94results about How to "Low hemolysis rate" patented technology

The invention discloses an endotoxin adsorbent used in hemoperfusion, and a preparation method thereof. The preparation method comprises the following steps: a porous carrier is prepared; the carrier is polystyrene divinylbenzene resin or polymethyl methacrylate resin, wherein the pore size of the resin is 5-50nm and the specific surface area of the resin is no less than 500m<2>/g; residual double bonds of the carrier resin are subjected to epoxidation modification; and ligands are immobilized. According to the scheme of the invention, a synthesis process route is changed, such that adsorbent harmful substance residue is avoided, and adsorbent adsorption capacity is improved. Specifically, residual double bonds of the adsorption resin are subjected to epoxidation modification, such that the epoxy group amount is increased; with the epoxy groups, hydrophobic tethers are added, such that ligand movement capacity is improved, and the adsorbent is provided with a good endotoxin adsorption effect; and resin pore size and specific surface area are controlled, such that the adsorption amount and removal rate of the adsorbent upon cytokines are ensured.

The invention provides novel synthesis antibacterial peptides and application thereof. The novel synthesis antibacterial peptides are designed and synthesized on the basis of analyzing sequences and structures of natural antibacterial peptides, and the sequences of the novel synthesis antibacterial peptides comprise WYQ-a: ArgArgTrpTrpArg and WYQ-b: PheArgTrpTrpArg. The synthesis antibacterial peptides have the broad spectrum killing activity on gram-positive bacteria and gram-negative bacteria, have higher bactericidal activity than that of the natural antibacterial peptides, and do not have a toxic effect on animal and plant cells. The synthesis antibacterial peptides have broad antibacterial spectra and simple structures, are not needed to be modified and connected and are convenient to synthesize artificially. Experiments prove that: for G+bacteria (the gram-positive bacteria) and G-bacteria (the gram-negative bacteria), the novel synthesis antibacterial peptides have the obvious bacteriostatic and bactericidal activity and do not have hemolysis activity, so the synthesis antibacterial peptides have significant value in the development and application of antibacterial medicines.

The invention discloses a micromolecule synthesized anti-microbial peptide, as well as a preparation method and application thereof. The sequence of a cationic anti-microbial peptide is SQ1: R-W-W-R-F-NH2 (Arg-Arg-Trp-Trp-Arg-NH2), and the preparation method is the solid-phase chemosynthesis method. The micromolecule synthesized anti-microbial peptide has broad-spectrum cytotoxicity to gram-positive bacteria and gram-negative bacteria, has stronger bactericidal activity than a natural anti-microbial peptide, is simple in structure, convenient for artificial synthesis without any modification and connection, has the advantages of small hemolytic toxicity and no poison effects on animal and plant cells, and has an important value in development and application of antimicrobial agents.

The invention belongs to the technical field of membranes, and in particular relates to a modified polyvinylidene fluoride (PVDF) hollow fiber membrane for hematodialysisa, and a preparation method thereof. A PVDF body is modified by using acryloyl morpholine with rejecting performance on protein adsorption and cell adhesion and acrylic acid with high hydrophilic performance to prepare a hollow fiber membrane; the surface of the membrane is subjected to surface modification by L-arginine with an anticoagulation effect; and the anti-pollution performance, the blood compatibility and the anticoagulation performance of the membrane are finally improved, and a modified hollow fiber hemodialysis membrane with high biocompatibility and anticoagulation performance is obtained. The process provided by the invention is simple and can be applied to the field of hemodialysis.

The invention discloses an anticoagulation PVDF flat separation film, also discloses a making method of the anticoagulation PVDF flat separation film, and belongs to the technical field of films. The making method of the anticoagulation PVDF flat separation film comprises the following steps: 1, preparing a polyacrylic acid grafted polyvinylidene fluoride copolymer PVDF-g-PAA; 2, making a PVDF-g-PAA flat separation film; and 3, making an argatroban grafted and modified PVDF-ARG film. A PVDF film is modified by micro-molecular argatroban with a high efficiency anticoagulation effect with acrylic acid as a medium in order to obtain a PVDF separation film material with a good anticoagulation effect. The anticoagulation PVDF flat separation film can obviously prolong the recalcification time of the film, substantially reduces adhesion of platelet erythrocytes on the surface of the film, reduces the hemolysis rate, improves the anticoagulation performance and greatly improves the blood compatibility. The method has the characteristics of simple process and easy industrialization enforcement.

