100 results about "Galacturonic acid" patented technology

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention provides application of pitaya peel polysaccharide in preparation of a medicine for repairing skin wounds. The monosaccharide components of the pitaya peel polysaccharide comprise rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. Research finds that the dragon fruit peel polysaccharide remarkably accelerates the wound healing process and promotes granulation tissue formation and collagen deposition. Meanwhile, the pitaya peel polysaccharide can also promote cell proliferation and neovascularization at the skin wound part, exert an anti-inflammatory effect, remodel skin microbiota and provide a more suitable microenvironment for skin wound healing. The invention provides a new drug choice for clinically repairing skin wounds, and can be used for developing new skin wound dressings.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

The invention discloses lotus leaf homogeneous polysaccharide LLP3-1 as well as a separation and purification method and application thereof, and belongs to the technical field of plant polysaccharide. The lotus leaf homogeneous polysaccharide LLP3-1 is composed of rhamnose, arabinose, galactose and galacturonic acid, the molar percentage of monosaccharide is 21.95: 25.33: 21.10: 31.62, the lotus leaf homogeneous polysaccharide LLP3-1 is acidic pectin polysaccharide with RG-I as a main structural domain and with part of side chains, the main chain is mainly composed of galacturonic acid and rhamnose, and the side chains are mainly composed of arabinose and galactose. The lotus leaf homogeneous polysaccharide LLP3-1 has the beneficial effects that the lotus leaf homogeneous polysaccharide LLP3-1 can obviously improve the symptoms of ulcerative colitis; the compound can be widely applied to preparation of medicines for treating ulcerative colitis, medicines for inhibiting proinflammatory factor secretion, antioxidation medicines, medicines for improving intestinal barrier integrity and functions, medicines for promoting short-chain fatty acid generation and medicines for improving intestinal flora disorder of ulcerative colitis.

This invention provides a Shoumei polysaccharide, its preparation method, and its application, belonging to the field of biomedical technology. The Shoumei polysaccharide is a homogeneous polysaccharide with the repeating sequence →4)-α-D-Gal. p A‑(1→, →4)‑β‑D‑Gal p ‑(1→,→4,6)‑β‑D‑Gal p ‑(1→,→6)‑β‑D‑Gal p -(1→、α-L-Ara f ‑(1→,→5)‑α‑L‑Ara f ‑(1→,→4)‑β‑D‑Glc p -(1→; The molecular weight of the Shoumei polysaccharide is 1100 kDa; The monosaccharide composition of the Shoumei polysaccharide is arabinose, galactose, glucose and galacturonic acid; The molar ratio of arabinose, galactose, glucose and galacturonic acid is 10.9:12.1:4.8:72.2, and the degree of galacturonic acid esterification is 33%. The Shoumei polysaccharide of the present invention can treat and / or prevent depression.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The embodiment of the invention discloses citrus peel pectin, citrus peel pectin emulsion and application. The content of the citrus peel pectin is 66-67%, the protein content is 13-14%, and the esterification degree is 72-73%; the sugar comprises the following components in percentage by mole: 58-59% of galacturonic acid, 25-26% of arabinose, 5-6% of galactose, 4-5% of rhamnose, 2-3% of glucose and 2-3% of xylose. The citrus peel pectin provided by the invention has excellent emulsibility; the citrus peel pectin emulsion has excellent emulsion stability, and shows a better encapsulation effect on LBE. The LBE emulsion obtained by encapsulating LBE by using CP has the characteristics of high encapsulation efficiency, low toxicity and high biocompatibility, and the synergistic effect of CP obviously enhances the antioxidant activity of LBE and the activity inhibition rate of tyrosinase, elastase and hyaluronidase, so that the LBE emulsion can be used as a novel anti-aging emulsion formula to be applied to cosmetics, and has a good application prospect. Good application prospects are realized.

The present invention aims to provide a novel pharmaceutical composition that can be used for the treatment or prevention of lysosomal storage diseases, particularly Niemann-Pick disease. [Solution] The present invention provides a pharmaceutical composition for the treatment or prevention of lysosomal storage diseases, characterized by containing a modified γ-cyclodextrin as an active ingredient, wherein the modified γ-cyclodextrin has a monovalent group derived from galacturonic acid that is bonded to a sugar residue of the γ-cyclodextrin via an amide bond.

