41 results about "Dose group" patented technology

The invention relates to a moldavica dragonhead extract, a moldavica dragonhead dropping pill and a production method thereof, wherein, the moldavica dragonhead extract contains higher total flavonoids and luteolin and the production method of the moldavica dragonhead extract is simply operated. Pharmacodynamics test result indicates that the moldavica dragonhead dropping pill acquired from the invention has excellent curative effect to rat ischemia myocardial injury; each dose group of the moldavica dragonhead dropping pill has varying degrees of protection; Composite salviae dropping pill has similar effect with the middle-dose group of moldavica dragonhead dropping pill; all dose groups of the moldavica dragonhead dropping pill have better curative effect than Yixing Badiranjibuya Keli at ischemia myocardial; dosages of middle-dose group of moldavica dragonhead dropping pill and low-dose group of moldavica dragonhead dropping pill are only 50 percent and 25 percent of the dosage of Yixing Badiranjibuya Keli respectively, thereby greatly improving the compliance of sufferers. The moldavica dragonhead dropping pill acquired from the invention is a novel preparation of convenient use and good compliance, thus bringing into play better clinical curative effect of the moldavica dragonhead, an age-old Uighur medicinal material. The production method of the moldavica dragonhead dropping pill can be carried out easily.

The invention relates to the application of Ilex latifolia total glycosides and a preparation method thereof. Studies finds that the Ilex latifolia total glycosides can reduce the content of malonaldehyde (MDA) and triglyceride (TG) in liver, increase the content of reduced glutathione (GSH) in each dose group of liver, reduce the content of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver, and has alcoholic hepatitis and fatty liver resisting activity. Compared with the prior art, the invention provides a new application of Ilex latifolia total glycosides in preparing drugs and/or functional food for preventing and/or treating alcoholic hepatitis and fatty liver; the invention also provides a preparation method of the Ilex latifolia total glycosides with the advantages of safe and simple process, saved cost, high efficiency, and easy quality control; and the invention further provides a preparation of the Ilex latifolia total glycosides.

The invention discloses a traditional Chinese medicine cancer toxin prescription for treating liver cancer and application thereof in the preparation of medicaments for resisting liver cancer. The cancer toxin prescription is prepared in accordance with the cancer toxin theory proposed by traditional Chinese medicine master Professor Zhou Zhongying, and is prepared from hedyotidis herba, muscardine silkworm, centipede, akebia fruit, pseudostellaria root, dwarf lilyturf root and rhizoma pleionis according to a certain weight ratio. The prescription has the main functions of eliminating pathogens, eliminating cancer and detoxicating and the auxiliary function of strengthening healthy qi. By using mice with liver cancer H22 transplantation tumor as materials, the pharmacological experiment result shows the cancer resistance effect of the cancer toxin prescription: the tumor inhibition rates of a low-dose group, an intermediate-dose group and a high-dose group are respectively 24.5%, 42.8% and 21.1%, thereby showing the effect of inhibiting the growth of H22 tumor mass; the tumor mass cell apoptosis rates of the low-dose group, the intermediate-dose group and the high-dose group are respectively 47.16%, 60.52% and 57.59%, thereby showing the effect of inducing tumor cells to die; and the cancer toxin prescription can block the high expression of TLR4, block the MyD88 dependent pathway of TLR4 intracellular signal transduction and inhibit the expression of downstream NF-kB proteins. Experiment shows that the cancer toxin prescription can be used for preparing medicaments for resisting liver cancer.

