14 results about "Golden algae" patented technology

The invention belongs to the technical field of lake and reservoir water source risk prediction, and provides a lake and reservoir water source algae bloom risk early warning system based on a 16S / 18SrRNA gene expression quantity threshold, and the method comprises the following steps: S1, collecting a sample from a lake and reservoir water source surface layer water body; s2, using 7-gate water bloom algae specific primers for blue-green algae, green algae, diatom, euglena, dinoflagellate, chrysophyta and cryptoalga; s3, based on the qPCR standard curve, calculating the copy number of the 16SrRNA / 18SrRNA gene of the seven water bloom algae; s4, when the gene expression quantity of a certain algal bloom algae continuously reaches 106-7 copy number / mL for 7-12 days, determining that the algal bloom algae is in a window phase; according to the threshold value of the gene expression quantity of the water bloom algae 16SrRNA / 18SrRNA, the window period of water bloom algae cells can be accurately recognized, so that early warning of the algae bloom risk in the lake and reservoir water source is achieved, and early warning of algae bloom outbreak in the lake and reservoir water source is achieved one week or above in advance.

The invention belongs to the technical field of fishery breeding, and provides a low-oxygen-resistant low-fish-meal compound feed as well as a preparation method and application thereof. The low-fish-meal compound feed is prepared from the following raw materials: fish meal, soybean meal, soybean protein isolate, flour, chicken meal, cassava starch, fish oil, soybean oil, lecithin, monocalcium phosphate, compound vitamins, compound minerals, choline, vitamin C, DL-Met, Lys-HCL, Thr, astaxanthin and chrysophyta laminarin. Firstly, the soybean meal, the soybean protein isolate and other composite plant proteins and the chicken meal are adopted to replace traditional fish meal, amino acid composition is balanced, the nutrition proportion of the compound feed is optimized, and the proportion of the fish meal is reduced to 20%; in addition, compound antioxidants such as astaxanthin and chrysophyta laminarin are added into the compound feed disclosed by the invention, so that the oxidation resistance of fish bodies can be remarkably improved.

The invention discloses a method for producing chrysophyceae laminarin through high-density culture of chrysophyceae and an application of the chrysophyceae laminarin. The culture method comprises the following steps: (1) culturing a chrysophyceae species under low-temperature and low-light conditions, and then transferring the chrysophyceae species into a seed culture medium for culturing to obtain activated chrysophyceae species; (2) placing the activated algae species obtained in the step (1) under a dark condition for shake culture to obtain dark adaptation algae species; and (3) inoculating the dark adaptation algae seeds into a fermentation tank, and adding a basic culture medium and a fed-batch culture medium for fermentation culture. By selecting the nitrogen source and accurately controlling the ammonium concentration, the double improvement of the biomass and the yield of the chrysophyceae laminarin is realized, the adverse effect of the high-concentration nitrogen source on growth is avoided, and the culture efficiency and the product economy are improved.

The invention relates to the technical field of feed and feed additives. The invention provides an astaxanthin-rich cladophora sp. SYSU-A2024-1, the classification name of the cladophora sp. SYSU-A2024-1 is Oedacladium sp.SYSU-A2024-1, the cladophora sp. SYSU-A2024-1 is preserved in China Center for Type Culture Collection on June 3, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20241138. The invention also provides a composition containing the cladophora sp. SYSU-A2024-1 and the chrysophyta laminarin, and an application of the composition in preparation of a low-fish-meal feed for aquatic animals. The trachinotus ovatus low-fish-meal feed containing the composition prepared by the invention can more comprehensively improve the lipid utilization rate of trachinotus ovatus, relieve oxidative stress and inflammatory injury, improve the liver health of fish bodies and remarkably improve the growth performance of the fish bodies through synergistic interaction, thereby ensuring the health and vitality of the fish bodies.

The invention discloses a culture method for increasing the survival rate of young oysters, which comprises the following steps of: feeding 50 percent of chlorella, 20 percent of chrysophyceae, 15 percent of chaetoceros and 15 percent of platymonas in the early culture stage of the young oysters; the method comprises the following steps: feeding 30% of chlorella, 20% of chrysophyceae, 20% of chaetoceros and 30% of Platymonas subcordiformis at the later culture stage of the oyster seeds, managing the salinity, temperature and dissolved oxygen of an oyster seed culture water body, the culture density of the oyster seeds and the variety and matching proportion of biological baits, and improving the survival rate of the oyster seeds on the basis of original culture. The culture cost is reduced.

