663 results about "Skin wound" patented technology

A compound, as shown in Formula I, is a multi-heterocyclic compound. It has been verified that the compound provided by this invention integrates a novel compound targeting the calcitonin gene-related peptide (CGRP) based on ephedrine. Upon contact with the skin, due to photoactivated cell biological function regulation and the blocking effect of the multi-heterocyclic compound I structure on serotonin receptors, it achieves therapeutic effects on cutaneous vascular malformations and telangiectasia, improves the effectiveness of phototherapy in improving chronic cutaneous vascular diseases, and promotes skin wound healing.

The invention provides application of a turbellarian worm extract in preparation of a product for promoting wound healing, a biological agent for promoting the proliferation activity and / or migration ability of fibroblast NIH3T3, a biological agent for improving the wound healing rate and the decrustation time and a biological agent for improving the collagen fiber deposition type in the wound healing process. The biological preparation is used for promoting regeneration of skin appendage organs in the wound healing process. Wherein the extraction of the turbellarian worm extract comprises the steps of turbellarian worm cleaning, tissue crushing, alcohol extraction, centrifugal separation and concentration drying. The turbellarian worm extract has good capacity of promoting wound healing, and has the effects of effectively improving skin wound healing efficiency, shortening decrustation time, inhibiting scar formation in the wound healing process, promoting regeneration of skin appendage organs in the wound healing process and the like.

The invention relates to the field of cell biology, and particularly discloses an exosome as well as a preparation method and application thereof. The method for preparing the exosome comprises the following steps: (1) introducing nucleotide sequences for coding a signal peptide TPA and an epidermal growth factor into an AAVS1 region of a host cell, such as a sequence shown in SEQ ID No.9 and a sequence shown in SEQ ID No.10, so as to obtain a modified host cell; and (2) collecting the exosome secreted by the modified host cell. The expression level of EGF mRNA in the exosome can be remarkably improved, so that the obtained exosome has a better effect in skin wound treatment.

The invention belongs to the technical field of biological medicine, and relates to hydrogel for promoting wound healing and a preparation method and application thereof.The preparation method comprises the steps that firstly, coriander exosomes are extracted from coriander through a gradient centrifugation and sucrose density gradient method, then the coriander exosomes and a sodium alginate solution are mixed according to the mass-to-volume ratio of 0.5-2 mg: 1 mL, and a hydrogel solution is obtained; and mixing the exosome suspension with a calcium chloride solution according to a volume ratio of (0.5-2): 1, and carrying out crosslinking to obtain the coriander exosome-loaded hydrogel. The coriander exosome-loaded novel hydrogel provided by the invention has good viscoelasticity and biocompatibility, and can be applied to the surface of a skin wound as a dressing; meanwhile, the hydrogel has a coriander exosome slow-release effect, so that the exosome release time is prolonged, and the biological function is better played; by slowly releasing the coriander exosome, the hydrogel can relieve oxidative stress and inflammation of wounds and promote wound repair.

The invention provides an elastin peptide-containing composition as well as a preparation method and application thereof. The elastin peptide-containing composition comprises elastin peptide, camel milk active small molecule peptide and water lily oligopeptide in a mass ratio of 1: (1-3): (1.8-2.5). The elastin peptide, the camel milk active small molecule peptide and the water lily oligopeptide which are obtained through a specific treatment mode are compounded according to a specific proportion, synthesis of aquaporin AQP3 can be promoted, the content of sodium hyaluronate is increased, and the free radical scavenging activity is improved. The composition containing the elastin peptide can quickly and efficiently improve the skin state, repair the skin, delay the skin aging, enhance the skin function and recover the elasticity. The composition containing elastin peptide can promote the proliferation and growth of fibroblasts, improve the cell viability and promote the healing of skin wounds, and is non-toxic to cells.

The invention belongs to the field of high polymer material chemistry, and particularly relates to double-network hydrogel based on sodium alginate / gelatin, a preparation method and dressing. A chemical method of grafting modification is adopted, grafting modification is carried out by designing abundant active groups on side chains of gelatin and sodium alginate, and based on traditional gelatin or sodium alginate hydrogel, not only is the thermal stability of gelatin improved, but also the biological activity of sodium alginate is improved, and the antibacterial property is achieved; the finally prepared SD / GelMA dual-network hydrogel wound dressing not only has excellent mechanical properties, but also has very high biocompatibility and antibacterial activity, and has huge application prospects and potential in the aspect of skin wound healing.

