316 results about "Galacturonic acid" patented technology

Processes for producing ethanol from citrus waste by reducing the concentration of limonene in citrus waste to allow fermentation are disclosed. In one embodiment a slurry of ground citrus waste 1 is partially hydrolyzed by heating using a jet cooker 2 and then injected into a flash tank 4 to remove limonene 5. The heated citrus waste is then cooled, hydrolyzed with enzymes and fermented to ethanol. An alternative method of limonene removal uses enzymatic hydrolysis followed by centrifugation 27 to separate sugar-containing liquid from residual citrus waste solids containing limonene. Sugars are fermented and ethanol is distilled from the fermented mixture/beer. The remaining solids and liquids may be processed further to yield other byproducts. More particularly, the solids may be dried and pressed for use in cattle feed and the liquids may be further fermented or processed to yield additional ethanol, acetate, galacturonic acid monomers and polymers, five carbon sugars and other products.

The invention relates to a colloid bismuth pectin compound and a quality control method of pharmaceutical compositions of the colloid bismuth pectin compound, and specifically provides a colloid bismuth pectin compound, new quality control indexes of pharmaceutical compositions of the colloid bismuth pectin compound and a detection method of the colloid bismuth pectin compound and the pharmaceutical compositions of the colloid bismuth. In the prior art, the quality of products having poor efficacy can not be well controlled because the efficacy-related indexes are not controlled strictly in the existing quality control methods of colloid bismuth pectin and pharmaceuticals thereof. Based on the existing quality control methods of colloid bismuth pectin and pharmaceuticals thereof, the following detection items and indexes are added: intrinsic viscosity, gel property, uniformity and galacturonic acid content. The invention can effectively ensure clinical efficacy of products, make the product quality standards more scientific, reasonable and controllable, and has great significance in quality control of the colloid bismuth pectin and the pharmaceutical preparation of colloid bismuth pectin.

The invention discloses a Ziyang selenium-rich green tea selenium-containing polysaccharide, which comprises the following components in percentage by mass: 1.5-8.5 ppm selenium and the balance of heterosaccharide. The heterosaccharide comprises the following components in percentage by mol: 3.3-5.3% of mannitose, 1-2% of ribose, 3.5-5% of rhamnose, 2-3.5% of glucuronic acid, 2.6-3.8% of galacturonic acid, 30-34.2% of glucose, 3.6-5.6% of xylose, 19.8-23.2% of galactose and 21.5-25.6% of arabinose. The invention also provides a preparation method and application of the Ziyang selenium-rich green tea selenium-containing polysaccharide. The preparation method is simple and convenient, has the advantage of low cost, and is suitable for popularization; and the selenium-containing polysaccharide has the action of enhancing immunity, has obvious inhibiting actions on growth of colon cancer cells, and has obvious protective actions on hepatic injury.

The invention relates to a new application of low molecular citrus pectin, in particular to the application of low molecular citrus pectin for treating and preventing lead and heavy metal poisoning in prepared medicines, foods and health care products. The low molecular citrus pectin is a compound prepared from soluble dietary fiber and galacturonic acid, the polysaccharide derivative is used as a metal ion absorbent (chelating agent), has absorption action on heavy metal ions, can remove heavy ions, such as Zn2+, Cu2+, Cd2+, Ag+, Cr3+, Ni2+, Hg2+, Pb2+ and the like. The low molecular citrus pectin is different from common chemical chelating agents; proved by animal and clinic experiments, lead, mercury and arsenic are discharged out of human bodies during toxin discharge, and the key level of calcium, magnesium, zinc or other minerals can not be influenced. The natural chelating function of the low molecular citrus pectin is a most ideal and successful solution for heavy metal pollution in the human body at present. Thus, the low molecular citrus pectin has the function of preventing various diseases and especially has special functions of clearing away harmful cells and discharging toxins in the human body.

The invention discloses a method for improving yield of salvianolic acid B in savia miltiorrhiza suspension culture cells by inducing, specifically comprising the following steps: 1) cultivating the aseptic seedlings; 2) inducing the callus; 3) sub-cultivating the callus; 4) cultivating the callus by suspending; 5) inducing to produce the salvianolic acid B; and 6) harvesting the culture cells. The method solves the problem that the salvianolic acid B is not produced after the inducing factors, such as yeast extract, galacturonic acid, mycelia extract and the like are added. The content of the salvianolic acid B in the savia miltiorrhiza suspension culture cells is increased by about 5 times by using the method. The technology condition provided by the invention is suitable for industrial production of the salvianolic acid B on a large scale by a cell culture method after being expanded.

Method for producing ethanol from solid citrus waste by reducing the concentration of limonene in citrus waste to allow fermentation. In one embodiment ground solid citrus waste is partially hydrolyzed and pasteurized by heating using a jet cooker and then injected into a flash tank to remove limonene. The heated citrus waste is then cooled, hydrolyzed with enzymes and fermented to ethanol. The remaining solids and liquids may be processed further to yield other byproducts. More particularly, the solids may be dried and pressed for use in cattle feed and the liquids may be further fermented or processed to yield additional ethanol, acetate, galacturonic acid monomers and polymers, five carbon sugars and other products.

