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Glabrous sarcandra herb acidic polysaccharide, preparation method and application thereof

A technology of acidic polysaccharides and sarcomas, which is applied in the direction of antineoplastic drugs, metabolic diseases, drug combinations, etc., can solve the problems of less research on macromolecular substances, and achieve good social and economic benefits, broad application prospects, and impurities little effect

Active Publication Date: 2014-02-05
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the researches on sarcomas are concentrated on small molecule chemical components such as sesquiterpenes, coumarins, flavonoids, and saponins, but there are few related studies on macromolecular substances such as polysaccharides

Method used

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  • Glabrous sarcandra herb acidic polysaccharide, preparation method and application thereof
  • Glabrous sarcandra herb acidic polysaccharide, preparation method and application thereof
  • Glabrous sarcandra herb acidic polysaccharide, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The specific preparation method of the sarcomas acidic polysaccharide fine SGP-2 of the present invention is as follows:

[0044] (1) Weigh 100 g of the whole herbal material of S. chinensis that has been crushed and passed through an 80-mesh sieve into an extraction container, add 5 times the volume of ethanol, reflux and degrease for 3 hours, remove the supernatant with 4 layers of gauze, and make up the residue with deionized water to 1000 mL. Extract in a water bath at 70°C for 1 hour with intermittent stirring. After collecting the extract, the residue was supplemented with deionized water to 1000 mL, extracted in a water bath at 70°C for 1 hour, and stirred intermittently. Filter the residue with gauze, concentrate the combined extracts to 20mL, centrifuge at 4000r.p.m / min to remove the precipitate, add 1 times ethanol to precipitate, place at 4°C for 24 hours, centrifuge to remove the supernatant, wash with absolute ethanol for 3 times, and dry in vacuo to obtain...

Embodiment 2

[0058] Using 4-nitrophenyl-α-D-glucopyranoside (PNPG) as a substrate, the α-glucosidase inhibitory activity of SGP-2, a refined polysaccharide from S. sargassum obtained in Example 1, was evaluated. Take 60 μL of Sarcodon sarcoides acidic polysaccharide boutique SGP-2160 μL of different concentrations and mix them with 20 μL (1 U / mL) of α-glucosidase solution, incubate at 37 °C for 10 min, then add 20 μL (5 μmol / L) of PNPG, and incubate at 37 °C for 30 min. Acarbose was used as a positive control. Calculate the inhibitory rate of SGP-2 to α-glucosidase. As shown in Table 3, SGP-2, a refined polysaccharide from Sarcodon sarcoides, can effectively inhibit the activity of α-glucosidase in vitro, and the inhibition rate increases with the increase of the concentration, and when the concentration of SGP-2 is 125 μg / mL Equivalent to the inhibition level of acarbose 500μg / mL.

[0059] Table 3 Inhibition of α-glucosidase in vitro by the acidic polysaccharide SGP-2 from Sargassum

...

Embodiment 3

[0063]The hypoglycemic effect of SGP-2, the refined acidic polysaccharide from S. chinensis obtained in Example 1, was detected on a high-fat combined STZ-induced diabetic mouse model. Select male C57 / BJ mice with a body weight of 18-22 g, and after feeding with high-fat diet for 3 weeks, inject streptozotocin (STZ) intraperitoneally at a dose of 100 mg / kg, and continue to feed with high-fat diet for 3 weeks to monitor the mice. rat blood sugar. Select the mice whose postprandial blood glucose value is greater than 11.1mmol / L, and randomly divide them into 5 groups, 8 mice in each group, set the model group: intragastric administration of normal saline; positive drug group: intragastric administration of 10mg / kg acarbose; Low-dose group of SGP-2 high-quality acidic polysaccharide SGP-2: 150 mg / kg SGP-2 by intragastric administration; medium-dose SGP-2 group: 300 mg / kg SGP-2 by intragastric administration SGP-2 high-dose SGP-2 polysaccharide; SGP-2 high-dose group: 600 mg / kg S...

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Abstract

The invention relates to the technical field of medicines, and discloses glabrous sarcandra herb acidic polysaccharide quality product SGP-2. The glabrous sarcandra herb acidic polysaccharide is characterized in that the content of polysaccharide is over 90 percent, and the number-average molar mass is 1,000-2,000KDa; monosaccharide comprises glucose, galactose, mannose, arabinose and galacturonic acid; the bond types of glucosidic bond comprise alpha(1->4)galacturonic acid, alpha(1->4) galacturonic acid methyl ester, alpha(1->4) arabinose, alpha(1->4)mannose, beta(1->6) glucose, beta(1->3)galactose, alpha(1->4)glucose, beta (1->4,6)glucose, alpha(1->3,6)galactose, and alpha(1->4,6)galacturonic acid. The invention provides application of the glabrous sarcandra herb acidic polysaccharide quality product SGP-2 in inhibiting alpha-glucosidase activity and application of glabrous sarcandra herb acidic polysaccharide in preparing a medicament for treating diabetes mellitus, and provides application of the glabrous sarcandra herb acidic polysaccharide quality product SGP-2 in preparing anti-tumor medicaments.

Description

technical field [0001] The present invention relates to herbal medicine Sargassum sargassum, specifically Sargassum sargassum acidic polysaccharide essence SGP-2, and relates to the application of Sargassum sargassum acidic polysaccharide essence SGP-2 in inhibiting the activity of α-glucosidase and in the preparation of medicines for treating diabetes The present invention also provides the application of SGP-2 in the preparation of antitumor drugs. Background technique [0002] Diabetes mellitus is a very common endocrine and metabolic disorder, and it is a chronic disease characterized by hyperglycemia caused by insulin secretion and utilization disorders. The incidence of diabetes is increasing year by year and has become a prominent problem all over the world. One of the effective ways to prevent and treat such diseases is to limit or delay the breakdown of carbohydrates in food in the digestive tract. The human body's utilization and absorption of carbohydrates such ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P3/10A61P35/00
Inventor 高向东张珍珍刘玮郑颖姚文兵
Owner CHINA PHARM UNIV
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