53 results about "Brachymonas" patented technology

The invention provides chimeric proteins comprising a non-toxic Pseudomonas exotoxin A sequence and a Type IV pilin loop sequence, wherein the Type IV loop sequence is inserted within the non-toxic Pseudomonas exotoxin A. The invention also provides polynucleotides encoding the chimeric proteins, and compositions comprising the polynucleotides or the chimeric proteins. The invention also provides methods for using the chimeric proteins, polynucleotides and compositions of the invention.

The invention discloses a denitrification phosphorus removal bacterium as well as a screening method and application efficiency thereof. According to identification, the denitrification phosphorus removal bacterium belongs to pseudomonas, is pseudomonasaeruginosa and is named H-hrb02 and collected in the China General Microbiological Culture Collection Center with the collection number CGMCC NO.5940 on February 27, 2012. An H-hrb02 bacillus is a non-fermented Gram-negative bacterium as well as an obligate aerobe (except that in a nitrate culture medium), grows in the temperature range of 25-42 DEG C, preferably grows at the temperature of 25-30 DEG C, does not grow at the temperature of 4 DEG C particularly, but can grow at the temperature of 42 DEG C. The bacillus is 1.5-5.0 mu m in length and 0.5-1mu m in width, is cue-stick-shaped or linear, and is arranged pairwise or in the form of a short chain; and one end of the bacillus is monotrichous and is free from spores. In a denitrification phosphorus removal strain H-hrb02 provided by the invention, a plurality of organic matters can be taken as carbon sources. After being activated and cultured, the strain (H-hrb02) has excellent denitrification and phosphorus removal performances and can be applied to a sewage biological nitrogen and phosphorus removal process, the phosphorus absorption rate can be up to 92 percent, and the nitrogen removing rate is up to 87.7 percent.

Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.

The invention discloses salt-tolerant denitrifying bacteria, a strain preparation method thereof and an application of the bacteria, and belongs to the field of environmental microorganisms. The bacteria are named as Halomonas stevensii and belong to anaerobic denitrifying bacteria strains, and the collection number of the denitrifying bacteria is CGMCC NO. 15311. The Halomonas stevensii has highsalt-tolerant and denitrification characteristics, denitrification rate reaches 98% or more, high-concentration nitrate nitrogen in water can be reduced into harmless nitrite-free nitrogen under the condition of sodium chloride salinity lower than 10%, the denitrifying bacterium is applied into a denitrifying denitrification reactor, denitrification is effective and stable, secondary pollution isavoided, a start period can be shortened, and the denitrifying bacteria can be widely applied to denitrification treatment of industrial wastewater.

The invention provides an identification plate for Gram-negative aerobic bacteria. The combination of 47 carbon sources and nitrogen sources on the identification plate can identify more than 300 kinds of Gram-negative bacteria, can identify various bacteria such as negative Enterobacteriaceae, Vibrionaceae, pseudomonas, Acinetobacter, stenotrophomonas, negative fastidious bacteria, and so on, can obtain an identification result without a supplementary experiment, and has simple operation and accurate results, so the identification plate has higher clinical application value. The invention provides a method for preparing the identification plate for the Gram-negative aerobic bacteria.

This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that produces itaconate or trans-aconitate. The disclosure further provides methods for producing itaconate or trans-aconitate using a genetically-modified bacterium from the genus Pseudomonas.

The invention discloses a denitrification phosphorus removal bacterium as well as a screening method and application efficiency thereof. According to identification, the denitrification phosphorus removal bacterium belongs to pseudomonas, is pseudomonasaeruginosa and is named H-hrb02 and collected in the China General Microbiological Culture Collection Center with the collection number CGMCC NO.5940 on February 27, 2012. An H-hrb02 bacillus is a non-fermented Gram-negative bacterium as well as an obligate aerobe (except that in a nitrate culture medium), grows in the temperature range of 25-42 DEG C, preferably grows at the temperature of 25-30 DEG C, does not grow at the temperature of 4 DEG C particularly, but can grow at the temperature of 42 DEG C. The bacillus is 1.5-5.0 mu m in length and 0.5-1mu m in width, is cue-stick-shaped or linear, and is arranged pairwise or in the form of a short chain; and one end of the bacillus is monotrichous and is free from spores. In a denitrification phosphorus removal strain H-hrb02 provided by the invention, a plurality of organic matters can be taken as carbon sources. After being activated and cultured, the strain (H-hrb02) has excellent denitrification and phosphorus removal performances and can be applied to a sewage biological nitrogen and phosphorus removal process, the phosphorus absorption rate can be up to 92 percent, and the nitrogen removing rate is up to 87.7 percent.