The present application relates to a kind of bait feeding mode suitable for northern oyster larva breeding work, at the D type larva period of northern oyster, feed mixed bait of chrysomonad and chlorella.The present application significantly improves the growth rate and survival rate of long oyster larvae by feeding mixed bait of chrysomonad and chlorella, and shortens the breeding cycle.

The invention relates to the technical field of aquatic breeding, and discloses an indoor cement pond artificial breeding method for babylonia lutosa, which comprises the following steps: sterilizing a water body with ultraviolet rays, storing oocysts of the babylonia lutosa, removing infected oocysts, and carrying out specific running water change 24 hours before hatching; after the larvae are hatched, a nutritional agent containing ethanol clostridium protein and agrocybe cylindracea polysaccharide is put every day, the larvae are fed with single-cell algae such as chrysophyceae, platymonas subcordiformis and chlorella in the early stage, and the larvae are fed with shrimp pond algae in the middle stage until the larvae are attached and During seedling culture, the seedling culture pond is continuously inflated, water dissolved oxygen, salinity, temperature, pH value and other parameters are controlled, and finally the snail seedlings are collected to end seedling culture. The artificial breeding method for babylonia lutosa in the indoor cement pool is suitable for one or more of babylonia lutosa and babylonia lutosa, and the success rate and the seedling quality of babylonia lutosa in the indoor cement pool can be effectively improved.

The invention provides a breeding method of coelomactra antiquata juvenile shellfish, and belongs to the technical field of shellfish breeding. In the breeding process of the coelomactra antiquata juvenile mollusk, the water temperature is kept to be 25-27 DEG C, the salinity is kept to be 26-28, and the coelomactra antiquata juvenile mollusk is fed with mixed live algae containing chlorella, chaetoceros mulleri, coelomactra zhanjiangensis and tetraselmis subcordiformis, so that the growth of the coelomactra antiquata juvenile mollusk can be promoted. According to the method, the composite probiotic liquid formed by combining bacillus subtilis, photosynthetic bacteria and lactic acid bacteria according to the specific proportion is sprayed every 5-10 days, accumulation of hydrogen sulfide and ammonia nitrogen in the substrate can be reduced, and water quality is purified. By means of the breeding method, the survival rate and the weight growth rate of the coelomactra antiquata juvenile mollusks can be effectively increased, the proportion of the coelomactra antiquata with the shell length larger than or equal to 4 cm can be effectively increased, and therefore the yield and quality of the coelomactra antiquata juvenile mollusks are improved.

The present application relates to the field of aquaculture breeding technology, and discloses an indoor cement pool artificial breeding method for east wind snail, which comprises the following steps: storing east wind snail egg sacs after disinfecting water body by ultraviolet rays, removing infected egg sacs, and performing specific water flow replacement 24 hours before hatching; feeding the larvae with a nutrient agent containing clostridium aceticum protein and agaricus blazei polysaccharide every day after hatching, feeding the larvae with monosomic algae such as chrysomonad, tetraselmis, chlorella, etc. in the early stage, and feeding the larvae with shelled algae in the middle stage until the larvae are attached and metamorphosed; keeping the breeding pool continuously aerated and controlling parameters such as dissolved oxygen, salinity, temperature and pH value of the water body during the breeding period, and finally collecting the snail seedlings to end the breeding. The indoor cement pool artificial breeding method for east wind snail provided by the present application is suitable for one or more of fasciolaria fasciolaris and mud east wind snail, and can effectively improve the success rate and seedling quality of indoor cement pool breeding of east wind snail.

The invention provides a strain of the genus laburensis. The provided laburensis can be used for remarkably improving the high-temperature resistance of isochrysis zhanjiangensis. The preservation number of the labrentz's bacteria is CCTCC (China Center for Type Culture Collection) NO: M2025963. The strain of the Labarenaria sp. Obtained by screening can effectively promote the growth of the isochrysis zhanjiangensis under high temperature stress, so that the biomass of the isochrysis zhanjiangensis is improved. Sufficient bait guarantee is provided for the shellfish planktonic larvae in the high-temperature season.