The invention provides a method for detecting the combination of polypeptide medicine and plasma proteins. The method comprises the following steps: marking part of the polypeptide medicine through radioactive isotopes; preparing part of the marked polypeptide medicine into a series of polypeptide medicine plasma solutions with known concentrations, quantificationally detecting the radioactivity of the polypeptide medicine plasma solutions with the known concentrations respectively, and drawing standard curves corresponding to the concentrations of the polypeptide medicine plasma solutions respectively; and preparing part of the marked polypeptide medicine into to-be-detected polypeptide medicine plasma solutions with known concentrations, performing centrifugal ultrafiltration on the to-be-detected polypeptide medicine plasma solutions, and quantificationally detecting the radioactivity of filtered solutions and unfiltered solutions, wherein the concentrations of the to-be-detected polypeptide medicine plasma solutions can be set according to plasma concentration peak values after the administration of a low dose group, a medium dose group and a high dose group in a polypeptide medicine pharmacokinetics research, so as to set the three concentrations which are approximately consistent with peak concentrations or are different from the peak concentrations with the variation amplitudes less than 10 percent.

The invention relates to the field of medicine, and especially relates to an application of thymopentin in preparing medicine used for treating mastitis. As a result of subclinical mastitis efficacy test, infection mammary area numbers of thymopentin treatment groups, especially a high dose (4mg) group, have a reduction tendency after treatment. As a result of clinical mastitis efficacy test, through injection of thymopentin (TP-5) in breast lymph node for continuously 3 days, milk SCC of two TP-5 dose groups (10mg and 20mg) both have reduction tendencies, and cow breast swellings are substantially ameliorated in appearance. Therefore, medicines prepared with high-dose thymopentin (higher than 10mg) have significant effects against cow clinical mastitis. Therefore, the medicines prepared by using thymopentin have significant effects against subclinical mastitis and/or clinical mastitis.

The invention relates to a domestic sewage treatment device, which includes a treatment box; two ends of the treatment box are respectively connected to a sewage inlet pipe and a clean water outlet pipe, and a first middle partition and a second middle partition are arranged at equal intervals in the treatment box. The first middle partition and the second middle partition divide the treatment box into a first-level treatment room, a second-level treatment room and a third-level treatment room. The aeration tube, two sets of dosing boxes are arranged on the top of the third-level treatment room, and the dosing box is connected with the third-level treatment room through the dosing pipeline; the invention is simply provided with a treatment box, and the first-level treatment room is set in the box 1, secondary treatment room and tertiary treatment room, the domestic sewage is treated three times to ensure the composite discharge standard of domestic sewage, and this device does not need special site settings, and any location with centralized sewage discharge can be used.

The invention discloses a preparation method and medicinal application of CHB which is an effective component of bupleurum Chinese and particularly relates to an application of CHB in preparation of a product for treating depression. The CHB which is the effective component of the bupleurum Chinese has a good effect on treating depression caused by various factors, has a certain protection effect on a U251 cell damaged by corticosterone and has the effects of shortening the tail-suspending immobility time and the swimming immobility time of a mouse and improving the decrease of the body temperature of the mouse due to reserpine. Each dose group is significantly and statistically different from the model group, is dose-dependent and has a good antidepressant function.

The invention relates to a polynucleotide microcapsule for treating animal disease and a preparation method thereof, belonging to the technical field of veterinary medicament preparation. Polyinosinic acid and polycytidylic acid are weighted and are added into preheated sodium chloride-phosphate buffer solution, and polynucleotide solution is prepared; the polynucleotide solution is added into ethyl cellulose to prepare soft wood and dry powder is prepared; the dry powder and magnesium stearate are added into ice-bath acetone to obtained a mixture, the mixture is stirred and slowly added into whiteruss containing 2%Span-80 surface active agent; and polynucleotide microcapsule is obtained by a method of in-liquid drying. The invention effectively prolongs the acting time of medicament within human body, decrease the applied time of the medicament and achieves the aims of long acting and sustained release. Experiments prove that the dead rate of the high dose group or the medium dose group of the polynucleotide microcapsule is obviously lower than that of an infectious control group. The cure rate is high, both are 80%, and the effective rate of the two groups are 100%. Experiments prove that the medicament of the invention is safe.