The invention discloses a method for preparing a praseodymium-doped titanium nitride coating on the surface of a medical titanium alloy. The method comprises the following steps: preparing a titanium nitride coating on the surface of the medical titanium alloy by utilizing a magnetron sputtering method; transferring the medical titanium alloy with the titanium nitride coating into a vacuum chamber A of a plasma-immersion-type ion implanter under the condition that a praseodymium target and a sample disk A are respectively arranged at two ends of the inside of the vacuum chamber A; fixing the medical titanium alloy with the titanium nitride coating on the sample disk A; regulating the vacuum degree of the vacuum chamber A under the condition that the praseodymium target is taken as an ion source of a cathode; regulating the rotating speed of the sample disk A and cooling the sample disk A in a water-cooling manner; and then, carrying out praseodymium ion implantation at a room temperature, thereby obtaining the praseodymium-doped titanium nitride coating prepared on the surface of the medical titanium alloy. According to the method disclosed by the invention, the corrosion resistance of the prepared praseodymium-doped titanium nitride coating is obviously superior to that of a common titanium nitride coating; the breeding and the spreading of a large quantity of vascular endothelial cells in the praseodymium-doped titanium nitride coating can be carried out, so that the biological performance of the medical titanium alloy is obviously improved.

The invention discloses a pit-hole composite micro-nano-structure polysaccharide microsphere and a preparation method. The preparation method comprises the following steps: (1) preparing a polysaccharide water solution; (2) adding an emulsifying agent into an oil phase so as to prepare the oil phase containing the emulsifying agent, dropwise adding the polysaccharide water solution into the oil phase containing the emulsifying agent, and stirring for emulsification, so as to obtain a uniform emulsion; (3) dissolving a polyvalent metal cross-linking agent into ultrapure water to prepare a cross-linking agent water solution, dropwise adding the cross-linking agent water solution into the uniform emulsion; and (4) adding n-hexane or petroleum ether into liquid obtained in the step (3), standing until the solution is layered, pouring out an upper oil phase layer, remaining a water phase, cleaning the water phase, and carrying out freeze-drying, so as to obtain the pit-hole composite micro-nano-structure polysaccharide microsphere. The surface of the microsphere is provided with nano-scale pits, and nano-scale fine holes are formed in the surfaces of the pits; the microsphere is uniformin particle size distribution, high in porosity and water absorption rate and strong in adsorbability; and the microsphere has good biocompatibility, and hemostasis can be effectively finished within20s-50s.

The invention provides an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide is designed and obtained through a rational moleculardesign method on the basis of family analogues Pexiganan of the antibacterial peptide magainin derived from the epithelium of xenopus laevis. The antibacterial peptide is an antibacterial peptide Pexi-1 with an amino acid sequence as shown in SEQ ID NO. 1 or an antibacterial peptide Pexi-2 with an amino acid sequence as shown in SEQ ID NO. 2. The antibacterial peptide provided by the invention hasgood structural characteristics, can significantly improve the antibacterial effect on staphylococcus aureus, salmonella and aspergillus flavus, effectively reduces the hemolysis rate and cytotoxicity of chicken erythrocytes, and can be effectively used in animal-related production activities.

The invention provides a freeze-dried preparation containing fosaprepitant and a preparation method of the freeze-dried preparation. The freeze-dried preparation contains an active ingredient and other carriers, wherein the active ingredient is an effective amount for treatment of fosaprepitant dimeglumine, and the other carriers include a solubilizing agent, a complexing agent and a freeze-drying excipient; before freeze-drying, an acidity regulator is used for regulating the pH value of a liquid medicine to be 6.5-9.5; and the solubilizing agent is selected from one or more of polyethylene glycol dodecahydroxyl lithium stearate, hydroxypropyl-beta-cyclodextrin and polyethylene glycol. The freeze-dried preparation containing fosaprepitant, provided by the invention, is stable in property and low in hemolysis rate, and improves the compliance of clinical medication of patients.