The invention provides modified tartary buckwheat sprout acidic polysaccharide, a preparation method thereof and application of the modified tartary buckwheat sprout acidic polysaccharide in preparation of a medicine or health food with an immune enhancement function. The tartary buckwheat sprout acidic polysaccharide is rich in polygalacturonic acid (HG) and I-type polyrhamnogalacturonic acid (RG-I) pectin structural domains, and has remarkable in-vivo immune enhancement activity. The tartary buckwheat bud seedling acidic polysaccharide is subjected to structural targeting modification through a controllable partial acid hydrolysis technology and a directional enzyme digestion technology, and by comparing the in-vivo immune enhancement activity of the tartary buckwheat bud seedling acidic polysaccharide before and after targeting modification, it is found that the immune enhancement activity of the tartary buckwheat bud seedling modified acidic polysaccharide is remarkably superior to that of the tartary buckwheat bud seedling acidic polysaccharide.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses sequential slow-release temperature-sensitive hydrogel and a preparation method and application thereof.The preparation method comprises the following steps that a blood sample is subjected to programmed centrifugation, and a gel layer of leukocyte-rich platelet-rich fibrin L-PRF and a gel layer of improved platelet-rich fibrin A-PRF are obtained respectively; and the gel layer is cut into pieces, pre-frozen, freeze-dried, ground and sieved, so that L-PRF freeze-dried powder and A-PRF freeze-dried powder are obtained respectively, and the methacrylic acid gelatin microspheres entrapped with the L-PRF freeze-dried powder are prepared. The microspheres and A-PRF freeze-dried powder are loaded into a temperature-sensitive hydrogel system composed of chitosan, polygalacturonic acid and sodium beta-glycerophosphate, so that the growth factors can be effectively entrapped in the hydrogel structure, and continuous release is realized; the system has good mechanical strength and structural stability, the retention time of the material in vivo is prolonged, and stable three-dimensional network support is provided.

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.

The invention discloses solanum lyratum homogeneous polysaccharide as well as a preparation method and application thereof. The solanum lyratum homogeneous polysaccharide is mainly prepared from rhamnose, arabinose, galactose, glucose and galacturonic acid according to the molar ratio of 20.5: 20.6: 41.6: 2.2: 4.4, and the weight-average molecular weight of the solanum lyratum homogeneous polysaccharide is 7.0426 Kda. Soaking solanum lyratum thunb in water, decocting twice, combining filtrates, and carrying out rotary evaporation concentration; adding absolute ethyl alcohol into the concentrated solution while stirring, standing, and collecting bottom precipitate; redissolving the precipitate by using distilled water, and performing deproteinization treatment on a polysaccharide extracting solution obtained after redissolving by using a Sevag method to obtain a polysaccharide solution without an organic reagent; dialyzing the obtained polysaccharide solution by adopting a dialysis bag with the molecular weight of 3500Da to obtain a crude polysaccharide aqueous solution, and freeze-drying to obtain a solanum lyratum polysaccharide crude product; and separating and purifying the solanum lyratum polysaccharide crude product to obtain the solanum lyratum homogeneous polysaccharide. The solanum lyratum homogeneous polysaccharide prepared by the method has the advantages of fixed components, high yield, anticancer effect and good safety, and provides a basis for developing a potential new drug for colorectal cancer.

The invention belongs to the technical field of plant polysaccharide, and particularly discloses caulis spatholobi polysaccharide, a preparation method thereof and application of the caulis spatholobi polysaccharide in preparation of medicines for treating inflammation. Monosaccharide in the caulis spatholobi polysaccharide disclosed by the invention comprises mannose, glucuronic acid, galacturonic acid, glucose and arabinose in a molar ratio of (0.5-0.6): (0.3-0.5): (1-2): (1-2): (0.5-1.5). The caulis spatholobi polysaccharide provided by the invention is a novel uniform-structure polysaccharide which is separated from caulis spatholobi rattan for the first time, has a slightly acidic pH value, definite monosaccharide composition, definite molecular weight and clear glycosidic bond linking mode, has an anti-inflammatory effect, and is expected to be developed into a new anti-inflammatory drug. The preparation process is mild in condition, simple to operate and environment-friendly.

The application discloses a Salicornia polysaccharide as well as a preparation method and application thereof. The Salicornia polysaccharide is composed of 11 kinds of monosaccharides, wherein arabinose (24.96%), galactose (30.39%) and galacturonic acid (23.20%) are main monosaccharides of the polysaccharide, and the relative molecular weight is 3.24*10 4 Da. The Salicornia polysaccharide has a very special surface morphological structure, and presents a regular sawtooth distribution. The anti-tumor activity of the Salicornia polysaccharide is researched, and it is found that the polysaccharide can inhibit the growth of HepG2 cells, and the molecular mechanism of the anti-tumor activity is that the polysaccharide can induce the apoptosis of HepG2 cells. The Salicornia polysaccharide has significant anti-tumor activity, can be used as a candidate drug for developing an anti-tumor drug, and has a wide development prospect.

