37 results about "Brachymonas" patented technology

The invention belongs to the field of microbiological detection, and discloses a rapid detection chromogenic culture medium for pseudomonas, and the chromogenic detection culture medium comprises a chromogenic substrate, an endogenous enzyme promoter, a basic culture medium and a bacteriostatic agent. Specifically, the pseudomonas can react with a chromogenic substrate, so that a bacterial colony is blue-green, and other non-pseudomonas does not grow or weakly grow under the action of a bacteriostatic agent, or the chromogenic substrate different from the pseudomonas is utilized to display purple red or other colors which are inconsistent with the color of the pseudomonas bacteria, so that the pseudomonas bacteria can be distinguished, and the pseudomonas bacteria can be distinguished. And rapid separation and identification are realized. According to the rapid detection chromogenic culture medium for the pseudomonas, the glycoside chromogenic substrate and the endogenous enzyme accelerant are utilized, the problem that when the rapid detection chromogenic culture medium for the pseudomonas is prepared, the pseudomonas has chromatic aberration or does not have color development is solved, and the blank of the rapid detection chromogenic culture medium for the pseudomonas in the domestic market is filled.

The invention discloses a heterotrophic-aerobic denitrifying bacterium C-3 and application thereof, the heterotrophic-aerobic denitrifying bacterium C-3 is preserved in the China General Microbiological Culture Collection Center (CGMCC), is classified and named as brevundimonas, has the preservation number of CGMCC No.31729, and is preserved on August 23, 2024. The heterotrophic-aerobic denitrifying bacterium C-3 has the advantages that the preservation number is CGMCC No.31729, and the preservation date is 2024. The heterotrophic-aerobic denitrifying brevundimonas vesicularis C-3 (Brevundimonas vesicularis C-3) provided by the invention takes sodium acetate as a carbon source, and under the aerobic conditions that the C / N mass ratio is 10, the pH value is 7, the temperature is 30 DEG C and the rotating speed is 200r / min, a relatively good nitrate nitrogen removal effect is achieved, and the removal effect is 100%; in actual aquaculture tail water, nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and total nitrogen can be well removed; meanwhile, the strain has high safety and has good application value in treatment of aquaculture tail water.

The invention provides a primer combination for a multiple detection kit for marine pathogenic bacteria and the multiple detection kit. The primer combination comprises primer pairs and probes which are used for detecting 13 marine pathogenic bacteria: vibrio alginolyticus, vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio marine fish, vibrio river, vibrio menivibrio, vibrio mimicus, vibrio harveyi, vibrio occultus, vibrio horii, plesiomonas and aeromonas, and the sequence of the primer combination is as shown in SEQ1-SEQ39. According to the present invention, the genomic DNA of 13 marine pathogenic bacteria is identified and detected in one digital PCR reaction through the ultra-multiple digital PCR fluorescence coding technology, and the kit not only covers the common vibrio species such as vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus and the like, but also can identify the sea area vibrio, vibrio holii, vibrio occultus and the like, and the kit has good sensitivity, specificity and accuracy.

The invention discloses a component optimization method for preparing a liquid fertilizer from acetylisovaleryltylosin tartrate waste liquid, and relates to the technical field of fertilizer preparation, and the component optimization method comprises the following specific steps: step 1, selecting nanoscale activated carbon with the specific surface area of 1000m < 2 > / g-1500m < 2 > / g as a base material, the method has the advantages that a microbial flora system is constructed by screening out pseudomonas bacteria, actinomycetes and bacillus, and the pseudomonas bacteria can effectively decompose complex organic pollutants in the waste liquid by virtue of the mineralization effect of the pseudomonas bacteria and gradually convert the complex organic pollutants into carbon dioxide, water and simple organic acid, so that the primary purification of the waste liquid is realized; actinomycetes can utilize secreted enzymes to convert acetylisovaleryltylosin tartrate into small molecule compounds containing nitrogen, phosphorus, potassium and other nutrient elements, bacillus further promotes synthesis and stability of the nutrient small molecules, it is ensured that the liquid fertilizer is comprehensive in nutrient and accurate in content, the plant growth requirement can be better met, and the liquid fertilizer has a good application prospect. The resource utilization rate of the acetylisovaleryltylosin tartrate waste liquid is greatly improved.