The invention relates to a double-layer dressing for promoting wound healing and inhibiting scars and a preparation method and application thereof.The double-layer dressing comprises a hydrogel layer and a piezoelectric film layer compounded on the hydrogel layer, and the piezoelectric film layer is prepared by mixing an organic piezoelectric material, a ferroelectric material and an organic solvent and then conducting an electrostatic spinning technology; the hydrogel layer is formed by curing a methacrylic anhydride acellular extracellular matrix, methacrylic anhydride catechol chitosan and conductive nanoparticles under the action of a curing agent. The lower skin contact layer of the double-layer dressing is the hydrogel layer, so that the directional growth of fibroblasts is favorably induced; the upper dressing is a piezoelectric material composite ferroelectric material, is beneficial to piezoelectric stimulation re-induction function recovery after final skin repair and guidance of directional growth of wound cells, and has good skin wound induction repair and scar inhibition effects.

The utility model discloses a multidirectional adjustable skin tension reducing device, which belongs to the technical field of skin tension reducing devices, and adopts the technical scheme that the multidirectional adjustable skin tension reducing device comprises two patches, the tops of the patches are fixedly connected with a fixing strip, a plurality of adjusting mechanisms are arranged in the fixing strip; the adjusting mechanism comprises a sliding block, a rubber soft cushion and a pull strip, the bottom of the sliding block makes contact with the top of the patch, the side, close to the sliding block, of the rubber soft cushion is fixedly connected with the sliding block, and the side, close to the rubber soft cushion, of the pull strip penetrates through the rubber soft cushion and is fixedly connected with the surface of the sliding block; the skin tension reducing device solves the problems that roots of braces of most existing skin tension reducing devices are integrated with patches, after the patches are attached to the surfaces of skin wounds, the density of the braces is inconvenient to move and adjust in multiple directions according to needs, the density of the braces is inconvenient to adjust according to different positions of skin tension, use is limited, and the skin tension reducing effect is poor. Therefore, the use effect is influenced.

The invention discloses a shell nacre-derived anti-inflammatory active peptide and application thereof in preparation of a skin repair product. The inhibition rate of the active peptide GEIRYEYF provided by the invention on the NO release amount is concentration-dependent, and the NO release amount is reduced by 54.32 + / -2.13% under the concentration of 200 mu g / mL; in addition, secretion of RAW264.7 macrophage pro-inflammatory cytokines TNF-alpha and IL-6 can be remarkably reduced, secretion of anti-inflammatory cytokines IL-10 can be improved, dose dependence is achieved, under the highest concentration, the influence on IL-6 secretion is 47.76 + / -1.02%, the influence on TNF-alpha secretion is 42.01 + / -4.64%, the influence on IL-10 secretion is 41.71 + / -1.67%, and good anti-inflammatory activity is achieved. In addition, the bioactive peptide has a remarkable proliferation effect on L929 cells, and it is indicated that the bioactive peptide has the potential of promoting skin wound healing.

The invention discloses a medical sodium hyaluronate skin repair gel and a preparation method thereof, and mainly relates to the technical field of hyaluronic acid, the medical sodium hyaluronate skin repair gel comprises the following components: sodium hyaluronate, a synergist, glycerol, carbomer, 1, 3-butanediol, triethanolamine and water. Compared with the prior art, the medical sodium hyaluronate skin repair gel prepared by the invention can relieve erythema, pruritus and other symptoms caused by skin inflammation; the wound dressing can also be used for nursing non-chronic wounds, such as superficial wounds, sutured wounds after operations, mechanical wounds and the like; meanwhile, a bacteriostatic environment for wound healing is kept, and skin repair is accelerated; the scar hyperplasia phenomenon in the skin wound repairing process is reduced.