The invention belongs to the technical field of traditional Chinese medicine, and relates to polygonum cuspidatum homogeneous polysaccharide and a preparation method and application thereof.The polygonum cuspidatum homogeneous polysaccharide is mainly composed of galacturonic acid, rhamnose, galactose, glucuronic acid, glucose, mannose, xylose and glucuronide amine according to the molar ratio of 41.967: 24.624: 17.951: 5.427: 4.59: 3.501: 1.236: 0.358, and the weight-average molecular weight of the polygonum cuspidatum homogeneous polysaccharide is 26.57 kDa. The polygonum cuspidatum homogeneous polysaccharide is fixed in component, high in yield, good in hypoglycemic effect and good in safety, and a basis is provided for development of innovative hypoglycemic drugs.

The invention discloses a continuous graded extraction method of mulberry leaf flavonoids and mulberry leaf polysaccharides, which comprises the following steps of: firstly freezing mulberry leaves, then circulating high-pressure nitrogen, and finally circulating high-pressure steam, so as to efficiently extract the mulberry leaf flavonoids from the mulberry leaves and extract the mulberry leaf polysaccharides from residual mulberry leaf residues obtained by extracting the mulberry leaf flavonoids. According to the extraction method disclosed by the invention, the mulberry leaf residue waste is effectively utilized, the extraction rate of the mulberry leaf flavone is improved, the physicochemical properties of the mulberry leaf polysaccharide are improved and optimized, and particularly, the content of galacturonic acid in the mulberry leaf polysaccharide is remarkably improved, and the content of residual protein in the mulberry leaf polysaccharide is reduced, so that the obtained mulberry leaf polysaccharide is purified; the method is more suitable for constructing high-performance embedding, slow-release and sensing materials in the fields of biology, medicine and the like.

The present application belongs to the technical field of sugarcane leaf polysaccharide regulating intestinal flora. The present application provides a sugarcane leaf polysaccharide composition, which comprises mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose in a mass ratio of 6.8:1.5:5.2:40.7:13.1:18.9:1.6:6.1:1.9. The composition of the present application can improve the imbalance of intestinal flora caused by obesity and hyperglycemia by reducing the ratio of Firmicutes / Bacteroidetes and increasing the abundance of probiotics.

The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof, in particular to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof. The purpose of the invention is to determine one component in the nine-steamed nine-processed polygonatum sibiricum product. NPCP-A1 is extracted by a water extraction and alcohol precipitation method, deproteinized by a Sevag method, and subjected to chromatographic separation and purification by a DEAE-52 anion exchange column and a Sephacryl S-300 gel column, and the NPCP-A1 is prepared from arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The proliferation of RAW264.7 cells and the secretion of NO, TNF-alpha and IL-6 can be promoted. The composition is used for preparing health-care products or medicines for improving immunity. According to the acidic polysaccharide prepared by the method disclosed by the invention, the polysaccharide plays an immune regulation role by promoting macrophage proliferation and increasing secretion of cell factors such as NO, TNF-alpha, IL-6 and the like.

The present disclosure relates to methods and compositions for quantifying the relative distribution of glucuronidation, iduronidation, and / or galacturonidation of polypeptides, including stereoselective liquid chromatography mass spectrometry (LC-MS) methods that can achieve simultaneous separation and relative quantification of glucuronidation, iduronidation, and / or galacturonidation of polypeptides.

The present application provides a pectin gel and a preparation method therefor. The pectin gel comprises pectin and a fat-soluble substance. The pectin comprises pectin. The weight-average molecular weight of the pectin is in the range of 5.0*10^4 Da to 6.0*10^5 Da, and the polydispersity index is 1 to 6. In the structural domains of the pectin, HG > 30 mol%, and RG-1 ≤ 35 mol%; HG is homogalacturonan, and RG-1 is rhamnogalacturonan type I. The emulsion stability of the pectin gel prior to molding is high. Thus, the molded pectin gel has good textural stability, low water syneresis and oil exudation, and a good mouthfeel.