This invention discloses a heterotrophic-aerobic denitrifying bacterium C-3 and its applications. The heterotrophic-aerobic denitrifying bacterium C-3 is deposited at the China General Microbiological Culture Collection Center (CGMCC), classified as *Brevundimonas naejangsanensis*, with accession number CGMCC No. 31729, and deposited on August 23, 2024. The heterotrophic-aerobic denitrifying vesicular *Brevundimonas naejangsanensis* strain C-3 provided by this invention exhibits excellent nitrate nitrogen removal efficiency (100%) under aerobic conditions using sodium acetate as a carbon source, a C / N mass ratio of 10, a pH of 7, a temperature of 30℃, and a rotation speed of 200 r / min. In actual aquaculture wastewater, it demonstrates excellent removal effects on nitrate nitrogen, nitrite nitrogen, ammonia nitrogen, and total nitrogen. Furthermore, this strain exhibits high safety and has significant application value in treating aquaculture wastewater.

To provide a citrulline-reducing agent for intraoral use.SOLUTION: The present invention provides a citrulline-reducing agent for intraoral use that contains vitamin B6. The citrulline-reducing agent for intraoral use inhibits citrulline production and / or secretion by intraoral bacteria such as those belonging to Porphyromonas, Fusobacterium, Prevotella, Veillonella, Treponema, or Streptococcus.SELECTED DRAWING: None

The invention belongs to the technical field of biomedicine, and particularly relates to a flora marker for predicting colorectal cancer liver metastasis and application of the flora marker. According to the present invention, the bacterial group marker is the combination of Acinetobacter, Streptococcus, Pseudomonas, Brevundimonas and Sphingomonas, and the bacterial group marker combination provides extremely high specificity (95.29%) and sensitivity (91.43%) when the early liver metastasis of colorectal cancer is predicted, such that the liver metastasis risk of the patient can be accurately assisted in the determination, and the early liver metastasis risk of the colorectal cancer can be accurately determined; the method has the advantages that reliable basis is provided for clinical decision making, the colorectal cancer liver metastasis can be predicted only by taking the abundance of the specific bacteria as an input index, the method is not limited by specific measurement means, and dependence on a detection technology is greatly reduced.

The invention relates to a Pseudomonas sp. strain for use in the production of a recombinant protein characterised in that said strain comprises a nucleotide sequence encoding a phi15 RNA polymerase. The invention further relates to a plasmid, capable of integrating or replicating in Pseudomonas sp., comprising a phi15 promoter sequence operably linked to a nucleotide comprising one or more restriction sites for the insertion of a nucleotide sequence encoding a recombinant protein, or operably linked to a nucleotide sequence encoding a recombinant protein.

The present disclosure relates to bacteriophages and compositions capable of infecting and killing Pseudomonas, and use of the same for treating Pseudomonas, e.g. Pseudomonas aeruginosa, bacterial infections.

A strain of Pseudomonas sp. bacterium, having been deposited with the Agricultural Research Culture Collection on 19 July 2023 with a deposit number of NRRL B-68295, exhibits fluorescene in the presence of perfluoroalkyl molecules. The strain may be cultured and lyophilized. A method for detecting perfluoroalkyls includes contacting the strain with a sample and observing whether fluorescence occurs.

The application provides a method for regenerating activated carbon by microorganisms. The method uses a regenerating bacteria solution composed of Beijerinckia sp. (LM-W, preservation number: CGMCC No. 17168) and / or Brachymonas sp. (LM-R, preservation number: CGMCC No. 17167) obtained by separation, purification and screening from the bottom sludge of paint spraying wastewater, and directly regenerates saturated or unsaturated activated carbon. Without any manual operation intervention and chemical reagent addition, the method can automatically decompose and desorb the organic matters in the saturated or unsaturated activated carbon, and further removes the inorganic matters and impurities in the pores of the activated carbon through ultrasonic high-frequency cleaning in a subsequent process, thereby further improving the regeneration rate of the activated carbon. The method for regenerating activated carbon by microorganisms has the advantages of simple system equipment, short time, low energy consumption, high economic benefit, environmental protection, no pollution, strong stability, large treatment capacity, no need to add any activating agent, high regeneration rate, and the like, and can overcome many defects of traditional methods for regenerating activated carbon by microorganisms.