The invention provides an immunological enhancement composition and application thereof. The immunological enhancement composition comprises lycium barbarum glycopeptide LbGp4 and auxiliary materials; the mass ratio of the lycium barbarum glycopeptide to the auxiliary materials is 5: 1, and the purity of the lycium barbarum glycopeptide is greater than or equal to 90%. CY is adopted to induce chicks to generate an immunosuppression state, the antagonism of LbGp4 on immunosuppression is studied, and the result shows that the LbGp4 can remarkably improve the growth performance of the chicks induced by CY to generate immunosuppression, remarkably promote spleen development and accelerate the recovery process of spleen injury caused by CY; the multiplication capacity of CY-induced immunosuppression chick peripheral blood lymphocytes is remarkably improved, and the effect is the best when the LbGp4 medium dose group (5mg/kg.d) is adopted. According to the results, the immunological enhancement composition provided by the invention can be applied to preparation of the medicine for improving the immunity of the chicks.

The invention relates to a chemical batching component and a process of a lead-free ultra-low-sodium glass tube, in particular to a chemical batching component and a process of a lead-free ultra-low-sodium energy-saving lamp tube, and belongs to the technical field of energy-saving lamp manufacturing. It consists of SiO2, AL2O3, B2O3, K2O, Na2O, S2O, BaO, CaO, MgO, Li2O. It is characterized in that their components are as follows: SiO2 accounts for 67-68%, AL2O3 accounts for 1.5-2.0%, B2O3 accounts for 1-1.5%, K2O accounts for 10-11%, Na2O accounts for 3.5-4.0%, S2O accounts for 1.2- 2%, BaO 8-9%, CaO 2-3%, MgO 0-0.5%, Li2O 1.5-2.0%. It is characterized in that their technological process is: weighing of raw materials→stirring→feeding→melting, clarifying and homogenizing in an electric melting furnace→drawing tube forming→finely cutting round mouth of glass tube→inspecting and packing→storage. The invention has the advantages of good chemical stability, large luminous flux, high resistivity, low ultraviolet intensity, long service life, anti-aging and the like.

A method of predicting an effect of a test chemical on living organisms, which comprises:

• • a step of administering a plurality of chemicals whose effects on living organisms are known, to respective chemical dosed groups, administering a test chemical to a test chemical dosed group, collecting proteins from each group after a definite period of time, separating the proteins by two-dimensional gel electrophoresis, measuring the signal intensities of spots of the proteins and modified proteins formed therefrom, and calculating a signal intensity ratio of at least two spots, and
  • a step of comparing the signal intensity ratio of test chemical dosed group with the signal intensity ratio of each chemical dosed group.

The invention relates to itraconazole applied to treatment of gastric cancer, lung cancer and liver cancer, and a composition of the itraconazole. The daily dose of the itraconazole is 0.1-5000mg, and the itraconazole has good curative effect when the daily dose is 200mg. The itraconazole and the composition of the itraconazole disclosed by the invention have the beneficial effects that by subcutaneous injection of the itraconazole solution or the composition, the survival time of high and low-dose groups of SPF-grade Konmin mice that have liver cancer H22 can be prolonged. The itraconazole or the composition of the itraconazole has obvious inbibitional effect on Lewis lung cancer of mice in comparison with the control group according to the influence on subcutaneous tumor of the Lewis lung caner, the growth rate (P(0.01) of the tumor can be slowed down; the size (P(0.01) of the tumor is reduced; and the tumor control rate is respectively 43.0% and 45.6% after the treatment is ended. The itraconazole or the composition of the itraconazole influences positive staining of the human gastric cancer tissue VEGF (Vascular Endothelial Growth Factor) in cell cytoplasm, and the VEGF positive expression of a 0.9% sodium chloride solution group is obviously higher than that of the itraconazole group (P(0.05).