The invention relates to glucose-rich spirulina polysaccharide and an application thereof in a plant green pesticide and an anti-tumor drug. High-sugar spirulina obtained through nutritional regulation is used as a raw material and is subjected to hot water extraction, protein removal with a flocculant, concentration, precipitation and freeze drying to obtain the water-soluble high-sugar spirulina polysaccharide having the molecular weight of 10-200 kDa; the spirulina polysaccharide comprises the monosaccharides mainly comprising rhamnose, fucose, arabinose, xylose, mannose, galactose, glucose, glucuronic acid and galacturonic acid, and is characterized in that the glucose content is more than 50% in the monosaccharide composition. The spirulina polysaccharide can obviously induce plant disease resistance, has obvious anti-tumor effect on tumor, and can be used as the biological pesticide and the anti-tumor drug.

A method of preventing or treating infection by a range of fungal pathogens in an animal or plant or stored plant product comprising administering an effective amount of a sugar acid, in particular mannonic acid, gluconic acid or galacturonic. The sugar acid can be delivered by a biocontrol that can manufacture the sugar acid. An exemplified biocontrol is Pseudomonas strain AN5, which is characterized by the capacity to convert aldose to sugar acid. Also disclosed are nucleotide sequences from enzymes in the Pseudomonas PQQ enzyme pathway which may be used to transform microorganisms and plants so that they produce sugar acids at levels sufficient to control the growth or viability of pathogens.

Disclosed is a method for improving tobacco extractive incense by multiple biological enzymes, which comprises charging pectolase, cellulose, hemicellulase and beta-glucosidase for enzymolysis, thus producing many kinds of scent generation constituents originally non-existent in the tobacco extract, as well as a plurality of micromolecular carbohydrates and beta-galacturonic acid.

The invention discloses an enzymatic preparation method of low molecular pectin. The method comprises the following steps: carrying out enzymolysis and de-esterification on high molecular high ester pectin by virtue of one or more of pectinase, cellulase, galacturonase and pectinesterase; and carrying out alkali liquor neutralization, filtration and drying and grinding on the product to obtain a pectin product with targeted molecular weight and degree of esterification. According to the method disclosed by the invention, the high molecular high ester pectin is hydrolyzed by adopting a multienzyme compound hydrolyzing technology so as to extract pectin with different molecular weights and degrees of esterification, high content of galacturonic acid and excellent taste from natural pectin. The low molecular pectin powder prepared by the preparation method meets the USP30 standard, various chance elements in the prior art are avoided, and the purity and success rate for extracting the low molecular pectin are improved. Meanwhile, the method is short in process flow, strong in capability of industrialization and suitable for being popularized on a large scale.

The invention relates to a phosphated Arillus Longan polysaccharide which is composed of glucose, arabinose, galacturonic acid, levulose and sorbose. The molecular weight of the phosphated Arillus Longan polysaccharide is 112518.5. The synthesis method of the phosphated Arillus Longan polysaccharide comprises the following steps: (1) separating and extracting Arillus Longan polysaccharide; (2) removing proteins and pigments; (3) carrying out graded purification on the Arillus Longan polysaccharide by a diethylaminoethyl cellulose column; (4) purifying the Arillus Longan polysaccharide by a Sephacryl S-300 HR column; and (5) esterifying the Arillus Longan polysaccharide with a phosphating reagent POCl3. According to the invention, an artificial synthesis method can be used for preparing the phosphated Arillus Longan polysaccharide which contains glucose, arabinose, galacturonic acid, levulose and sorbose.

The invention discloses codonopsis pilosula uniform polysaccharide CPPib which is mainly composed of, by Mole percentage, 44.28% of rhamnose, 20.42% of galactose, 11.32% of arabinose and 23.97% of galacturonic acid, weight-average molecular weight of the codonopsis pilosula uniform polysaccharide CPPib is 1.45*105Da, and main carbohydrate chains are 1,2 connected rhamnose (rha, 45%), 1,4-connected galacturonic acid (GalA, 25%) and 1,2,6 connected galactose (Gal, 30%). A preparation method of the codonopsis pilosula uniform polysaccharide CPPib has the advantages of being simple, scientific, reasonable, and capable of preparing products with very high purity with only need of passing through an anion exchanging column chromatograph once. The prepared products have the advantages of having anti-tumor effect, educational effect, very high clinical application value and healthcare function.

