Strain of Alteromonas

A technology of alteromonas and strains, applied in the direction of unicellular algae, bacteria, isolated microorganisms, etc., can solve the problem of not considering the influence of interalgal bacteria, and achieve the effect of expanding production and promoting growth

Active Publication Date: 2020-07-24
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the actual microalgae propagation technology does not consider the influence of algae bacteria.

Method used

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  • Strain of Alteromonas
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Examples

Experimental program
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Effect test

Embodiment 1

[0013] Example 1: Screening of symbiotic strains

[0014] The strain of the present invention was isolated and screened from the tertiary culture medium of Isochrysis globosa in the aquaculture base of Fujian Baozhi Aquatic Products Co., Ltd. in June 2018.

[0015] The strain isolation method is as follows: Dilute the culture solution of Isoflagellates and spread it on 2216E solid medium. The culture condition is 28°C, protected from light, and cultivated upside down for about 1 week. Monoclonal colonies of different types, shapes and sizes can be obtained. . Single-clonal colonies with high degree of separation, clear borders, and diverse shapes were picked and transferred to a new 2216E solid medium, and purified strains were obtained by multiple bacterial three-section line methods. The purified strains were inoculated into 2216E liquid medium respectively, placed in a shaking incubator for cultivation, the cultivation condition was 28°C, protected from light, and the rota...

Embodiment 2

[0022] Embodiment 2: the application of Alteromonas bacterial strain in promoting the reproduction of Isochrysis globosa

[0023] First prepare the algae expansion culture solution, the composition is as follows: 100mg / L KNO 3 , 10mg / L KH 2 PO 4 , 3mg / L Fe-citrate 5H 2 O, 0.06g / L brown sugar, 6μg / L VB 1 , 0.05μg / L VB 12 .

[0024] In a 100mL glass Erlenmeyer flask, add 50mL of the above-mentioned sterilized new algae culture medium, add 100μL OD 600 =0.4~0.6 activated Alteromonas NBU-A-0001, insert 1mL algae cell density is 10 6 Cell / mL Isochrysis globosa species were mixed evenly, and cultured in a light incubator at 25°C, with a light intensity of 4000 Lux and a photoperiod of light / dark (12h / 12h).

[0025] In order to compare the effect of this multiplication method multiplication, in 100mL glass Erlenmeyer flask, use respectively the culture solution that has added Alteromonas NBU-A-0001 of the present invention, conventional microalgae culture solution (NMB3) and t...

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Abstract

The invention provides a strain of Alteromonas and application thereof. The strain is obtained from the algae liquid of Isochrysis sphaeroides and can promote the growth of Isochrysis sphaeroides. Thestrain of Alteromonas provided by the present invention is named Alteromonas (Alteromonas sp.), the strain number is NBU-A-0001, and the preservation number is CCTCC M20200010, and the preservation date is January 3, 2020. The preservation unit is the China Type Culture Collection, and the address is Wuhan University, Wuhan. The strain of Alteromonas screened and obtained in the present inventioncan promote the growth of Isochrysis globosum, thereby effectively expanding the output of Isochrysis globosum and meeting the requirement of Isochrysis globosus as a feed.

Description

technical field [0001] The invention belongs to the technical field of symbiotic bacteria screening, and in particular relates to a bacterial strain of the genus Alteromonas. Background technique [0002] Isochrysis galbana is small in size, rich in polysaccharides, carotene and other high-energy lipids, especially rich in unsaturated fatty acids DHA and EPA required for the growth and development of shellfish. It is an indispensable opening bait in the breeding process of seedlings. [0003] At present, domestic and foreign studies on the factors affecting the cultivation of Isoflagellates focus on the influence of environmental factors such as light, salinity, and temperature on the growth of Isoflagellates. The culture medium is organic and inorganic for the nutritional needs of microalgae. However, even if the suitable environmental and nutritional conditions are controlled as much as possible, the maximum reproduction density of microalgae is still not high enough in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12N1/12C12R1/01C12R1/89
CPCC12N1/02C12N1/12C12N1/20C12N1/205C12R2001/01
Inventor 徐继林孔周雁曹嘉懿周成旭严小军
Owner NINGBO UNIV
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