single algae cell under laboratory conditions, carrying out centrifugation in a fourth day after an isochrysis sp. CCMM5001 growth platform stage, and then drying to obtain isochrysis sp. CCMM5001 powder. The isochrysissp. CCMM5001 powder can be utilized as a biodiesel raw material. The culture method of the marine microalga utilizes carbon dioxide in industrial exhaust gas thereby reducing greenhouse gas discharge, and realizes absorption of oxynitrides in industrial exhaust gas thereby reducing environmental pollution.

The invention belongs to the technical field of algae, and discloses a method for reducing isochrysis-galbana adherence and increasing the growth amount. The method includes the following steps that an isochrysis-galbana seed solution is inoculated to a reaction pool containing a culture solution according to the inoculation amount of 5% to 10%, then 100 mg / L-200 mg / L amino acid is added, and themixture is cultured at the temperature of 20 DEG C to 22 DEG C, wherein the light-dark ratio is 12:12, and the ventilation rate is 0.4 vvm to 0.5 vvm; culturing is carried out for 48 h to 72 h, and algal cells are collected. By means of the method, the isochrysis-galbana adherence phenomenon can be obviously reduced, and the biomass of the algal cells is increased.

The invention provides a rapid detection of gold algae method and a test kit based on the cyclo-mediated isothermal expansion. Gold algae CoI gene is firstly provided as a molecular marker and applied in the rapid detection of gold algae based on the cyclo-mediated isothermal expansion, and a group of cyclo-mediated isothermal amplification primer is further provided, its specificity isothermally expands the molecular marker through the cyclo-mediate; and finally the rapid detection of gold algae method based on the cyclo-mediated isothermal expansion is provided, the method uses the to-be-tested sample granules and a gene group DNA as the template, and uses the specific primer group to conduct cyclo-mediated isothermal expansion reaction. The primer is strong in specificity, high in flexibility, and has very good repeatability. The method can detect the existence of gold algae mitochondria CoI genes with the lowest concentration of 100 copies / MuL and the existence of gold algae cells with the lowest concentration of 1000 cells / mL, and the sensitiveness is increased by 1000 times compared to the traditional PCR technique, the sensitiveness problem is very well solved, and the method has significant meaning for the early stage monitoring of microalgae culture contaminants.

The invention provides a bacterial strain of the genus Alteromonas and its application. The bacterial strain is screened from the algae liquid of Isoflagellates and can promote the growth of Isoflagellates. The strain of the genus Alteromonas provided by the present invention is named Alteromonas sp., the strain number is NBU-A-0001, the preservation number is CCTCC M 2020010, and the preservation date is January 3, 2020. The depository unit is China Center for Type Culture Collection, the address is Wuhan, Wuhan University. The Alteromonas bacterial strain obtained by screening in the present invention can promote the growth of the isochrysis globus, thereby effectively expanding the yield of the isochrysis globus and meeting the requirement of the isochrysis globus as feed.

The invention provides a method for preserving Isochrysis globosa, which belongs to the technical field of microalgae cultivation. The method comprises the following steps: 1) Cultivating Isochrysis globosa with an f / 2 medium containing a mixed antifreeze agent. Obtaining Isochrysis globosa culture medium in the logarithmic growth phase; 2) Adding ferric chloride to the Isochrysis globosa culture fluid described in step 1) for flocculation to obtain flocculated Isochrysis globosa cells; 3) The flocculated Isochrysis globosa cells are collected, freeze-dried, and sealed for storage; the mixed antifreeze agent in step 1) includes dimethyl sulfoxide and methanol. Using the method of the present invention to preserve Isochrysis globosa, after one year of storage at room temperature, the survival rate of the Isochrysis globosa cells can reach 92%, which is far greater than the cell survival rate of conventional preservation methods.

The embodiment of the invention discloses intelligentized illumination equipment and a spectra modulation method for golden alga culture. The intelligentized illumination equipment comprises a storage module, a judgment module, a duty ratio acquisition module and a light source module, wherein the storage module records the growth parameters of golden algae; the judgment module judges the current growth state of the golden algae; the duty ratio acquisition module acquires the growth characteristic parameters corresponding to the current growth state of the golden algae according to the current growth state of the golden algae and acquires blue-green light duty ratio corresponding to blue-green light currently needed by the golden algae and red light duty ratio corresponding to red light according to the growth characteristic parameters; and the light source module generates a corresponding blue-green light PWM (Pulse Width Modulation) signal according to the blue-green light duty ratio, generates the blue-green light with corresponding illumination intensity according to the blue-green light PWM signal, generates a corresponding red light PWM signal according to the red light duty ratio and generates the red light with corresponding illumination intensity according to the red light PWM signal. The intelligentized illumination equipment and the spectra modulation method which are provided by the embodiment of the invention can be used for increasing the light energy utilization efficiency of the golden algae on a light source, thereby increasing the growth speed of the golden algae.

The invention discloses a sinonovacula constricta sowing type culture method for experiments. The sinonovacula constricta sowing type culture method comprises the following steps: S1, removing impurities from bottom mud, disinfecting the bottom mud with quick lime, and exposing the bottom mud under the blazing sun; S2, placing the disinfected bottom mud in a breeding box, inserting a plurality ofbreeding pipes into the breeding box, wherein the height of the breeding pipes is larger than the thickness of the bottom mud; S3, adding the seawater disinfected by the bleaching powder into a culture box; S4, putting the axe foot end of the sinonovacula constricta fry facing downwards into the breeding pipes, and performing oxygenated culture; S5, regularly changing water, and feeding the mixedsolution of chaetoceros and chrysophyceae three times every morning, noon and evening. The method can effectively prevent local sinonovacula constricta from being too high in density, and the death rate is reduced. Meanwhile, the problem of mechanical damage during sampling can be effectively solved, and the damage rate is almost zero; the labor can be greatly reduced, and all bottom mud does notneed to be turned over blindly; the problem of diffusion of decayed bottom mud after the sinonovacula constricta dies can be effectively solved, the healthy breeding environment is guaranteed, and generation of waste bottom mud is reduced.

The invention belongs to the technical field of bioengineering, and particularly relates to a method for improving the culture density of isochrysis galbana. The method comprises the steps: activatinga marinobacter aquaeolei strain; culturing a marinobacter aquaeolei solution; carrying out sterile treatment on isochrysis galbana species; and producing an isochrysis galbana seed culture solution,wherein the density of isochrysis galbana is more than or equal to 10*10<5> / mL, and according to formal culture production of isochrysis galbana, the density of isochrysis galbana is 1.5*10<7> cell / mLor above. The isochrysis galbana and marinobacter aquaeolei co-culture system can generate a synergistic effect, so that the components of a culture medium can be fully utilized, and the isochrysis galbana and the marinobacter aquaeolei simultaneously benefit from each other and promote co-growth due to the physiological and ecological characteristics of the isochrysis galbana and the marinobacter aquaeolei. Under the condition of mixed culture of the isochrysis galbana and the marinobacter aquaeolei, the isochrysis galbana can continuously and quickly grow, the biomass higher than that of aconventional method is finally obtained, and effective technical support can be provided for related aquaculture baits and DHA-obtaining algae.