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Application of thymopentin in preparing medicine used for treating mastitis

A technology for mastitis and cow mastitis, applied in the field of medicine, can solve problems such as endangering human food safety, drug resistance of cow mastitis bacteria, affecting milk quality, etc.

Active Publication Date: 2013-11-13
ZHEJIANG HUAERCHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of antibiotics mainly has the following disadvantages: First, long-term use of antibiotics will produce drug resistance to cow mastitis bacteria, causing the generation of superbugs
The second is frequent use of antibiotics, once the drug is stopped, the condition will aggravate
Third, long-term use of antibiotics will affect the milk quality of milk produced by cows. Milk contains a large amount of antibiotics, which endangers human food safety.
But there is no relevant report on the application of thymopentin in the preparation of mastitis, especially cow mastitis medicine

Method used

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  • Application of thymopentin in preparing medicine used for treating mastitis
  • Application of thymopentin in preparing medicine used for treating mastitis
  • Application of thymopentin in preparing medicine used for treating mastitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Rapid diagnosis of latent mastitis in dairy cows (HMT): The first three milks are discarded prior to field sampling. Squeeze the milk from the 4 milk areas of the tested cow into the 4 chambers of the diagnostic tray, each chamber is about 2ml, and 2ml of HMT diagnostic reagent is added to each chamber. Shake the diagnostic disc to fully mix the milk and the reagents to determine the results. The criteria for determination are shown in Table 1.

[0021] Table 1 Criteria for the determination of latent mastitis in dairy cows

[0022]

[0023] Somatic cell counting method: (1) calibrate the milk somatic cell counter (Fossomatic TM Minor, Denmark) with the standard provided by FOSS; (2) heat the test milk sample (about 50 ml) in a water bath to about 40°C, The milk was mixed and tested with a somatic cell counter.

[0024] Case selection: The Jinjiang Dairy Farm in the suburbs of Hangzhou City has 120 black-and-white cows with an average age of 5.5 years. The milk sa...

Embodiment 2

[0035] Rapid diagnosis of cow's latent mastitis (HMT) next to cattle: the same as Example 1.

[0036] Somatic cell counting method: same as Example 1.

[0037] Bacteriological examination: Bacteriological examination of milk was carried out according to the procedures recommended by NMC (National Mastitis Council). The specific operation steps are as follows: (1) First, pick about 10 μL of milk sample and inoculate it into sheep blood plate medium, and cultivate at 37°C for 24-48 hours. According to the morphological characteristics of the colonies, pick suspicious colony smears, Gram staining, and microscopic examination; (2) According to the bacterial growth rate, colony morphology, and staining characteristics, it can be preliminarily divided into three categories, namely, Gram-positive cocci, Gram-positive (3) Gram-positive cocci are divided into Staphylococcus and Streptococcus by catalase test; (4) Streptococcus is divided into agalactiae according to CAMP, escin, hippu...

Embodiment 3

[0064] Selection of clinical mastitis cases: The Jinjiang Dairy Farm in the suburbs of Hangzhou has 120 black and white cows with an average age of 5.5 years. The cows with red, swollen, swollen udders and low milk production were visually screened out, and the milk samples of each cow were detected with Hangzhou Mastitis Detection Reagent (HMT). Grayish-white milk area, 15 cows in total, 15 milk areas.

[0065] Thymopentin for injection: Thymopentin provided by Zhejiang Huaercheng Bio-Pharmaceutical Co., Ltd., each containing 2 mg of thymopentin, batch number 20110401.

[0066] Grouping and treatment: 15 experimental cows were randomly divided into 3 groups: the medium and high dose groups of the experimental drug, and the blank control group, with 5 cases in each group. The three groups of patients in the experimental group were injected with thymopentin 10 mg (medium dose group) and 20 mg (high dose group) into the upper breast lymph nodes on one side of the affected breas...

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Abstract

The invention relates to the field of medicine, and especially relates to an application of thymopentin in preparing medicine used for treating mastitis. As a result of subclinical mastitis efficacy test, infection mammary area numbers of thymopentin treatment groups, especially a high dose (4mg) group, have a reduction tendency after treatment. As a result of clinical mastitis efficacy test, through injection of thymopentin (TP-5) in breast lymph node for continuously 3 days, milk SCC of two TP-5 dose groups (10mg and 20mg) both have reduction tendencies, and cow breast swellings are substantially ameliorated in appearance. Therefore, medicines prepared with high-dose thymopentin (higher than 10mg) have significant effects against cow clinical mastitis. Therefore, the medicines prepared by using thymopentin have significant effects against subclinical mastitis and / or clinical mastitis.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of thymopentin in the preparation of a medicine for treating mastitis. Background technique [0002] Mastitis is primarily an inflammation of the breast tissue caused by bacterial infection of the breast tissue. The nipple duct is a main way for bacteria to invade the mammary gland and cause infection. In a healthy state, the nipple duct epithelial cells secrete keratin and other substances with antibacterial effects, which are the first line of defense for bacteria to invade the mammary gland. When the teat duct is damaged due to milking or other reasons, the function of epithelial cells to secrete antibacterial substances is destroyed, and bacteria easily enter the mammary gland. When bacteria breach the physical barrier of the nipple, the second line of defense encountered after invading the mammary gland are the humoral and cellular defense factors in the mammary glan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61P15/14
Inventor 周正兵王祖兵贾代宏曾祖丽
Owner ZHEJIANG HUAERCHENG PHARMA
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