The problem to be solved by the present invention is to provide an extract of bacteria of the genus Sphingomonas, which may be used as an inhibitor of the kallikrein-kinin system and can reduce the occurrence of redness.SOLUTION: The present invention relates to an extract of bacteria of the genus Sphingomonas, to a composition comprising at least one bacterium of the genus Sphingomonas or said extract, to the uses thereof and to the implementation thereof in a cosmetic method.SELECTED DRAWING: None

The invention provides a pseudomonas bacterium composition, a complex microbial inoculant and application of the pseudomonas bacterium composition and the complex microbial inoculant, and belongs to the technical field of microorganisms. The invention provides a Pseudomonas bacteria combination, which comprises Pseudomonas bacteria JF1 and Pseudomonas hutmensis JF2, the Pseudomonas bacteria JF1 and the Pseudomonas hutmensis JF2 are growth-promoting bacteria screened from rhizosphere soil of rhizoma corydalis, and the Pseudomonas bacteria JF1 and the Pseudomonas bacteria JF2 have the capabilities of dissolving phosphorus and producing siderophores, can be used as growth promoting agents in a synergistic manner to promote the growth of rhizoma corydalis seedlings, and can be used for promoting the growth of rhizoma corydalis seedlings. The method has a remarkable effect of promoting accumulation of the total alkaloid content in rhizoma corydalis tubers, can be used for artificial cultivation of rhizoma corydalis, provides a theoretical basis for improving the yield and quality of rhizoma corydalis, and has important guiding significance for reasonably and effectively utilizing microorganisms to improve the quality and yield of Qinshi medicine-rhizoma corydalis.

The invention discloses an intestinal microbial marker combination and system for detecting fish liver injury and application, and belongs to the technical field of molecular detection of fish liver injury. The invention relates to an enteric microbial marker combination, which is characterized in that the enteric microbial marker combination comprises the following components: cetyl, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella. By utilizing the intestinal microbial marker combination, non-invasive diagnosis of liver injury can be realized on the premise of not invading a fish body, the accuracy is high, missing detection is avoided, and particularly, the condition that liver injury is misjudged to cause excessive treatment is avoided. A judgment basis is provided for breeding environment regulation and control and precise disease prevention and treatment, the breeding survival rate of the pelteobagrus fulvidraco is increased, and the breeding benefit is improved.

Customized recombinant proteins are designed and produced by cultures of Pseudomonas bacteria, including natural and recombinant silk proteins, fluorescent proteins, and elastin-like proteins (ELPs). The recombinant genes can be expressed via insertion directly into the Pseudomonas bacteria, or via the transformation of a suitably designed recombinant plasmid. Advantageously, the carbon source used as the nutrient source by the Pseudomonas bacteria is derived from non-traditional nutrient sources, such as exogenous rhamnolipids, hydrocarbons, polyolefins, polyesters, and pyrolysis products of waste plastic, e.g., pyrolysis products of polyethylene or poly (ethylene terephthalate). The waste feedstocks can be added to particularly designed growth media for sustained bacterial culture and protein production. These feedstocks allow for upcycling of plastic waste into high value protein products, such as recombinant silk fibroins.

This invention relates to the field of microbial technology, and more particularly to a broad-spectrum Aeromonas hydrophila phage and its applications. The Aeromonas hydrophila phage AhP2505006 is classified as follows: Aeromonas hydrophila Phage AhP2505006, with accession number CCTCC NO: M20251307, exhibits cross-species lytic activity. It can effectively lyse *Aeromonas*, *Vibrio parahaemolyticus* and *Vibrio alginolyticus*, and *Citrobacter freundii*. It also possesses excellent targeting ability against multidrug-resistant strains and broad host spectrum characteristics, achieving a lysis rate of up to 80% against multidrug-resistant *Aeromonas hydrophila*. In the prevention and control of diseases caused by various pathogens, or in practical scenarios facing the limitations of traditional antibiotics and highly specific bacteriophages, it has become a highly promising solution.

Disclosed herein are compositions comprising A) compound of Formula One (F1) or Formula Two (F2); and B) biological agent selected from the group consisting of Bacillus species, Baclovirus species, Pseudomonas species, Beauveria species, Mycobacterium species, Mycorrhizal fungi, Trichoderma species, and Metarhizium species.

This invention belongs to the fields of bioinformatics and medical testing technology, specifically relating to a combination of biomarkers related to the postoperative prognosis of colorectal cancer and their application and prediction system. This invention, through 16S rRNA gene sequencing of tumor mucosal samples from colorectal cancer patients, combined with long-term postoperative follow-up data and screening, found that *Peptostreptococcus*, *Fusobacterium*, *Bacteroides*, *Porphyromonas*, and *Prevotella* are closely related to postoperative recurrence of colorectal cancer, with an AUC of 0.81 for assessing postoperative recurrence. Based on this, this invention conducts non-targeted metabolomics analysis, finding that combining alanine glutamate, putrescine, arginine, histidine, and sebacic acid with the above microbial biomarkers can improve the AUC to 0.91, significantly enhancing accuracy, specificity, and precision. This enables prognostic stratification of recurrence-free survival in colorectal cancer patients, demonstrating higher predictive efficacy and stability.