The invention provides a superfine-diameter PCL / COL nanofiber membrane as well as a preparation method and application thereof, and relates to the technical field of skin wound repair materials. The PCL / COL nanofiber membrane material is prepared from PCL and I-type collagen as raw materials through electrostatic spinning and vacuum drying. According to the preparation method disclosed by the invention, an electrostatic spinning technology is combined with strict regulation and control on parameters, so that the stable and superfine 40-nanoscale PCL / COL nanofiber membrane material is prepared. The diameter of the superfine PCL / COL nanofiber membrane material reaches the bionic scale of the minimum natural collagen of an extracellular matrix, and meanwhile, the superfine PCL / COL nanofiber membrane material shows more excellent hydrophilicity and lower surface roughness, has good biological safety and mechanical stability, not only realizes high matching with a natural ECM hierarchical structure, but also has good application prospects. By enhancing the cell-material interface interaction, the adhesion, proliferation and migration capacities of fibroblasts are remarkably improved, the wound healing period can be effectively shortened, an excellent repair promoting effect is shown, and a solid foundation is laid for clinical transformation of materials.

The invention belongs to the technical field of regenerated medical dressings, particularly relates to a plant polysaccharide hydrogel medical dressing for promoting tissue damage repair and application thereof, and provides the following scheme that a medical and mechanical combined product comprises a regenerated medical dressing and a low-temperature sterile storage box for storing the regenerated medical dressing, the regenerated medical dressing comprises a hydrogel main body, open pores formed in the hydrogel main body, sustained-release vesicles arranged in the open pores, and stem cells and a cell culture medium which are filled in the sustained-release vesicles. The stem cell hydrogel can rapidly break blood, rapidly stop bleeding by being attached to a wound surface and promote wound closing, stem cells are inoculated to the upper surface of the hydrogel formed by dendrobium polysaccharides, aloe, lily, coptis chinensis and honeysuckle, safe application of the stem cells is achieved, active factors secreted by the stem cells are slowly released to the skin wound surface, and the wound healing effect is achieved. Through contact stimulation between cells, various cytokines can be secreted better, and the skin repair efficiency is improved.

The invention belongs to the technical field of bioengineering medicines, and particularly relates to a gel dressing for repairing skin wounds and a preparation method of the gel dressing. The anti-fouling coating is prepared from the following raw materials in parts by weight: 65 to 75 parts of polyvinyl alcohol, 14 to 20 parts of sodium alginate, 4 to 8 parts of sodium carboxymethyl cellulose, 1 to 3 parts of sodium hyaluronate, 12 to 22 parts of glycerol, 1 to 3 parts of surface anti-fouling additive, 6 to 10 parts of antibacterial microcapsule and 2 to 2.8 parts of compound ion. According to the invention, a PVA freeze-thaw physical network and an alginic acid Ca / Zn composite ion network cooperate to limit a pore channel, so that stable forming is realized; a chitosan / sodium tripolyphosphate microcapsule is prepared from polyhexamethylene biguanide hydrochloride and covered with sodium alginate, release sensitive to pH / ionic strength, acceleration in an inflammation stage and self-limiting in a later stage are constructed, and long-acting bacteriostasis is formed by SA-Zn site slow release; the stability and staged adaptation are highlighted through processes such as encapsulation, low-shear doping and the like and raw material synergy.

Disclosed herein are topical nitric oxide (NO) formulations containing a surfactant and methods that deliver NO to treat skin wounds, burns, infections, site of inflammations, or other skin conditions requiring treatment, as well as to eradicate and degrade biofilms on the skin surface. The NO formulations can be modified to prolong the time of NO exposure by modulating the surfactant concentration.

The invention discloses a ganoderma lucidum extract dressing. The ganoderma lucidum extract dressing comprises ganoderma lucidum source chitosan, ganoderma lucidum polysaccharide, ganoderma lucidum source carboxymethyl chitosan and carbomer. The invention further discloses application of the lucid ganoderma extract dressing in promoting skin wound repair and preparing products for promoting skin wound repair. The invention further discloses a preparation method of the lucid ganoderma extract dressing. The lucid ganoderma extract dressing disclosed by the invention has antibacterial and hemostatic functions, can effectively avoid the situation that common chitosan generates a sensitization effect, accelerates the wound healing and repairing process, and effectively improves the wound healing situation.