The invention provides a system for treating mariculture tail water through coupling of an algal-bacterial symbiotic system and a ceramic membrane, the system comprises a water inlet mechanism, a flow stabilizing mechanism, an MBGMBR component membrane pool and an aeration mechanism, an MBR membrane component is arranged in the MBGMBR component membrane pool, the aeration mechanism comprises a perforated aeration strip and an aeration pump, the perforated aeration strip is arranged at the bottom of the MBGMBR component membrane pool, and the aeration pump is arranged at the bottom of the MBGMBR component membrane pool. The aeration intensity of the aeration mechanism is 0.1 m < 3 > / m < 2 >. H-0. 5 m < 3 > / m < 2 >. H, bacteria and algae are arranged in the MBGMBR assembly membrane pool, the mass concentration ratio of the bacteria to the algae is (3-5): (1-2), the bacteria are activated sludge, and the algae are chrysophyta algae species, nitrogen, phosphorus and organic matters in the mariculture tail water are removed by utilizing different bacteria-algae proportions, so that the membrane pollution is effectively relieved, and the membrane pollution is reduced. Membrane blockage is prevented, and the service life of the membrane is prolonged.

The invention provides a beneficial algae bacterium and a method for culturing isochrysis galbana by using the beneficial algae bacterium, the beneficial algae bacterium separated from an isochrysis galbana solution is added to effectively improve the breeding density of the isochrysis galbana and inhibit harmful vibrio at the same time, the specific algae strain is isochrysis galbana 3018 (Isochrysis galbana) NMB-Ig-3018, and the preservation number is CCTCC (China Center For Type Culture Collection) 20241471. One specific bacterial strain is pseudoalteromonas aureus NMB-Ig-Pf-0001, and the preservation number of the pseudoalteromonas aureus NMB-Ig-Pf-0001 is CCTCC (China Center For Type Culture Collection) CM20241074. The invention further discloses a preparation method of the pseudoalteromonas aureus NMB-Ig-Pf-0001. According to the method disclosed by the invention, the breeding density of the isochrysis galbana is far higher than that of the isochrysis galbana cultured by a conventional Ningda No.3 culture medium, more microalgae can be supplied under the same water body condition, and the method is very beneficial to microalgae quantity control and production management in the actual large-scale shellfish production process. In addition, the effect of inhibiting vibrios is achieved while the density of the algae is increased, and poison of the vibrios in aquaculture water is reduced.

The invention relates to the technical field of algae culture, and discloses an isochrysis galbana heterotrophic culture method, which comprises the following steps: analyzing according to the combination of a monitoring time axis and a growth condition to obtain a growth abnormal value, if the growth abnormal value is greater than or equal to a growth abnormal threshold value, generating an analysis signal, obtaining pH culture data, and if the pH culture data is greater than or equal to the growth abnormal threshold value, analyzing the pH culture data; carrying out data processing on the suitable culture time ratio and the pH value exceeding area ratio to obtain a pH value deviation degree value, if the pH value deviation degree value is greater than a pH value deviation degree threshold value, marking the pH value deviation degree value as a pH value abnormal sub-cycle number, carrying out ratio processing on the pH value abnormal sub-cycle number and the growth abnormal sub-cycle number to obtain a pH value abnormal proportion, and calculating the growth abnormal sub-cycle number according to the pH value abnormal proportion. If the pH value abnormal proportion is larger than or equal to a pH value abnormal proportion threshold value, generating a suspected abnormal signal, obtaining deviation data, analyzing the deviation data to obtain a deviation correlation variance, and if the deviation correlation variance is smaller than a deviation correlation variance threshold value, indicating that the pH value is a reason influencing the growth condition, and adjusting according to the change of the pH value.

The invention relates to the field of algae preservation, in particular to application of supernate in chrysophyceae preservation, a chrysophyceae preservation method and application of the chrysophyceae preservation method. The invention discloses application of a chrysophyceae culture supernatant and an additive in prolonging the freezing shelf life of chrysophyceae, reducing clotting after freezing and thawing or reducing nutrient loss after freezing and thawing. The additive is glycerol and / or vitamin. The invention further discloses a method for preserving the chrysophyceae, the method comprises the step that the chrysophyceae preserving fluid is subjected to cryopreservation, the chrysophyceae preserving fluid comprises the chrysophyceae, the additive and the chrysophyceae culture supernatant, and the additive is glycerinum and / or vitamin. According to the cryopreservation method of the chrysophyceae, the preservation time of the chrysophyceae can be prolonged, after freeze thawing, the cell structure is complete, uniform in dispersion and free of obvious caking, nutritional ingredients are not prone to loss, and the cryopreservation method can be used for breeding spats and shrimp seeds.