The present application relates to the technical field of multilayer liposome, and particularly relates to a polysaccharide double-layer modified liposome and preparation and application thereof. The polysaccharide double-layer modified liposome takes the oleanolic acid (OA) liposome as a core, and is coated with chitosan (CHI) and polygalacturonic acid (PGA) two kinds of polysaccharides on the surface in sequence to form a double-layer modified oleanolic acid liposome (PGA-CHI-OA liposome) with a core-shell structure. The modifiers chitosan and polygalacturonic acid used are food raw materials, and are low in cost and rich in resources. Under certain conditions, the two can be self-assembled on the surface of the liposome by mutual attraction of positive and negative charges, the preparation method is simple, the PGA-CHI-OA liposome particles formed are uniform in dispersion, and the PGA-CHI-OA liposome particles are high in safety, stability and bioavailability, and can be applied to food and health products.

The application provides a self-crosslinking sugar beet pectin and a preparation method and application thereof, and relates to the technical field of food and cosmetics. The preparation method comprises the following steps: mixing sugar beet meal with water and performing heating extraction to obtain an extraction liquid; mixing the extraction liquid with anhydrous ethanol and performing alcohol precipitation, and washing the obtained precipitate to obtain a wet pectin precipitate; performing heat treatment on the wet pectin precipitate to obtain heat-modified pectin; and drying the heat-modified pectin to obtain the self-crosslinking sugar beet pectin. The application can improve the chemical crosslinking between the carbohydrate components and the protein components of the sugar beet pectin (i.e. improve the degree of self-crosslinking) while improving the galacturonic acid content of the sugar beet pectin, thereby significantly improving the emulsifying properties of the sugar beet pectin. The application well balances the relationship between the high galacturonic acid content and the improvement of the emulsifying properties of the sugar beet pectin, and finally obtains a sugar beet pectin product with high galacturonic acid content and good emulsifying properties.

The invention discloses a stringy stonecrop herb polysaccharide extraction process which comprises the following steps: drying and crushing stringy stonecrop herb, adding water according to a material-liquid ratio of (1: 20)-(1: 60), extracting, concentrating, and carrying out alcohol precipitation to obtain crude polysaccharide; deproteinizing, dialyzing and freeze-drying; carrying out gradient elution and purification to obtain sedum sarmentosum polysaccharide SSBP; an extraction process is optimized through a response surface method, optimal conditions are selected, and the yield of crude polysaccharide reaches 19.06%; according to structural characterization, SSBP is acidic polysaccharide, the molecular weight is 7.484 kDa, 36.12% of uronic acid is contained, monosaccharide is composed of rhamnose, arabinose, galactose, galacturonic acid and glucuronic acid, and the molar ratio of rhamnose to arabinose to galactose to galacturonic acid to glucuronic acid is 12.85: 19.13: 18.98: 35.73: 3.35; an in-vitro activity experiment shows that the SSBP has good antioxidant activity and remarkable anti-inflammatory activity, the release of RAW264.7 macrophage NO induced by LPS and the secretion of IL-6, IL-1beta and TNF-alpha can be inhibited in a dose-dependent manner, and the inhibition rates under the concentration of 1.2 mg / mL respectively reach 74.41%, 92.53%, 95.27% and 95.73%; and a new scheme is provided for deep development of sedum sarmentosum polysaccharide and preparation of anti-inflammatory and anti-oxidation products.

The application discloses a rehmannia polysaccharide, a preparation method thereof and application of the rehmannia polysaccharide in preparation of a medicine for preventing and treating kidney injury caused by cisplatin. The rehmannia polysaccharide contains the following monosaccharides in percentage by mass: rhamnose (Rha) 10.265%-14.963%, glucuronic acid (GlcA) 0.514%-0.904%, galacturonic acid (GalA) 35.784%-40.596%, glucose (Glc) 3.128%-5.048%, galactose (Gal) 37.892%-41.438% and arabinose (Ara) 3.869%-4.865%. The structure of the rehmannia polysaccharide is clear, and the rehmannia polysaccharide shows a significant improvement effect on cisplatin nephrotoxicity through cell experiments and animal experiments, and has important value for developing a new and effective prevention and treatment product of cisplatin-induced acute kidney injury.