This invention provides a Pseudomonas spp. bacterial composition, a compound bacterial agent, and their applications, belonging to the field of microbial technology. This invention provides a Pseudomonas spp. bacterial composition, comprising Pseudomonas spp. bacteria (… Pseudomonas arcuscaelestis JF1 and ( Pseudomonas hutmensis JF2 and JF2 are growth-promoting bacteria screened from the rhizosphere soil of Corydalis yanhusuo. Pseudomonas bacteria JF1 and JF2 have the ability to solubilize phosphorus and produce iron carriers. As growth promoters, they can be used synergistically to promote the growth of Corydalis yanhusuo seedlings. In particular, they have a significant effect on promoting the accumulation of total alkaloids in Corydalis yanhusuo tubers. They can be used in the artificial cultivation of Corydalis yanhusuo, providing a theoretical basis for improving the yield and quality of Corydalis yanhusuo. They have important guiding significance for the rational and effective use of microorganisms to improve the quality and yield of "Qin medicine"—Corydalis yanhusuo.

The invention provides a pseudomonas bacterium, a microbial inoculum and application of the pseudomonas bacterium and the microbial inoculum, and belongs to the technical field of microorganisms. The invention provides a strain of Pseudomonas aagonensis JF3, and the preservation number of the Pseudomonas aagonensis JF3 is CGMCC (China General Microbiological Culture Collection Center) No. 34848. In the invention, the pseudomonas bacterium JF3 is a growth-promoting bacterium screened from rhizosphere soil of rhizoma corydalis, has the capabilities of fixing nitrogen, dissolving phosphorus and producing siderophores, can be used as a growth promoting agent to promote the growth of overground parts of rhizoma corydalis seedlings, and particularly has a remarkable effect of promoting accumulation of total alkaloid content in rhizoma corydalis tubers; the method can be used for artificial cultivation of the corydalis tuber, provides a theoretical basis for improving the yield and quality of the corydalis tuber, and has important guiding significance for reasonably and effectively utilizing microorganisms to improve the quality and yield of Qinshi medicine-corydalis tuber.

The present disclosure relates to compositions of bacteriophages, and use of the same for medical and non-medical applications, in particular treatment of Pseudomonas infection.

The invention belongs to the technical field of environmental microbiology, is applied to biodegradation of residual 2-methyl-4-chlorophenoxyacetic acid in the environment, and particularly relates to a 2-methyl-4-chlorophenoxyacetic acid degrading strain and application thereof. The Sphingobium sp. S-6 separated from soil belongs to sphingomonas and is preserved in the China Center for Type Culture Collection, the preservation date is April 2, 2025, the preservation number is CCTCC NO: M2025674, and when the temperature is 16-30 DEG C and the pH value is 4-8, the Sphingobium sp. S-6 has a good degradation effect on chipton and has high environmental adaptability, and the Sphingobium sp. S-6 can be used for degrading chipton. An efficient degradation strain resource is provided for elimination and control of residual 2-methyl-4-chlorophenoxyacetic acid in the environment, and important theoretical and application values are achieved.

The invention relates to the technical field of biological detection, and particularly discloses a multiple PCR (Polymerase Chain Reaction) detection primer group, a kit and a detection method for mastitis pathogenic bacteria. The invention discloses a multiple PCR (Polymerase Chain Reaction) detection primer group for mastitis pathogenic bacteria, which comprises the following primer pairs: a primer for amplifying a staphylococcus aureus gene, a primer pair for amplifying a streptococcus agalactiae gene, a primer pair for amplifying a pseudomonas gene and a primer pair for amplifying a klebsiella pneumoniae gene, the sequences of the primer pair for amplifying the escherichia coli gene are respectively shown as SEQ ID NO.1-10. The invention also discloses a kit for amplifying the escherichia coli gene. According to the application, a method capable of rapidly and accurately detecting and identifying streptococcus agalactiae, staphylococcus aureus, escherichia coli, pseudomonas and klebsiella pneumoniae with high sensitivity is established by utilizing a multiplex PCR technology.

The present invention provides for a method to increase production of isoprenol by a genetically modified Pseudomonas cell, the method comprising: (a) providing a genetically modified Pseudomonas cell comprising one or more of heterologous genes encoding: MvaE, AtoB, MvaS, MK, PMDHKQ, AphA, and PhoA; and (b) culturing or growing the genetically modified Pseudomonas cell in a medium to produce isoprenol; wherein (i) the genetically modified Pseudomonas cell is deleted, knocked out, or reduced in expression of one or more of the following endogenous genes: a gene at PP_2675 locus (or a deletion of the PP_2675 locus), phaABC, mvaB, hbdH, ldhA, gntZ, ppsA, pycAB, gltA, and aceA, and / or (ii) the medium comprises one or more amino acids that reduce the catabolism of isoprenol.

