47 results about "Brachymonas" patented technology

The invention discloses a denitrification phosphorus removal bacterium as well as a screening method and application efficiency thereof. According to identification, the denitrification phosphorus removal bacterium belongs to pseudomonas, is pseudomonasaeruginosa and is named H-hrb02 and collected in the China General Microbiological Culture Collection Center with the collection number CGMCC NO.5940 on February 27, 2012. An H-hrb02 bacillus is a non-fermented Gram-negative bacterium as well as an obligate aerobe (except that in a nitrate culture medium), grows in the temperature range of 25-42 DEG C, preferably grows at the temperature of 25-30 DEG C, does not grow at the temperature of 4 DEG C particularly, but can grow at the temperature of 42 DEG C. The bacillus is 1.5-5.0 mu m in length and 0.5-1mu m in width, is cue-stick-shaped or linear, and is arranged pairwise or in the form of a short chain; and one end of the bacillus is monotrichous and is free from spores. In a denitrification phosphorus removal strain H-hrb02 provided by the invention, a plurality of organic matters can be taken as carbon sources. After being activated and cultured, the strain (H-hrb02) has excellent denitrification and phosphorus removal performances and can be applied to a sewage biological nitrogen and phosphorus removal process, the phosphorus absorption rate can be up to 92 percent, and the nitrogen removing rate is up to 87.7 percent.

This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that produces itaconate or trans-aconitate. The disclosure further provides methods for producing itaconate or trans-aconitate using a genetically-modified bacterium from the genus Pseudomonas.

This application relates to the modulation of the flora of bacteria in an environment by inhibiting the quorum sensing of a specific bacteria in said environment by administering an effective amount of a quorum sensing control composition comprising at least one quorum sensing control agent, which is an adsorbent / catalytic inhibitor for a QS signal molecule, such as, N-acyl homoserine lactones(AHL), pseudomonas quinolone signal (PQS), and autoinducer-1 (AI-1), autoinducer-2 (AI-2) type of quorum sensing molecules. Quorum sensing control agents include a sorbent material, sorbent mineral or non-porous mineral such as, for example, phyllosilicate clays, silica, calcite, zeolites, diatomaceous earth, smectite, activated carbon, a nanoparticle or a combination of any of the foregoing. Methodsinclude inhibiting the spoilage of food stuffs and preventing vibriosis in fish or shell fish.

The invention discloses a denitrification phosphorus removal bacterium as well as a screening method and application efficiency thereof. According to identification, the denitrification phosphorus removal bacterium belongs to pseudomonas, is pseudomonasaeruginosa and is named H-hrb02 and collected in the China General Microbiological Culture Collection Center with the collection number CGMCC NO.5940 on February 27, 2012. An H-hrb02 bacillus is a non-fermented Gram-negative bacterium as well as an obligate aerobe (except that in a nitrate culture medium), grows in the temperature range of 25-42 DEG C, preferably grows at the temperature of 25-30 DEG C, does not grow at the temperature of 4 DEG C particularly, but can grow at the temperature of 42 DEG C. The bacillus is 1.5-5.0 mu m in length and 0.5-1mu m in width, is cue-stick-shaped or linear, and is arranged pairwise or in the form of a short chain; and one end of the bacillus is monotrichous and is free from spores. In a denitrification phosphorus removal strain H-hrb02 provided by the invention, a plurality of organic matters can be taken as carbon sources. After being activated and cultured, the strain (H-hrb02) has excellent denitrification and phosphorus removal performances and can be applied to a sewage biological nitrogen and phosphorus removal process, the phosphorus absorption rate can be up to 92 percent, and the nitrogen removing rate is up to 87.7 percent.