The application discloses a method for improving the drainage of cotton stalk chemi-mechanical pulp, and belongs to the field of pulping and papermaking. The method comprises the following steps: after the cotton stalk is crushed, washed and steamed, a first chemical impregnation agent is added, and then mechanical disintegration is performed to obtain cotton stalk material; pectinase is used to perform enzyme pretreatment on the cotton stalk material to obtain pretreated material; a second chemical impregnation agent is added to the pretreated material, mechanical disintegration is performed, and then refining disintegration is performed to obtain refined material; after the refined material is washed, enzyme treatment is performed on the refined material by using pectinase to obtain cotton stalk chemi-mechanical pulp with obviously improved drainage. By selecting pectinase as a biological enzyme and designing a two-stage treatment process of pectinase, pectin substances in cotton stalk raw materials can be effectively removed, pectin is degraded into small molecular substances such as galacturonic acid, the drainage of cotton stalk chemi-mechanical pulp is improved, and the influence of pectin on the papermaking process is eliminated.

The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 μL of NaOH-DMSO suspension (1 mg / mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 μL of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

The invention discloses a preparation method of fructus alpiniae oxyphyllae pectic polysaccharide and application of the fructus alpiniae oxyphyllae pectic polysaccharide in treatment of Alzheimer's disease. The pectic polysaccharide is prepared by taking fructus alpiniae oxyphyllae as a raw material through the steps of degreasing, water extraction, alcohol precipitation, deproteinization, dialysis, chromatographic purification and the like, and is a uniform acidic polysaccharide. Structural characterization results show that the pectic polysaccharide is mainly composed of rhamnose, glucuronic acid, galacturonic acid and galactose, the content of galacturonic acid is dominant, part of residues are subjected to O-methylation and / or O-acetylation modification, and the pectic polysaccharide has a pectic acidic polysaccharide structure with alpha-(1-> 4)-D-galacturonic acid as a main chain and branched chains. Animal experiment results show that the fructus alpiniae oxyphyllae pectic polysaccharide can significantly improve the learning and memory ability and cognitive impairment of Alzheimer disease model animals. The fructus alpiniae oxyphyllae pectic polysaccharide provided by the invention is clear in source and stable in preparation method, can be used for preparing a pharmaceutical composition or functional food for preventing and treating the Alzheimer disease, and has a good application prospect.

The invention discloses application of uncaria neutral polysaccharide URP-W in preparation of anti-glioma drugs, and belongs to the technical field of antitumor of medicinal plant polysaccharide. The preparation method comprises the following steps: obtaining a crude polysaccharide extract from uncaria through a hot water extraction and ethanol precipitation method, and purifying the crude polysaccharide extract through a DEAE cellulose column and sephadex G200 to obtain the uncaria neutral polysaccharide URP-W; the monosaccharide of the URP-W comprises arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and mannuronic acid, and the molar ratio of the arabinose to the rhamnose to the galactose to the glucose to the xylose to the mannose to the galacturonic acid to the mannuronic acid is 17.99: 6.32: 21.71: 27.17: 2.56: 7.57: 16.20: 0.49. Experiments find that the URP-W can inhibit proliferation of glioma and cause apoptosis, has remarkable anti-tumor activity on glioma cells, and provides a new medicine raw material and a new treatment method for clinical treatment of glioma.

The invention belongs to the technical field of biological medicines, and particularly relates to a white hyacinth bean flower homogeneous polysaccharide as well as a preparation method and application thereof. The purified white hyacinth bean flower polysaccharide (BDHPE2-F) is separated and purified from the white hyacinth bean flower crude polysaccharide, and the obtained BDHPE2-F is an acidic polysaccharide which mainly contains galacturonic acid and galactose, has the molecular weight of 10.814 kDa and has very high methyl esterification degree. As a novel polysaccharide, the BDHPE2-F is low in price and wide in application, has high anti-inflammatory activity and has development and commercial value.

The invention relates to Leclercia adcarboxsaca and an application thereof in degradation of corn straws, the Leclercia adcarboxsaca is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 32394. The strain can secrete AA2 family DyP-type peroxidase, GH1 / GH3 beta-glucosidase, PL8 oligogalacturonic acid lyase and GH88 unsaturated glucuronic acid hydrolase, and has the capability of synergistically degrading lignocellulose. The culture method comprises the steps of activation, seed solution preparation and fermentation. The obtained fermentation liquor is applied to the corn straw in proportion, so that the surface structure of the straw can be effectively destroyed, and the degradation rate is increased; meanwhile, soil nutrients are remarkably activated, the content of rapidly available phosphorus, rapidly available potassium, soluble organic carbon, alkali-hydrolyzable nitrogen and the like in the soil is greatly increased, and the synergistic interaction of efficient straw degradation and soil fertility improvement is achieved.