The invention discloses an antimicrobial hexapeptide. The antimicrobial hexapeptide has a sequence of Arg-Arg-Trp-Trp-Arg-Trp. The antimicrobial hexapeptide can be used in preparation of drugs for treating or preventing bacterial infection. The invention also provides an antimicrobial hexapeptide derivative. The antimicrobial hexapeptide derivative has a sequence of Arg-Arg-Trp-Trp-Arg-Trp-beta-phenylethylamine. The antimicrobial hexapeptide derivative can be used in preparation of drugs for treating or preventing bacterial infection. The bacterium is drug-resistant acinetobacter baumannii. The artificially designed synthetic antimicrobial hexapeptide and its phenylethylamine-modified product can be conveniently obtained by a solid phase synthesis method. The synthetic antimicrobial peptide and its derivative have broad spectrum killing activity to Gram-positive bacteria and Gram-negative bacteria, and particularly, the derivative exhibits stronger antibacterial activity to drug-resistant acinetobacter baumannii, has very small hemolytic toxicity and can be used in drugs for treatment or prevention of diseases caused by drug-resistant acinetobacter baumannii.

The invention discloses a composite material with a rapid blood coagulation effect and a preparation method thereof and belongs to the technical field of biological medicines. The composite material with the rapid blood coagulation effect is prepared from the following raw materials in parts by mass: 8 to 20 parts of chitosan, 7 to 14 parts of salicylaldehyde, 8 to 16 parts of 2-aminoisonicotinic acid, 1.5 to 3.5 parts of ferrous sulfate, 3 to 5 parts of dopa and 10 to 15 parts of disodium hydrogen phosphate. The prepared composite material with the rapid blood coagulation effect has good product stability and high biological safety, has a remarkable effect on blood coagulation, and is simple in process and easy to industrially produce.

The invention relates to a high-plasticity biodegradable Zn-2Li alloy under a GPa-level high-pressure effect and a preparation method thereof. The high-plasticity biodegradable Zn-2Li alloy comprisesthe following components of, by mass, 2% of Li and the balance Zn. The pressure value of the Zn-2Li alloy is the 1 GPa-5 GPa level. The compressive yield strength of the Zn-2Li alloy is 420-550 Mpa, the compressive ultimate strength is 600-800 Mpa, and ductility is 18%-24%. According to the provided preparation method of the high-plasticity biodegradable Zn-2Li alloy under the GPa-level high-pressure effect, more excellent blood compatibility, more excellent mechanical reliability and more excellent abrasion resistance are achieved.

The invention discloses a fat-soluble drug submicron capsule glucose injection and a preparation method thereof. The invention is characterized in that the capsule material comprises the following components in parts by weight: 15-45 parts of sodium alginate, 2.5-9 parts of poloxamer 188, 1.5-5 parts of silk fibroin, 0.5-3 parts of polyvinyl alcohol and 0.2-3 parts of polyethylene glycol. The preparation method comprises the following steps of: sequentially adding the poloxamer, a silk fibroin aqueous solution, a polyvinyl alcohol aqueous solution, the polyethylene glycol and glucose into a sodium alginate aqueous solution at intervals under stirring, and finally dropwise adding a corresponding fat-soluble drug; adopting an ultrasonic and shearing treatment mode; and finally, carrying outfiltration with a 450nm membrane and damp-heat sterilization to obtain the submicron capsule glucose injection. The preparation process of the submicron capsule glucose injection provided by the invention is simple and economic, the particle size of submicron capsules is controllable and uniform, and the biological safety is high.

The invention relates to a nano drug of photoinduced release of nitric oxide anti-biofilm, and a preparation and application method. A synthesis method includes the following steps: (1) performing oxidation co-deposition on acetylglucosamine and dopamine, obtaining glucosamine through deacetylation, and preparing a cationic monosaccharide modified polydopamine nanomaterial (PDG); (2) using the reducibility of an orthophthalic bisphenol structure on the PDG to in situ reduce HAuCl4 into elemental gold nanoparticles, and synthesizing a glucosamine modified polydopamine@gold hybrid nanomaterial (PDG@Au); (3) blending an NO donor TCF and the PDG@Au in a glass sample bottle, and obtaining a PDG@Au-NO nanomaterial through magnetic stirring and centrifugal washing; and (4) modifying diblock copolymer p(AM-b-APBA) prepared from monomer N-3-acrylamide phenyl boric acid (APBA) and monomer4-acryloyl morpholine (AMP) through RAFT polymerization on a surface of the PDG@Au, and preparing PDG@Au/PBA. The nanomaterial obtained by the synthesis method has a good antibacterial effect and an effect of dissipating biofilm.

The invention relates to a sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel and a method for preparing the same.The method includes steps of carrying out low-temperature plasma surface pre-treatment on a polytetrafluoroethylene artificial blood vessel, vertically or obliquely arranging the polytetrafluoroethylene artificial blood vessel, sealing one end of the polytetrafluoroethylene artificial blood vessel, forming an opening in the other end of the polytetrafluoroethylene artificial blood vessel, keeping the opening upwards, injecting 0.5-2.0 mg/mL silk fibroin solution into the polytetrafluoroethylene artificial blood vessel from the opening, evaporating the silk fibroin solution and coating the silk fibroin solution in the polytetrafluoroethylene artificial blood vessel; turning the polytetrafluoroethylene artificial blood vessel over, keeping the opening upwards, injecting 0.5-2.0 mg/mL silk fibroin solution into the polytetrafluoroethylene artificial blood vessel from the opening, evaporating the silk fibroin solution and coating the silk fibroin solution in the polytetrafluoroethylene artificial blood vessel to obtain a silk fibroin film modified polytetrafluoroethylene artificial blood vessel, and carrying out low-temperature plasma surface sulfonation treatment on the silk fibroin film modified polytetrafluoroethylene artificial blood vessel to obtain the sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel.The sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel and the method have the advantages that the sulfonated silk fibroin film modified polytetrafluoroethylene artificial blood vessel prepared by the aid of the method is good in uniformity and excellent in blood and tissue compatibility and rarely has chap and crack.

The invention relates to a method for preparing immune adjuvant soybean saponin lipotropy derivatives with enhanced activity. The method comprises the following steps: preparing a target product from high-purity soyasaponin Ab, a carbon imine reagent and lauryl amine; detecting the target product by adopting analytical type high performance liquid chromatography (HPLC) and personal digital assistant (PDA) detectors and an evaporative light scattering detector (ELSD); identifying the molecular weight of the target product by using electrospray ionization-mass spectrometry (ESI-MS); carrying out purification and separation by adopting preparative liquid chromatography, so as to obtain lipotropy derivatives AbD of lauryl amine; and analyzing the structure of the target product by adopting nuclear magnetic resonance (NMR). Compared with the prior art, a high-purity modifier AbD is prepared by adopting the method, the molecular weight of the target product is 1603, the yield is 6.94%, the hemolysis ratio is low, the immune adjuvant soybean saponin lipotropy derivatives have potentiation on in vitro immunization of spleen lymphocytes in mice, and meanwhile, the level of four specific antibodies in OVA immune serum in mice is improved.

The invention discloses a digestive tract mucous membrane promotion material. The digestive tract mucous membrane promotion material is prepared by crosslinking genipin and small intestinal submucosa, and the crosslinking index of the small intestinal submucosa crosslinked by genipin is 30-100%. The invention also discloses a preparation method and a use of the digestive tract mucous membrane restoration promotion material. The digestive tract mucous membrane restoration promotion material has the advantages of good acid and pepsin degradation resistance, low hemolytic property, no cytotoxicity and excellent mechanical properties.