Ultra-short peptide Purin-WH for boosting skin repairing as well as preparation method and application thereof

Abstract

The invention discloses an ultra-short peptide Purin-WH for boosting skin repairing as well as a preparation method and application thereof and belongs to the field of biological medicine. The ultra-short peptide Purin-WH is a straight-chain peptide; the amino acid structure of the ultra-short peptide Purin-WH is Val-Val-Pro-Thr-Val-Val-Cys-Pro-Lys; the ultra-short peptide Purin-WH can be synthesized by an automatic peptide synthesizer and is desalted and purified through HPLC anti-phase column chromatography. The ultra-short peptide Purin-WH disclosed by the invention is low in molecular weight, has a simple structure, is ultralow in compounding cost, is capable of obviously quickening the skin wound healing, effectively removing active oxygen and boosting the generation of skin collagen and is beneficial to skin repairing; the ultra-short peptide Purin-WH is free from cytotoxicity and hemolysis; the ultra-short peptide can be used for preparing the drug for repairing and regenerating injured epidermis, such as, the drugs for treating burn, scald and skin ulcer; the ultra-short peptide can be used for replacing the EGF used in the fields of anti-ageing, antioxidant skincare products for repairing skin and stimulating skin collagen hyperplasia; the ultra-short peptide Purin-WH is not limited to such a function.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an ultrashort peptide Purin-WH for promoting skin repair, its preparation method and application. Background technique [0002] The skin is the largest organ covering the surface of the human body, and is easily affected by many factors (such as external physical and chemical factors, changes in hormone levels, mechanical pressure, etc.). At the same time, the skin is the first natural barrier between the body and the outside world. Once damaged, the body will immediately start physiological regulation to repair the skin damage. [0003] Skin injury repair is a complex process that requires the participation of various types of cells, and generally includes three stages: (1) inflammation; (2) wound re-epithelialization, new tissue formation; (3) regeneration tissue structure remodeling. These three stages are interlaced and differentiated from each other, which is a continuous self-re...

AI Technical Summary

Problems solved by technology

It is known that the traditional method of obtaining EGF at first is to separate and extract it from the urine of animals. After 12 procedures, 3000L of human urine is consumed, but the yield is very low.

EGF is extracted from body fluids containing EGF such as saliva, tears, breast milk and plasma, and the yield is also extremely low.

The price per gram is nearly 2 million US dollars, which greatly limits the application of EGF in the field of beauty

If chemical synthesis is used, it should be noted that hEGF contains 3 pairs of disulfide bonds, which will cause great difficulties in synthesis efficiency, and the yield and activity will not be satisfactory

However, biosynthetic methods will not be able to directly guarantee the yield and the accuracy of protein modification.

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