The invention belongs to the technical field of animal model construction, and discloses a drug for inhibiting myocardial hypertrophy and a construction method of a model. 60 1-day-old white feather broilers are selected and adaptively fed to an age of 10 days, and the white feather broilers are randomly divided into a blank control group, an Ang II high-dose group, an Ang II low-dose group, a nano-selenium + Ang II high-dose group and a nano-selenium + Ang II low-dose group. 15-day-old white feather broiler embryos are selected, and broiler primary myocardial cells are extracted and treated with Ang II of 2.5 * 10 <-4 > mM for 48 hours to obtain a broiler myocardial hypertrophy in-vitro model. Then, the cells are co-treated for 48 hours by adopting nano-selenium with different concentrations and Ang II with the concentration of 2.5 * 10 <-4 > mM, and the action mechanism of the nano-selenium for relieving the myocardial hypertrophy of the broilers is explored. The method can be used for researching whether biogenic nano-selenium can inhibit myocardial hypertrophy by inhibiting generation of free radicals of organisms or not.

The invention discloses application of Long Hu Ren Dan in prevention and treatment of drug-induced liver injury. The invention relates to the following steps: randomly dividing 24 mice into an APAP model group, an N-acetyl-L-cysteine positive control group (50 mg/kg), an LHRD low-dose group and an LHRD high-dose group, and additionally taking 6 mice as a normal control group; and after 6 days of administration at fixed time, performing fasting for 22 hours, injecting 250 mg/kg APAP into the abdominal cavity, killing the mice after 6 hours, and taking serum and livers of the mice. A kit is adopted to detect the serum alanine aminotransferase (ALT) activity and the glutathione (GSH) content in the livers of each group of mice; hematoxylin-eosin (H & E) dyeing is adopted to observe morphological changes of liver tissues; and Western Blot is adopted to detect expression of glutamic acid-cysteine ligase catalytic subunits and endonuclease G in the liver tissues. A fluorescent probe DCFH-DAmethod is adopted to detect the expression of reactive oxygen species (ROS) in tissue cells. The conclusion is that the LHRD can improve the acute liver injury induced by excessive acetaminophen and enhance the oxidative stress resistance of the liver.

The invention discloses a food nutrition absorption and utilization evaluation method taking sialic acid as a marker and application, and relates to detection and evaluation of food nutritional ingredients. The food nutrition absorption and utilization evaluation method comprises the following steps of: 1) dividing male ICR mice into four dose groups, namely a low dose group, a medium dose group,a high dose group and an extremely high dose group, and respectively and continuously carrying out intragastric administration on the mice; 2) collecting blood at different time points, separating theblood into plasma, precipitating plasma protein, and extracting an erythrocyte membrane; 3) detecting the content of free sialic acid, erythrocyte membrane sialic acid and protein-bound sialic acid in mouse blood; and 4) drawing a dose time concentration curve, and constructing an ordinary differential equation model of sialic acid in-vivo metabolism. An ordinary differential equation can be usedfor describing a simple and linear in-vivo metabolic process in pharmacokinetics, and a dynamic process and a change rule among studies are disclosed by establishing a relationship among variables. The food nutrition absorption and utilization evaluation method simplifies biological complexity, and can be used for evaluating and predicting the absorption and utilization of the sialic acid-containing food.

The invention discloses an experimental method for the influence of C. chinensis on the normal sexual function of male rats. The rats are randomly divided into a blank control group, a positive control group of 15 mg/kg Viagra and three dosage groups of C. kg, 1.0g crude drug/kg, 2.0g crude drug/kg, continuous gavage for 14 days, the test was carried out to determine the effect of C. chinensis on the mating ability of rats, the weight of sexual organs, the erectile function of penis, and the sperm of normal male rats. The research results of the present invention suggest that Viagra may improve the mating ability of male rats, but three doses of C. chinensis were administered orally for 28 days continuously, and the behavior of the animals was as usual, and the general condition was good. The mating ability, sexual organ index, penile erectile function, and sperm survival rate of animals in each experimental group had no obvious promoting or inhibiting effects, and there was no significant difference compared with the blank control group. Therefore, Chahuangcao has no obvious effect on the normal sexual function of male rats, and it is safe for long-term application.