Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain of Alteromonas

A technology of alteromonas and bacterial strains, which is applied in the direction of single-celled algae, bacteria, microorganisms, etc., and can solve problems that do not consider the influence of algal bacteria

Active Publication Date: 2021-09-24
NINGBO UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the actual microalgae propagation technology does not consider the influence of algae bacteria.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Alteromonas
  • A strain of Alteromonas
  • A strain of Alteromonas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Screening of symbiotic strains

[0014] The strain of the present invention was isolated and screened from the tertiary culture medium of Isochrysis globosa in the aquaculture base of Fujian Baozhi Aquatic Products Co., Ltd. in June 2018.

[0015] The strain isolation method is as follows: Dilute the culture solution of Isoflagellates and spread it on 2216E solid medium. The culture condition is 28°C, protected from light, and cultivated upside down for about 1 week. Monoclonal colonies of different types, shapes and sizes can be obtained. . Single-clonal colonies with high degree of separation, clear borders, and diverse shapes were picked and transferred to a new 2216E solid medium, and purified strains were obtained by multiple bacterial three-section line methods. The purified strains were inoculated into 2216E liquid medium respectively, placed in a shaking incubator for cultivation, the cultivation condition was 28°C, protected from light, and the rota...

Embodiment 2

[0022] Embodiment 2: the application of Alteromonas bacterial strain in promoting the reproduction of Isochrysis globosa

[0023] First prepare the algae expansion culture solution, the composition is as follows: 100mg / L KNO 3 , 10mg / L KH 2 PO 4 , 3mg / L Fe-citrate 5H 2 O, 0.06g / L brown sugar, 6μg / L VB 1 , 0.05μg / L VB 12 .

[0024] In a 100mL glass Erlenmeyer flask, add 50mL of the above-mentioned sterilized new algae culture medium, add 100μL OD 600 =0.4~0.6 activated Alteromonas NBU-A-0001, insert 1mL algae cell density is 10 6 Cell / mL Isochrysis globosa species were mixed evenly, and cultured in a light incubator at 25°C, with a light intensity of 4000 Lux and a photoperiod of light / dark (12h / 12h).

[0025] In order to compare the effect of this multiplication method multiplication, in 100mL glass Erlenmeyer flask, use respectively the culture solution that has added Alteromonas NBU-A-0001 of the present invention, conventional microalgae culture solution (NMB3) and t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a bacterial strain of the genus Alteromonas and its application. The bacterial strain is screened from the algae liquid of Isoflagellates and can promote the growth of Isoflagellates. The strain of the genus Alteromonas provided by the present invention is named Alteromonas sp., the strain number is NBU-A-0001, the preservation number is CCTCC M 2020010, and the preservation date is January 3, 2020. The depository unit is China Center for Type Culture Collection, the address is Wuhan, Wuhan University. The Alteromonas bacterial strain obtained by screening in the present invention can promote the growth of the isochrysis globus, thereby effectively expanding the yield of the isochrysis globus and meeting the requirement of the isochrysis globus as feed.

Description

technical field [0001] The invention belongs to the technical field of symbiotic bacteria screening, and in particular relates to a bacterial strain of the genus Alteromonas. Background technique [0002] Isochrysis galbana is small in size, rich in polysaccharides, carotene and other high-energy lipids, especially rich in unsaturated fatty acids DHA and EPA required for the growth and development of shellfish. It is an indispensable opening bait in the breeding process of seedlings. [0003] At present, domestic and foreign studies on the factors affecting the cultivation of Isoflagellates focus on the influence of environmental factors such as light, salinity, and temperature on the growth of Isoflagellates. The culture medium is organic and inorganic for the nutritional needs of microalgae. However, even if the suitable environmental and nutritional conditions are controlled as much as possible, the maximum reproduction density of microalgae is still not high enough in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12N1/12C12R1/01C12R1/89
CPCC12N1/02C12N1/12C12N1/20C12N1/205C12R2001/01
Inventor 徐继林孔周雁曹嘉懿周成旭严小军
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com