Application of Long Hu Ren Dan in prevention and treatment of drug-induced liver injury

A technology for drug-induced liver injury and acute liver injury, applied in the direction of drug combinations, medical preparations containing active ingredients, and pharmaceutical formulas, which can solve problems such as restrictions on wide-scale application, scarcity of donors, and time urgency

Active Publication Date: 2020-01-03
SHANGHAI ZHONGHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the disease is not treated in time and the disease develops to liver failure, the only cure for liver failure is liver transplantation. However, due to various factors such as urgent time, scarcity of donors, and high price, its wide-scale application is limited.

Method used

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  • Application of Long Hu Ren Dan in prevention and treatment of drug-induced liver injury
  • Application of Long Hu Ren Dan in prevention and treatment of drug-induced liver injury
  • Application of Long Hu Ren Dan in prevention and treatment of drug-induced liver injury

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Specific experimental process:

[0015] 1. Animals

[0016] Thirty healthy SPF-grade C57BL / 6 male mice, 6-8 weeks old, were provided by the Guangdong Experimental Animal Center, animal production license number: SCXK (Guangdong) 2018-0034, experimental unit use license number: SYXK ( ) 2017-0125. 6 birds / cage, group breeding, free access to water and food.

[0017] 2. Drugs

[0018] Longhu Rendan (produced by Shanghai Zhonghua Pharmaceutical Co., Ltd.), acetaminophen, NAC (produced by Shanghai McLean Biochemical Technology Co., Ltd.), carboxymethylcellulose sodium (CMC-Na, Sangon Bioengineering (Shanghai) Co., Ltd. Co., Ltd.), PBS powder (Beijing Dingguo Changsheng Biotechnology Co., Ltd.).

[0019] 3. Main reagents and instruments

[0020] Anti-GCLC (PROTEINTECH GROUP), Anti-EndoG (Affinity Biosciences), Anti-β-actin (Beijing Quanshijin Biotechnology Co., Ltd.), ALT detection kit, GSH detection kit (Nanjing Jiancheng Bioengineering Research Institute Co., Ltd. ), ...

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Abstract

The invention discloses application of Long Hu Ren Dan in prevention and treatment of drug-induced liver injury. The invention relates to the following steps: randomly dividing 24 mice into an APAP model group, an N-acetyl-L-cysteine positive control group (50 mg/kg), an LHRD low-dose group and an LHRD high-dose group, and additionally taking 6 mice as a normal control group; and after 6 days of administration at fixed time, performing fasting for 22 hours, injecting 250 mg/kg APAP into the abdominal cavity, killing the mice after 6 hours, and taking serum and livers of the mice. A kit is adopted to detect the serum alanine aminotransferase (ALT) activity and the glutathione (GSH) content in the livers of each group of mice; hematoxylin-eosin (H & E) dyeing is adopted to observe morphological changes of liver tissues; and Western Blot is adopted to detect expression of glutamic acid-cysteine ligase catalytic subunits and endonuclease G in the liver tissues. A fluorescent probe DCFH-DAmethod is adopted to detect the expression of reactive oxygen species (ROS) in tissue cells. The conclusion is that the LHRD can improve the acute liver injury induced by excessive acetaminophen and enhance the oxidative stress resistance of the liver.

Description

technical field [0001] The present invention relates to a new application of Longhurendan, in particular to the application of Longhurendan in preventing and treating drug-induced liver injury. Background technique [0002] Drug-induced liver injury (DILI) refers to liver injury induced by chemical drugs, biological agents, antibiotics, traditional Chinese medicines and proprietary Chinese medicines, dietary supplements and their metabolites. Acetaminophen (APAP) is a commonly used OTC antipyretic and analgesic drug. It is safe and effective at the recommended dose. However, overdose may lead to hepatotoxicity and acute liver failure. Hepatotoxicity is the most common drug-induced liver failure in the United States and the United Kingdom. s reason. When taken according to the recommended dose, most APAP is combined with glucuronic acid or sulfate and then excreted in urine, about 5%~9% of APAP is converted into toxic active metabolite N by the cytochrome P450 system (mainly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9068A61P1/16A61K31/045
CPCA61K31/045A61K36/285A61K36/48A61K36/484A61K36/54A61K36/57A61K36/61A61K36/67A61K36/9064A61K36/9068A61P1/16A61K2300/00
Inventor 金家骅丁礼琴曹无介朱晓萍展学强陈晓兰天
Owner SHANGHAI ZHONGHUA PHARMA
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