18 results about "Brachymonas" patented technology

This invention discloses a heterotrophic-aerobic denitrifying bacterium C-3 and its applications. The heterotrophic-aerobic denitrifying bacterium C-3 is deposited at the China General Microbiological Culture Collection Center (CGMCC), classified as *Brevundimonas naejangsanensis*, with accession number CGMCC No. 31729, and deposited on August 23, 2024. The heterotrophic-aerobic denitrifying vesicular *Brevundimonas naejangsanensis* strain C-3 provided by this invention exhibits excellent nitrate nitrogen removal efficiency (100%) under aerobic conditions using sodium acetate as a carbon source, a C / N mass ratio of 10, a pH of 7, a temperature of 30℃, and a rotation speed of 200 r / min. In actual aquaculture wastewater, it demonstrates excellent removal effects on nitrate nitrogen, nitrite nitrogen, ammonia nitrogen, and total nitrogen. Furthermore, this strain exhibits high safety and has significant application value in treating aquaculture wastewater.

The invention belongs to the technical field of biomedicine, and particularly relates to a flora marker for predicting colorectal cancer liver metastasis and application of the flora marker. According to the present invention, the bacterial group marker is the combination of Acinetobacter, Streptococcus, Pseudomonas, Brevundimonas and Sphingomonas, and the bacterial group marker combination provides extremely high specificity (95.29%) and sensitivity (91.43%) when the early liver metastasis of colorectal cancer is predicted, such that the liver metastasis risk of the patient can be accurately assisted in the determination, and the early liver metastasis risk of the colorectal cancer can be accurately determined; the method has the advantages that reliable basis is provided for clinical decision making, the colorectal cancer liver metastasis can be predicted only by taking the abundance of the specific bacteria as an input index, the method is not limited by specific measurement means, and dependence on a detection technology is greatly reduced.

The invention relates to a Pseudomonas sp. strain for use in the production of a recombinant protein characterised in that said strain comprises a nucleotide sequence encoding a phi15 RNA polymerase. The invention further relates to a plasmid, capable of integrating or replicating in Pseudomonas sp., comprising a phi15 promoter sequence operably linked to a nucleotide comprising one or more restriction sites for the insertion of a nucleotide sequence encoding a recombinant protein, or operably linked to a nucleotide sequence encoding a recombinant protein.

The present disclosure relates to bacteriophages and compositions capable of infecting and killing Pseudomonas, and use of the same for treating Pseudomonas, e.g. Pseudomonas aeruginosa, bacterial infections.

The application provides a method for regenerating activated carbon by microorganisms. The method uses a regenerating bacteria solution composed of Beijerinckia sp. (LM-W, preservation number: CGMCC No. 17168) and / or Brachymonas sp. (LM-R, preservation number: CGMCC No. 17167) obtained by separation, purification and screening from the bottom sludge of paint spraying wastewater, and directly regenerates saturated or unsaturated activated carbon. Without any manual operation intervention and chemical reagent addition, the method can automatically decompose and desorb the organic matters in the saturated or unsaturated activated carbon, and further removes the inorganic matters and impurities in the pores of the activated carbon through ultrasonic high-frequency cleaning in a subsequent process, thereby further improving the regeneration rate of the activated carbon. The method for regenerating activated carbon by microorganisms has the advantages of simple system equipment, short time, low energy consumption, high economic benefit, environmental protection, no pollution, strong stability, large treatment capacity, no need to add any activating agent, high regeneration rate, and the like, and can overcome many defects of traditional methods for regenerating activated carbon by microorganisms.

The problem to be solved by the present invention is to provide an extract of bacteria of the genus Sphingomonas, which may be used as an inhibitor of the kallikrein-kinin system and can reduce the occurrence of redness.SOLUTION: The present invention relates to an extract of bacteria of the genus Sphingomonas, to a composition comprising at least one bacterium of the genus Sphingomonas or said extract, to the uses thereof and to the implementation thereof in a cosmetic method.SELECTED DRAWING: None

The invention provides a pseudomonas bacterium composition, a complex microbial inoculant and application of the pseudomonas bacterium composition and the complex microbial inoculant, and belongs to the technical field of microorganisms. The invention provides a Pseudomonas bacteria combination, which comprises Pseudomonas bacteria JF1 and Pseudomonas hutmensis JF2, the Pseudomonas bacteria JF1 and the Pseudomonas hutmensis JF2 are growth-promoting bacteria screened from rhizosphere soil of rhizoma corydalis, and the Pseudomonas bacteria JF1 and the Pseudomonas bacteria JF2 have the capabilities of dissolving phosphorus and producing siderophores, can be used as growth promoting agents in a synergistic manner to promote the growth of rhizoma corydalis seedlings, and can be used for promoting the growth of rhizoma corydalis seedlings. The method has a remarkable effect of promoting accumulation of the total alkaloid content in rhizoma corydalis tubers, can be used for artificial cultivation of rhizoma corydalis, provides a theoretical basis for improving the yield and quality of rhizoma corydalis, and has important guiding significance for reasonably and effectively utilizing microorganisms to improve the quality and yield of Qinshi medicine-rhizoma corydalis.

