The invention relates to corn stigma oral liquid with an anti-fatigue function, which is prepared by taking corn stigma, chrysanthemum and liquorice as raw materials, and subjecting the materials to extracting, filtering, mixing, sterilizing and filling. The corn stigma anti-fatigue oral liquid is administrated to mice in a high-
dose group, a mediate-
dose group, a low-
dose group and a blank control group through gavage for 30 d, and the results show that by administrating the corn stigma oral liquid, the burden swimming time of the mice can be prolonged; the activity of
lactic acid dehydrogenase is increased; the generation of
urea nitrogen is reduced; and
hepatic glycogen of the mice in different groups is improved to different extent. An
acute toxicity test shows that LD 50 can not be detected, suggesting that the corn stigma oral liquid has no toxic or
side effect. In a maximum dosing test, the mice are administrated with the corn stigma oral liquid in high, mediate and low doses three times a day, and the blank group is administrated with equal volume of
saline through gavage. The mice are observed for 14 d after dosing, death does not occur, piloerection, moving restlessly,
tail erecting, excited
jumping,
curling up, hyperactivity,
slow response to stimulation or
allergy does not occur, and symptoms such as stiffness, fremitus,
ataxia do not occur either, suggesting that the
maximum dose of the corn stigma oral liquid for the mice is 60 g / kg.