The present invention generally provides methods to generate broad-spectrum resistance to Xanthomonas pathogenic bacteria in plants. The invention relates to nucleic acid sequences identified, which are associated with broad spectrum disease resistance including Xa1, an NBS-LRR (Nucleotide Binding Site-Leucine-Rich Repeats) type R gene in rice (SEQ ID NOS: 1 and 2) and Xa1 homolog gene, Xa2, (SEQ ID NOS: 3 and 4) and homologs thereof. Further, novel iTALE (interfering transcription activator-like effectors), have also been identified, for example, iTAL3a (SEQ ID NOS: 5 and 6) and iTAL3b (SEQ ID NOS: 7 and 8) and homologs thereof. Modulation of these proteins can improve disease resistance in plants.

Disclosed is a PNA probe that includes a nucleobase sequence suitable for the detection, identification and / or quantitation of Pseudomonas (sensu stricto). In one embodiment, the PNA probe is complementary to a target sequence of 23S rRNA or rDNA from all species of the genus Pseudomonas. The invention has a wide range of important uses including detecting Pseudomonas in a sample of interest.

The invention relates to the technical field of sewage treatment, in particular to a composite microbial strain for degrading "triphenyl" waste gas and a preservation and activation method thereof. The complex microbial species include Bacillus, Pseudomonas, Streptococcus, Rhodococcus and Brevibacterium. The preservation method comprises the following steps: A1, inoculating the compound microbial strains for degrading "triphenyl" waste gas in a sterile enrichment medium at an inoculum amount of 9-11%, and cultivating by shaking to obtain a bacterial liquid; A2, taking the bacterial liquid , protective agent and active stabilizer, mix well and store at -78-82°C. The composite microbial strain of the present invention can efficiently degrade "triphenyl" waste gas, the preservation method is simple and easy to control, and the cost is low, and the activation of the composite microorganism after preservation can maintain a good "triphenyl" degradation ability, which can be benzene series The practical engineering application of waste gas biological treatment can quickly provide microbial strains with high activity to degrade benzene series.

A recombinant microorganism of the genus Escherichia, comprises a genetic modification that increases expression of a nosZ gene encoding NosZ, which is a nitrous oxide reductase, in the recombinant microorganism, wherein the recombinant microorganism comprises a nosR gene encoding NosR, a nosD gene encoding NosD, a nosF gene encoding NosF, a nosY gene encoding NosY, and an apbE gene encoding ApbE, and wherein the nosR gene, the nosD gene, the nosF gene, the nosY gene and the apbE gene are derived from a microorganism of the genus Pseudomonas, the genus Paracoccus, or a combination thereof.

A process for treatment of hydrocarbon refinery wastewater producing a low bio-sludge, the process including utilizing microbial consortia comprising at least one species of Pseudomonas and at least one species of Bacillus in a ratio of 10:1 to 1:10. The species of Pseudomonas and species of Bacillus have constitutive expression of at least one hydrocarbon degrading gene. The species of Pseudomonas are selected from the group consisting of Pseudomonas stutzeri (MTCC 25027), Pseudomonas aeruginosa (MTCC 5389), Pseudomonas aeruginosa strain IOC DHT (MTCC, 5385), Pseudomonas putida IOCR1 (MTCC 5387), Pseudomonas putida IOC5a1 (MTCC 5388) and a mutant thereof. The species of Bacillus are selected from the group consisting of Bacillus subtilis (MTCC 25026), Bacillus substilis (MTCC 5386), Bacillus thermoleovorans (MTCC 25023), Bacillus stearothermophilus (MTCC 25030), Lysinibacillus sp. (MTCC 25029), Lysinibacillus sp. (MTCC 5666) and a mutant thereof. The microbial consortia is used in concentration of at least 102 cfu / ml.

The invention relates to a strain of Brachymonas sp. LM-R isolated from waste gas leaching waste water sediment in a spray booth and its application in the treatment of waste water containing toluene or xylene. Specifically, the invention discloses a Brachymonas sp. LM‑R isolated from the bottom sludge of spray paint wastewater, which is Brachymonas sp., preservation number: CGMCC No.17167, and the strain 16S The rDNA gene sequence is shown as SEQ ID NO.1, which can be used to treat waste water containing toluene and / or xylene.