This invention relates to the field of microbial technology, and more particularly to a broad-spectrum Aeromonas hydrophila phage and its applications. The Aeromonas hydrophila phage AhP2505006 is classified as follows: Aeromonas hydrophila Phage AhP2505006, with accession number CCTCC NO: M20251307, exhibits cross-species lytic activity. It can effectively lyse *Aeromonas*, *Vibrio parahaemolyticus* and *Vibrio alginolyticus*, and *Citrobacter freundii*. It also possesses excellent targeting ability against multidrug-resistant strains and broad host spectrum characteristics, achieving a lysis rate of up to 80% against multidrug-resistant *Aeromonas hydrophila*. In the prevention and control of diseases caused by various pathogens, or in practical scenarios facing the limitations of traditional antibiotics and highly specific bacteriophages, it has become a highly promising solution.

Disclosed herein are compositions comprising A) compound of Formula One (F1) or Formula Two (F2); and B) biological agent selected from the group consisting of Bacillus species, Baclovirus species, Pseudomonas species, Beauveria species, Mycobacterium species, Mycorrhizal fungi, Trichoderma species, and Metarhizium species.

This invention belongs to the fields of bioinformatics and medical testing technology, specifically relating to a combination of biomarkers related to the postoperative prognosis of colorectal cancer and their application and prediction system. This invention, through 16S rRNA gene sequencing of tumor mucosal samples from colorectal cancer patients, combined with long-term postoperative follow-up data and screening, found that *Peptostreptococcus*, *Fusobacterium*, *Bacteroides*, *Porphyromonas*, and *Prevotella* are closely related to postoperative recurrence of colorectal cancer, with an AUC of 0.81 for assessing postoperative recurrence. Based on this, this invention conducts non-targeted metabolomics analysis, finding that combining alanine glutamate, putrescine, arginine, histidine, and sebacic acid with the above microbial biomarkers can improve the AUC to 0.91, significantly enhancing accuracy, specificity, and precision. This enables prognostic stratification of recurrence-free survival in colorectal cancer patients, demonstrating higher predictive efficacy and stability.

This invention provides a Pseudomonas spp. bacterial composition, a compound bacterial agent, and their applications, belonging to the field of microbial technology. This invention provides a Pseudomonas spp. bacterial composition, comprising Pseudomonas spp. bacteria (… Pseudomonas arcuscaelestis JF1 and ( Pseudomonas hutmensis JF2 and JF2 are growth-promoting bacteria screened from the rhizosphere soil of Corydalis yanhusuo. Pseudomonas bacteria JF1 and JF2 have the ability to solubilize phosphorus and produce iron carriers. As growth promoters, they can be used synergistically to promote the growth of Corydalis yanhusuo seedlings. In particular, they have a significant effect on promoting the accumulation of total alkaloids in Corydalis yanhusuo tubers. They can be used in the artificial cultivation of Corydalis yanhusuo, providing a theoretical basis for improving the yield and quality of Corydalis yanhusuo. They have important guiding significance for the rational and effective use of microorganisms to improve the quality and yield of "Qin medicine"—Corydalis yanhusuo.

The invention provides a pseudomonas bacterium, a microbial inoculum and application of the pseudomonas bacterium and the microbial inoculum, and belongs to the technical field of microorganisms. The invention provides a strain of Pseudomonas aagonensis JF3, and the preservation number of the Pseudomonas aagonensis JF3 is CGMCC (China General Microbiological Culture Collection Center) No. 34848. In the invention, the pseudomonas bacterium JF3 is a growth-promoting bacterium screened from rhizosphere soil of rhizoma corydalis, has the capabilities of fixing nitrogen, dissolving phosphorus and producing siderophores, can be used as a growth promoting agent to promote the growth of overground parts of rhizoma corydalis seedlings, and particularly has a remarkable effect of promoting accumulation of total alkaloid content in rhizoma corydalis tubers; the method can be used for artificial cultivation of the corydalis tuber, provides a theoretical basis for improving the yield and quality of the corydalis tuber, and has important guiding significance for reasonably and effectively utilizing microorganisms to improve the quality and yield of Qinshi medicine-corydalis tuber.

