Effective component extracted from bupleurum Chinese and application of antidepression activity thereof
An effective component, the technology of Beibupleurum, applied in the application field of antidepressant biological activity, can solve the problems of not disclosing the use of Beibupleurum for depression
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Embodiment 1
[0051] Embodiment 1: Separation of effective components of Bupleurum chinensis
[0052] 1. Materials:
[0053] 1.1 Animals: KM mice, ♂, body weight 18-22g, provided by Weitong Lihua Animal Experiment Center, certificate number: SCXK-2006-0008.
[0054] 1.2 Drugs and reagents: Fluoxetine hydrochloride (Fluooxetine, Prozac), Eli Lilly Suzhou Pharmaceutical Co., Ltd., batch number: 0724A, grind it into powder and make 3.5mg / kg suspension with water.
[0055] 1.3 Instruments and materials: HPLC, mouse tail suspension tester TU-1, provided by the Institute of Materia Medica, Chinese Academy of Medical Sciences. CONTRONIX Mouse Rectal Thermometer was purchased from Beijing Yezhiheng Technology Co., Ltd.
[0056] 2. Method
[0057] 2.1 Component extraction
[0058] 12Kg of dried Bupleurum root was crushed and extracted three times with 85% ethanol (about 140L) under reflux, and the extraction time was: 2h; 2h; 1h. The extracts were combined, concentrated and evaporated until alc...
experiment example 1
[0068] Experimental Example 1: Injury effect of CHB, an effective component of Bupleurum chinensis, on corticosterone-injured U251 cells
[0069] Glioma cell line U251, with RPMI1640 medium containing 10% inactivated FBS (add Hepes 0.2%, NaHCO 3 0.3576%) at 37℃, 5%CO 2 Cultured under certain conditions, subcultured for 3 days, and the cells in the logarithmic growth phase were taken for experiments. The complete medium adjusted the cell number concentration to 1×10 8 L -1 After inoculation in 96-well plate, 100 μL / well, 37 ° C, 5% CO 2 Cultivate overnight under the same conditions, and group the next day: add complete medium to the blank group, add corticosterone with a final concentration of 200 μmolL to the model group -1 The complete medium of the experimental group was added with CHB components (final concentrations were 10, 1mg L -1 ) and corticosterone (final concentration 200 μmol L -1 ) complete medium, each group had 3 replicate wells. 37°C, 5% CO 2 After cul...
experiment example 2
[0075] Experimental Example 2 Mouse Tail Suspension Experiment
[0076] 2.1 Drug preparation Weigh the components of Bupleurum bupleuri, and make 100mg / kg, 200mg / kg, positive drug to make 3.5mg / kg suspension.
[0077] 2.2 After 3 days of acclimatization, the mice were stratified and randomly divided into groups according to body weight, namely control group, positive group, low-dose group and high-dose group. 15 mice in each group, administered by gavage continuously for 7 days. 1 hour after the last administration, the tails of the mice were fixed on paper clips and put into the box of the tail suspension tester so that they were in an upside-down state. After 2 minutes of adaptation, start recording within 4 minutes immobility time. Judgment criteria: During the suspension period, the mouse was regarded as immobile when there was no activity.
[0078] 2.3 Statistical processing, SPSS13.0 statistical software was used for processing, and the data were expressed as "x ± s". ...
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