The invention relates to a strain of brachymonas sp. LM-R separated from waste gas leaching wastewater bottom mud in a spray paint room and application of the brachymonas sp. LM-R in the treatment ofwastewater containing methylbenzene or xylene. Specifically, the invention further discloses brachymonas sp. LM-R separated from spray paint wastewater bottom mud, namely the brachymonas sp., and thepreservation number is CGMCC No.17167. The gene sequence of the strain 16S rDNA is shown in SEQ ID NO.1, and the strain 16S rDNA can be used for treating the wastewater containing the methylbenzene orxylene.

The invention discloses a pseudomonas strain and application thereof. The strain is preserved in China Center for Type Culture Collection on July 24th, 2020, and the preservation number is CCTCC NO: M2020346. The strain can prevent and treat various plant soil-borne diseases, particularly has a remarkable prevention and treatment effect on plant nematode diseases and soybean root rot, and lays a foundation for research and development of biological prevention and treatment of the soil-borne diseases; and the strain can also promote plant growth, is beneficial to promoting agricultural sustainable development, and has a good application prospect.

The invention provides a composite strain biological fluidized bed membrane reactor, which comprises a plurality of reaction tanks, a biological membrane carrier and an aeration mechanism, and is characterized in that the biological membrane carrier is provided with composite strains, and the composite strains comprise composite oil removing bacteria, composite phosphorus accumulating bacteria, composite denitrifying bacteria and composite desulfurizing bacteria. Preferably, the composite oil removing bacteria comprise bacillus, saccharomycetes and micrococcus, the composite phosphorusaccumulating bacteria comprise aeromonas, pseudomonas and acinetobacter, the composite denitrifying bacteria comprise nitrosomonas and nitrobacter, and the composite desulfurizing bacteria comprise thiobacillus, pseudomonas and acinetobacter. By the adoption of the above scheme, petroleum, gasoline hydrocarbons, phosphorus-containing substances, ammonia nitrogen, nitrites and sulfides in water can be wellremoved, rapid establishment of nitrifying bacteria, phosphorusaccumulating bacteria and desulfurization bacteria in a sewage system is promoted, and the effluent quality is improved.

The invention discloses a mixed bacterial flora for degrading fumonisin B1 and a crude enzyme preparation thereof. The mixed bacterial flora for degrading fumonisin B1 comprises the following components: Pseudomonas (Pseudomonas), Chaetomonas (Comamonas) and Sphingobacterium (sphingobacterium), wherein the total effective viable number of the mixed microflora accounts for more than 90% of the total number of the mixed microflora. The mixed bacterial flora degrading fumonisin B1 of the invention is amplified and cultured in MM culture medium containing sucrose, the bacterial body obtained by centrifugation is broken by ultrasonic wave, and the supernatant is lyophilized to obtain crude enzyme preparation. The mixed bacterial flora degrading fumonisin B1 of the invention is amplified and cultured in MM culture medium containing sucrose. The fumonisin B1 degradation rate of the mixed microflora and the crude enzyme preparation thereof obtained by the invention is above 90% in 24 hours. The mixed microbial flora and its crude enzyme preparation have the characteristics of simple fermentation and low production cost, and have broad application prospects for detoxification of fumonisin B1 in feedstuffs.