The invention belongs to the technical field of environmental microbiology, is applied to biodegradation of residual 2-methyl-4-chlorophenoxyacetic acid in the environment, and particularly relates to a 2-methyl-4-chlorophenoxyacetic acid degrading strain and application thereof. The Sphingobium sp. S-6 separated from soil belongs to sphingomonas and is preserved in the China Center for Type Culture Collection, the preservation date is April 2, 2025, the preservation number is CCTCC NO: M2025674, and when the temperature is 16-30 DEG C and the pH value is 4-8, the Sphingobium sp. S-6 has a good degradation effect on chipton and has high environmental adaptability, and the Sphingobium sp. S-6 can be used for degrading chipton. An efficient degradation strain resource is provided for elimination and control of residual 2-methyl-4-chlorophenoxyacetic acid in the environment, and important theoretical and application values are achieved.

A method of producing an alcohol or alcohol precursor from a cellulosic material and a genetically modified live ethanologenic organism, wherein the method comprises the steps of:exposing said genetically modified live ethanologenic organism to a culture media with a pH of between 2 and 9 thereby creating an incubation mixture;exposing said incubation mixture to a source of cellulose;incubating said genetically modified live ethanologenic organism in said incubation mixture with said source of cellulose under aerobic and / or anaerobic conditions at a temperature ranging from 0° C. to 60° C. for a first period of time sufficient for the degradation of cellulose, thereby creating a degraded cellulose mixture;optionally, incubating said genetically modified live ethanologenic organism in said degraded cellulose mixture under aerobic and / or anaerobic conditions at a temperature ranging from 0° C. to 60° C. for a second period of time sufficient for the production of said alcohol or alcohol precursor; andoptionally, recovering said alcohol or alcohol precursor from the cells and / or spent culture media.wherein said genetically modified live ethanologenic organism comprises at least one of the following polynucleotide sequences in its genome:i. at least one endoglucanase (cen-like) polynucleotide sequence selected from the group consisting of an endoglucanase A-like (cenA-like) polynucleotide sequence, an endoglucanase B-like (cenB-like) polynucleotide sequence and an endoglucanase C-like (cenC-like) polynucleotide sequence;ii. an exoglucanase (cex-like) polynucleotide sequence; andiii. a β-glucosidase 1 (bgl1) polynucleotide sequence;wherein said source of cellulose has a lignin content of at most 1 wt. % and a hemicellulose content of at most 15 wt. %, and wherein said live ethanologenic organism belongs to a genus is selected from the group consisting of: Aspergillus, Mucor, Zymomonas, Escherichia, Clostridia, Bacillus, and Pseudomonas.

The present invention provides a composition that extends lifespan and / or reduces or delays the onset of at least one age-related symptom or condition in a subject. In some embodiments, a composition comprises at least one bacterial strain or an extract or component thereof and an excipient. In some embodiments, at least one bacterial strain comprises the genus Gluconobacter, Acetobacter, Gluconacetobacter, Acidimonas, Plurashibacter, Asia, Granobacter, Cozecia, NeoAsia, NeoKacia, Gulgarobacter, Granobacter, Cozecia, Chrysanthemum, or a combination thereof. The invention also relates to a method for preparing the bacterial strain. The method includes selecting at least one bacterial strain from the bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, and selecting at least one bacterial strain. Methods of making and using the compositions disclosed herein are also provided.

To provide a novel method for producing heme iron-containing cytochrome c peroxidase.SOLUTION: A method for producing cytochrome c peroxidase includes: a PHB accumulation step of aerobically culturing a halophilic bacterium belonging to the genus Halomonas in the medium for 10 hours or more to cause the halophilic bacterium to accumulate poly-3-hydroxybutyrate; a 3HB secretion step of microaerobically culturing the halophilic bacterium in the medium while adjusting the pH to cause the poly-3-hydroxybutyrate accumulated within the halophilic bacterium to be secreted into the medium as 3-hydroxybutyrate; and a recovery step of recovering the cytochrome c peroxidase from the medium. The pH during microaerobic culture is adjusted to 9.0 or more and 12.0 or less.SELECTED DRAWING: None

The present invention relates to a bacterial strain of Pseudomonas shirazensis CECT 31128, a microorganism of the group of Gram-negative bacteria, of the genus Pseudomonas, which is isolated from the rhizosphere of Nicotiana glauca, its total metabolic liquid (TML) and its metabolic liquid vehiculised in silver nanoparticles (ML-AgNP), stimulants of growth and yield in crop production, stimulants of nitrogen, phosphorous, potassium, sulphur, calcium, magnesium, boron, manganese, zinc and iron absorption in plants, stimulants of adaptive metabolism against abiotic stress and enhancers of photosynthesis, under normal conditions and under water stress conditions, as well as stimulants of secondary metabolism of terpenes and phenolic compounds of pharmacological and food interest. Both the bacterial strain and the metabolic products thereof can be used as the basis for various preparations intended to enhance plant growth and production, as well as within the field of organic biofertilisers to improve mineral nutrition with respect to the indicated nutrients and improve adaptation to abiotic stress.