The invention relates to a skin wound repairing material and a preparation method thereof, and belongs to the technical field of biological medicines. The repair material disclosed by the invention contains palmitoyl tripeptide-1, methacrylated collagen, cuttlebone extract, recombinant human epidermal growth factor lipidosome, polydopamine nanoparticles, hyaluronic acid, sodium alginate modified silk fibroin and the like in a specific proportion. Wherein the cuttlebone extract is a product which is obtained by carrying out common enzymolysis on cuttlebone through chitinase and nattokinase and then carrying out step-by-step enzymolysis on the cuttlebone through gelatinase and lumbrukinase and is less than 5kDa. The components are prepared into an efficient product by adopting a special method, the stability of the components is improved, the problem that the activity is difficult to maintain is solved, and the skin wound repairing effect is good through reasonable compatibility.

The present invention discloses the preparation of an anti-inflammatory peptide derived from the hydrolysis of shell nacre protein and its application in skin repair. The peptide PDFDNGF provided by the invention can effectively promote the proliferation of RAW264.7 macrophage cells and inhibit the excessive production of nitric oxide and cytokines in RAW264.7 cells induced by lipopolysaccharide, while increasing the levels of anti-inflammatory cytokines, demonstrating significant anti-inflammatory activity. Furthermore, the active peptide has a significant proliferative effect on L929 cells, indicating its potential to promote skin wound healing. This invention provides a theoretical basis for the high-value utilization of shell nacre.

The present invention relates to the field of drug design technology, and specifically relates to an application of an extracellular polysaccharide of F. cyrtonema, which can be used to prepare a skin wound healing agent, especially a skin scald healing agent; specifically: the extracellular polysaccharide of F. cyrtonema can effectively inhibit the inflammatory response of the wound, promote the production of mouse cytokines, accelerate tissue repair and regeneration, and has a good effect on the healing of mouse scald skin. The present invention provides a new effective ingredient for the preparation of wound healing agents, and also provides a new direction for the application of the extracellular polysaccharide of F. cyrtonema.

The invention belongs to the technical field of biological materials, and particularly relates to a conductive hydrogel microneedle patch with antibacterial and anti-inflammatory activity and a preparation method of the conductive hydrogel microneedle patch. The preparation method comprises the following steps: firstly, loading plant essential oil with antibacterial and anti-inflammatory activity on mesoporous polypyrrole nanoparticles to prepare drug-loaded nanoparticles, then preparing a pre-hydrogel mixture containing the drug-loaded nanoparticles, and then filling the mixture into a PDMS microneedle mold to obtain the conductive hydrogel microneedle patch with antibacterial and anti-inflammatory activity. The prepared microneedle patch not only has excellent antibacterial and anti-inflammatory activity and a conductive function, but also has the characteristics of a microneedle and hydrogel, and can deliver a drug to a skin cuticle or a lower layer of a bacterial biofilm, so that the bioavailability of transdermal drug delivery of the drug is greatly improved. Besides, the microneedle patch is swelled after absorbing tissue exudate, is adhered to the surface of skin or other mucous membranes to continuously play antibacterial and anti-inflammatory roles and conductive functions, and provides wet healing conditions, so that high-quality healing of skin wounds is accelerated.

The invention discloses a bi-crosslinking gelatin-based hydrogel wound dressing as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method disclosed by the invention comprises the following steps: mixing a gelatin solution and an oxidized konjac glucomannan solution, heating and stirring to obtain a hydrogel precursor solution; and mixing the hydrogel precursor solution with a borax solution to obtain the bi-crosslinked gelatin-based hydrogel wound dressing. According to the method, oxidized konjac glucomannan and gelatin are subjected to Schiff base and Schiff base crosslinking, borax is further added to introduce a dynamic boric acid ester bond effect to enhance a three-dimensional network of the hydrogel, and the dynamic reversible Schiff base effect and the boric acid ester bond effect are enhanced, so that the pore diameter and the swelling ratio of the hydrogel are increased, and the gel strength of the hydrogel is enhanced; due to the good self-healing injectable performance, biodegradability and mechanical property of the hydrogel, the hydrogel can be used as a wound dressing for irregular deep skin wounds, and the technical problem that existing hydrogel is insufficient in treatment of irregular wounds is solved.