The invention relates to the technical field of microorganisms, and discloses a strain of minimum micromonas 324B6 and application thereof. The pseudomonas sp. 324B6 is preserved in the China General Microbiological Culture Collection Center on November 8, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 28905. The bacterial strain 324B6 of the invention belongs to a new species in the genus Pseudomonas, can tolerate high-concentration cyanogens, realizes efficient ammoniation and removal of cyanogens, and also can effectively degrade a variety of organic pollutants, including methanol, ethanol, THF, dichloromethane, DMF and triethylamine, at the same time, the bacterial strain can effectively degrade a variety of organic pollutants, including methanol, ethanol, THF, dichloromethane, DMF and triethylamine.

The present invention relates to a brown rice fermented product and use thereof, and in particular, to use of a brown rice fermented product in facilitating bowel relaxing and intestinal flora regulation. It has been discovered that the brown rice fermented product can relieve constipation, has an NO scavenging effect, and can down-regulate the relative expression levels of nos1, nos2a, and nos2b genes; intervention with the brown rice fermented product can restore the species richness of intestinal flora in constipated zebrafish, and has a certain influence on the species evenness and diversity, thereby increasing the abundance of beneficial bacteria (Bacteroidetes) while reducing the abundance of harmful bacteria (Firmicutes, Aeromonas, and Enterobacteriales). The brown rice fermented product of the present invention can be widely used as a functional raw material in the field of food products.

The invention belongs to the field of microbial identification, and discloses a biochemical value identification method of pseudomonas. Eleven biochemical reaction experiments such as glucose assimilation and maltose assimilation are performed by using known pseudomonas standard strains and isolates to obtain positive probability matrixes of different types of pseudomonas in different biochemical reaction experiments. The reaction positive rate data is obtained through statistics after a large number of isolates are subjected to biochemical experiments, and is accurate and high in effectiveness. And calculating mode frequencies, total occurrence frequencies, multi-item total occurrence frequencies, identification percentages and mode likelihood ratios T of different known and to-be-identified pseudomonas based on the probability matrix to realize identification of strains. According to the biochemical value identification method disclosed by the invention, negative or positive identification results presented by the related biochemical reaction are clear in boundary and easy to distinguish, identification and separation of seven common pathogenic bacteria species and three common environmental strains of the pseudomonas can be realized, the identification accuracy rate reaches 95% or above, and the identification results are stable.

A method of producing an alcohol or alcohol precursor from a cellulosic material and a genetically modified live ethanologenic organism, wherein the method comprises the steps of:exposing said genetically modified live ethanologenic organism to a culture media with a pH of between 2 and 9 thereby creating an incubation mixture;exposing said incubation mixture to a source of cellulose;incubating said genetically modified live ethanologenic organism in said incubation mixture with said source of cellulose under aerobic and / or anaerobic conditions at a temperature ranging from 0° C. to 60° C. for a first period of time sufficient for the degradation of cellulose, thereby creating a degraded cellulose mixture;optionally, incubating said genetically modified live ethanologenic organism in said degraded cellulose mixture under aerobic and / or anaerobic conditions at a temperature ranging from 0° C. to 60° C. for a second period of time sufficient for the production of said alcohol or alcohol precursor; andoptionally, recovering said alcohol or alcohol precursor from the cells and / or spent culture media.wherein said genetically modified live ethanologenic organism comprises at least one of the following polynucleotide sequences in its genome:i. at least one endoglucanase (cen-like) polynucleotide sequence selected from the group consisting of an endoglucanase A-like (cenA-like) polynucleotide sequence, an endoglucanase B-like (cenB-like) polynucleotide sequence and an endoglucanase C-like (cenC-like) polynucleotide sequence;ii. an exoglucanase (cex-like) polynucleotide sequence; andiii. a β-glucosidase 1 (bgl1) polynucleotide sequence;wherein said source of cellulose has a lignin content of at most 1 wt. % and a hemicellulose content of at most 15 wt. %, and wherein said live ethanologenic organism belongs to a genus is selected from the group consisting of: Aspergillus, Mucor, Zymomonas, Escherichia, Clostridia, Bacillus, and Pseudomonas.

This disclosure relates to combination therapies comprising anti-Pseudomonas Psl and PcrV binding molecules and related compositions, for use in prevention and treatment of Pseudomonas infection.