The invention discloses a gentian acidity uniform polysaccharide and a purification method and application thereof and belongs to the field of gentian acidity uniform polysaccharide purification. The gentian acidity uniform polysaccharide comprises the following components monosaccharide including, by mole percentage content, 1-1.5% of fucose, 11-12% of rhamnose, 22-23% of arabinose, 17-18% of galactose, 3-3.5% of glucose, 3-3.5% of xylose, 0.5-1% of mannose and 39-40% of galacturonic acid. According to the gentian acidity uniform polysaccharide and the purification method and the application thereof, further separation and purification of the gentian polysaccharide are achieved, the gentian acidity uniform polysaccharide with the weight-average molecular weight of 5.8*104 Da is obtained, the anticoagulant activity of the gentian acidity uniform polysaccharide is high, the preparation method is simple, the hidden danger existing in the residual chemical reagent is avoided, and the gentian acidity uniform polysaccharide has good development application prospect.

The ginseng extracting process capable of separating ginseng polyse from ginseng extract includes the following steps:1. extracting ginseng with 50-85 % concentration alcohol solution to obtain filtrate and filter residue; 2. vacuum drying filtrate to obtain ginseng extract; and 3. extracting the filter residue with water and spraying the water extract to obtain ginseng polyse. The ginseng extracting process has alcohol extraction, water extraction and alcohol precipitation combined organically to produce light brown polyse with effective component galacturonic acid content of 13-30 %, 10 % higher that of traditional process, and best utilization of the side product to obtain ginseng extract.

The invention discloses a method for extracting low-methoxy pectin from apple pomace, comprises the following steps: washing apple pomace by using water at 30-40 DEG.C, microwave treating at 380-500 W and then filtering, microwave drying, the filtered apple pomace, stoving and crushing to obtain apple pomace powder, mixing the apple pomac powder and acid water to perform microwave treatment at 480-500 W, concentrating centrifuged supernatant as concentrated solution, pouring the concentrated solution in an upright filled with active carbon, and collecting the filtrate; heating the filtrate to 5-10 DEG.C, adding alkali solution to regulate the pH value, adding the acid water in the obtained pectin concentration solution to regulate the pH value to 3-5, adding ethanol water in the pectin concentrated solution, mixing, standing and centrifuging to obtain the precipitate, washing by using the ethanol water for 7-8 times, washing, precipitating, vacuum freezing and drying to obtain a substance, namely, the low-methoxy pectin. The process is coherent and simple, the required reagent is reagent in common use, low in cost and easy to purchase; the extracting rate can achieve 7.9-9.4%, the esterfication degree is 38.7-41.4%, and the galacturonic acid content is 72-82%.

Sperm, oocyte, and embryo survival and function is improved in vivo or in vitro by the use of a polysaccharide containing arabinose, galactose and/or hexuronic acid. In particular, a nonspermicidal lubricant containing such a polysaccharide (e.g., gum arabic, pectin, or galacturonic acid) increases the fertilization potential of the sperm during coitus, artificial insemination or sperm collection. Similarly, a freezing medium containing a polysaccharide containing arabinose, galactose and/or hexuronic acid enhances sperm, oocyte, or embryo viability.

The invention discloses a diaphragma juglandis acidic polysaccharose and a preparation and an application thereof. The diaphragma juglandis acidic polysaccharose is prepared from polysaccharide with the weight percent being 99% or above, wherein the polysaccharide comprises galacturonic acid, glucose, galactose and arabinose at the mass ratio of (66.30-72.50) to (13.50-18.60) to (6.20-11.40) to (2.80-4.40); the preparation method of the diaphragma juglandis acidic polysaccharose comprises the following steps: extracting diaphragma juglandis powder crude polysaccharide by water extraction and alcohol precipitation, removing protein by employing an enzyme-Sevage combined technique, dialyzing and purifying by anion exchange chromatography and gel filtration chromatography; and carrying out vacuum freeze-drying, separating and purifying to obtain the diaphragma juglandis acidic polysaccharose. The purified polysaccharide is subjected to component analysis, structural identification and immune function research, so that the diaphragma juglandis acidic polysaccharose has relatively high anti-oxidation activity, can be used as an antioxidant or applied to preparation of the antioxidant, and can be widely applied to cosmetics, food supplements, animal feed additives, medicines and the like.

The invention relates to the technical field of medicines, and discloses glabrous sarcandra herb acidic polysaccharide quality product SGP-2. The glabrous sarcandra herb acidic polysaccharide is characterized in that the content of polysaccharide is over 90 percent, and the number-average molar mass is 1,000-2,000KDa; monosaccharide comprises glucose, galactose, mannose, arabinose and galacturonic acid; the bond types of glucosidic bond comprise alpha(1->4)galacturonic acid, alpha(1->4) galacturonic acid methyl ester, alpha(1->4) arabinose, alpha(1->4)mannose, beta(1->6) glucose, beta(1->3)galactose, alpha(1->4)glucose, beta (1->4,6)glucose, alpha(1->3,6)galactose, and alpha(1->4,6)galacturonic acid. The invention provides application of the glabrous sarcandra herb acidic polysaccharide quality product SGP-2 in inhibiting alpha-glucosidase activity and application of glabrous sarcandra herb acidic polysaccharide in preparing a medicament for treating diabetes mellitus, and provides application of the glabrous sarcandra herb acidic polysaccharide quality product SGP-2 in preparing anti-tumor medicaments.