A method for concentrating and harvesting Isochrysis globosa by using ethanol and realizing semi-continuous culture

A semi-continuous technology of Isoflagellates, applied in the field of microalgae biology, can solve the problems of cumbersome operation, high cost, flocculant residue and flocculant toxicity, and achieve the effect of simple collection, low volatility and easy operation

Active Publication Date: 2021-11-09
SHANGHAI OCEAN UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the problems of high cost, cumbersome operation (centrifugation method and membrane filtration method), and problems such as flocculant residue and flocculant toxicity (flocculant method) existing in the traditional dinoflagellate harvesting method , on the basis of repeated tests and screening of various substances, a method for harvesting Isoflagellates with ethanol and realizing semi-continuous culture is provided

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for concentrating and harvesting Isochrysis globosa by using ethanol and realizing semi-continuous culture
  • A method for concentrating and harvesting Isochrysis globosa by using ethanol and realizing semi-continuous culture
  • A method for concentrating and harvesting Isochrysis globosa by using ethanol and realizing semi-continuous culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Cultivate Isochrysis globosa with F / 2 culture medium until the relative growth decline period, to be harvested.

[0024] Among them, the F / 2 culture medium for cultivating Isochrysis globosa is: adding 74.8mg NaNO per liter of seawater 3 , 4.4mgNaH 2 PO 4 , 12mg Na 2 SiO 3 9H 2 O, 1mL trace element solution, 1mL vitamin solution. Trace element solution: 23mgZnSO 4 4H 2 O, 7.3 mg Na 2 MoO 4 2H 2 O, 17.8 mg MnCl 2 4H 2 O, 12 mg CoCl 2 ·6H 2 O, 10 mg CuSO 4 ·5H 2 O, 4.35 g Na 2 EDTA, 3.9 g FeC 6 h 5 o 7 ·5H 2 O, 1000mL distilled water. Vitamin solution: 0.5 mg vitamin B 12 , 0.5mg Biotin, 100mg Vitamin B 1 , 1000mL distilled water.

[0025] Among them, the inoculation density of Isochrysis globosa was 1×10 6 cell / mL, at a temperature of 25°C and a light intensity of 70μmol / m 2 s, photoperiod 16L: 6 days under the condition of 8D, algae cell density reaches 6.0×10 6 cell / mL, ready to harvest.

[0026] Take 50mL of the above-mentioned algae liquid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of microalgae biotechnology, in particular to a method for concentrating and harvesting Isoflagellates by using ethanol and realizing semi-continuous culture, adding ethanol to the algae liquid of Isoflagellates waiting to be harvested after cultivation After standing still for 18-24 hours, the algae cells of Isochrysis globosa that settled at the bottom of the container were harvested by siphon method, and at the same time, new culture medium was added to the remaining algae liquid for re-inflated culture, so as to realize the semi- Continuous culture. Using extremely low concentration of ethanol to treat dinoflagellate algae to make them lose their ability to move, the majority of dinoflagellate cells in the algae liquid can be harvested by precipitation method, the recovery rate is as high as 70%, and the added Low ethanol concentration is very volatile, the harvested microalgae cells are safe and non-toxic, good resuspension performance, low cost of ethanol, sedimented algae cells siphon the residual dinoflagellate cells in the algae liquid after harvesting under aerated conditions Supplementing with new culture medium can still continue to proliferate, realizing the semi-continuous culture of Isoflagellates.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a method for concentrating and harvesting Isoflagellates by using ethanol and realizing semi-continuous culture. Background technique [0002] Isochrysis galbana (Isochrysis galbana), Chrysophyceae, Isochrysis galbana. Single-cell motile individual, variable in shape, oval in shape, without cell wall, with two equal-length flagella. Because the individual is small, usually 4-7 microns long, 2.7-4.4 microns wide, 2.4-3.0 microns thick, no cell wall, easy to digest, rich in polysaccharides, carotene and high-energy lipids, it is aquaculture around the world (especially It is a widely used bait microalgae in shellfish farming). Isochrysis globosa is also a natural treasure house of physiologically active substances, among which β-carotene and highly unsaturated fatty acids such as EPA and DHA have the functions of anti-aging, anti-stress and promoting the development of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 黄旭雄薛毓才
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap