3results about How to "Low hemolytic activity" patented technology

This invention belongs to the field of antimicrobial peptide technology, and particularly relates to a broad-spectrum antimicrobial peptide with low hemolytic activity and its applications, as well as a broad-spectrum antibacterial drug. The antimicrobial peptide is named AY6, and its amino acid sequence is shown in SEQ ID NO.1. Antimicrobial peptide AY6 exhibits significant antibacterial effects against both Gram-positive and Gram-negative bacteria, with a bactericidal rate far exceeding that of traditional antibiotics, enabling highly effective responses to bacterial infections. AY6 has low hemolytic activity and good biocompatibility, making it suitable for medical anti-infective applications. The antimicrobial peptide provided in this application has a short sequence, is easy to synthesize chemically, can save on large-scale production costs, and has broad application prospects.

The application provides a preparation of gramicidin and an antibacterial hydrogel preparation of the gramicidin, and discloses medical uses of the gramicidin, and provides a recombinant gramicidin, heterologous expression of the gramicidin is realized by constructing a Pichia pastoris recombinant strain, and the antibacterial activity of the gramicidin is retained, and the method has the advantages of simplicity and rapidness. Meanwhile, the hydrogel preparation of the gramicidin provided by the application shows good potential for treating wound infection of a diabetic mouse, and has the performance of rapid adhesion and hemostasis, lays a foundation for development of a new antibacterial drug, and provides a new direction for treatment of chronic inflammatory wounds and open bleeding.

This invention discloses broad-spectrum antibacterial peptides WP-4 and WP-6, their compositions, and applications, belonging to the fields of antimicrobial peptide technology and biomedicine. This invention obtains a series of antimicrobial peptides by truncating and rationally modifying template peptides to balance the relationship between charge number, hydrophobicity, and amphiphilicity. By measuring antibacterial activity, stability, hemolytic activity, and cytotoxicity, two novel antimicrobial peptides, WP-4 and WP-6, with high activity and low toxicity were obtained, and their amino acid sequences are shown in SEQ ID NO. 5 and SEQ ID NO. 7. The novel antimicrobial peptides obtained by this invention possess broad-spectrum antibacterial activity, good stability under temperature, pH, salt ion, pepsin, and serum conditions, and exhibit no significant hemolytic activity and low cytotoxicity. They are characterized by rapid bactericidal action and low likelihood of inducing drug resistance. Furthermore, the antimicrobial peptides of this invention can enter cells and have a strong inhibitory effect on intracellular bacteria, showing broad application prospects.