The invention belongs to the technical field of medical gel, and particularly relates to modified hydrogel for skin wound healing and a preparation method thereof.The modified hydrogel is prepared from, by weight, 2-5 parts of drug-loaded GDVLNs, 1-2 parts of chitosan, 0.6-1.5 parts of antioxidant, 0.2 part of LAP, 1.5 parts of MA, 18 parts of gel matrix and 100 parts of buffer solution, the drug-loaded GDVLNs prepared from the traditional Chinese medicine active ingredients and the ginseng vesicles have the functions of resisting oxidative stress, promoting angiogenesis, promoting cell proliferation and the like, and the MA modified hydrogel system can realize slow release and controlled release of the drug-loaded GDVLNs and play a long-acting healing promoting role.

The invention provides a nursing film device and system for hepatobiliary operation wound healing, and the nursing film device and system comprises a film-shaped carrier which is composed of a substrate layer and an adhesion layer, the substrate layer is adhered to the skin wound of a patient, and a plurality of hole grooves are formed in the substrate layer in a hollowed-out manner; the adhesion layer is adhered to the upper part of the substrate layer; the sensor assembly is composed of an infection indication sensor, an oxygenation sensor, a humidity indication sensor, a blood flow sensor and a wound healing progress sensor; monitoring the wound infection degree, oxygenation degree, humidity, blood flow degree and wound healing progress; the plurality of balloons are attached in the adhesive layer, and growth factors, analgesic and antibiotics are respectively wrapped in the balloons; wherein an electrode layer is arranged above the adhesion layer; the electrode layer is connected with a control assembly, and after the control assembly receives sensing information of the sensor assembly, the control assembly controls all the balloons to be released, and wound repairing is achieved.

The embodiment of the invention provides nano-enzyme hydrogel for promoting scar-free wound repair through inflammation regulation and c-Jun gene silencing and a preparation method of the nano-enzyme hydrogel. The nano-enzyme hydrogel is prepared from the following components in parts by mass: synthesized epsilon-polylysine modified CeO2 nano-enzyme particles and aldehyde hyaluronic acid, an instant Schiff base reaction between epsilon PL-CeO2 surface amino groups and HA-CHO molecule aldehyde groups is utilized, in-situ mixing is performed to form a hydrogel network, in addition, bFGF and siRNA are loaded into PLGA microspheres, and the hydrogel network is formed through in-situ mixing. And further embedding into the hydrogel to obtain the nano-enzyme hydrogel. The obtained nano-enzyme hydrogel has the functions of inflammation regulation, cell proliferation promotion, vascular regeneration promotion, c-Jun gene silencing and the like, and scar-free healing of skin wounds is remarkably promoted.

The invention discloses preparation of a shell nacre protein hydrolysis anti-inflammatory peptide and application of the shell nacre protein hydrolysis anti-inflammatory peptide in skin repair. The peptide PDFDNGF provided by the invention can effectively promote proliferation of macrophage RAW264.7 cells, can inhibit excessive production of nitric oxide and cytokines in the RAW264.7 cells induced by lipopolysaccharide, can increase the level of anti-inflammatory cytokines, and shows remarkable anti-inflammatory activity. In addition, the bioactive peptide has a remarkable proliferation effect on L929 cells, and it is indicated that the bioactive peptide has the potential of promoting skin wound healing. The method provides a theoretical basis for high-value utilization of the shell nacre layer.

The invention discloses a skin tension reducing device and method based on full-period adjustment, and belongs to the technical field of medical cosmetology. The skin tension reducing device comprises a left auxiliary pasting film, a right auxiliary pasting film, a plurality of tension adjusting devices and a tension detecting device. The adjusting device comprises fixed guide blocks, a traction belt and a conical spiral tightener; the two guide blocks are respectively fixed at the same height of the left auxiliary film and the right auxiliary film; the two guide blocks are respectively provided with a conical internal thread and a guide hole, and the conical spiral tightener is in threaded fit with the conical guide hole; one end of a traction belt is connected with a conical spiral tightener positioned on the left auxiliary film, and the other end of the traction belt is fixed on a fixed guide block positioned on the right auxiliary film through a guide hole; the tension detection device comprises strain gauges, a tension acquisition plate and a handheld display, and the strain gauges are attached to the traction belts and used for detecting the tension of the traction belts. Dynamic tension reduction management can be carried out on the whole cycle of skin wound healing, and the purpose of tension reduction is achieved in a high-quality mode.