The invention discloses a microbial marker for bovine endometritis. The microbial marker comprises the following microorganisms with remarkably increased abundance: Bacteriodetes, Elusimicrobia, Verrucomicrobia, Spirochaetae and Lentisphaerae. The microbial marker also comprises the following microorganisms with remarkably increased abundance: Akkermansia, biogas bacillus, CF231 and Oscillosporia.The microbial marker also comprises the following microorganisms with remarkably decreased abundance: Ureaplasma urealyticum, Butyrivibrio, Doria, Sphingomonas sp, Rothia and Streptococcus. The invention discloses an application of the microbial marker for bovine endometritis in preparation of a tool for early screening or prediction of bovine endometritis. The applicant performs 16S rRNA gene sequencing on microbial DNA of a uterine mucus sample, compares structural differences of uterine florae between healthy and sick dairy cows, and finds differences in microbial community composition ofthe uterine florae between the healthy and sick dairy cows. The result provides a theoretical basis for better prevention and treatment of clinical endometritis.

The present invention generally provides methods to generate broad-spectrum resistance to Xanthomonas pathogenic bacteria in plants. The invention relates to nucleic acid sequences identified, which are associated with broad spectrum disease resistance including Xa1, an NBS-LRR (Nucleotide Binding Site-Leucine-Rich Repeats) type R gene in rice (SEQ ID NOS: 1 and 2) and Xa1 homolog gene, Xa2, (SEQ ID NOS: 3 and 4) and homologs thereof. Further, novel iTALE (interfering transcription activator-like effectors), have also been identified, for example, iTAL3a (SEQ ID NOS: 5 and 6) and iTAL3b (SEQ ID NOS: 7 and 8) and homologs thereof. Modulation of these proteins can improve disease resistance in plants.

The invention relates to an antibacterial peptide, a composition containing the antibacterial peptide and application. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID No. 1. The antibacterial peptide has antibacterial activity against microorganisms in the genus Staphylococcus, Streptococcus, Enterococcus, Micrococcus, Moraxella, Corynebacterium, Klebsiella, Enterobacter, Serratia, Proteus, Pseudomonas, Morgan morganella, Haemophilus, Flavomonas, Acinetobacter and Propionibacterium.

The present invention discloses a kind of method for preparing L-citrulline by using Aeromonas sp., which is related to the technical field of bioengineering. The said method consists of following steps: fermenting Aeromonas sp. in a culture medium to obtain seed liquid, and the liquid is inoculated into the fermenter at an inoculum size of 1-2% by volume. The initial pH is 3.5, and the pH in the medium is controlled below 4.0 all the time. Then, conducts shaking culture for 15-20 hours to obtain the fermentation broth. The fermentation broth is centrifuged to obtain precipitation solution and supernatant; Use the said supernatant to prepare the substrate solution, and the pH of the substrate solution is controlled at 3-5, and then, add the precipitation solution to react at the temperature of 30-50° C., the reaction time is 5-8 hours. After reaction, the L-citrulline is obtained by the method of concentration and crystallization. The present invention provides a method for preparing L-citrulline with low cost, high L-citrulline content and high purity.

The invention discloses a mixed bacterium agent capable of degrading toluene, xylene and acrylic acid water mixed waste water. The mixed bacterium agent contains brachymonas sp (Brachymonas sp) LM-R and Beijerinckiacase sp (Beijerinckiacase sp) LM-W. Besides, the invention also provides an application of the mixed bacterium agent to degradation of mixed waste water containing toluene, xylene and acrylic acid at the same time. The mixed waste water containing toluene, xylene and acrylic acid is inoculated with the mixed bacterium agent until the inoculum concentration of the brachymonas sp LM-Ris OD<415nm>0.2-0.4, and the inoculum concentration of the Beijerinckiacase sp LM-W is OD<415nm>0.1-0.2, then under an open system, stirring is performed, degradation is performed under the conditionof pH being 6.0-7.0 and temperature being 25-30 DEG C, and the degradation time is at least 24h.

A recombinant microorganism of the genus Escherichia, comprises a genetic modification that increases expression of a nosZ gene encoding NosZ, which is a nitrous oxide reductase, in the recombinant microorganism, wherein the recombinant microorganism comprises a nosR gene encoding NosR, a nosD gene encoding NosD, a nosF gene encoding NosF, a nosY gene encoding NosY, and an apbE gene encoding ApbE, and wherein the nosR gene, the nosD gene, the nosF gene, the nosY gene and the apbE gene are derived from a microorganism of the genus Pseudomonas, the genus Paracoccus, or a combination thereof.