Present invention relates to the in vitro use of the level or concentration in a salivary or breath sample of bacteria belonging to the Alloprevotella, Prevotella, Campylobacter, Rothia, Catonella, Porphyromona, Fretibacterium genus, or any combination thereof, for the diagnosis of carcinomas or epidermoid cancers, especially epidermoid cancer of the head and neck, in a patient, or to obtain useful data that allow such a diagnosis.

The invention provides a bacterial strain of the genus Alteromonas and its application. The bacterial strain is screened from the algae liquid of Isoflagellates and can promote the growth of Isoflagellates. The strain of the genus Alteromonas provided by the present invention is named Alteromonas sp., the strain number is NBU-A-0001, the preservation number is CCTCC M 2020010, and the preservation date is January 3, 2020. The depository unit is China Center for Type Culture Collection, the address is Wuhan, Wuhan University. The Alteromonas bacterial strain obtained by screening in the present invention can promote the growth of the isochrysis globus, thereby effectively expanding the yield of the isochrysis globus and meeting the requirement of the isochrysis globus as feed.

The invention discloses an Aeromonas strain R1, a preparation method and an application in algae-degrading microcystin degradation. A strain R1 was screened from the rotting water bloom in Jinghu Lake. This strain has strong cadmium tolerance, algae lysis and degradable microcystin. According to 16S rDNA sequence comparison analysis, R1 belongs to Aeromonas Fungi (Aeromonas). The strain against Cd 2+ Well tolerated at 50mg / LCd 2+ It can still grow normally in the culture medium; bacteria dissolve Microcystis aeruginosa by secreting extracellular substances, and this substance is resistant to high temperature, non-nucleic acid and polysaccharides and other substances; at 0.1mg / L Cd 2+ In the presence of the presence of bacteria, the ability of bacteria to dissolve algae was enhanced; when the initial concentration of microcystin was 1.84mg / L, the degradation rate was 43.5% within 7 days.

The present invention provides a new strain of oil-degrading bacteria-Aeromonas genus, Aeromonas allosaccharophila CY01 (Aeromonas allosaccharophila CY01) and the application of the strain. The bacterial strain of the present invention can rapidly multiply in the aerobic pool of the sewage treatment equipment, become a dominant bacterium, continuously secrete lipase, and degrade animal and vegetable oils.

The invention discloses an intestinal flora for diagnosing the sarcopenia of a maintenance hemodialysis patient. The intestinal flora at least comprises Megamonas. The relative abundance of Megamonas of the MHD sarcopenia patient is remarkably reduced (P is smaller than 0.001). The diagnosis value of Megamonas on MHD sarcopenia is represented by ROC and AUC, AUC is 0.787, the sensitivity is 0.867, the specificity is 0.800, and it is shown that Megamonas has high diagnosis efficiency on MHD sarcopenia.

The present invention provides a composition comprising an isolated polypeptide having non-specific nuclease (NSN) activity for degrading nucleic acids comprising or consisting of at least 70% amino acid sequence identity to SEQ ID NO:2, wherein said polypeptide has NSN activity in a solution of about 4 DEG C, and a cation complexing agent such as EDTA. Said composition may have preferentially a neutral to basic pH. A kit comprising said composition and a method using said composition are also disclosed.

The invention provides an identification plate for Gram-negative aerobic bacteria. The combination of 47 carbon sources and nitrogen sources on the identification plate can identify more than 300 kinds of Gram-negative bacteria, can identify various bacteria such as negative Enterobacteriaceae, Vibrionaceae, pseudomonas, Acinetobacter, stenotrophomonas, negative fastidious bacteria, and so on, can obtain an identification result without a supplementary experiment, and has simple operation and accurate results, so the identification plate has higher clinical application value. The invention provides a method for preparing the identification plate for the Gram-negative aerobic bacteria.