Provided are a new polypeptide for repairing mucosal damage or a skin wound, and the use thereof. The polypeptide is not homologous with known polypeptides, can be stably present in vivo and in vitro, and has the effect of regulating stem cell proliferation and differentiation to repair mucosal damage or a skin wound. The present invention further relates to the use of the new polypeptide in the repairing of mucosal damage or a skin wound by means of regulating stem cell proliferation and differentiation, and the use thereof in the prevention, alleviation or treatment of gastrointestinal diseases.

The invention discloses a preparation method and application of a gynostemma pentaphylla exosome. According to the method, a high-activity callus is obtained by optimizing a callus induction culture medium, and a suspension culture system is established to realize biomass accumulation with the dry cell weight of 19.8 + / -0.7 g / L. A sequential progressive composite induction strategy (methyl jasmonate is added after chitosan is pre-induced for 24 hours) is innovatively adopted, so that the saponin content is remarkably increased to 102.6 + / -4.8 mg / g DW. Exosomes (P-Exos) are extracted from induced cells through an enzymolysis-magnetic bead affinity purification technology (WGA modified magnetic bead affinity capture after mild enzymolysis of a cracking buffer solution), the yield reaches 1.45 + / -0.08 mg / g cells, and the extraction efficiency is remarkably improved compared with that of a traditional gradient centrifugation method (C-Exos). Moreover, the gynostemma pentaphyllum exosome prepared by the invention can effectively improve the synthesis of skin collagen, has a better effect, can be applied to medicines for promoting skin wound repair or can be added into anti-aging cosmetics as a functional component, and has a very high application value.

The invention discloses composite hydrogel with a photo-thermal antibacterial effect and a preparation method of the composite hydrogel. The composite hydrogel is prepared from the following components: gold nanorods with a photo-thermal effect; silver nanoparticles grow on the surface of a gold nanorod through a reduction deposition method, and a silver-coated gold nanorod of a core-shell structure is formed; oxidized hyaluronic acid and carboxymethyl chitosan are subjected to Schiff base reaction and cross-linking to form a self-healing hydrogel matrix; the advantages of the Au-Ag-PEG nanoparticles and the hydrogel are combined, so that the Au-Ag-PEG hydrogel has an excellent antibacterial effect in vitro and in vivo; therefore, the nano composite hydrogel wound dressing has huge application potential in the aspect of treating infectious skin wounds.

The invention discloses stem cell freeze-dried powder for promoting skin healing and a preparation method of the stem cell freeze-dried powder. The freeze-dried powder comprises an effective component, a freeze-drying protective agent group and a polymer protective agent group, the effective component is umbilical cord mesenchymal stem cell conditioned medium freeze-dried supernatant, the protective agent group is selected from one or more of cane sugar, trehalose, lactose and mannitol, and the polymer protective agent group is selected from one or more of polyvinylpyrrolidone (PVP) and glucan. The preparation method comprises the following steps: collection of a conditioned culture medium, preparation of a freeze-drying protective agent solution, mixing treatment, low-temperature pre-freezing and freeze-drying, collection and packaging of freeze-dried powder and the like. The method can effectively retain stem cell-derived active factors and enhance the stability and redissolvability of the freeze-dried powder, and is suitable for skin wound repair, regenerative medicine and other scenes.

The invention provides a hydrogel dressing for synergistically promoting chronic wound healing through near-infrared controlled release of ROS (reactive oxygen species) and NO (nitric oxide) as well as a preparation method and application of the hydrogel dressing. Belongs to the technical field of biomedical materials. The hydrogel is prepared from the following components: upconversion nanoparticles as a core, silicon dioxide wrapping, carbon dot and L-arginine loading, maltotriose modification, and mixing and gelling with 2-amino-2-fluoro-2-deoxyadenosine as a hydrogel carrier, so as to obtain the hydrogel dressing. The obtained hydrogel dressing has the functions of inhibiting bacteria, promoting cell proliferation, promoting revascularization and the like, and remarkably promotes skin wound healing.