A biofilm disrupting composition for use in treating biofilm-mediated infections due to non-Pseudomonas micro-organisms in the Cystic Fibrosis patient. One embodiment of the composition of the invention comprises at least one biologically acceptable thiol based antioxidant and at least one antibiotic. Another embodiment of the composition of the invention comprises at least one biologically acceptable thiol based antioxidant, at least one enzyme and at least one antibiotic. The invention is also directed to the process of preparing the composition of the invention, the use of the composition for the manufacture of a medicament for disrupting biofilms formed by non-Pseudomonad micro-organisms, and a method of disrupting biofilm formed by non-Pseudomonad micro-organisms in a patient, comprising administering to the patient the composition of the invention.

A process for treatment of hydrocarbon refinery wastewater producing a low bio-sludge, the process including utilizing microbial consortia comprising at least one species of Pseudomonas and at least one species of Bacillus in a ratio of 10:1 to 1:10. The species of Pseudomonas and species of Bacillus have constitutive expression of at least one hydrocarbon degrading gene. The species of Pseudomonas are selected from the group consisting of Pseudomonas stutzeri (MTCC 25027), Pseudomonas aeruginosa (MTCC 5389), Pseudomonas aeruginosa strain IOC DHT (MTCC, 5385), Pseudomonas putida IOCR1 (MTCC 5387), Pseudomonas putida IOC5a1 (MTCC 5388) and a mutant thereof. The species of Bacillus are selected from the group consisting of Bacillus subtilis (MTCC 25026), Bacillus substilis (MTCC 5386), Bacillus thermoleovorans (MTCC 25023), Bacillus stearothermophilus (MTCC 25030), Lysinibacillus sp. (MTCC 25029), Lysinibacillus sp. (MTCC 5666) and a mutant thereof. The microbial consortia is used in concentration of at least 102 cfu / ml.

Present invention relates to the in vitro use of the level or concentration in a salivary or breath sample of bacteria belonging to the Alloprevotella, Prevotella, Campylobacter, Rothia, Catonella, Porphyromona, Fretibacterium genus, or any combination thereof, for the diagnosis of carcinomas or epidermoid cancers, especially epidermoid cancer of the head and neck, in a patient, or to obtain useful data that allow such a diagnosis.

Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

The invention provides a bacterial strain of the genus Alteromonas and its application. The bacterial strain is screened from the algae liquid of Isoflagellates and can promote the growth of Isoflagellates. The strain of the genus Alteromonas provided by the present invention is named Alteromonas sp., the strain number is NBU-A-0001, the preservation number is CCTCC M 2020010, and the preservation date is January 3, 2020. The depository unit is China Center for Type Culture Collection, the address is Wuhan, Wuhan University. The Alteromonas bacterial strain obtained by screening in the present invention can promote the growth of the isochrysis globus, thereby effectively expanding the yield of the isochrysis globus and meeting the requirement of the isochrysis globus as feed.

A bacterium belonging to the genus Pseudomonas such as Pseudomonas aeruginosa is specifically and quantitatively detected using a selection medium for a bacterium belonging to the genus Pseudomonas characterized by containing, in a basal medium, at least one third-generation cephalosporin and at least one antibiotic selected from the group consisting of a first-generation cephalosporin and a glycopeptide-based antibiotic and by a method for detecting a bacterium belonging to the genus Pseudomonas and a method for selecting a bacterium belonging to the genus Pseudomonas through use of said selection medium.

The present invention provides a new strain of oil-degrading bacteria-Aeromonas genus, Aeromonas allosaccharophila CY01 (Aeromonas allosaccharophila CY01) and the application of the strain. The bacterial strain of the present invention can rapidly multiply in the aerobic pool of the sewage treatment equipment, become a dominant bacterium, continuously secrete lipase, and degrade animal and vegetable oils.